scholarly journals Effect of zinc on some selected blood parameters and reproductive performance of male mice

2020 ◽  
Vol 18 (2) ◽  
Author(s):  
M. G. Morshed ◽  
M. S. Hossain ◽  
Mst. G. Pervin ◽  
M. S. Haque ◽  
M. K. Islam
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Zinc Supplementation ◽  
Leukocyte Count ◽  
Sperm Count ◽  
Treated Group ◽  
Total Leukocyte Count ◽  
Male Mice ◽  
Abnormal Sperm ◽  
Group B

Background: The effect of zinc supplementation on birth weight, litter size, total erythrocyte count (TEC), hemoglobin estimation, total leukocyte count (TLC), blood glucose level, abnormal sperm count and histopathology of testis in mice was evaluated. Methods: 45 albino mice were randomly divided into 3 equal groups viz. control group, A; 10 mg zinc/kg feed treated group, B; 20 mg zinc/kg feed treated group, C. Each group was comprised of 10 male mice and 5 female mice. Then they are allowed to breed for about 25 days. After breeding males were withdrawn from each group and treated with zinc for 30 days. Then male mice were transferred back to female cage and were allowed to breed for 25 days. Results: Mice of group B and C showed a significant increase (p<0.01) in total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin content, litter size and decreased abnormal sperm count. No significant increase in body weight, even a decrease in glucose content was recorded in group B and birth weight was found increased in group C. In the histopathological study, no significant change found with different doses of zinc supplementation except reactive cell infiltration and slight tissue degeneration in the mice fed with 20 mg zinc supplement/kg feed was recorded. Conclusion: Supplementing zinc @ 10-20 mg/kg feed was found to enhance hematological parameters, increase litter size and decrease abnormal sperm count in mice.

scholarly journals Efficacy of Telmisartan in Pristane Induced Arthritis Rat Model

2021 ◽  
Vol 35 (3) ◽  
pp. 6-11
Author(s):  
Quratulain Mehdi
Keyword(s):  
Rheumatoid Arthritis ◽  
Rat Model ◽  
Leukocyte Count ◽  
Female Rats ◽  
Total Leukocyte Count ◽  
Standard Diet ◽  
Entire Study ◽  
Intergroup Comparison ◽  
Group A ◽  
Group B

Introduction: Rheumatoid arthritis is one of the most common systemic inflammatory diseases characterized by progressive damage to the joints. There is rising evidence that Renin Angiotensin Aldosterone System signaling is also involved in the inflammatory response in rheumatoid arthritis and its blockers possess anti-arthritic properties. Telmisartan is an angiotensin receptor blocker and PPAR-? agonist and its anti-arthritic effects were evaluated. Aims & Objectives: This experimental study was designed to evaluate the anti-arthritic efficacy of telmisartan in pristane induced rat model of arthritis in adult female rats. Place and duration of study: The study was conducted in the Department of Pharmacology, Army Medical College, Rawalpindi, in collaboration with National Institute of Health and Armed Forces Institute of Pathology from July 2020 to August 2020. Material & Methods: Twenty four (24) adult non-pregnant female Sprague Dawley rats were divided in three groups (n=8) designated as Group A (normal control), Group B (arthritic control) and Group C (telmisartan group) & maintained on standard diet and water adlibitum. Rheumatoid arthritis was induced in each rat of Groups B &C by a single intradermal injection of 0.5ml pristane at the base of its tail on day 0 and the disease developed in two weeks. All 3 groups were given distilled water 2.5 ml/kg from 2-4 weeks and Group C was additionally given dissolved telmisartan orally at 2 mg/kg/day. Anti-arthritic efficacy was determined by assessing arthrogram score and total leukocyte count on day 0, 14 and 28 along with histological examination done at the end of the study. Data analysis was done using SPSS version 25. Results: Healthy rats in group A maintained a unremarkable arthogram & histogram score & TLC count of 6675±350/?l during the entire study period. Telmisartan administration in Group C for two weeks after pristane induction resulted in significant reduction in arthrogram score (AS) 9.5±3.66, total leukocyte count (TLC) 7350±550/?l and histological score (HS) to 6.88±1.24 as compared to (AS) 14.50±2.07, WBC 10150±350/?L & (HS) 10.75±2.05 in Group B, left untreated with pristane alone. The intergroup comparison showed significant p value < 0.05 respectively. Conclusion: Anti-arthritic effect was shown by telmisartan as it was able to ameliorate the changes induced by pristane.

Evaluation of β-Tricalcium Phosphate Biomaterial in Femur Fracture Repair in Dogs

2021 ◽  
Author(s):  
D.K. Dwivedi ◽  
R.B. Kushwaha ◽  
M.S. Bhadwal ◽  
A.K. Gupta ◽  
J.S. Soodan ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Tricalcium Phosphate ◽  
Femur Fracture ◽  
Leukocyte Count ◽  
Weight Bearing ◽  
Fracture Repair ◽  
Total Leukocyte Count ◽  
Intramedullary Pinning ◽  
Group A ◽  
Group B

Background: Fracture repair is one of most common procedure carried out by veterinary clinicians. Fracture healing most of the time associated with delayed union, non-union and mal-uinon. Therefore, the objective of this study was to evaluate the clinical, haemato-biochemical and radiological outcome in patients after implantation of β-tricalcium phosphate as a bone graft substitute to promote the fracture healing.Methods: Eight clinical cases brought to the clinics with femur fracture were divided into two groups viz. A and B, with four animals in each group. Femur fractures were stabilized with intramedullary pinning and β-TCP biomaterial and intramedullary pinning and β-TCP plus autologous bone marrow aspirate (BMA) composite in group A and B respectively. The efficacy of fixation was studied on the basis of clinical evaluation, haemato-biochemical and radiographical parameters on day 0 and on 7th, 15th, 30th, 45th and 60th post-operative day. Result: Excellent weight bearing was noticed in group B. The overall functional outcome in group B was better in comparison to group A animals. Haematological parameters viz. haemoglobin, packed cell volume, total leukocyte count and differential leukocyte count did not differ significantly within and between the groups. The biochemical parameters viz. serum calcium increased significantly (P less than 0.05) on 7th and 15th post-operative day. A significant reduction in serum alkaline phosphatase level observed on successive post-operative days in both groups. Radiographs of fractures treated in both the groups showed good reduction and fracture fixation, early signs of fracture healing in group B than group A animals. The size of callus formation was more in group A than group B. No any graft related complications observed during the study period. The β-tricalcium phosphate facilitated fracture healing and early ambulation of affected limb.

scholarly journals Evaluation of some prescribed and over-the-counter drugs induced hemato-biochemical changes in mice

2020 ◽  
Vol 5 (4) ◽  
pp. 297-302
Author(s):  
Mohammad Rohul Amin ◽  
Shukhi Khanam ◽  
Lalin Mazumder ◽  
ABM Zunayeed Rahman ◽  
AKM Mostafa Anower ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Leukocyte Count ◽  
Male Mice ◽  
Hemoglobin Content ◽  
Animal Science ◽  
Differential Leukocyte Count ◽  
Over The Counter ◽  
Group A ◽  
Group B ◽  
Structured Questionnaire

The present study was performed in the Department of Physiology and Pharmacology, Faculty of Animal science and Veterinary Medicine, PSTU, Bangladesh. Data were collected using a semi-structured questionnaire from 125 people in Babugonj upazilla, Barishal for using drugs without prescription or at prescription. The experiment was conducted to evaluate the effects of prescribed and over-the-counter drugs on hematological (TEC, Hb and DLC) and biochemical (blood glucose) parameters in mice during the period March to April, 2019. 140 male mice were randomly divided into seven groups (A, B, C, D, E, F and G), each consisting of 20 mice. Group A was kept as control. Paracetamol, diclofenac, pantoprazole, ranitidine, diazepam and clonazepam were administered orally to mice of group B, C, D, E, F and G, respectively for 28 days. Paracetamol and ranitidine were used by 100% people followed by peantoprazole (80.80%), diclofenac-60%, diazepam-42.40% and clonazepam-24%, respectively without prescription. Paracetamol were used by 100% people followed by peantoprazole (84%), ranitidine-76%, diclofenac-66.40%, clonazepam-53.60% and diazepam 32.80%, respectively at prescription. Paracetamol and diazepam decreased significantly (p<0.05) total erythrocyte count (TEC) at 28th day. Paracetamol, diclofenac and diazepam decreased significantly (p<0.01 and p<0.05) hemoglobin content (Hb) at 28th day. The Differential leukocyte count (DLC) was not significantly changed at 28th day in mice. Paracetamol, diclofenac, pantoprazole, ranitidine, diazepam and clonazepam increased significantly (p<0.01 and p<0.05) blood glucose level in mice. It may be concluded that paracetamol is one of the most frequently used and self prescribing drugs in Bangladesh. Asian J. Med. Biol. Res. June 2019, 5(4): 297-302

scholarly journals A preliminary study on the reproductive toxicity of GS-5734 on male mice

2020 ◽  
Author(s):  
Jing Fan ◽  
Jiao Luo ◽  
Depeng Zhao ◽  
Tianqin Deng ◽  
Yuanbo Weng ◽  
...  
Keyword(s):  
Sperm Count ◽  
Reproductive Toxicity ◽  
Sperm Concentration ◽  
Sperm Parameters ◽  
Control Group ◽  
Male Mice ◽  
Abnormal Sperm ◽  
Daily Dose ◽  
Preliminary Study ◽  
Group 1

AbstractBackgroundGS-5734 as a novel and promising medicine for COVID-2019, its biological impact on the mammalian reproductive system has not been systematically studied. The aim of this study was to evaluate the effects of GS-5734 on sperm parameters and spermatogenesis in mice.Materials and MethodsIn this study, GS-5734 was synthesized according to the report. 28 adult male mice were randomly segregated into four groups (n=7 for each group). The group 1 was set as the control group, the group 1, 2, 3 and 4 were administered with GS-5734 at a daily dose of 0, 10, 50, 150 μg/mouse respectively, by intraperitoneal injection for 10 days. On the 7th day after the last injection, the testes and cauda epididymides were collected for HE staining and sperm concentration, motility, morphology analysis.ResultsThe results indicated that after treated with GS-5734, the total sperm count and motile sperm rate showed downward trends, the abnormal sperm rate showed an increasing trend. As compared with the control group, GS-5734 at a daily dose of 150 μg/mouse caused a significant decrease in sperm concentration and motility, and a significant increased of abnormal sperm rate; the 50 μg/mouse drug treatment lead to a significant decrease in sperm motility and an increase in abnormal sperm rate. The HE staining of testicular and epididymal tissues showed that the spermatogenesis of mice was significantly deteriorated with the increasing dosage of GS-5734, especially in the 150 μg/mouse group.ConclusionOur findings suggest that a high dosage of GS-5734 may induce testicular toxicity and result in deterioration of sperm parameters in mice. More investigation on the reproductive toxicity of GS-5734 is required.

Histological and Physiological Study of the Effect of Silver Nanoparticles and Omega-3 on Asthma of Male Mice Induced by Ovalbumin

2018 ◽  
Vol 9 (3) ◽  
Author(s):  
Leena Adeeb Mehdi AL-waealy ◽  
Arsha D Noori Ghani Al-Dujaili
Keyword(s):  
Silver Nanoparticles ◽  
Leukocyte Count ◽  
Histological Study ◽  
Treated Group ◽  
Protective Role ◽  
Control Group ◽  
Male Mice ◽  
Omega 3 ◽  
Animal House ◽  
Asthma Group

In the current study ninety one of male mice weighting (25-30 g) aged (15-17) weeks at the animal house faculty of science / university of Kufa during the period from January 2017 to September 2017. This study included some physiological and histological criteria to evaluate the protective role of omega-3(2 and 3 mg/kg) and silver nanoparticles (5 and 10 mg/kg) against asthma that induced by ovalbumin. The animals experimental are divided into 16 groups (n= 6 mice per each group) for duration of one and two months. The results showed significant increase (p less than 0.05) in the leukocyte count (eosinophil, neutrophil, lymphocyte, monocyte) in asthma group as compared with control group .Also ,the results showed significant decrease (p less than 0.05) in the leukocyte count in the treated group of omega-3 and silver nanoparticles for both concentration as compared with asthma group .The result sowed significant increase(p less than 0.05) in the serum level of periostin and Galectin -3 and Interleukin -33 in asthma group as compared with control group. The histological study of lung tissue revealed that induced the tissue with ovalbumin caused necrosis, degeneration and increase of mucous in bronchiole as well as acute inflammation around bronchioles while the effect of ovalbumin in trachea tissue were sluphing, necrosis and degeneration around the epithelium of bronchioles.

Effect of Hemiovariectomy on reproductive performance in Chinchila Rabbits.

2021 ◽  
Vol 36 (1) ◽  
pp. 167-174
Author(s):  
C. A. Eze ◽  
C. P. Nwodo ◽  
E. O. Ogbu
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Conception Rate ◽  
Control Group ◽  
Significant Difference ◽  
Negative Effect ◽  
Group A ◽  
Group B ◽  
The Right ◽  
Reproductive Indices

This work evaluates the effects of hemiovariectomi (37 some reproductive indices such as conception rate, birth weight and birth litter size in Chinchilla breed of rabbits. Thirty females and six males were used for the experiment. The males were examined for breeding soundness before using them to cross the females. The experiment was replicated in two batches of three groups of five females and a male each at different periods. Hemi-ovariectomy was carried out in the right ovary of 10 does (Group A) and left ovary of another 10 does (Group B). Ten females in Group C were not ovariectomised and served as the control group. All groups were served at reproductive maturity by rotating the bucks. Pregnancy was diagnosed using a standard method. The reproductive indices evaluated included conception rate, birth weight and birth litter size. Data were analyzed using percentages, ratios, and analysis of variance (ANOVA) was computed where necessary. Results showed that hemi ovariectomy significantly (P<0.01) reduced the mean litter size, Group A (2.50+58), Group B (3.00+0.71), control (Group C) (6.20+0.84). However, there was increased mean litter birth weight which, differed significantly (P<0.01) from that of the control (27.06), Group A (33.64+1.86) and Group B (33.93=1.75). Also, the conception rate in the treated groups averaged 65% while the control was 80%. There was no significant difference (P>0.05) between the groups in the interval from the introduction of the huck to the does to the time of conception. It is concluded thai hemiovariectomy has negative effect on the conception rate and birth litter size but has positive effect on birth litter weight. The procedure has shown that in highly priced breed of animal with unilateral ovarian uilments such an animal can still be used for breeding using the healthy ovary: The procedure could also be a more reliable method of birth control in pet animals as well as a means of producing heavy weight offspring.

scholarly journals Evaluation of Effect of Melatonin on Hematological Parameters in Patients of Periodontitis

2019 ◽  
pp. 46-49
Author(s):  
Anagha Marawar ◽  
Pramod Marawar ◽  
Nandal D H ◽  
Rahul Kunkulol ◽  
Sandeep Narwane
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Differential Leukocyte Count ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Root Planning ◽  
Scaling And Root Planning

Introduction: Considering the immunity enhancing property of melatonin, a study on the evaluation of the effect of melatonin on the hematological parameters in patients suffering from Periodontitis.  Methods: This study was conducted in the Department of Periodontics, Rural Dental College, Loni. Patients of chronic Periodontitis, of age between 18 to 65 years of either gender ready to give informed consent to participate in the study were included. Postoperative patients, patients having night duties, drivers and those using heavy machinery, pregnant women, lactating mothers, patients with any clinically significant systemic disease and patients on any other drugs were excluded from the study. Patients were divided into two Groups. Group B received scaling and root planning (SRP) and melatonin 3 mg per day were compared to Group A which received SRP only. The Total Leukocyte count, Differential Leukocyte count and Erythrocyte sedimentation rate of each patient was studied at a baseline, 1st (30 days) visit, 2nd (60 days) visit and 3rd (90 days) visit. Results: Both the groups consisted of 80 subjects each. Group A consisted of 71.25% male and 28.75% female patients. There were 56.25% male and 43.75% female patients in Group B. With respect to visit 3, the TLC was statistically lower in Group B. The neutrophil count of Group B was significantly lower during visit 3 as compared to Group A. The lymphocyte count of Group B was significantly lower than that of Group A during the visit 1, and also significantly higher during visit 3 on comparison with Group A. There was no statistically significant difference in the groups with respect to eosinophil and basophil count. The Monocyte count of Group B was statistically lower when compared to that of Group A. There was no statistically significant difference between ESR of Group A & Group B.  Conclusions: It can be concluded that melatonin has a positive effect on TLC and differential count of patients of periodontitis, conferring a new facet to the management of periodontitis and an attempt to impede the disease progression. Keywords: Melatonin; Hematological parameters; Periodontitis; Total Leukocyte count; Differential Leukocyte count; Erythrocyte sedimentation rate; Scaling and Root Planning.

scholarly journals EFFECT OF NERIUM OLEANDER POISONING ON BLOOD OF MALE GUINEAPIGS

2012 ◽  
Vol 3 (1) ◽  
pp. 71-73
Author(s):  
MGA Chowdhury ◽  
Azizunnesa . ◽  
MA Hossain ◽  
Q Hasan
Keyword(s):  
Body Weight ◽  
Oral Dose ◽  
Normal Level ◽  
Leukocyte Count ◽  
Nerium Oleander ◽  
Total Leukocyte Count ◽  
Maximum Increase ◽  
Group A ◽  
Haematological Changes ◽  
Group B

Haematological changes were studied in 36 adult male guineapigs during the period from July to December 1994. These animals were divided into six equal groups (A to F), each group consisting of six animals. Each animal of group B to F was administered with a single oral dose of crude watery extract of sheath oleander @ 300, 450, 600, 750 and 900 mg / kg body weight, respectively, whereas animals of group A which served as control. After administration of crude watery extract of 1/2, 6 and 72 hours a significant increase of in total erythrocyte count (TEC), total leukocyte count (TLC) and haemoglobin (Hb). The maximum increase on TEC, TLC and Hb as 35, 51 and 14%, respectively, @ 750 mg / kg body weight at 6 hr of administration of crude watery extract and the minimum value of TEC, TLC and Hb as 16, 27 and 4%, respectively, with the dose rate of 300 mg / kg body weight at the same time. The elevated haematological parameters returned to normal level within 7 days of administration.

scholarly journals Role of total leukocyte count in diagnosis of pediatric acute appendicitis: effect of antibiotic administration and duration of onset of symptoms

2017 ◽  
Vol 5 (1) ◽  
pp. 296
Author(s):  
Arun M. Ainippully ◽  
Sarath Kumar Narayanan ◽  
Vysakh C. N. ◽  
Arun Preeth V. ◽  
Prathap Somnath
Keyword(s):  
Leukocyte Count ◽  
Total Leukocyte Count ◽  
Symptom Duration ◽  
Antibiotic Administration ◽  
Group Iii ◽  
Group I ◽  
Routine Work ◽  
Number Of Patients ◽  
Group A ◽  
Group B

Background: Appendicitis is one of the most common pediatric surgical emergencies. The aim of the study is to determine the significance of total leukocyte count (TLC) in diagnosing appendicitis in the present era of rampant and injudicious antibiotic usage, and its relation to the duration of onset of symptoms.Methods: Study population consists of 148 pediatric patients who had confirmed appendicitis and underwent appendicectomy between January 2015 and December 2015. TLC was performed in all these patients as part of the routine work up. The population was categorized as: group I <24hrs, group II 24 to 4hrs and group III >48 hrs, based on symptom duration and group A and B based on antibiotics received or not respectively.Results: 64.9% (n = 96) were males and 35.1% (n = 52) were females. The ages ranged from 2.1 to 12.9 years (mean/SD being 8.3±1.2). The number of patients with elevated TLC was significantly lesser in Group B (62.2%, n = 92) when compared to the group A (37.8%, n = 56) which did not receive antibiotics (P <0.017). Significantly higher number of patients showed rise in TLC if it was done within 24 hours of onset of symptoms (66.6%, n =40).Conclusions: Our study shows that TLC proved more useful if it is performed within 24 hours of the onset of symptoms and is relevant only if antibiotics have not been administered.

scholarly journals Effect of garlic and vitamin B-complex in lead acetate induced toxicities in mice

1970 ◽  
Vol 6 (2) ◽  
pp. 203-210 ◽  
Author(s):  
MSH Khan ◽  
M Mostofa ◽  
MS Jahan ◽  
MA Sayed ◽  
MA Hossain
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Lead Acetate ◽  
Leukocyte Count ◽  
Erythrocyte Count ◽  
Total Leukocyte Count ◽  
Vitamin B ◽  
Group B ◽  
Haemoglobin Content ◽  
Serum Glutamate

The experiment was performed on 25 adult mice, (Swiss albino), weighing about 25 gm, maintained on standard pellet diet and drinking water ad libitum. All the mice were randomly divided into five equal groups (5x5). Each group comprised of five mice was marked as group A, B, C, D and E. In the present study an attempt has been taken to study the effect of Garlic and vitamin B-complex in lead induced toxicities in mice with the observation of some parameters as toxic signs and body weight, some haematological parameters like total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin content (Hb%) and Packed cell volume (PCV), some biochemical parameters such as serum glutamate pyruvate transaminase SGPT/ALT, Serum glutamate Oxaloacetate transaminase (SGOT/AST) and postmortem changes in mice. An attempt was also made to estimate the residual deposition of lead in body tissues i.e. blood, liver, kidney, brain and bone in mice. Mice treated with lead acetate showed severe toxic signs and significantly reduced total erythrocyte count, total leukocyte count, haemoglobin content and packed cell volume and significant elevation of SGPT and SGOT. But in case of mice treated by lead acetate along with Garlic and vitamin B-complex showed almost normal levels of haematological and biochemical parameters. From this experiment it is revealed that Garlic and vitamin B-complex has protective and curative effect in lead toxicity. The mean body weight of mice of group B (only lead acetate) was significantly (p < 0.01) decreased, in comparison with the other treated groups. Lead acetate group also significantly reduced (p < 0.01) total erythrocyte count, packed cell volume and hemoglobin content was on 42 days of treatment. SGPT and SGOT were significantly elevated in all treated groups in comparison with control group. The liver of the mice of group B were blackish discoloration and enlarged. The significant reduction of lead in blood, liver and kidney was found in group E < C< D in comparison to group B (lead treated). The order of effectiveness was Garlic + Vitamin B- complex > Garlic > Vitamin Bcomplex. Key words: Garlic, vitamin B-complex, lead acetate, mice   doi: 10.3329/bjvm.v6i2.2337 Bangl. J. Vet. Med. (2008). 6 (2): 203-210

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