scholarly journals Elucidation of Bioactive Potential of Two Commonly Grown North Indian Psidium guajava viz., Lalit and Shweta against Pathogenic Foodborne and MDR Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 14090-14102
Keyword(s):  
Micrococcus Luteus ◽  
Psidium Guajava ◽  
Shigella Dysenteriae ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Inhibition Of Growth ◽  
Food Borne ◽  
Phytochemical Profile ◽  
Guava Leaf ◽  
Bioactive Potential

Food-borne diseases (FBD) can cause serious health hazards. Day by day, these pathogens are becoming resistant to various antibiotics. Consequently, severe outbreaks of (multidrug resistance food-borne diseases) MDR-FBD are possible. About this, there is an urgent requirement to explore new antimicrobial compounds. As Psidium guajava L. has been in folkloric use to treat many diseases, in this study, the phytochemical profile of two North- Indian cultivars (c.v) Lalit (Pink) and Shweta (White) was elucidated, and their bioactive potential was evaluated against antibiotic-resistant FBD. Phytochemical profile relating to good antioxidant activity was exhibited by Leaf (methane, hexane, dichloromethane, and aqueous) extracts containing a good amount of flavonoid/phenolics. Putatively, they are responsible for the antibacterial potential of the extracts. As these extracts showed inhibition of growth in all the ten bacterial strains (including Staphylococcus aureus (MDR), Bacillus pumilus, Micrococcus luteus, Enterobacter epidermidis, Bacillus subtilis, Listeria monocytogens, Escherichia coli (MDR), Salmonella abony, Klebsiella pnemoneae, and Shigella dysenteriae) guava leaf extracts can be formulated as a functional food for combating MDR-FBD.

scholarly journals Antibacterial activity of GUAVA, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp, Xiphopenaeus kroyeri (Heller)

2008 ◽  
Vol 50 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Flávia A. Gonçalves ◽  
Manoel Andrade Neto ◽  
José N. S. Bezerra ◽  
Andrew Macrae ◽  
Oscarina Viana de Sousa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Enteric Bacteria ◽  
Psidium Guajava ◽  
Salmonella Spp ◽  
Leaf Extracts ◽  
Xiphopenaeus Kroyeri ◽  
Guava Leaves ◽  
Guava Leaf

Guava leaf tea of Psidium guajava Linnaeus is commonly used as a medicine against gastroenteritis and child diarrhea by those who cannot afford or do not have access to antibiotics. This study screened the antimicrobial effect of essential oils and methanol, hexane, ethyl acetate extracts from guava leaves. The extracts were tested against diarrhea-causing bacteria: Staphylococcus aureus, Salmonella spp. and Escherichia coli. Strains that were screened included isolates from seabob shrimp, Xiphopenaeus kroyeri (Heller) and laboratory-type strains. Of the bacteria tested, Staphylococcus aureus strains were most inhibited by the extracts. The methanol extract showed greatest bacterial inhibition. No statistically significant differences were observed between the tested extract concentrations and their effect. The essential oil extract showed inhibitory activity against S. aureus and Salmonella spp. The strains isolated from the shrimp showed some resistance to commercially available antibiotics. These data support the use of guava leaf-made medicines in diarrhea cases where access to commercial antibiotics is restricted. In conclusion, guava leaf extracts and essential oil are very active against S. aureus, thus making up important potential sources of new antimicrobial compounds.

scholarly journals Antimicrobial activity of Guava tree (Psidium Guajava) leaf extract and selected Commercial Antibiotics on Bacterial isolates from Kisubi Hospital

2020 ◽  
Author(s):  
◽  
David Serunjogi ◽  
Kizito Muwonge
Keyword(s):  
Antimicrobial Activity ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Leaf Extracts ◽  
Drug Resistant Bacteria ◽  
The World ◽  
Guava Leaf ◽  
Tree Leaf

Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.

scholarly journals Antimicrobial, antioxidant activity and phytochemical prospection of Eugenia involucrata DC. leaf extracts

2023 ◽  
Vol 83 ◽  
Author(s):  
A. G. Toledo ◽  
J. G. de L. de Souza ◽  
C. B. Santana ◽  
A. P. Mallmann ◽  
C. V. dos Santos ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Biological Potential ◽  
Sequestration Potential ◽  
Ic50 Value ◽  
Organic Extracts ◽  
Phytochemical Profile ◽  
Promising Source

Abstract The species Eugenia involucrata DC. is a plant native to Brazil and is traditionally used for intestinal problems, however, little research has documented about its biological potential and phytochemical profile. Thus, the objective of this study was to carry out preliminary phytochemical prospecting, antimicrobial and antioxidant potential of E. involucrata extracts. Using the E. involucrata leaves, aqueous and organic extracts were obtained using the following solvents (ethanol, methanol, hexane, acetone, dichloromethane and ethyl acetate). The phytochemical prospecting detected the presence of saponins, steroids, flavonoids and tannins in the extracts. Ethanolic and methanolic extracts presented antimicrobial activity for most of the bacterial strains tested, as well as for yeast Candida albicans, with concentrations between 3.12 and 50 mg/mL. The ethanolic and metanolic extract presented high free radical sequestration potential (>90%). The methanol extract showed an IC50 value statistically equal to that found for the commercial antioxidant BHT (p <0.05). The crude extracts obtained with ethanol and methanol were the most promising. These results suggest that methanolic, ethanolic and aqueous extracts are a promising source of natural bioactive.

scholarly journals Anticancer effect of Psidium guajava (Guava) leaf extracts against colorectal cancer through inhibition of angiogenesis

2020 ◽  
Vol 10 (7) ◽  
pp. 293
Author(s):  
AMSAbdul Majid ◽  
Bronwyn Lok ◽  
Doblin Sandai ◽  
HusseinM Baharetha ◽  
VMansoureh Nazari ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Psidium Guajava ◽  
Anticancer Effect ◽  
Leaf Extracts ◽  
Inhibition Of Angiogenesis ◽  
Guava Leaf

THE APPLICATION OF GUAJAVA LEAF EXTRACT (Psidium guajava Linnaeus) AS IMMUNOSTIMULANT OF COMMON CARP (Cyprinus carpio Linnaeus) INFECTED BY MOTILE AEROMONAS SEPTICEMIA

2021 ◽  
Vol 6 (2) ◽  
pp. 48-59
Author(s):  
Rissa Amelia ◽  
Esti Harpeni ◽  
Hilma Putri Fidyandini
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Phagocytic Index ◽  
Leaf Extracts ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Guava Leaf

This study aims to analyze the effect of the various concentration of guava leaf extract (Psidium guajava Linnaeus) on the prevention of Motile Aeromonas Septicemia disease in common carp (Cyprinus carpio Linnaeus). In a total of 150 common carp, length 9-11 cm with a density of 10 carps/aquarium. This study used a completely randomized design through 5 treatments, each treatment has 3 replications, namely (K+) consists of without guava Psidium guajava leaf extracts and without bacteria-infection Aeromonas hydrophila, (K-) consists of without guava Psidium guajava leaf extracts with bacteria-infection Aeromonas hydrophila, (A) the application of 125 ppm guava Psidium guajava leaf extracts, (B) the application of 250 ppm guava leaf extracts, (C) the application of 500 ppm guava Psidium guajava leaf extracts for 23 days. The parameters observed total of leukocytes, differensial leukocytes, hematocrit levels, erythrocyte, hemoglobin, phagocytic activity (AF), phagocytic index (IF), relative percent survival (RPS), survival rate (SR) and water quality. Data were analyzed with Anova 95% confidence interval and continued with Duncan test. The parameter also showed a significant result (P<0,05) with the application of guava Psidium guajava leaf extracts to the total leukocytes, differensial leukocytes, erythrocyte, hemoglobin, phagocytic activity, phagocytic index and relative percent survival. The results of this research indicate the concentration of 250 ppm guava Psidium guajava leaf extracts is the optimum application to increase common carp non-spesific immune responses.

Green and Sustainable Encapsulation of Guava Leaf Extracts (Psidium guajava L.) into Alginate/Starch Microcapsules for Multifunctional Finish over Cotton Gauze

2019 ◽  
Vol 7 (22) ◽  
pp. 18612-18623 ◽  
Author(s):  
Mohamed Rehan ◽  
Omar A. Ahmed-Farid ◽  
Shaimaa R. Ibrahim ◽  
Aliaa Ali Hassan ◽  
Areeg M. Abdelrazek ◽  
...  
Keyword(s):  
Psidium Guajava ◽  
Leaf Extracts ◽  
Cotton Gauze ◽  
Guava Leaf

scholarly journals Antimicrobial activity of Guava tree (Psidium Guajava) leaf extract and selected Commercial Antibiotics on Bacterial isolates from Kisubi Hospital

2020 ◽  
Author(s):  
◽  
David Serunjogi
Keyword(s):  
Antimicrobial Activity ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Leaf Extracts ◽  
Drug Resistant Bacteria ◽  
The World ◽  
Guava Leaf ◽  
Tree Leaf

Abstract Background: Plant species such as the guava have been used in Uganda and elsewhere in the world to treat some of the medical conditions associated with bacteria, this is due to the increased number of drug resistant bacteria in the world. In this study, the antimicrobial activity of guava leaf extracts against some of the disease causing bacteria isolated from Kisubi hospital in Uganda is discussed. Methods: The guava leafs were collected from a plantation near Zika forest in Uganda (0°7′27″N 32°31′32″E / 0.12417°N 32.52556°E / 0.12417; 32.52556) and samples were put in a bag and transferred to the university laboratory where they were identified. The extracts were obtained by maceration using distilled water, 30%, 50% and 70 % methanol as the extraction solvents. Antimicrobial susceptibility testing was conducted using the disc diffusion method. Results: Gram-negative Escherichia coli was sensitive to the plant extract and synthetic commercial drugs such as trimethoprim-sulfamethozole, ciprofloxacin, and Gentamicin. Pseudomonas aeruginosa was resistant to all drugs. Streptococcus pneumonia and Staphylococcus aureus were all sensitive to the plant extracts with measurable inhibition zones. Conclusion: The Guava tree leaf crude extracts have antimicrobial activity against drug-resistant bacteria. More studies should be carried out to know the potency and the concentration of different plant origin extracts.

scholarly journals Antibacterial and Cytotoxic Constituents from Bridelia verrucosa Haines Growing in Bangladesh

2017 ◽  
Vol 16 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Adeeba Anjum ◽  
Md Zakir Sultan ◽  
Choudhury M Hasan ◽  
Mohammad A Rashid
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Shigella Dysenteriae ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Inhibition Of Growth ◽  
Strong Antimicrobial Activity ◽  
Lc50 And Lc90

Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growing in Bangladesh afforded glochidonol (1), brassicasterol (2), friedelin (3), 5?-24S-ethylchoestane-3?-ol (4), stigmasterol (5), ketooleanane (6), 5-5'-dihydroxy sesamine (7) and pinoresinol (8). Compounds 1, 2, 3, 4, 6 and 8 are the first report of their isolation from the plant while compound 7 appear to be a new isolate from natural source. The isolated compounds exhibited cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90 values and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and 3 fungi. Compound 8 showed highest inhibition of growth of microorganisms with zone of inhibition of 16.7 mm against Shigella dysenteriae. Compounds 1-4 and 6-8 also revealed moderate free radical scavenging activity, in DPPH assay.Dhaka Univ. J. Pharm. Sci. 16(1): 61-68, 2017 (June)

Biocontrol of Food Spoilage Microorganisms using Leaf extracts from Magnifera indica (Mango) and Psidium guajava (Guava).

2020 ◽  
Vol 45 (4) ◽  
Author(s):  
F.O. Oseghale ◽  
K.A. Fasina ◽  
A. Ohifueme ◽  
J. Omoruyi
Keyword(s):  
Psidium Guajava ◽  
Penicillium Oxalicum ◽  
Potassium Sorbate ◽  
Food Spoilage ◽  
Leaf Extracts ◽  
Benin City ◽  
Microbiological Techniques ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Spoilage Microorganisms

The shelf life of processed food is greatly reduced by spoilage microorganisms, which has resulted in great losses to food processors, wholesalers, retailers and even consumers. This study was aimed at controlling food spoilage microorganisms using leaf extracts from mango and guava. Fried meat (beef, chicken, grass-cutter, and goat meat), fried fish and fresh bread were purchased from Benin City, and left to spoil, after which spoilage organisms were isolated, identified and characterized using standard microbiological techniques. Mango and guava leaf extracts (ethanolic and aqueous), with Potassium sorbate and sodium nitrite was used. Significant highest (<0.05) (15.67±0.88 mg/ml) antibacterial activity of Mango leaf extract was recorded against Escherichia coli, and significant highest (<0.05) antifungal activity (19.67±0.33) was recorded against Penicillium oxalicum. Ethanolic leaf extracts of mango had better MICs (6.25 mg/ml) against E. coli tested than guava (25 mg/ml). In addition, ethanolic leaf extracts from Mango did not have any effect on Bacillus polymyxa. This study suggests that extracts from Mango and Guava leaves can be explored for controlling spoilage organisms; however, ethanolic extracts are more potent than aqueous extracts.

scholarly journals BIOCIDAL POTENTIALS OF STEM BARK EXTRACTS OF PSIDIUM GUAJAVA (LINN.) ON PANEL OF BACTERIAL STRAINS ASSOCIATED WITH DIARRHEA AND DYSENTERY

2019 ◽  
pp. 84-96
Author(s):  
OLADAYO RASHEEDAT ABIDOYE ◽  
DAVID AYINDE AKINPELU ◽  
KAZEEM ADEKUNLE ALAYANDE ◽  
MAYOWA OLADELE AGUNBIADE
Keyword(s):  
Crude Extract ◽  
Stem Bark ◽  
Psidium Guajava ◽  
Shigella Dysenteriae ◽  
Dilution Method ◽  
Potassium Ions ◽  
Powdered Sample ◽  
Bacterial Strains ◽  
Aqueous Fraction ◽  
Time Kill

Objective: Investigations were carried out on cidal effects of crude extract and fractions obtained from stem bark of Psidium guajava against a panel of 74 strains of Escherichia coli and Shigella dysenteriae implicated in diarrhea and dysentery infections. Methods: Powdered sample of the stem bark was extracted in methanol/distilled water (3:2) and then partitioned into different organic solvents. The fractions obtained were subjected to antibacterial tests against a panel of bacterial strains. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using agar dilution method while the mode of action of the active fractions was investigated through time-kill dynamics and leakages of proteins and potassium ions from the cells. Results: All partitioned fractions except the aqueous fraction exhibited antibacterial activities against the panel of bacterial strains at a final concentration of 10 mg/mL. The MIC exhibited by the crude extract against susceptible bacterial strains ranged between 1.56 mg/mL and 12.5 mg/mL, while the MIC for the four fractions ranged between 0.31 mg/mL and 5.0 mg/mL. The MBC ranged between 3.13–12.5 mg/mL and 0.63–5.0 mg/mL for the crude extract and fractions, respectively. The time-kill assay revealed that the percentage of the cells killed increase with an increase in the concentrations of the fractions as well as contact time intervals. Proteins and potassium ions leakages from the bacterial cells followed the same trend with that of time-kill assay. Conclusion: The stem bark extracts of P. guajava exhibited appreciable bactericidal effects on bacterial strains associated with diarrhea and dysentery in humans.

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