scholarly journals Extraction and Determination of Nicotine in Tobacco from Selected Local Cigarettes Brands in Iraq

2021 ◽  
Vol 11 (1) ◽  
pp. 3278-3290

Tobacco smoke contains more than 3,800 different compounds, where all could harm the exposed humans to different degrees. Among them, nicotine (3-(1-methyl-2-pyrrolidinyl) pyridine) is considered the most toxic compound. In many countries, tobacco smoking is considered a severe health hazard and a significant factor in death and several common diseases. Because of that, knowing the toxicity of nicotine is important to help understand tobacco-induced human diseases and identify the potential risks associated with the therapeutic use of nicotine as an aid in smoking cessation. Thereby, estimating the nicotine amount is very crucial in tobacco production. As this work aims to extract and determine nicotine, which is the highest toxic component in tobacco plant leaves, several methods were carried out to extract the nicotine. All extracting methods were based on water extraction as a polar solvent and the other non-polar solvents. Water shows a limited extraction activity for nicotine from tobacco leaves. Since nicotine is a dibasic compound, an alkaline solution (40% NaOH) was used to extract nicotine from tobacco leaves for the selected samples. Further, solvent extraction by non-polar solvents was conducted to extract nicotine from the alkaline solution and prepare it for the spectrometric analysis. FT-IR and UV analysis show that the oily substance, which was extracted from tobacco leaves, is nicotine. Results were confirmed by microscopic examination for the extracted nicotine after the addition of mercuric chloride, where flowery shape crystals of nicotine liganded with mercuric chloride complex were formed.

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3178
Author(s):  
Tomoya Kozuma ◽  
Aki Mihata ◽  
Yoshiro Kaneko

In this study, we prepared a polyhedral oligomeric silsesquioxane (POSS)-linking polyamide (POSS polyamide) by a polycondensation of ammonium-functionalized POSS (POSS-A) and carboxyl-functionalized POSS (POSS-C) in dehydrated dimethyl sulfoxide (DMSO) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as condensing agents. The obtained POSS polyamide was soluble in various highly polar solvents, and it could form a self-standing film. FT-IR, 1H NMR, and 29Si NMR analyses showed that POSS polyamide is a polymer in which POSS-A and POSS-C are linked almost linearly by amide bonds. Furthermore, the cast film obtained by heat-treating the polymer at 150 °C for 30 min exhibited excellent transparency and hard-coating (pencil scratch test: 5H) and antifogging properties (evaluation by water vapor exposure).


e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Shahram Mehdipour-Ataei ◽  
Leila Akbarian-Feizi

AbstractA diamine monomer containing ester, amide and ether functional groups was prepared and its polymerization reaction with different diisocyanates to give main chain poly(ester amide ether urea)s was investigated. The monomer was synthesized via reaction of terephthaloyl chloride with 4-hydroxybenzoic acid and subsequent reaction of the resulted diacid with 1,8-diamino-3,6-dioxaoctane. The polymers were characterized by FT-IR and 1H-NMR spectroscopic method and elemental analysis. The resulting polymers exhibited excellent solubility in polar solvents. Crystallinity of the resulted polymers was evaluated by wide-angle X-ray diffraction (WXRD) method, and they exhibited semi-crystalline patterns. The glass transition temperatures (Tg) of the polymers determined by differential scanning calorimetry (DSC) and dynamic mechanical thermal analysis (DMTA) were in the range of 88-112 °C. The temperatures for 10% weight loss (T10) from their thermogravimetric analysis (TGA) curves were found to be in the range of 297-312 °C in air. Also the prepared polyureas showed liquid crystalline character.


2014 ◽  
Vol 70 (2) ◽  
pp. 281-287.e3 ◽  
Author(s):  
Carsten R. Hamann ◽  
Waranya Boonchai ◽  
Liping Wen ◽  
Emi Nishijima Sakanashi ◽  
Chia-Yu Chu ◽  
...  

2018 ◽  
Vol 9 (1) ◽  
pp. 39-49 ◽  
Author(s):  
Sharifah Nurul Ain Syed Hashim ◽  
Sarani Zakaria ◽  
Chin Hua Chia ◽  
Sharifah Nabihah Syed Jaafar

In this study, soda alkali lignin from oil palm empty fruit bunch (EFB-AL) and kenaf core (KC-AL) are esterified with maleic anhydride under two different conditions, namely i) pyridine at temperature of 120°C for 3h and ii) aqueous alkaline solution at room temperature for 4h. As a result, the weight percentage gain (WPG) of the esterified EFB-AL (EFB-EL) and esterified KC-AL (KC-EL) in pyridine demonstrated a higher compared to aqueous alkaline solution. The FT-IR results of EFB-EL and KC-EL in both solvents exhibited some changes at the carbonyl and hydroxyl groups. Furthermore, the esterification process induced the carboxylic peak to appear in both alkali lignin samples. The outcome is confirmed by conducting H-NMR analysis, which demonstrated ester and carboxylic acid peaks within the spectral analysis. Finally, the TGA results showed both EFB-EL and KC-EL that are exposed to aqueous alkaline actually possessed better thermal stability and higher activation energy (Ea) compared to the esterified samples in pyridine.


2020 ◽  
Vol 9 (4) ◽  
pp. 129
Author(s):  
ABDUL RACHMAN ◽  
NFN MAHFUDZ

<p>Percobaan lapang telah dilakukan pada tanah vetisol di Kebun Percobaan Pekuwon, Bojonegoro, dimulai Mei sampai September 1996, untuk mempelajari pengaruh populasi tanaman tembakau terhadap sifat agronomis dan kadar Cl daun tanaman tembakau Virginia yang diolah dengan cara dirajang. Tinggi tempat dari lahan percobaan 13 m dpi, dan dengan tipe iklim D. Tanah bertekstur liat dengan 80% liat, 15% debu dan 5% pasir, 0.62% C-organik, 0.10% N, dan pH 8.20. Percobaan disusun dalam ancangan acak kelompok dengan 4 ulangan. Perlakuan terdiri dari enam laraf populasi tanaman tembakau (12 000; 18 000; 24 000; 36 000; 48 000, dan 60 000 tanaman per ha atau setara dengan 144, 216, 288, 432, 576, dan 720 tanaman per petak). Ukuran petak 12 m x 10 m. Dalam percobaan ini akan dipelajari pula pengaruh bentuk hasil (rajangan dan krosok) terhadap kadar Cl daun. Sepuluh tanaman per petak diolah secara lue-cuing di dalam oven mini, untuk dianalisis kadar Cl daunnya. Varietas tembakau adalah DB 101. Hasil dan indeks tanaman masih terus meningkat sampai populasi tetinggi, sedangkan mutu dan rendemen tidak terpengaruh oleh peningkatan populasi. Peningkatan populasi sebaliknya menurunkan ukuran daun, bobot tiap daun, tinggi tanaman, lingkar batang, dan jumlah daun yang dapat dipanen. Berdasar hasil, mutu dan mudahnya pengelolaan di lapang populasi yang optimal adalah 24 000 tanaman per ha, untuk tembakau Virginia rajangan di Bojonegoro. Peningkatan populasi hanya berpengaruh pada kadar Cl daun atas saja. Sedangkan bentuk hasil tembakau ajangan dan krosok tidak berpengaruh pada kadar Cl daun.</p><p>Kata kunci: Nicotiana tabacum, tembakau. tembakau Virginia rajangan, populasi tanaman, vertisols, sifat agronomis, kadar Cl, bentuk olahan</p><p> </p><p><strong>ABSTRACT </strong></p><p><strong>Effect of plant population on agronomic characteristics and leaf Cl content of sliced Virginia tobacco grown in vertisols of Bojonegoro</strong></p><p>The expeiment was conducted in vetisol soil of Bojonegoro in 1996 to study the effect of plant population on agronomic characteistics and Cl content of sliced tobacco leaves. The experiment was site located 13 m above sea level, with D climatic type. The soil characteristics were clay texture with 80% clay, 15% silt and 5% sand, 0.62% C-organic, 0.10 % N, and pH 8.2. The treatment consisted of six plant populations (12.000 up to 60.000 plants per ha equal with 144, 216, 288, 432, 576, and 720 plants per plot) was arranged in a randomized block design, with four replications. Plot size was 12 m x 10 m. Plant population per plo( based on the six treatment levels. For Cl content analysis, 10 plants plot was also taken to be processed with lue-curing method in mini oven. Tobacco variety was DB 101. Yield and crop index increased steadily as plant population increased up to the highest plant population. However the increase in plant population decreased the size, weight, number of leaves, and plant height. While grade index, percentage of dry to fresh leaf yield and Cl content of lower and middle leaves were not affected by the increase in plant population. Base on yield, quality, and easier management, the recommended plant population was 24 000 plant per ha. The increase in plant population only affected the Cl content of upper leaves. The form of sliced and lue-cured tobacco leaves did not affect the Cl content of (he leaves.</p><p>Key words : Nicotiana tabacum, tobacco, sliced Virginia tobacco, plant population vetisols, agronomic characteristic, Cl contcnl, product form</p>


2019 ◽  
Vol 156 (1) ◽  
pp. 159-172 ◽  
Author(s):  
Mamy Jayne Nelly Rajaofera ◽  
Yi Wang ◽  
Zaheer Amed Jatoi ◽  
Pengfei Jin ◽  
Hongguang Cui ◽  
...  

AbstractFinding synthetic pesticide alternatives for health and a healthy environment has become a crucial issue for scientific research. A number of studies have reported efficacy of Bacillus species on promoting plant development, as well as protecting plants against pathogen invasion, especially pathogenic fungi and bacteria. However, little was known about Bacillus species in controlling viral diseases. In this study, Bacillus atrophaeus strain HAB-5, isolated from cotton field, Xinjiang, China efficiently promoted the growth of tobacco plants. According to the results, the treatment with the strain HAB-5 increased the expression of NtEXP1 and NtEXP2. Then the Tobacco mosaic virus (TMV)/ Nicotiana tobacco system was employed to evaluate virus resistance induced by strain HAB-5. Tobacco leaves were treated with antimicrobial metabolites of strain HAB-5 (1 mg/mL), and 12 h later the treated leaves were challenged with TMV via rub-inoculation. The results showed that disease symptoms were obviously compromised by tobacco leaves treated with strain HAB-5, and the viral accumulation level was reduced extensively. Moreover, it was found that the signaling regulatory gene (NPR1), defense genes (PR-1a, PR-1b, Chia5), and hypertensive response related genes (Hsr203J, Hin1) were up-regulated in plants treated with the metabolites. Altogether, these accumulated results strongly support strain HAB-5 to be a biological controlling agent against TMV.


2013 ◽  
Vol 17 (06n07) ◽  
pp. 555-563 ◽  
Author(s):  
İlke Gürol ◽  
Gülay Gümüş ◽  
Emel Musluoğlu ◽  
Yadigar Arslan ◽  
Vefa Ahsen

The synthesis of tetra and octa perfluoroalkyl substituted zinc and metal free phthalocyanines ( ZnPc and H 2 Pc ) are reported. The compounds 1–5 have been prepared by nucleophilic substitution of 4-nitrophthalonitrile with 1H,1H,2H,2H-perfluorooctanethiol, 1H,1H,2H,2H-perfluorodecan-1-ol, 1H,1H-perfluorodecan-1-ol, 1H,1H-perfluorotetradecan-1-ol and 1H,1H-perfluoro-3,6,9-trioxatridecan-1-ol, respectively. The compounds 6–8 have been prepared by the reaction of 1H,1H,2H,2H-perfluorodecan-1-ol, 1H,1H-perfluorodecan-1-ol and 1H,1H-perfluoro-3,6,9-trioxatridecan-1-ol with 4,5-dichlororphthalonitrile, respectively. The compound 9 with thia-bridge has been synthesized from 4,5-dichlororphthalonitrile. Zinc and metal free phthalocyanines were obtained from the corresponding phthalonitrile derivatives. All compounds were characterized by using mass, 1 H , 19 F NMR, UV-vis and FT-IR as well as elemental analysis. Tetra substituted Zn ( II ) phthalocyanines are slightly soluble only in THF (Compound 4a and 7a are insoluble) but metal free phthalocyanines are not soluble. Octa substituted Zn ( II ) and metal free phthalocyanine are soluble in polar solvents such as THF and DMSO.


2012 ◽  
Vol 450-451 ◽  
pp. 38-41
Author(s):  
Peng Fei Yang

The urethane reaction kinetics of 1,2-propanediol with phenyl isocyanate are investigated in different solvents, such as xylene, toluene and dimethylformamide. In-situ FT-IR is used to monitor the reaction to work out rate constant. It showsthat the urethane reaction has been found to be a second order reaction, solvents largely affects reaction rates. The reaction is largely accelerated in polar solvents, following the order of dimethylformamide > toluene > xylene. Further more, when dimethylformamide is used as solvent, the rate constants are different between initial stage and final stage, which belongs to different hydroxyls in 1,2-propanediol. However, when toluene or xylene is used as solvent, the rate constant is the same. That is, there is no reactivity difference for hydroxyls in 1,2-propanediol.


2019 ◽  
Vol 126 ◽  
pp. 00027
Author(s):  
Sergey K. Papusha ◽  
Vladimir K. Papusha ◽  
Yulia A. Pestryakova

The article is devoted to tobacco leaves harvesting in per leaf way, using a roller-type separating device acting on object of harvesting with rolling cutter having cutting flanges forming leaf–separating cells in working area.The subject of these studies is highly specialized and relates to field of agricultural engineering. Tobacco plants were chosen as an object of processing. The aim of research was theoretical substantiation of the interaction of tobacco stems with leaf–separating device in process of harvesting.The article has a research character, which is expressed in fact that theoretical analysis of interaction of tobacco plant with leaf–separating of cutter–roller type in process of harvesting is carried out. In result of research there were obtained dependences that allow to determine constructive and technological parameters of separating device for separating tobacco leaf. Conclusions set out the main results achieved so far.


2016 ◽  
Vol 14 (1) ◽  
pp. 308-315 ◽  
Author(s):  
Nikolaos Zacharopoulos ◽  
Konstantinos Koukoulakis ◽  
Evangelos Bakeas ◽  
Athanassios I. Philippopoulos

AbstractThe ruthenium(II) complex cis-[RuCl2(PPh3)2(L1)] (1) where L1 = 2-(2’-pyridyl)quinoline was obtained in high yield from the reaction of [RuCl2(PPh3)3] with L1. The new compound was characterized by different spectroscopic methods including FT-IR, UV-Vis, NMR (1H, 31P) spectroscopy along with a mass spectrometric analysis (ESI-MS) and conductivity measurements. 31P NMR spectroscopy provided evidence that the two PPh3 ligands are orientated trans to each other in an octahedral environment. Complex (1) was tested in the transfer hydrogenation of various ketones in 2-propanol at 82 oC. The catalytic activity of (1) displayed quantitative conversions for benzophenone and 4-chloroacetophenone.


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