Comparative study on antimicrobial activities of green synthesized TiO2 nanoparticles of Tephrosia purpurea leaves with its crude extracts
The present study deals with the enhancement of antimicrobial potential of Tephrosia purpurea crude extracts by making their composite with TiO2 nanoparticles against ubiquitous pathogens which may cause skin and wound infections. The synthesis was accomplished by the sol-gel method using precursor Titanium isopropoxide. The nanoparticles characterized by XRD, FESEM and FTIR techniques. XRD results showed that particles were in anatase phase with average crystallite size 12- 24 nm. FESEM reveals the spherical surface morphology of the nanoparticles. FTIR spectra gave the idea about associated functional groups with the synthesized particles. The antimicrobial potential of chemically synthesized TiO2 nanoparticles, green synthesized TiO2 nanoparticles and the crude extracts tested against Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 2474 and Streptococcus pyogenes MTCC 442 which are actively involved in skin and wound infections. Results showed that green synthesized TiO2 nanoparticles gave better results in comparison with chemically synthesized TiO2 nanoparticles and crude extracts.