scholarly journals BIOLOGI REPRODUKSI DAN TINGKAT KEBERHASILAN PEMIJAHAN IKAN BAUNG Hemibagrus nemurus (Valenciennes, 1840) POPULASI CIRATA DENGAN INKUBASI SUHU BERBEDA

2018 ◽  
Vol 13 (2) ◽  
pp. 131 ◽  
Author(s):  
Deni Radona ◽  
Jojo Subagja ◽  
Vitas Atmadi Prakoso ◽  
Irin Iriana Kusmini ◽  
Anang Hari Kristanto

Ikan baung merupakan salah satu komoditas populer di Indonesia. Dalam pengembangan budidayanya masih diperlukan input teknologi terutama pada proses pembenihan. Penelitian ini bertujuan untuk mengevaluasi karakter biologi reproduksi dan keberhasilannya dalam proses pemijahan pada ikan baung populasi Cirata yang diinkubasi pada suhu 23°C-24°C, 25°C-26°C, 27°C-28°C, dan 29°C-30°C. Inkubasi induk dilakukan pada styrofoam berukuran 45 cm x 35 cm x 25 cm dengan ketebalan 3 cm. Setiap styrofoam diisi satu ekor induk yang matang gonad. Seleksi tingkat kematangan gonad dilakukan secara kanulasi dan induksi hormon menggunakan LHRH analog (0,6 mL/kg). Penyuntikan dilakukan dua kali dengan selang waktu enam jam. Styrofoam diisi air dengan ketinggian 20 cm, dilengkapi tutup pada bagian atas, water heater, dan sistem aerasi. Inkubasi suhu dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan empat perlakuan suhu dan masing-masing perlakuan diulang sebanyak tiga kali. Hasil penelitian menunjukkan suhu optimal inkubasi pada induk yaitu 27°C-28°C dengan waktu laten 8 jam 35 menit, dan derajat ovulasi 100%. Secara statistik inkubasi induk pada suhu 27°C-28°C menunjukkan nilai karakter biologi reproduksi yang berbeda nyata (P<0,05) dibandingkan dengan inkubasi suhu 23°C-24°C dan 25°C-26°C. Nilai biologi reproduksi yang dihasilkan pada perlakuan terbaik tersebut adalah indeks kematangan gonad 8,6 ± 0,5%; fekunditas 23.909 ± 1.473 butir per ekor; derajat pembuahan 85,5 ± 5,5%; derajat penetasan 69,9 ± 5,0%; dan sintasan 72,3 ± 5,8%. Pada inkubasi suhu 27C°-28°C, telur terdistribusi dengan diameter telur rata-rata sebesar 1,5 mm.Asian redtail catfish is one of the most popular fish commodities in Indonesia. However, improvements in its aquaculture technology are still needed, especially in the breeding process. This study was aimed to evaluate the characters of reproductive biology and level of spawning on Asian redtail catfish from Cirata population incubated at different temperature settings of 23°C-24°C, 25°C-26°C, 27°C-28°C, and 29°C-30°C. Broodstock incubation was conducted in styrofoam boxes (sized 45 cm x 35 cm x 25 cm) with a wall thickness of 3 cm. One mature broodstock was placed inside each Styrofoam. Each styrofoam box was previously filled with freshwater of 20 cm deep, equipped with a lid on top, a water heater, and an aeration system. The gonad maturity stage of each broodstock was determined using cannulation, whilst the hormone induction used LHRH hormone analog (0.6 mL/kg). The injection was performed twice within six hours interval. The incubation was arranged in a completely randomized design (CRD) with four temperature treatments with three replicates. The results showed that the optimal incubation temperature for the broodstock was 27°C-28°C with the latent time ovulation of 8 hours 35 minutes and an ovulation rate of 100%. Statistically, the incubation of broodstock at 27°C-28°C showed a significant difference on the reproductive biological character value (P<0.05) compared to temperature ranges at 23°C-24°C and 25°C-26°C. The reproductive biology parameters generated were gonadosomatic index of 8.6 ± 0.5%; fecundity of 23,909 ± 1,473 egg per individual; fertilization rate of 85.5 ± 5.5%; hatching rate of 69.9 ± 5.0%; and survival rate of 72.3 ± 5.8%. At the incubation temperature of 27°C-28°C, the egg was distributed with an average egg diameter of 1.5 mm.

2021 ◽  
Vol 16 (1) ◽  
pp. 29
Author(s):  
Fajar Maulana ◽  
Dinar Tri Soelistyowati ◽  
Muhammad Fadlan Furqon

Black ghost knifefish, Apteronotus albifrons, is a South America-introduced ornamental fish species that has been widely cultivated in Indonesia. Some farmers breed this fish with different sex ratios, but the optimum sex ratio remains unclear. This study aimed to evaluate the spawning behavior and reproductive performance of black ghost knifefish with different sex ratios. The treatments in this study were arranged in a completely randomized design consisting of different sex ratios between males and females, namely: A (one male : three females), B (two males : three females), and C (three males : three females). Each treatment was done in triplicate. The broodstock were maintained in an aquarium (80 cm x 40 cm x 40 cm) and fed with bloodworm twice a day. The water was changed every day as much as 60% of the total volume. During the experiment, the parameters of spawning behavior, number of fish spawning, number of eggs, fertilization rate, hatching rate, and daily spawning frequencies were observed. The observation was done for seven days. The study results showed that black ghost knifefish spawned at night (11 pm - 2 am). The mating and spawning occurred between one male and one female. Competition between males was observed in the treatments indicated by aggressive movements of a male toward the others, such as sudden approaching, chasing, and driving away the others. Fish in treatment-B spawned consistently from day-1 to day-5. Fish in treatment-A spawned from day-2 to day-4, while fish in treatment-C spawned only on day-7. The reproductive performance parameters showed no significant difference in all treatments except the hatching rate parameter of treatment-B. The study concludes that better reproductive performance of black ghost knifefish, A. albifrons can be achieved with a spawning ratio of two males and three females. Further research on individual and mass spawning methods with the best spawning ratio of the fish is required.


Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 32
Author(s):  
Livana Dethris Rawung ◽  
Damiana Rita Ekastuti ◽  
Muhammad Zairin Junior ◽  
Min Rahminiwati ◽  
Ade Sunarma ◽  
...  

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs.  However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.


2016 ◽  
Vol 1 (3) ◽  
pp. 553-568 ◽  
Author(s):  
Md Habibur Rahman ◽  
Md Anisur Rahman ◽  
Md Mer Mosharraf Hossain ◽  
Syeda Maksuda Yeasmin ◽  
Abdulla Al Asif

Labeo bata is one of the important minor carps in Bangladesh with great demand as good table fish. The study was conducted to observe the breeding performance at different management practices in Mafatema, Rupali, Modhumoti and Anan fish hatchery and disinfection treatments of water, eggs and fry in Jessore, Bangladesh during 10 March 2014 to 15 May 2015. An improvement in broodstock nutrition and feeding has been shown to greatly improve seed production. Protein and lipid percentage of broodstock diet have been identified as major dietary factors. Protein level was 24.77%, 23.47%, 18.08%, 17.78% and lipid level was 11.07%, 9.50%, 7.74%, 8.14% in Mafatema, Rupali, Modhumoti and Anan fish hatchery respectively. Three concentrations of four chemical-formalin (10, 20, 30 mg/L), malachite green (1, 3, 5 mg/L), NaCl (1, 2, 3 g/L) and methylene blue ( 1, 3, 5 mg/L) treatment regimes and a control were compared for efficacy in treating L. bata eggs to prevent fungus and bacterial infection and improve hatch and survival rate of fry. Highest correlation value between absolute fecundity and body weight (r=.938, p<.05) and total length (r=.891, p<.05) and gonadosomatic index (26.2%) were found in Mafatema fish hatchery among four experimental hatcheries at 24.77% protein and 11.07% lipid level. Better fertilization rate (84.2±5.17%) and hatching rate (82.0±4.30%) were found in Mafatema and Rupali fish hatchery respectively that has significant difference (P<0.05) from that of Modhumoti and Anan fish hatchery at higher protein and lipid level. Lowest deformity rate (6.05+2.65) was observed in Mafatema fish hatchery that was significantly different (P<0.05) from that of Modhumoti fish hatchery. In case of disinfection treatment, methylene blue at 1mg/L bath treatment daily for 4 days showed significantly higher hatching rate (92.33±3.51%) and survival rate (94.33±4.73%).Asian J. Med. Biol. Res. December 2015, 1(3): 553-568


2018 ◽  
Vol 13 (1) ◽  
pp. 11 ◽  
Author(s):  
Samuel Lante ◽  
Andi Tenriulo ◽  
Andi Parenrengi

Udang windu transgenik merupakan udang hasil rekayasa dengan mengintroduksikan gen antivirus yang diisolasi dari udang windu untuk menghasilkan fenotipe yang lebih baik. Domestikasi udang transgenik telah dilakukan dan berhasil memijah/bertelur, tetapi umumnya telurnya infertil yang disebabkan tidak terjadinya pembuahan di tambak pemeliharaan. Udang betina tidak kawin ditandai tidak membawa spermatofor di telikumnya. Upaya untuk mendapatkan telur fertil udang dengan inseminasi buatan (IB) perlu dilakukan. Tujuan penelitian untuk mengevaluasi performa reproduksi udang betina transgenik dan mutu larva yang dihasilkan pasca IB menggunakan sumber spermatofor yang berbeda. Penelitian ini dirancang dengan tiga perlakuan yaitu: IB menggunakan spermatofor udang windu jantan transgenik (SJT), spermatofor udang windu jantan alam Sulawesi Selatan (SulSel) (SJS) dan spermatofor udang windu jantan alam Aceh (SJA). IB dilakukan pada udang windu betina transgenik setelah dua hari moulting. Hasil penelitian menunjukkan bahwa udang windu betina transgenik pasca IB perlakuan SJT menghasilkan total telur fertil sebanyak 766.949 butir, perlakuan SJS 535.644 butir dan perlakuan SJA 678.016 butir dengan daya tetas telur fertil yaitu: pada SJT, SJS, dan SJA masing-masing adalah 53,5%; 53,7%; dan 55,0%. Uji vitalitas larva dengan perendaman dalam larutan formalin 150-200 mg/L, perendaman air tawar: 5-15 menit, dan pengeringan 3-9 menit menghasilkan sintasan larva udang yang relatif sama pada ketiga perlakuan. Nilai morfologi larva perlakuan SJT, SJA, dan SJS adalah masing-masing 85,0; 84,5; dan 75,0. Dari hasil penelitian ini mengindikasikan bahwa performa reproduksi udang windu betina transgenik dan mutu larva yang dihasilkan pasca IB tidak dipengaruhi oleh sumber spermatofor induk udang windu jantan Penaeus monodon.Transgenic tiger shrimp, Penaeus monodon has been developed in the last decade to equip shrimp with immunity against viral diseases. However, the effort to produce large quantities of specific pathogen resistance (SPR) tiger shrimp seed is hampered by several constraints in the domestication process. The successfulness of domesticated broodstock in producing larvae is very low due to low fertilization rate. An artificial insemination (AI) offers a solution to increase fertilization rate in crustacean. This study was aimed to evaluate the reproductive performance of female transgenic tiger shrimp broodstock and their larval quality after artificially inseminated with males from different sources. The spermatophores of male from different sources i.e. transgenic male spermatophore (SJT), wild male from South Sulawesi (SJS), and wild male from Aceh (SJA) were collected through electric shock and inseminated to female transgenic broodstock two days after moulting. The results showed that the total numbers of fertile eggs produced from SJT, SJS, and SJA treatment were 766,949 pcs; 535,644 pcs; and 678,016 pcs, respectively and not significantly different (P>0.05). Similar to the number of fertile eggs, the hatching rate of eggs of SJT (53.5%), SJS (53.7%), and SJA (55.0%) also did not indicate any significant differences (P>0.05). On the larval vitality test by soaking the larvae in formalin and freshwater as well as by air exposure at different duration showed no significant difference on the survival rate (P>0.05) as indicated by score value at each treatment of 85.0, 84.5, and 75.0 for SJT, SJS, and SJA, respectively. In conclusion, the reproductive performance of female transgenic tiger shrimp and their larval quality were not affected by the different sources of spermatophores inseminated artificially during the spawning cycle.


2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.


Author(s):  
Selvia Oktaviyani ◽  
WANWAN KURNIAWAN ◽  
FAHMI

This study provides information on the reproductive biology of the coral catshark caught in the waters around Seribu Islands, Indonesia. A total of 257 coral catsharks were collected from February 2017 to January 2018. The total length of coral catshark ranged from 170 to 585 mm for females and 255-575 mm for males, respectively. The total weight of females ranged from 85 to 640 g and males from 100 to 620 g The sex ratio between females and males was 0.45 and it was not significantly different from the expected 1:1 ratio. The growth pattern of coral catsharks in Seribu Island was negatively allometric and the population was dominated by mature sharks. The mean size at first maturity was estimated to be 557 mm for females and 514 mm for males, based on to the relationship between the proportion between maturity stage and total length. The ovulation season was estimated occur from October to March, whereas September to April was mating season based on monthly variations of gonadosomatic index (GSI) values and the occurrence of egg cases in uterus.


DEPIK ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 235-242
Author(s):  
Mustaqim Mustaqim ◽  
Kartini Eriani ◽  
Erlangga Erlangga ◽  
Rachmawati Rusyidi

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak.  Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis


2021 ◽  
Vol 26 (1) ◽  
pp. 17
Author(s):  
Vinka Leonita ◽  
Deny Sapto Chondro Utomo ◽  
Hilma Putri Fidyandini

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.


2021 ◽  
Vol 1 (1) ◽  
pp. 23-28
Author(s):  
Yuni Mariani ◽  
M Ayatullah Hamzani

This study was intended to determine the best temperature for hatching free-range chicken eggs in the incubator so that breeders could get more day-old chicken (DOC) with the same diversity. The research design used a completely randomized design (CRD). This study used 150 free-range chicken eggs obtained from breeders in Gunung Paok Hamlet, Perian Village, Montong Gading District, East Lombok Regency, which were divided into three treatments, namely 35 ° C - 36 ° C, 37 ° C - 38 ° C and 39. ° C - 40 ° C and five repetitions with the number of eggs repeating using 10 free-range chicken eggs. The variables observed were fertility, mortality, and hatchability of native chicken eggs. Data analysis was performed using Analysis of Variance (ANOVA). The results of the analysis of variance showed that differences in the temperature of native chicken eggs in the incubator showed a significant difference (P> 0.05) in fertility, mortality, and hatchability. The best results were obtained at an incubation temperature of 37 ° C - 38 ° C with an average hatchability of 82,88± 8,87%.


2019 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Vina Violita ◽  
Muslim Muslim ◽  
Mirna Fitrani

AbstrakPenetasan telur ikan merupakan hasil dari embriogenesis hingga embrio keluar dari cangkangnya. Aktivitas embrio dipengaruhi oleh faktor dari luar dan dalam cangkang. Salah satu faktor dari luar yaitu pH air. Tujuan dari penelitian ini adalah untuk mengetahui derajat penetasan dan lama waktu menetas telur ikan betok (A. testudineus) yang diinkubasi pada media dengan pH berbeda. Penelitian ini telah dilakukan di Laboratorium Budidaya Perairan, Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya. Metoda penelitian menggunakan rancangan acak lengkap (RAL) dengan lima perlakuan dan tiga ulangan P1 (pH 5±0,2), P2 (pH 6±0,2), P3 (pH 7±0,2), P4 (pH 8±0,2) dan P5 (pH 9±0,2). Parameter yang diamati yaitu persentase telur menetas, lama waktu penetasan dan parameter kualitas air. Hasil penelitian menunjukkan bahwa pada pH 7±0,2, pH 8±0,2 dan pH 9±0,2  memberikan hasil persentase telur menetas dan lama waktu penetasan telur yang tidak berbeda nyata.AbstractThe hatching of fish eggs is the result of embryogenesis until the embryo out from the shells. Embryos activities are influenced by external and internal factors. One of the external factors is the Power of Hydrogen (pH). The purpose of this study is to determine the hatching rate and incubation time of climbing perch (Anabas testudineus) incubated on different pH of the medium. The research had been conducted in Aquaculture Laboratory, Department of Aquaculture, Agriculture Faculty, Sriwijaya University. The research method used a completely randomized design with five treatments and three replications.  The treatments were P1 (pH 5±0.2), P2 (pH 6±0.2), P3 (pH 7±0.2), P4 (pH 8±0.2) and P5 (pH 9±0.2). Parameters that had been observed were the eggs percentage of hatching, incubation time and water quality parameters. The results showed that pH 7±0.2, pH 8±0.2 and pH 9±0.2 have no significant difference on hatching percentage and incubation time of climbing perch.


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