scholarly journals Microbial analysis of tap water collected from selected upazillas of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh

2018 ◽  
Vol 4 (2) ◽  
pp. 193-200
Author(s):  
Hosneara Champa ◽  
SM Lutful Kabir
Keyword(s):  
Antimicrobial Susceptibility ◽  
Water Samples ◽  
Tap Water ◽  
Geometric Mean ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Plate Count ◽  
Rrna Gene ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern

The present study was undertaken to analyze the microbial quality of tap water samples obtained from different upazilla markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts. For achieving the above mentioned objectives, 20 tap water samples were collected and the methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated E. coli from tap water samples were characterized by using biochemical test, molecular method and antimicrobial susceptibility tests. HPC was highest in market tap water collected from Sarishabari of Jamalpur district and TCC was highest in market tap water collected from Durgapur of Netrokona district. The geometric mean of HPC of Jamalpur, Tangail, Kishoreganj and Netrokona districts water were 4.2x107, 2.7x107, 2.4x107 and 4.4x107 CFU/ml respectively. Out of 13 isolates of E. coli, 13 isolates were amplified by using 16S rRNA gene based PCR. The antimicrobial susceptibility pattern showed that the isolates were highly resistant to amoxicillin. Most of the E. coli isolates were susceptible to tetracycline, azithromycin, streptomycin, norfloxacin and ciprofloxacin. Furthermore, a few E. coli isolates were intermediate resistant to erythromycin and gentamycin. Moreover, out of 13 E. coli isolates 2 (15.4%) isolates were detected as multidrug resistant. This study indicated the presence of multidrug resistant E. coli isolates in tap water in Jamalpur, Tangail, Kishoreganj and Netrokona districts that warrant particular attention.Asian J. Med. Biol. Res. June 2018, 4(2): 193-200

scholarly journals Bacteriological assessment of tap water collected from different markets of Mymensingh, Gazipur and Sherpur districts of Bangladesh with special focus on the molecular detection and antimicrobial resistance of the isolated Escherichia coli

2018 ◽  
Vol 2 (1) ◽  
pp. 21-28
Author(s):  
Md Shihab Hassan ◽  
SM Lutful Kabir ◽  
Yousuf Ali Sarker ◽  
Md Tanvir Rahman
Keyword(s):  
Antimicrobial Susceptibility ◽  
Water Samples ◽  
Tap Water ◽  
Geometric Mean ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Plate Count ◽  
Special Focus ◽  
Rrna Gene ◽  
E Coli

The objectives of this study were to assess the bacteriological quality of tap water samples obtained from different markets of different upazillas of Mymensingh, Sherpur & Gazipur district. For achieving the above mentioned objectives, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated E. coli from tap water samples were characterized by using biochemical test, molecular method and antimicrobial susceptibility tests. HPC was highest in market tap water collected from Kaligonj and TCC was highest in market tap water of collected from Mymensingh sadar. The geometric mean of HPC of Mymensingh, Gazipur and Sherpur districts water was 8.4x105, 2.5 x106 and 6.8 x105 C.F.U/100 ml. All isolates of E. coli (n=20) were amplified by using 16S rRNA gene based PCR. In respect to antimicrobial susceptibility testing, most of the E. coli isolates were susceptible to norfloxacin, ampicilin, tetracycline, streptomycin and ciprofloxacin. Furthermore, a few E. coli isolates were intermediate resistant to gentamycin and ciprofloxacin. However, a few of the E. coli isolates were resistant to erythromycin and amoxycilin. Moreover, out of 20 E. coli isolates 3 (15%) isolates were detected as multidrug resistant. This study indicated the presence of multidrug resistant E. coli isolates in tap water in Mymensingh, Sherpur and Gazipur districts that warrants particular attention. Asian Australas. J. Food Saf. Secur. 2018, 2(1), 21-28

scholarly journals Comparative Microbiological Assessment of Drinking Water Collected from Different Areas of Kathmandu Valley

2019 ◽  
Vol 6 ◽  
pp. 39-43
Author(s):  
Aman Thapa Magar ◽  
Mamata Khakurel ◽  
Shree Laxmi Pandey ◽  
Kalyan Subedi ◽  
Umesh Kaji Manandhar ◽  
...  
Keyword(s):  
Ground Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Tap Water ◽  
Bacterial Load ◽  
Total Coliform ◽  
Bottled Water ◽  
Plate Count ◽  
Aerobic Plate Count ◽  
Filtration Technique

Objectives: This study was aimed to evaluate different water samples in terms of Total bacterial load and Total Coliform Load in comparison with different parameters such as pH, type of samples, chlorination status, turbidity, temperature and collection areas. Methods: Altogether 250 water samples were collected; 110(44%) were ground water samples, 60(24%) were public tap water samples and 80(32%) were bottled water samples. Total Coliform load was evaluated using Membrane Filtration Technique and Total Bacterial Load was evaluated using Aerobic Plate Count Technique. pH was measured using pH meter, turbidity was measured using nephelometer, temperature was measured using temperature probe and bottled water were considered chlorinated. Results: Among these 250 samples, the highest mean bacterial load was seen in public tap water samples (137×103 cfu/ml) and least mean bacterial load was seen on bottled water sample (28×103). Similarly, highest mean coliform load was seen on ground water samples (81 cfu/ml) and least mean coliform load was seen on bottled water samples (6 cfu/ml). Conclusion: This study has concluded that ground water samples contain large number of coliforms which suggest it might be fecally contaminated. Also the result had shown even bottled water contained coliforms. Therefore, it is recommended to treat ground water before using. Proper care must be taken during manufacture of bottled water.

scholarly journals Escherichia coli O157:H7 in Retail Lettuce (Lactuca sativa) in Addis Ababa City: Magnitude of Contamination and Antimicrobial Susceptibility Pattern

2021 ◽  
Vol 12 ◽  
Author(s):  
Aklilu Feleke Haile ◽  
Silvia Alonso ◽  
Nega Berhe ◽  
Tizeta Bekele Atoma ◽  
Prosper N. Boyaka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Addis Ababa ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern

Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen.

scholarly journals Qualitative and Bacteriological Assessment of Commercially Available Bottled Water in the City of Mymensingh, Bangladesh

2013 ◽  
Vol 1 (2) ◽  
pp. 81-85 ◽  
Author(s):  
Shabnam Sharmin ◽  
SM Lutful Kabir ◽  
M Mufizur Rahman
Keyword(s):  
Water Quality ◽  
Tap Water ◽  
Geometric Mean ◽  
Total Coliform ◽  
Bottled Water ◽  
Qualitative Assessment ◽  
Plate Count ◽  
The City ◽  
Good Number ◽  
Safety Guidelines

Bottled water has become one of the most popular drinks in Bangladesh. Now a days it is easily available in the market. The objectives of this study were to assess the overall quality (qualitative and bacteriological) of bottled water samples in the city of Mymensingh, Bangladesh. For achieving the objectives, standard questionnaire, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Qualitative assessment of bottled water indicated that a good number of people preferred bottled water to tap water. The criterion in order to qualify for a ‘good bottled water’ was the taste (43.3%). The bottled water quality was satisfactory in 40% of the responders in this study on the basis of peoples’ satisfactions, perceptions and expenditures on bottled water quality. On the other hand, the percentage of dumping of bottled water after consumption refuse was 33.3%. Monthly expenditure on bottled water was less than 300 taka in 80% of the responders. The geometric mean of HPC of different brands of bottled water ranged from 2.9x103 cfu/100 ml to 6.2x103 cfu/100 ml. In addition, the geometric mean of TCC of different brands of bottled water ranged from 9 cfu /100 ml to 43 cfu /100 ml. The results indicate that most of the bottled water studied were out of their safety guidelines. Recommendations for better treatment systems for bottled drinking water to address the issue appears to be essential. DOI: http://dx.doi.org/10.3329/mh.v1i2.14096 Microbes and Health, 2012 1(2): 81-85

scholarly journals Comparison of Microbiological and Physicochemical Quality of Tap and Dispenser Water from Different Mid-Range Restaurants of Dhaka City, Bangladesh

2016 ◽  
pp. 69-72 ◽  
Author(s):  
Nur Alam ◽  
Rubayat Jamal ◽  
SM Sabbir Alam ◽  
Mohammad Moniruzzaman
Keyword(s):  
Water Samples ◽  
Pathogenic Bacteria ◽  
Tap Water ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Faecal Coliform ◽  
Quality Analysis ◽  
Health Concern ◽  
Plate Count ◽  
Physicochemical Quality

Dhaka is the most populous city in Bangladesh and the tenth-largest metropolitan in the world with about twelve million inhabitants. Most people of Dhaka have low or middle ranged incomes and frequently use roadside mid range restaurants to take their meals and drinking water. Water quality is a significant health concern in Bangladesh and is a probable source of many water borne diseases and outbreaks. Tap water and dispenser water samples were collected from ten roadside restaurants. These samples were analyzed for pH, salinity, conductivity, TDS (total dissolved solid) for physicochemical quality analysis while total coliform, faecal coliform and total heterotrophic bacterial counts were determined for microbiological quality analysis. All the samples conformed to the WHO standards of TDS, salinity and conductivity. Except for 20% of the tap water samples, pH of all the samples was found to be satisfactory. The heterotrophic plate count was in a range of 8.0 x 101 cfu/ml to 9.3 x 105 cfu/ml from tap water sample and 6.5 x 101 cfu/ml to TNTC from dispenser water samples. Only 10% of the tap water samples and 30% of the dispenser water samples complied with the WHO stipulated limit of total heterotrophic count (100 cfu/ml). In terms of total coliforms, all the tap water samples and 20% of the dispenser water samples were found to be contaminated, whereas 50% of both the tap water and dispenser water samples were contaminated with faecal coliforms, indicating possible faecal contamination and presence of pathogenic bacteria. By comparing coliform/faecal coliform count on tap and dispenser water it was found that dispenser water is safer than tap water.Bangladesh J Microbiol, Volume 31, Number 1-2,June-Dec 2014, pp 69-72

scholarly journals Yeasts and filamentous fungi in bottled mineral water and tap water from municipal supplies

2007 ◽  
Vol 50 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Mirian Ueda Yamaguchi ◽  
Rita de Cássia Pontello Rampazzo ◽  
Sueli Fumie Yamada-Ogatta ◽  
Celso Vataru Nakamura ◽  
Tânia Ueda-Nakamura ◽  
...  
Keyword(s):  
Filamentous Fungi ◽  
Water Samples ◽  
Mineral Water ◽  
Tap Water ◽  
Health Hazard ◽  
Total Coliform ◽  
Species Level ◽  
Potential Health ◽  
Potential Health Hazard ◽  
Bottled Mineral Water

The main objective of this study was to analyse the occurrence of yeasts and filamentous fungi in drinking water as well as to investigate their correlation with the indicator bacteria of faecal pollution. Yeasts were detected in 36.6% and 11.6% of the bottled mineral on water dispensers and tap water samples from municipal system, respectively. Twenty-one (35.0%) of bottled mineral water and two (3.3%) of tap water samples were positive for filamentous fungi. For bottled mineral water 12 (20.0%) of 60 samples were positive for total coliform, compared with 3(5.0%)out of 60 samples from tap water. The mineral water from dispensers was more contaminated than tap water. Strains belonging to the genera Candida identified to the species level were C. parapsilosis, C. glabrata and C. albicans. Thus, bottled mineral water from water dispensers and tap water could be considered a possible transmission route for filamentous fungi and yeasts, and could constitute a potential health hazard, mainly to immunocompromised indivuals.

scholarly journals Microbiological evaluation of lettuce produced by conventional and organic systems in farms of Londrina, PR

2017 ◽  
Vol 38 (1) ◽  
pp. 175 ◽  
Author(s):  
Natalia Harumi Niguma ◽  
Jacinta Sanchez Pelayo ◽  
Tereza Cristina Rocha Moreira de Oliveira
Keyword(s):  
Irrigation Water ◽  
Water Samples ◽  
Total Coliform ◽  
Organic Production ◽  
Conventional System ◽  
Salmonella Spp ◽  
Organic System ◽  
E Coli ◽  
Organic Systems ◽  
Production Practices

The aims of this study were to evaluate the contamination of lettuce (Lactuca sativa), produced in Londrina, Paraná (PR), with total coliform, coliform at 45 °C, E. coli, and Salmonella spp.; and to determine the E. coli contamination of irrigation water used at the farms studied. Four farms were evaluated, of which three produced lettuce using a conventional system and one using an organic system. An evaluation of the production practices of the farms was also carried out. A total of 111 samples were analyzed, 71 lettuce samples from the conventional system and 40 samples from the organic system. A total of eight irrigation water samples were collected for analysis. Coliform at 45 °C counts above the limit tolerated by Brazilian legislation were observed in 2.8% (2/71) of conventionally grown lettuce samples, and Salmonella spp. was isolated in 1.4% (1/71) of those samples. In the organic lettuce samples, 12.5% (5/40) had coliform at 45 °C counts above the limit tolerated and Salmonella spp. was not detected. Irrigation water samples from three farms were unsatisfactory, with counts higher than 102MPN of E. coli per 100mL. The results of this study demonstrate that most conventionally grown lettuce samples show good sanitary conditions in production, and that lettuce contamination is not related to contamination found in irrigation water samples. The results also showed that the organic production practices required by Brazilian certification agencies should be applied to ensure that contamination of produced lettuce remains controlled.

scholarly journals Antibiogram Profiling and Thermal Inactivation of Staphylococcus aureus and Escherichia coli Isolated from Milk of Dharan, Nepal

2020 ◽  
pp. 81-87
Author(s):  
Susmita Phattepuri ◽  
Prince Subba ◽  
Arjun Ghimire ◽  
Shiv Nandan Sah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Thermal Inactivation ◽  
Total Coliform ◽  
Plate Count ◽  
Diffusion Method ◽  
E Coli ◽  
Coliform Count ◽  
Z Value ◽  
D Values

Milk is an excellent medium for the growth of many bacteria. This study aimed to determine antibiotic profiling and thermal inactivation of Staphylococcus aureus and Escherichia coli isolated from raw milk of Dharan. Total viable count, total Staphylococcal count, and total coliform count were carried out by conventional microbiological methods. Identification was done on the basis of Gram staining and biochemical tests. The antibiotic susceptibility test of the isolates carried out by the modified Kirby-Baur disc diffusion method. Thermal inactivation of S. aureus and E. coli were carried out by subjecting to thermal treatment in a water bath. Total plate count ranged from 204×104 CFU/mL to 332×105 CFU/mL. Total staphylococcal count and total coliform count ranged from 14×105 CFU/mL to 8×106 CFU/mL and 11×104 CFU/mL to 3×106 CFU/mL respectively. S. aureus showed an increasing resistance patterns towards Ampicillin, Cefotixin, Carbenicillin and Cefotaxime. Ciprofloxacin, Erythromycin, Amikacin, Gentamycin, Azithromycin, and Chloramphenicol were found to be effective against S. aureus. All the E. coli isolates were resistant to Ampicillin and least resistant to Cefotixin. Chloramphenicol, Amikacin, Azithromycin, and Nalidixic acid were found highly effective to E. coli. The D-values for S. aureus at 56°C, 58°C and 60°C were 1.36 min, 1.19 min, and 1.09 min respectively. The Z-value was 14.92°C. While D-values were obtained as 0.98 min, 0.75 min, and 0.57 min for E. coli at 56° C, 58° C and 60° C respectively, and Z-value was 9.75° C. Hence, S. aureus was found to be more heat resistant than E. coli.

scholarly journals Frequency and antimicrobial susceptibility pattern of hospital isolates of Escherichia coli and Klebsiella pneumoniae in urine samples

2019 ◽  
Vol 49 ◽  
Author(s):  
Ivana Jukić ◽  
Danijel Topić ◽  
El-Jesah Đulić ◽  
Amela Dedeić- Ljubović
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Public Health Problem ◽  
Urine Samples ◽  
Distribution Analysis ◽  
Susceptibility Pattern ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern ◽  
Urinary Infections ◽  
Tract Infections

Objectives/Aim: Urinary tract infections (UTI) are a serious public health problem and caused by many pathogens, most often by Escherichia coli and Klebsiella pneumoniae.Aim of this study was to show the frequency and antimicrobial susceptibility pattern of E. coli and K. pneumoniae in hospital isolates, following the GLASS methodology.Methods: This is a retrospective study that was conducted in the Laboratory for Urinary Infections OU Clinical Microbiology CCUS in the period from January till December 2018.A total of 13760 urine samples were processed, using standard laboratory methods, in which significant bacteriuria was detected in 3218 (23.4%) of specimens.Results: Out of the total number of positive samples, E. coli was isolated in 1166 (36.2%) and K. pneumoniae at 341 (10.6%) patients. The presence of E. coli and K. pneumoniae isolates is dominant in females (1103/73.2%) and age group 60 and older (812/54.7%). Out of the total E. coli isolates, the ESBL strain was presented with 79 (6.8%) and K. pneumoniae with 145 (42.5%). The proportion of carbapenemase (CPE) produced by K. pneumoniae isolates was 8 (2.4%). The ESBL strain distribution analysis on clinics showed the highest prevalence of both isolates at the Clinic for Nephrology: K. pneumoniae 26/17,9%; E. coli: 12/15.4%. Isolated E. coli showed the highest resistance to ampicillin 673/1166 (57,7%), trimethoprim-sulfamethoxazole 454/1166 (38.9%) and ciprofloxacin 253/970 (26.1%), while K. pneumoniae to ciprofloxacin and trimethoprim-sulfamethoxazole with 151/285 (53.0%) and 164/341 (48.1%), respectively.Conclusions: Our study has shown that the most common cause of urinary infections in hospital settings are E. coli and K. pneumoniae. Data analysis showed that the presence of ESBL isolates was significantly higher in K. pneumoniae than E. coli. CPE isolates of K. pneumoniae were also detected.

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