scholarly journals Kaji Banding Kualitas Semen Segar Empat Genetik Ayam Lokal Indonesia (COMPARATIVE STUDY ON THE QUALITY OF FRESH SEMEN OF FOUR GENETIC LOCAL CHICKEN IN INDONESIA)

2019 ◽  
Vol 20 (3) ◽  
pp. 397
Author(s):  
Junaedi Junaedi ◽  
Husnaeni Husnaeni
Keyword(s):  
Comparative Study ◽  
Spermatozoa Motility ◽  
Genetic Groups ◽  
Randomized Design ◽  
Semen Volume ◽  
Completely Randomized Design ◽  
Local Chicken ◽  
Fresh Semen ◽  
Better Than

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.

scholarly journals Karakteristik dan kualitas semen sapi Sumba Ongole dalam pengencer BTS yang dimodifikasi dengan susu kedelai

2021 ◽  
Vol 2 (2) ◽  
pp. 64-73
Author(s):  
Alphonsius Umbu Jara Lodu ◽  
Alexander Kaka ◽  
Iven Patu Sirappa
Keyword(s):  
Sperm Quality ◽  
Sperm Concentration ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Semen Volume ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Live Sperm ◽  
Fresh Semen

This study aimed to find out the quality of fresh semen from Sumba Ongole (SO) bulls which was diluted with Bestvile Thawing Solution (BTS) diluent modified with soybean milk (SKD). The study used a completely randomized design consisting of four treatments and 5 replications, P0 (100 % BTS), P1 (95 % BTS + 5 % SM), P2 (90 % BTS + 10 % SM), and P3 (85 % BTS + 15 % SM). The parameters observed were the colour, smell, consistency, pH, volume, motility, viability, and abnormalities of spermatozoa. The results showed that the characteristics were in the normal, creamy white semen colour, semen volume 3,6 ml, medium consistency, pH 6,3, typical SO bulls smell, sperm motility 85 %, mass activity +++, sperm concentration 1276 million/ml, live sperm 90,05 % and sperm abnormalities 6,15 % and quality there was no significant difference between the four treatments. The spermatozoa motility and viability in each treatment was able to which achieved on day 1. The characteristics of the spermatozoa of SO bulls were in the normal category. Meanwhile, BTS diluent with the addition of SKD was unable to maintain sperm quality.

scholarly journals KUALITAS MOTILITAS DAN VIABILITAS SPERMATOZOA DARI SEMEN AFKIR SAPI LIMOUSIN PADA PENGENCER SUSU SKIM KUNING TELUR SITRAT DENGAN PENAMBAHAN BERBAGAI KADAR GLUKOSA

2020 ◽  
Vol 7 (2) ◽  
pp. 96
Author(s):  
Rudi Irvanto ◽  
Hardijanto Hardijanto ◽  
Widya Paramita ◽  
Suherni Susilowati ◽  
Tita Damayanti L ◽  
...  
Keyword(s):  
Research Design ◽  
Glucose Level ◽  
Skim Milk ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Design ◽  
Completely Randomized Design

Quality of spermatozoa motility and viability from rejected limousin bull semen diluted with skim milk egg yolk sitrat added with various levels of glucose. Glucose level used were 0%, 0,5%, 1,5%, 2,5%, and 3,5%. Writer was using on four years old Limousine bull. Bull semen used in this research was bull rejected semen with bellow 70% motility. Semen observation was done at 0 hour, 24 hours and 48 hours. Research design used in this study was completely randomized design with faktorial pattern with 5 replicates. Highest result in motility this research was showed at 24 hours with 30% value in glucose 2,5% treatment and 48 hours with 10% value. The lowest result showed in glucose 0% treatment at 24 hours and 0% at 48 hours. Highest result in viability showed on glucose 2,5% treatment with 62,6% value at 24 hours and at 48 hours with 53,4% value. Lowest result in viability showed on glucose 0% treatment with 44,2% value at 24 hours and 31,4% value at 48 hours.

scholarly journals Penambahan PGF2α dalam pengencer semen dapat meningkatkan motilitas pasca thawing motility spermatozoa domba Waringin

2021 ◽  
Vol 19 (2) ◽  
pp. 210
Author(s):  
Husnurrizal Husnurrizal ◽  
Ade Syahriani Aritonang ◽  
Tongku Nizwan Siregar ◽  
Teuku Armansyah ◽  
Hafizuddin Hafizuddin
Keyword(s):  
Analysis Of Variance ◽  
Freezing Process ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Additional Amount ◽  
Completely Randomized Design ◽  
Artificial Vagina

<p class="MDPI17abstract"><strong>Objective: </strong>The purpose of this research was to find out the rise of spermatozoa motility of Waringin sheep which was given additional amount of PGF2α in commercial semen diluents (Andromed).</p><p class="MDPI17abstract"><strong>Methods: </strong>A total of 3 Waringin sheep aged 2-3 years were used in this study. Semen samples were collected using an artificial vagina and evaluation of the quality of the spermatozoa was carried out macroscopically and microscopically. This study used a unidirectional completely randomized design. After evaluated, semen samples were added with Andromed diluents afterwards divided into three groups treatment, K1; K2; and K3 where each group was then added with physiologic NaCl, 37,5 µg PGF2α, and 75 µg PGF2α. All of the samples were equilibrated in refrigerator for 4 hours and spermatozoa motility was examined, then after the freezing process was carried out and stored for 24 hours, the post thawing spermatozoa motility was examined. The data obtained was analyzed using one-way analysis of variance (ANOVA) and followed by Duncan test.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result of the research showed that motility of post equilibration spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 43,67±1,20; 75,00±4,00; and 82,33 ±3,18 (P&lt;0,05); and motility of post thawing spermatozoa (%) of Waringin sheeps at K1; K2; and K3 were 25,33±1,85; 49,33±3,17; dan 54,00 ±5,50 (P&lt;0,05).<strong></strong></p><strong>Conclusions: </strong>In conclusion that was the rise in motility of prefreezing spermatozoa and motility of post thawing spermatozoa of Waringin sheep which given additional amount of PGF2α on Andromed diluents.

scholarly journals Motility, Viability, Spermatozoa Abnormality, and pH of Bali Cattle Semen in Another-Yellow Water Driller Stored in a Different Time

2021 ◽  
Vol 3 (2) ◽  
pp. 76-90
Author(s):  
Fransiskus X. Manehat ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Positive Impact ◽  
Egg Yolk ◽  
Sugarcane Juice ◽  
Spermatozoa Motility ◽  
Average Value ◽  
Cane Juice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Bali Cattle

This study aims to determine the quality of Bali cattle spermatozoa diluted using sugarcane juice-egg yolk. This research was carried out in the stables and Laboratory of the Faculty of Agriculture, University of Timor in August-September 2020. Semen was collected from an adult male bali cattle, aged ± 4.5 years in healthy condition. The method used in this study is an experimental method using a completely randomized design consisting of 4 treatments and 5 replications. Each treatment was T1: 0.075 ml semen + 0.3 ml of egg yolk sugarcane juice diluent and stored for 24 hours, T2: semen 0.075 ml + 0.3 ml of diluent for egg yolk cane juice and stored for 48 hours, T3: semen 0.075 ml + 0.3 ml of diluent for egg yolk sugarcane juice and stored for 72 hours, T4: semen 0.075 ml + 0 ,3 ml of egg yolk sugarcane juice diluent and stored for 96 hours. The variables measured were individual motility, viability, spermatozoa abnormalities, and semen pH. The results showed that the T4 treatment on a shelf life of 96 hours still showed a good average value with individual spermatozoa motility values of 45%, spermatozoa viability of 77.3%, spermatozoa abnormalities of 12.5% and semen pH 6.6. It can be concluded that the use of cane juice-egg yolk diluent has a positive impact because it is able to maintain the viability of spermatozoa for 96 hours.    

scholarly journals BLOOD CHOLESTEROL, LDL AND HDL IN CROSSBRED LOCAL CHICKEN FEED INULIN OF DAHLIA TUBERS AS A PREBIOTIC

2016 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Nurul Fajrih
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Dietary Treatment ◽  
Blood Cholesterol ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Local Chicken ◽  
Low Cholesterol

The feeding inulin of dahlia tubers as a prebiotic related to the quality of the resulting product is a product low cholesterol. The research was aimed to examine the role of inulin as a prebiotic derived from dahlia flower tuber in the form of powder and extract on blood cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) of crossbred local chicken. The research was assigned in a completely randomized design with 7 treatments and 4 replications (10 birds each), treatments applied were T0: basal ration, T1: ration + 0.4% powder of dahlia tuber, T2: ration + 0.8% powder of dahlia tuber, T3: ration + 1.2% powder of dahlia tuber, T4: ration + 0.39% extract of dahlia tuber, T5: ration + 0.78% extract of dahlia tuber, T6: ration + 1.17% extract of dahlia tuber. The birds were reared for conditioning from day 1 until 3 week, and dietary treatment was given thereafter until 11 week of age. Parameters observed were blood cholesterol, LDL and HDL. The data were statistically analyzed according to ANOVA and continued to Duncan test at the level of 5% probability. The results showed that feeding inulin in the form of powder or extract significantly (P<0,05) decreased LDL and HDL, but not on blood cholesterol. In conclusion, feeding inulin in the form of powder in 1.2% (T3) and extract in 1.17% (T6), able to reduce levels of LDL and HDL but not yet capable of lowering blood cholesterol of crossbred local chicken.

Pengaruh Temperatur dan Lama Thawing terhadap Kualitas Spermatozoa Sapi dalam Penyimpanan Straw Beku

2010 ◽  
Vol 8 (1) ◽  
pp. 22
Author(s):  
S Utomo ◽  
E Boquifai
Keyword(s):  
Key Words ◽  
The Other ◽  
Effect Of Temperature ◽  
Randomized Design ◽  
Frozen Semen ◽  
Completely Randomized Design ◽  
Motility Of Sperm ◽  
Better Than

<p>This research was conducted to investigate the effect of temperature and duration of<br />thawing frozen semen in ministraw on quality sperm. Seventy five ministraws frozen semen of Simmental breed were thawing by water with temperature 5, 26 and 37 °C, with duration of 5, 10, 15, 20 and 30 second. The quality sperm post thawing was measured based on pH and motility. The design of this research was completely randomized design with factorial clasification 3 x 5. The result showed that the motility of sperm was thawing 37°C better than the other temperature. The quality of sperm was no affected by temperature and duration of thawing. It could be concluded<br />that thawing of frozen semen will have better done at 37°C with duration of 15 second.</p><p>Key words : Thawing, sperm, ministraw, frozen semen.</p>

scholarly journals Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Hamdan Hamdan ◽  
Budianto Panjaitan ◽  
Amalia Sutriana ◽  
Dwinna Aliza ◽  
Erdiansyah Rahmi
Keyword(s):  
Experimental Design ◽  
Storage Time ◽  
Sperm Concentration ◽  
Spermatozoa Motility ◽  
Randomized Design ◽  
Sperm Abnormality ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Banda Aceh

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.

Kualitas semen segar kambing kacang pada suhu 50c dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer

2017 ◽  
Vol 5 (2) ◽  
pp. 77-85
Author(s):  
Feri Setiawan ◽  
Enike Dwi Kusumawati
Keyword(s):  
Significant Influence ◽  
Research Method ◽  
Research Result ◽  
Animal Husbandry ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Fresh Semen

Abstrak Penelitian ini bertujuan untuk mengetahui kualitas semen segar Kambing Kacang pejantan pada penyimpanan suhu 5oC dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer. Penelitian ini dilakukan di Laboratorium Fakultas Peternakan Universitas Kanjuruhan Malang pada tanggal 6 sampai 11 Februari 2012. Metode penelitian yang digunakan adalah metode percobaan dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial. Materi penelitian yang digunakan adalah semen segar Kambing Kacang. Berdasarkan hasil penelitian menunjukkan bahwa kualitas spermatozoa Kambing Kacang pada suhu 5oC dengan lama simpan yang berbeda menggunakan pengencer dan tanpa pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0.01). Pada pengamatan motilitas dan viabilitas spermatozoa kambing Kacang pada suhu 5oC baik yang menggunakan pengencer maupun tanpa pengencer menunjukkan adanya perbedaan yang sangat nyata (P<0.01). Tetapi pada pengamatan abnormalitas tidak menunjukkan adanya pengaruh penggunaan pengencer (P>0.01) serta tidak terdapat pengaruh interaksi antara lama simpan dan penggunaan pengencer terhadap abnormalitas spermatozoa kambing Kacang (P>0.01). Namun terdapat pengaruh yang sangat nyata lama simpan terhadap abnormalitas spermatozoa kambing Kacang pada suhu 5oC baik yang menggunakan pengencer maupun tanpa pengencer (P<0.01). Kesimpulan yang diperoleh dari hasil penelitian ini yaitu  bahwa penyimpanan semen segar kambing Kacang pada suhu 50C tanpa menggunakan pengencer mempunyai kualitas yang paling baik yaitu mampu bertahan sampai lama simpan ke 42 jam.   Abstract  This research aimed to determine the quality of a fresh semen Kacang goat at 50C temperature the various preservings time within and withouth extender.  This research had been performed in Animal Husbandry Faculty of  Kanjuruhan University Malang since 6 until 11 February 2012. The research method used was Completely Randomized Design (CRD) Factorial. Research used was fresh semen Kacang goat. The result of the research indicated that spermatozoa quality of Kacang goat at 50C in different preservings time within and without extender showed the significant differentcy (P<0,01). In motility and viability observation spermatozoa of Kacang goat at 50C either with and without extender showed the significant differntcy  (P<0,01). But at the abnormality observation it did not show the influence of the usage extender (P>0,01) and there was no interaction influence between the preserving time and the extender usage forwards the abnormality of Kacang goat spermatozoa (P>0,01). But there was a significant influence of preserving time to wards the abnormality of spermatozoa at 50C temperature either with extender and withoud extender (P<0,01). This research result concludes that the Kacang goat fresh semen preserving without using extender is the best quality that it is able to stay freshly until 42 hours.

scholarly journals The Effect of Age on the Quality of Semen Turkeys (Meleagris gallopavo)

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Riyan Nugroho Aji ◽  
Ismaya Ismaya ◽  
Sri Harimurti ◽  
Heru Sasongko ◽  
Sigit Bintara ◽  
...  
Keyword(s):  
Semen Quality ◽  
Meleagris Gallopavo ◽  
Randomized Design ◽  
Viability Percentage ◽  
Abdominal Massage ◽  
Completely Randomized Design ◽  
Effect Of Age ◽  
Fresh Semen ◽  
Range Test

The purpose of this study was to observe the macroscopic and microscopic quality of Bronze turkey fresh semen on various ages. The observed toms were at the ages of 9 to 10 months (P1), 13 to 14 months (P2), and 17 to 18 months (P3), with each taken from 3 different toms. The collection was done by abdominal massage, and collected for 3 times a week in triplicate. This data was analyzed by analysis of variance (ANOVA) in a completely randomized design, followed by Duncan's New Multiple Range Test (DMRT) if there was any difference. The results showed that the average volume of turkey fresh semen were P1 (0.16 ± 0.04 ml), P2 (0.13 ± 0.02 ml), and P3 (0.10 ± 0.02 ml). The average pH of fresh semen were P1 (7.19 ± 0.05), P2 (7.12 ± 0.10), and P3 (7.06 ± 0.06). The average motility percentage of fresh semen were P1 (78.11 ± 1.38%), P2 (82.55 ± 1.17%), and P3 (74.55 ± 4.16%). The average viability percentage of fresh semen were P1 (78.63 ± 1.55%), P2 (83.26 ± 1.54%), and P3 (79.20 ± 1.06%). The average abnormal percentage of spermatozoa were P1 (3.01 ± 0.04%), P2 (3.04 ± 0.88%), and P3 (3.21 ± 0.44%). In conclusion, the best fresh semen quality is found on toms aged from 13 to14 months (P2). Furthermore, at the age 17 to18 months and more, the semen quality was decreased. 

scholarly journals The Effect of Male Age on the Quality of Bali Cattle Fresh Semen

2017 ◽  
Vol 2 (2) ◽  
pp. 13-18
Author(s):  
N. Nirwana ◽  
S. Suparman
Keyword(s):  
Animal Feed ◽  
Semen Quality ◽  
Age Groups ◽  
Randomized Design ◽  
Semen Volume ◽  
Livestock Feeding ◽  
Bali Cattle ◽  
Observation Method ◽  
Fresh Semen

This research was conducted by conducting experiments in UPTD laboratory of Livestock and Livestock Feeding Agricultural Service of Sulawesi Barat Province. The purpose of this research is to compare the age of males during March to April 2013. The results of this research are expected to be used as a guideline for the use of Bali Cattle research as a semen producer based on the age of livestock that can be used as one of the criteria in the selection of prospective semen producing males. The research material is fresh semen of Bali Cattle with requirement of 70% individual motility. The research method used is observation method by observing the fresh semen quality of Bali Cattle research which has been classified into 3 groups based on age 3, 5, and 6 years. The data analysis used is the analysis of the variety and the design is Completely Randomized Design. The results showed that the quality of fresh semen in the three age groups (3, 5, and 6 years) where volume: 5.2 ± 1.2; 6.4 ± 1.1; And 6.4 ± 0.8 ml, color: milk, white, pH: 6,4 ± 0,1; 6.4 ± 0.1; And 6.3 ± 0.1, consistency: concentrated; Medium; Concentrated and concentrated, concentrations: 1909.6 ± 418.8; 1223.3 ± 220.8; And 1790.8 ± 240.5 million / ml, and 92.5 ± 2.9%, abnormality: 4.4 ± 1.2; 4.3 ± 1.2; And 3.3 ± 1.0%. The conclusions of this research indicate that the age of males gives a significant effect on the fresh semen volume, fresh semen concentration, gives a very significant effect on fresh semen concentration and does not have a significant effect on fresh semen sperm abnormalities. And a male of Bali Cattle age 3-6 years old in UPTD Livestock Feeding and Animal Feed Agricultural Service of Sulawesi Province has good semen quality for Artificial Insemination.

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