scholarly journals Physiological and biochemical responses of barley to application of bio-fertilizers and nano iron oxide under salinity stress in greenhouse

2018 ◽  
Vol 47 (4) ◽  
pp. 863-875
Author(s):  
Siyamak Dadashzadeh ◽  
Raouf Seyed Shariff ◽  
Salim Farzaneh

To study the effects of bio-fertilizers and nano iron oxide on some physiological and biochemical traits of barley under salinity stress, a factorial experiment was conducted based on RCBD with three replications under greenhouse condition. Factors in the study, included four levels of salinity, four levels of nano iron oxide use and four preparations of bio-fertilizer application. Increasing salinity in the soil decreased chlorophyll content, quantum yield, relative water content and grain yield, whereas soluble sugars, proline content, electrical conductivity and the activities of catalase, peroxidase and polyphenol oxidase enzymes increased. Inoculation of plants with bio-fertilizers and nano iron oxide application improved these traits (except electrical conductivity) under salinity as well as normal conditions. Results showed that application of Azospirillum and mycorrhiza and 0.9 g/l nano iron oxide (B3Fe3) increased about 15.45% from grain yield in comparison with B4Fe4 under the highest salinity level. Based on the results, it seems that bio-fertilizers and nano iron oxide application can be useful in alleviating salinity stress in barley.

2016 ◽  
Vol 67 (11) ◽  
pp. 1158 ◽  
Author(s):  
Rupinder Kaur ◽  
Seema Bedi ◽  
Gulshan Mahajan ◽  
Gurpreet Kaur ◽  
Bhagirath Singh Chauhan

To achieve high productivity of labour and water in rice cropping, farmers in South Asia have recently shown more interest in dry direct-seeded rice (DSR). An understanding of physiological and biochemical traits associated with high grain yield and efficiency of nitrogen (N) use is important to the development of genotypes for DSR. We investigated this issue with rice genotypes adapted to DSR in response to N rates. A 2-year study was conducted in a factorial randomised complete block design with eight genotypes and two N rates (75 and 150 kg N ha–1). Almost all of the physiological and biochemical traits studied (e.g. plant height, chlorophyll content, panicle weight, soluble sugars, starch) in DSR improved with increasing N from 75 to 150 kg ha–1, resulting in a 6% increase in yield at 150 kg N ha–1 relative to 75 kg N ha–1. Partial factor productivity of N was highest for the genotype IET-23455 (72.4 kg kg–1) and lowest for the genotype AAUDR (37.4 kg kg–1). Our results suggest that genotypes such as IET-23455 can maintain grain yield at low N rates as N-efficient genotypes. The greater biochemical activity (nitrate reductase and glutamine synthetase, sugar, protein and proline) and higher photosynthetic N-use efficiency at low N rates could be used in selection for N-efficient rice genotypes for DSR.


2018 ◽  
Vol 16 (3) ◽  
pp. 357-365
Author(s):  
Syeda Ariana Ferdous ◽  
Mohammad Noor Hossain Miah ◽  
Mozammel Hoque ◽  
Sazzad Hossain ◽  
Ahmed Khairul Hasan

The effect of lime and fertilizer application, as the management of soil acidity, on the growth and yield of rice cv. BRRI dhan50 was investigated during Aman rice season at the Agronomy Field Laboratory of Sylhet Agricultural University, Bangladesh. The experiment was consisted of two factors namely lime and fertilizer. There were four levels of lime (0, 0.50, 1.00, and 1.50 t ha–1 of CaCO3.MgCO3) and three levels of fertilizers (control, FYM @ 10 t ha–1, and chemical fertilizer @ 100-30-42-4-3-0.4 kg ha–1 of N-P-K-Ca-S-Zn). The experiment was laid out in a randomized complete block design with three replications where the unit plot size was 4.0 m x 2.5 m. Growth parameters, yield components and yield of BRRIdhan 50 rice increased with increasing lime rate in association of fertilizer in acidic soil. The highest grain yield (2.90 t ha–1) was recorded from the application of 1.50 t ha–1 lime and the lowest (2.06 t ha–1) was from control (0t ha–1), irrespective of fertilizer. On the other hand, the best effect of fertilizers on grain yield (3.08 t ha–1) was found with the application of FYM @ 10 t ha–1 and the lowest yield (1.59 t ha–1) was in control. The treatment combination of lime 1.50 t ha–1 and FYM (@ 10 t ha–1 produced the highest grain yield (3.60 t ha–1), which was followed by treatment combination of lime 1.50 t ha–1 and chemical fertilizer @ 100-30-42-4-3-0.4 kg ha–1 of N-P-K-Ca-S-Zn (3.28 t ha–1). Additionally, application of lime and FYM improved the soil fertility and properties of acidic soil for crop production by increasing the pH, organic matter and availability of some essential nutrients. From the study, it was indicated that both FYM and lime could affect to enhance the grain yield of rice in acidic soil. J. Bangladesh Agril. Univ. 16(3): 357–365, December 2018


2019 ◽  
Author(s):  
Asunta Mukami ◽  
Alex Ng’etich ◽  
Easter Syombua ◽  
Richard Oduor ◽  
Wilton Mbinda

AbstractFinger millet is one of the most important cereals that are often grown in semiarid and arid regions of East-Africa. Salinity is known to be a major impediment for the crop growth and production. This study was aimed to understand the mechanisms of physiological and biochemical responses to salinity stress of Kenyan finger millet varieties (GBK043137, GBK043128, GBK043124, GBK043122, GBK043094, GBK043050) grown across different agroecological zones under NaCl-induced salinity stress. Seeds were germinated on the sterile soil and treated using various concentrations of NaCl (100, 200 and 300 mM) for two weeks. Again, the early-seedling stage of germinated plants was irrigated with the same salt concentrations for 60 days. Results indicated depression in germination percentage, shoot and root growth rate, leaf relative water content, chlorophyll content contents, leaf K+ concentration, and leaf K+/Na+ ratios increased salt levels. Contrary, proline and malonaldehyde (MDA) contents reduced sugar content and leaf total proteins. At the same time, the leaf Na+ and Cl− amounts of all plants increased substantially with rising stress levels. Clustering analysis revealed that GBK043094 and GBK043137 were placed together and identified as salt-tolerant varieties based on their performance under salt stress. Overall, our findings indicated a significant varietal variability for most of the parameters analysed. These superior varieties identified could be potentially used as promising genetic resources in future breeding programmes development directed towards salt-tolerant finger millet hybrids. Further analysis at genomic level need to be undertaken to better understand the genetic factors that promote salinity tolerance in finger millet.


2019 ◽  
Vol 9 (4) ◽  
pp. 535-541
Author(s):  
T. Fatiha ◽  
H. Abdelkrim ◽  
K. Mostefa ◽  
R. Waffa

The victory over the protein deficiency afflicting millions of human beings is a crucial and urgent task for world agriculture. The legume Lentil (Lens culinaris Medik) is one of the largest sources of protein with an average of 25% of all seeds produced worldwide. When used in crop rotation, it fertilizes the soil, as is the case in North Africa and this plant holds an important place throughout the world. In Algeria, lentil is largely growth in the semi-arid zones of the interior plains characterized by various abiotic constraints such as terminal drought which causes significant losses in lentil yield every year. For this purpose, the cases in this study aims to evaluate the effect of end of cycle water stress on the behavior of four varieties of lentil (Syrie 229, Metropole, Balkan 75 and Ibela) and to elucidate the plant’s morpho-physiological and biochemical parameters involved in tolerance and to assess varieties which could be grown under water stress conditions. The experiment was conducted in a laboratory and greenhouse with a well-controlled condition. The experiment was arranged in a completely randomized design of two factors (water stress and varieties) with four replications and two treatments. Not treated plants (without water stress application) were utilized as control. The four tested genotypes were subjected to two water regimes, one irrigated throughout the cycle (No stressed) used as a control, the other one stressful from the beginning of flowering till seed’s filling stages (stressed). The study covered the variation of the relative water content in leaves as well as the leaf surface, the rate of proline and soluble sugars of the leaves and the total seed protein content. The obtained results showed that the studied genotypes behaved differently to the water stress. Thus, a significant decrease was observed in the relative water content from 75.29% to 70.71% and in the leaf area from 16.76 cm2 to 13.63 cm2. An opposite behavior was observed on the osmoticum accumulated as a response to water deficit. A significant increase in proline (150.14 μg/g DM to 203.69 μg/g DM) and in soluble sugars stress (60.42 μg/g DM to 110.21 μg/g DM) was detected in leaves under stressed conditions. The terminal water stress resulted also in an increase in protein content in lentil seeds from a mean value of 22.08% to 24.84%. Regarding the obtained results, Metropole genotype seems to be the most tolerant cultivar to water stress followed by Balkan 755.


2021 ◽  
Author(s):  
Alireza Pour-Aboghadareh ◽  
Mohammad Reza Mehrvar ◽  
Sara Sanjani ◽  
Ashkboos Amini ◽  
Hamidreza Nikkhah Chaman-Abad ◽  
...  

Abstract The salinity tolerance of 17 breeding wheat genotypes along with three local varieties was evaluated under control and salinity stress (160 mM NaCl) conditions. At the seedling stage, shoot and root dry weights, relative water content (RWC), membrane stability index (MSI), relative chlorophyll content (SPAD index), root and shoot Na+ (RN and SN), root and shoot K+ (RK and SK), root and shoot K+/Na+ ratios (RKN and SKN), root-to-shoot Na+ translocation (RTSN), root-to-shoot K+ translocation (RTSK), stomatal conductance (GS), transpiration rate (TE), and photosynthesis rate (PN) were measured. Moreover, the investigated genotypes were assessed in terms of grain yield across four saline regions during the 2018–2019 cropping seasons. Salinity stress caused a significant reduction in the RDW, SDW, PN, GS, TE, SK, RKN, SKN, RTSN, and RTSK, but resulted in increased RN, RK, and SN. The results of AMMI analysis of variance also indicated significant differences among test locations, genotypes, and their interaction effects. The PCA-based biplot revealed that grain yield strongly correlated with RKN and RK. Furthermore, the correlation among PN, GS, and TE traits was strong and positive and had a positive correlation with RWC, MSI, RDW, and SPAD index. Considering our results, RK and RKN were identified as useful physiological tools to screen salt tolerance at the early-growth stage. According to the ranking patterns obtained by the average sum of ranks method (ASR) and grain yield, we observed that genotype number G5 had considerable physiological potential at the early-growth stage and also responded well to soil salinity at the farm; thus this genotype can be promoted for commercial production.


2014 ◽  
Vol 12 (1) ◽  
pp. 55-60 ◽  
Author(s):  
MS Islam ◽  
SK Paul ◽  
MAR Sarkar

An experiment was carried out at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during June to December 2012 with a view to finding out the effect of variety and nitrogen fertilizer application on the growth and yield of transplant Aman rice. The experiment comprised four varieties viz. BRRI dhan33, BRRI dhan34, BRRI dhan39 and BRRI dhan46, and four levels of nitrogen viz. control (no urea), prilled urea (50 kg N ha-1), one pellet (0.9g) of USG/4 hills of two adjacent rows (30 kg N ha-1) was applied at 10 DAT and two pellets of USG (0.9g each) one applied at 10 DAT and the other at 45 DAT/4 hills of two adjacent rows (60 kg N ha-1). The experiment was laid out in a randomized complete block design with three replications. Results of the experiment showed that variety and level of nitrogen application had significant effect on yield attributes and yield of transplant Aman rice. At maturity the tallest plant (122.60 cm) and the highest number total tillers hill-1 (12.72) were observed in two pellets of USG/4 hills providing 60 kg N ha-1. The highest number of tillers hill-1 (10.08) was obtained from BRRI dhan34 and the lowest one (9.88) from BRRI dhan33. The highest grain yield was obtained from BRRI dhan46 (4.56 t ha-1) and the lowest one (3.27 t ha-1) was obtained from BRRI dhan34. The interaction reveals that the highest grain yield (5.61 t ha-1) was obtained from the treatment combination of BRRI dhan46 with two pellets of USG (0.9g each) applied one at 10 DAT and the other at 45 DAT.DOI: http://dx.doi.org/10.3329/jbau.v12i1.21239 J. Bangladesh Agril. Univ. 12(1): 55-60, June 2014


2017 ◽  
Vol 31 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Mahnaz Khatar ◽  
Mohammad Hossein Mohammadi ◽  
Farid Shekari

AbstractThe effect of soil matric suction (2-33 kPa) and salinity (soil solution electrical conductivity 0.7-8 dS m−1for bean and 2-20 dS m−1for wheat) on some physiological characteristics of bean and wheat in a clay loam soil under greenhouse condition was investigated. The results showed that the leaf chlorophyll content index and potassium concentration decrease under salinity stress and increase with matric suction from 2 to 33 kPa suction for both plants. The wheat chlorophyll content index declines during the stress spell but bean chlorophyll content index remains nearly constant. The lowest values of the content of soluble sugars and the highest values of leaf proline content are observed at2 kPa matric suction (highest aeration stress) for bean and wheat. As matric suction increases from 2 to 6 kPa, the soluble sugars increases and proline content decreases significantly and then soluble sugars decreases and proline content increases until 10 kPa suction, and the soluble sugars remains nearly constant at the higher matric suctions for both plants. While the electrical conductivity effect on the soluble sugars is not significant, the values of proline content for both crop increase significantly with electrical conductivity. It was shown that the aeration stress can result in more considerable and rapid physiological responses, in comparison with salinity stress. There is a strong correlation between wheat and bean chlorophyll content index and potassium concentration under salinity and aeration stresses.


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 541
Author(s):  
Victoria Otie ◽  
Idorenyin Udo ◽  
Yang Shao ◽  
Michael O. Itam ◽  
Hideki Okamoto ◽  
...  

Salinity episodes that are common in arid regions, characterized by dryland, are adversely affecting crop production worldwide. This study evaluated the effectiveness of brassinolide (BL) in ameliorating salinity stress imposed on soybean at four levels (control (1.10), 32.40, 60.60 and 86.30 mM/L NaCl) in factorial combination with six BL application frequency (control (BL0), application at seedling (BL1), flowering (BL2), podding (BL3), seedling + flowering (BL4) and seedling + flowering + podding (BL5)) stages. Plant growth attributes, seed yield, and N, P, K, Ca and Mg partitioning to leaves, stems and roots, as well as protein and seed-N concentrations, were significantly (p ≤ 0.05) reduced by salinity stress. These trends were ascribed to considerable impairments in the photosynthetic pigments, photosynthetically active radiation, leaf stomatal conductance and relative water content in the leaves of seedlings under stress. The activity of peroxidase and superoxidase significantly (p ≤ 0.05) increased with salinity. Foliar spray with BL significantly (p ≤ 0.05) improved the photosynthetic attributes, as well as nutrient partitioning, under stress, and alleviated ion toxicity by maintaining a favourable K+/Na+ ratio and decreasing oxidative damage. Foliar spray with brassinolide could sustain soybean growth and seed yield at salt concentrations up to 60.60 mM/L NaCl.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1349
Author(s):  
Ahlam Khalofah ◽  
Mona Kilany ◽  
Hussein Migdadi

Heavy metals are primarily generated and deposited in the environment, causing phytotoxicity. This work evaluated fenugreek plants’ morpho-physiological and biochemical responses under mercury stress conditions toward Ag nanoparticles and Sphingobacterium ginsenosidiumtans applications. The fabrication of Ag nanoparticles by Thymus vulgaris was monitored and described by UV/Vis analysis, FTIR, and SEM. The effect of mercury on vegetative growth was determined by measuring the root and shoots length, the number and area of leaves, the relative water content, and the weight of the green and dried plants; appraisal of photosynthetic pigments, proline, hydrogen peroxide, and total phenols content were also performed. In addition, the manipulation of Ag nanoparticles, S. ginsenosidiumtans, and their combination were tested for mercury stress. Here, Ag nanoparticles were formed at 420 nm with a uniform cuboid form and size of 85 nm. Interestingly, the gradual suppression of vegetal growth and photosynthetic pigments by mercury, Ag nanoparticles, and S. ginsenosidiumtans were detected; however, carotenoids and anthocyanins were significantly increased. In addition, proline, hydrogen peroxide, and total phenols content were significantly increased because mercury and S. ginsenosidiumtans enhance this increase. Ag nanoparticles achieve higher levels by the combination. Thus, S. ginsenosidiumtans and Ag nanoparticles could have the plausible ability to relieve and combat mercury’s dangerous effects in fenugreek.


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