scholarly journals Análise microbiológica da água utilizada para consumo nas escolas de Esperança, Paraíba

2017 ◽  
Vol 1 (37) ◽  
pp. 11 ◽  
Author(s):  
Aldeni Barbosa Da Silva ◽  
Janaina Moreira De Brito ◽  
Jonatas Da Silva Duarte ◽  
Olga Elyzabeth Lucena Almeida
Keyword(s):  
Public Schools ◽  
Microbiological Quality ◽  
Human Consumption ◽  
State University ◽  
Total Coliforms ◽  
E Coli ◽  
Wide Mouth ◽  
Yellow Coloration ◽  
C 30

This project had the objective of verifying the microbiological quality of the water that supplies seven public schools in the urban area of the municipality of Esperança-PB. Initially, the samples were collected in wide - mouth glass bottles, protected with laminated paper, pre-sterilized (121º C - 30’ in autoclave), and were sent to the Environmental Sanitation Laboratory of the State University of Paraíba - Campina Grande Campus, where the qualitative analysis of Total Coliforms and Escherichia coli were performed using the Colilert Enzymatic Chromogenic Substrate method. Soon after the application of the Colilert reagent and incubation for 24 hours at 37 ° C, a yellow coloration was observed in samples 6 and 7, highlighting the presence of total coliforms. However, the presence of E. coli was not detected in any of the analyzed samples, since they did not show fluorescence under ultraviolet light. This concludes that the waters of samples 6 and 7 are inappropriate for human consumption. According to Ordinance No. 2.914 / 2011 of the Ministry of Health, when samples with positive results are detected for total coliforms, even in presumptive tests, corrective actions must be taken and new samples must be collected on successive days until satisfactory results are obtained.

scholarly journals Are the defined substrate-based methods adequate to determine the microbiological quality of natural recreational waters?

2009 ◽  
Vol 8 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Marta Sofia Valente ◽  
Paulo Pedro ◽  
M. Carmen Alonso ◽  
Juan J. Borrego ◽  
Lídia Dionísio
Keyword(s):  
Standard Method ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
Recreational Waters ◽  
Total Coliforms ◽  
E Coli ◽  
Quality Of Water ◽  
Routine Monitoring ◽  
Rapid Tests

Monitoring the microbiological quality of water used for recreational activities is very important to human public health. Although the sanitary quality of recreational marine waters could be evaluated by standard methods, they are time-consuming and need confirmation. For these reasons, faster and more sensitive methods, such as the defined substrate-based technology, have been developed. In the present work, we have compared the standard method of membrane filtration using Tergitol-TTC agar for total coliforms and Escherichia coli, and Slanetz and Bartley agar for enterococci, and the IDEXX defined substrate technology for these faecal pollution indicators to determine the microbiological quality of natural recreational waters. ISO 17994:2004 standard was used to compare these methods. The IDEXX for total coliforms and E. coli, Colilert®, showed higher values than those obtained by the standard method. Enterolert® test, for the enumeration of enterococci, showed lower values when compared with the standard method. It may be concluded that more studies to evaluate the precision and accuracy of the rapid tests are required in order to apply them for routine monitoring of marine and freshwater recreational bathing areas. The main advantages of these methods are that they are more specific, feasible and simpler than the standard methodology.

scholarly journals Microbiological Quality of Kachumbari, a Raw Vegetable Salad Popularly Served Alongside Roast Meat in Kenya

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Kenneth M. Mbae ◽  
Mercy K. Ndwiga ◽  
Fredrick G. Kiruki
Keyword(s):  
Cross Section ◽  
Microbiological Quality ◽  
Disease Outbreaks ◽  
Human Consumption ◽  
Microbial Quality ◽  
Protection Agency ◽  
Indicator Microorganisms ◽  
E Coli ◽  
Food Borne

Raw salads are regularly implicated in food-borne disease outbreaks globally. Consumption of kachumbari, a raw vegetable salad, alongside roast meat is widespread in Kenya. The aim of this study was to evaluate the bacteriological quality of kachumbari samples (n=39) collected from a cross section of roasted meat eateries in Kenya. The United Kingdom’s Health Protection Agency guidelines were used to infer safety of the salads due to lack of local criteria for microbiological safety of ready-to-eat fresh produce placed in the market. Based on Escherichia coli counts, 14 (35.9%) of the samples were of satisfactory microbial quality (<20 CFU/g), 7 (17.9%) in the borderline (20–≤102 CFU/g), and 18 (46.2%) unsatisfactory (>102 CFU/g). All samples examined for staphylococci had counts falling within the borderline range (20–≤104 CFU/g). Collectively, 3 (7.7%) of the sampled salads were classified as potentially harmful to health and/or unfit for human consumption due to the presumptive presence of 2 (5.1%) Campylobacter spp. and 1 (2.6%) E. coli O157. Salmonella was not detected in any of the samples. The presence of hygiene indicator microorganisms and pathogens demonstrates that kachumbari salads present a public health risk.

scholarly journals Microbiological quality of shellfish and evaluation of compact dry EC for detecting total coliforms and Escherichia coli

2020 ◽  
Vol 49 (1) ◽  
pp. 32-39 ◽  
Author(s):  
A. Vásquez García ◽  
S.H. Gomes de Sá ◽  
G. de Sousa Silva ◽  
J.E. Mejia Ballesteros ◽  
E. Barbieri ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Conventional Method ◽  
Microbiological Quality ◽  
Correlation Coefficients ◽  
Mean Values ◽  
Total Coliforms ◽  
E Coli ◽  
The Mean

The objective of this study was to evaluate the microbiological quality of oysters and mussels grown in Cananéia, Brazil, by analysing mesophiles, psychrothophic bacteria, moulds and yeasts, Staphylococcus aureus, and Salmonella spp., and to compare the efficiency of Compact Dry EC method and the conventional method for counting of total coliforms and Escherichia coli. The microbial analysis showed that the mean values of mesophilic counts were 3.14±0.81 log CFU g−1 for oysters and 3.92±0.90 for mussels; the mean values of psychrophilic counts were 2.78±0.75 log CFU g−1 for oysters and 3.22±0.75 log CFU g−1 for mussels; the mean values of mould and yeast counts were 3.70±0.58 log CFU g−1 for oysters and 3.33±0.81 log CFU g−1 for mussels. Salmonella spp. did not present positive results, and the maximal count of Staphylococcus aureus was 1.7 log CFU g−1, therefore, within the limits established in the legislation. The correlation coefficients between the Compact Dry EC method and conventional method were >0.87 for total coliform and E. coli counts for both types of shellfish. The data in this study show that the Compact Dry EC method is an acceptable alternative to conventional methods for enumeration of total coliforms and E. coli in shellfish.

scholarly journals Indicator of Quality of Water for Human Consumption in the Community El Comején, Masaya (Nicaragua)

2019 ◽  
Vol 11 (8) ◽  
pp. 176
Author(s):  
Dixon Nohel Morales López ◽  
Ismael Montero Fernández ◽  
Selvin Antonio Saravia Maldonado ◽  
Francisco Luis Acosta Díaz ◽  
Luis Antonio Beltrán Alemán
Keyword(s):  
Major Element ◽  
Poor Quality ◽  
Human Consumption ◽  
Total Coliforms ◽  
E Coli ◽  
The Poor ◽  
Quality Of Water ◽  
Sampling Points ◽  
The Republic

Knowing the quality of water for human consumption is of utmost importance in the development of a country, since the poor quality of this vital liquid can be a source of diseases for the health of the population. The present work was carried out in the community El Comej&eacute;n, Masaya, in the Republic of Nicaragua, where microbiological analyzes were carried out in three sampling points (one well drilled and two taps). The physicochemical and chemical analyzes are in accordance with the norms established by CARPE, highlighting sodium as a major element with a concentration of 48.6 mg L-1 and potassium with 21.08 mg L-1. On the other hand, biological parameters such as total coliforms, thermotolerable coliforms, E. coli and fecal enterococos were analyzed, being found in some sampling points, values of total coliforms of 110 NMP 100 mL-1 being above the established limits, requiring a treatment additional to be suitable for human consumption.

scholarly journals The effect of container-biofilm on the microbiological quality of water used from plastic household containers

2003 ◽  
Vol 1 (3) ◽  
pp. 101-108 ◽  
Author(s):  
P. Jagals ◽  
C. Jagals ◽  
T. C. Bokako
Keyword(s):  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Human Consumption ◽  
Microbiological Contamination ◽  
Supply Source ◽  
E Coli ◽  
Quality Of Water ◽  
Storage Containers

Studies in Southern Africa have shown that even when microbiologically safe water is supplied to developing communities at communal standpipes, contamination by high numbers of pathogenic microorganisms may occur during the processes of fetching water from the supply source and storage during use at home, rendering such waters unsafe for human consumption. This study investigated the occurrence of biofilm in PVC storage containers as one possible reason for this deterioration, using heterotrophic bacteria and total coliform counts as well as turbidity as indicators. A second objective was to determine whether biofilm in water-storage containers could contribute to hazardous microbiological contamination indicated by Escherichia coli and Clostridium perfringens. Results indicated that increased microbiological contamination is associated with biofilm. The biofilm harbours heterotrophic bacteria, total coliforms and C. perfringens. E. coli could not be associated directly with the levels of biofilm in containers but rather appears to be introduced intermittently from the ambient domestic environment. When dislodged with the biofilm, these bacteria contributed substantially to the deterioration of the microbiological quality of supplied water stored in plastic containers.

Assessment of microbiological quality of drinking water from household tanks in Bermuda

2008 ◽  
Vol 54 (6) ◽  
pp. 495-500 ◽  
Author(s):  
B. Lévesque ◽  
D. Pereg ◽  
E. Watkinson ◽  
J.S. Maguire ◽  
L. Bissonnette ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Membrane Filtration ◽  
Microbiological Quality ◽  
General Information ◽  
Total Coliforms ◽  
E Coli ◽  
Electoral Register ◽  
Highly Correlated

Bermuda residents collect rainwater from rooftops to fulfil their freshwater needs. The objective of this study was to assess the microbiological quality of drinking water in household tanks throughout Bermuda. The tanks surveyed were selected randomly from the electoral register. Governmental officers visited the selected household (n = 102) to collect water samples and administer a short questionnaire about the tank characteristics, the residents’ habits in terms of water use, and general information on the water collecting system and its maintenance. At the same time, water samples were collected for analysis and total coliforms and Escherichia coli were determined by 2 methods (membrane filtration and culture on chromogenic media, Colilert kit). Results from the 2 methods were highly correlated and showed that approximately 90% of the samples analysed were contaminated with total coliforms in concentrations exceeding 10 CFU/100 mL, and approximately 66% of samples showed contamination with E. coli. Tank cleaning in the year prior to sampling seems to protect against water contamination. If rainwater collection from roofs is the most efficient mean for providing freshwater to Bermudians, it must not be considered a source of high quality drinking water because of the high levels of microbial contamination.

scholarly journals Assessment of Microbiological Quality of Fresh-cut, Processed and Preserved Mushrooms Available in and Around Dhaka City

1970 ◽  
Vol 27 (2) ◽  
pp. 42-45
Author(s):  
AS Mustafa Kamal ◽  
Fauzia Begum ◽  
Abul Khair
Keyword(s):  
Fecal Coliform ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Food Products ◽  
Point Of View ◽  
Human Consumption ◽  
Dhaka City ◽  
Canned Food ◽  
E Coli

The results revealed that 40% of fresh mushrooms, 93% of processed and preserved mushroom samples including mushroom-based food products were safe for human consumption from bacteriological point of view. The highest bacterial load recorded was 8.7 × 108 cfu / gm in the samples collected from Sutrapur area of Dhaka city. The results also indicated that 27, 13, 13 and 7% of the fresh mushrooms were contaminated with coliform, fecal coliform, E. coli and Salmonella sp. respectively. Thus those mushroom samples were not up to the mark for consumption, considering safety and sanitation point of view. In case of processed mushrooms and mushroom-based food products the results showed that 20% of dried mushrooms were contaminated with both coliform and fecal coliform, 7% of powdered mushrooms were contaminated with only coliform and 13% of mushroom soup powder were contaminated with coliform, E. coli and Salmonella sp. In case of preserved mushrooms, it was found that 7% of the canned mushrooms had count <10 cfu/gm which was unacceptable as per specifications for the canned food. However, the results also revealed that coliform, fecal coliform, E. coli and even Salmonella sp. were not detected in preserved mushrooms examined.DOI: http://dx.doi.org/10.3329/bjm.v27i2.9170 BJM 2010; 27(2): 42-45

Microbiological Quality and Prevalence of β-Lactam Antibiotic Resistance Genes in Oysters (Crassostrea rhizophorae)

2017 ◽  
Vol 80 (3) ◽  
pp. 488-496 ◽  
Author(s):  
Maria Aparecida da RessurreiÇão Brandão ◽  
Amanda Teixeira Sampaio Lopes ◽  
Maria Tereza da Silva Neta ◽  
Rhyan Barros Farias de Oliveira ◽  
Rachel Passos Rezende ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Antibiotic Resistance Genes ◽  
Human Consumption ◽  
E Coli ◽  
Lactam Antibiotic ◽  
Crassostrea Rhizophorae

ABSTRACTThe microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. The objective of this study was to assess the bacterial composition of the edible oyster Crassostrea rhizophorae in urban and preserved estuaries. Particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blaTEM, blaSHV, and blaKPC) via quantitative PCR of oyster DNA. Our results detected total coliforms, Escherichia coli, and enterobacteria in the oysters from urban estuaries, which is indicative of poor water quality. In addition, our detection of the eaeA and stxA2 virulence genes in 16.7% of E. coli isolates from oysters from this region suggests the presence of multiantibiotic-resistant enteropathogenic and enterohemorrhagic E. coli strains. During periods of low precipitation, increased contamination by E. coli (in winter) and Vibrio parahaemolyticus (in autumn) was observed. In contrast, cultivated oysters inhabiting monitored farms in preserved areas had low levels of bacterial contamination, emphasizing that oyster culture monitoring enhances food quality and makes oysters fit for human consumption. Distinct antibiotic resistance profiles were observed in bacteria isolated from oysters collected from different areas, including resistance to β-lactam antibiotics. The presence of the blaTEM gene in 91.3% of oyster samples indicated that microorganisms in estuarine water conferred the capability to produce β-lactamase. To our knowledge, this is the first study to directly quantify and detect β-lactam antibiotic resistance genes in oysters. We believe our study provides baseline data for bacterial dynamics in estuarine oysters; such knowledge contributes to developing risk assessments to determine the associated hazards and consequences of consuming oysters from aquatic environments containing pathogenic bacteria that may possess antibiotic resistance genes.

scholarly journals Microbial contamination in industrial tofu

2017 ◽  
Vol 47 (3) ◽  
Author(s):  
Thaís Teresa Brandão Cavalheiro Ribeiro ◽  
Géssica Costa ◽  
Marisa da Costa
Keyword(s):  
Bacillus Cereus ◽  
Microbial Contamination ◽  
Microbiological Quality ◽  
Human Consumption ◽  
Microbial Quality ◽  
Porto Alegre ◽  
E Coli ◽  
Food Borne ◽  
Food Borne Illness

ABSTRACT: This study aimed to evaluate the microbiological quality of tofu sold in supermarkets in Porto Alegre/Brazil. Bacteria counts were performed for Bacillus cereus , mesophilic, coliforms and Staphylococcus coagulase positive and negative. The presence of Listeria sp. was also evaluated. Two different brands of tofu (A and B) were collected, one lot per month, for six months. Five samples from each lot were analyzed. All lots presented mesophilic aerobic counts above 4.3x105CFU g-1. Four of the six lots from brand A and all lots from brand B showed E. coli and/or Staphylococcus coagulase positive counts above the Brazilian law accepted limits. The Staphylococcus coagulase negative counts were higher than those of coagulase positive in all lots. In all lots where Staphylococcus coagulase positive counts were above the legal limit, there were counts of coagulase negative above 104CFU g-1. B. cereus and Listeria sp. were not found in either brand. The majority of lots of brand A and all lots of brand B were unsuitable for human consumption. Our results showed that there are problems in tofu manufacturing in both industries analyzed. There is a need of improvement on its microbial quality to avoid problems of food-borne illness, and finally the need of a better control by the Brazilian inspection services.

Mineral water: a microbiological approach

2012 ◽  
Vol 12 (5) ◽  
pp. 556-562 ◽  
Author(s):  
Maria Fernanda Falcone-Dias ◽  
Guilherme L. Emerick ◽  
Adalberto Farache-Filho
Keyword(s):  
Mineral Water ◽  
Microbiological Quality ◽  
Public Health Problem ◽  
Quality Standard ◽  
Plate Count ◽  
Important Public Health Problem ◽  
Total Coliforms ◽  
E Coli ◽  
Bottled Mineral Water

The microbiological quality of bottled mineral water of various domestic brands sold in Brazil was investigated, with particular focus on the heterotrophic plate count (HPC). Neither total coliforms nor Escherichia coli were found in any 1.5 L bottle samples. Total coliforms were found in 2.9% of the small bottles, while in 20 L bottles the presence of total coliforms and E. coli was demonstrated in 15.5 and 2.4% of samples, respectively. Pseudomonas aeruginosa was detected in 4.3, 4.5 and 9.5% of small, 1.5 and 20 L bottles, respectively. In 36.4% of the samples of 1.5 L bottles, the HPC was above 500 cfu/mL. This percentage of samples with an HPC above 500 cfu/mL increased to 52.0 and 61.9% in small and 20 L bottles, respectively. Higher contamination by total coliforms, E. coli, P. aeruginosa and HPCs occurred in 20 L bottles. In conclusion, several samples in this study were outside the international quality standard for mineral water and the large number of samples with high HPCs shows that more work must be done on the use of HPC in mineral water and the damaging effects that these microorganisms may cause to humans. The bottled mineral water was confirmed as a particularly important public health problem, due to the poor microbiological quality of the products that are marketed.

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