scholarly journals Antimicrobial activity of n-hexane and Ethyl acetate extracts of Erythrina stricta Roxb

1970 ◽  
Vol 27 (2) ◽  
pp. 65-66
Author(s):  
Mohammad Musarraf Hussain ◽  
M Mizanur Rahman Mughal ◽  
Md Masud Alam ◽  
Mohammad Golam Dastagir ◽  
AHM Masum Billah ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Key Words ◽  
Microbial Growth ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Zone Of Inhibition ◽  
Microbiological Investigation ◽  
Ethyl Acetate Extracts

The crude n-hexane and ethyl acetate extract of the stem bark of E. stricta were subjected to microbiological investigation and were found to be significantly inhibitory to microbial growth, with the average zone of inhibition 12–17 and 10–16 mm, respectively. In the cytotoxic observation, the n-hexane and ethyl acetate extracts were found to show LC50 of 2.1 and 0.316 mg/ml respectively. Key words: Erythrina stricta; Fabaceae; Antimicrobial activities; Cytotoxicity observation. DOI: http://dx.doi.org/10.3329/bjm.v27i2.9176 BJM 2010; 27(2): 65-66

Steriod and Triterpenoid from Anogeissus latifolia

1970 ◽  
Vol 6 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Mohammad S Rahman ◽  
Mohammad Z Rahman ◽  
ABM Ahad Uddin ◽  
Mohammad A Rashid
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Inhibitory Activity ◽  
Full Text ◽  
Brine Shrimp ◽  
Microbial Growth ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Disc Diffusion ◽  
Antimicrobial Screening

3-?-hydroxy-28-acetyltaraxaren (1) and ?-sitosterol (2) were isolated from an ethyl acetate extract of the stem bark of Anogeissus latifolia. The ethyl acetate and methanol extracts when subjected to antimicrobial screening showed significant inhibitory activity to microbial growth, while the ethyl acetate showed demonstrated significant cytotoxicity to brine shrimp with LC50 of 0.50 ?g/ml. Key words: Anogeissus latifolia, Combretaceae, 3-?-hydroxy-28-acetyltaraxaren, ?-sitosterol, antimicrobial, cytotoxiciy, disc diffusion Dhaka Univ. J. Pharm. Sci. 6(1): 47-50, 2007 (June) The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Antimicrobial Activity of Allium chinense G. Don.

2017 ◽  
Vol 2 (3) ◽  
pp. 129-138
Author(s):  
Frans Grovy Naibaho ◽  
Maria Bintang ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
E Coli ◽  
Allium Chinense

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

scholarly journals Antimicrobial Activity ofCroton macrostachyusStem Bark Extracts against Several Human Pathogenic Bacteria

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Jackie K. Obey ◽  
Atte von Wright ◽  
Jimmy Orjala ◽  
Jussi Kauhanen ◽  
Carina Tikkanen-Kaukanen
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Scientific Evidence ◽  
Enterobacter Aerogenes ◽  
Stem Bark ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Traditional Use

In Kenya, leaves and roots fromCroton macrostachyusare used as a traditional medicine for infectious diseases such as typhoid and measles, but reports on possible antimicrobial activity of stem bark do not exist. In this study, the antibacterial and antifungal effects of methanol, ethyl acetate and butanol extracts, and purified lupeol ofC. macrostachyusstem bark were determined against important human gram-negative pathogensEscherichia coli,Salmonella typhi,Klebsiella pneumoniae, andEnterobacter aerogenes, gram-positiveListeria monocytogenes,and a fungusCandida albicans. The most promising broad scale antimicrobial activity against all the studied pathogens was shown by the ethyl acetate extract. The ethyl acetate extract induced the zone of inhibition between10.1±0.6 mm and16.0±1.2 mm againstS. typhi,E. coli,K. pneumoniae,E. aerogenes,andL. monocytogeneswith weaker antimicrobial activity againstC. albicans(zone of inhibition:5.6±1.0 mm). The antibiotic controls (amoxicillin, ciprofloxacin, ampicillin, benzylpenicillin, clotrimazole, and cefotaxime) showed antimicrobial activity with zones of inhibition within13.4±0.7–22.1±0.9 mm. The ethyl acetate extract had MIC in the range of 125–250 mg/mL against all the studied bacteria and againstC. albicansMIC was 500 mg/mL. The present results give scientific evidence and support the traditional use ofC. macrostachyusstem bark as a source for antimicrobials. We show thatC. macrostachyusstem bark lupeol is a promising antimicrobial agent against several important human pathogens.

scholarly journals Antimicrobial activity and phytochemical screening of Adansonia digitata stem bark extract on some clinical isolates

2020 ◽  
Vol 22 (2) ◽  
pp. 035-044
Author(s):  
A.E. Ajiboye ◽  
S.O. Sadiq ◽  
M.R. Adedayo
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Clinical Isolates ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Adansonia Digitata ◽  
Standard Methods ◽  
E Coli

Adansonia digitata is a massive and deciduous tree with a height of about 24 m and of significant economic importance. The antimicrobial and  phytochemical screening of the aqueous and ethyl-acetate extract of stem bark of the plant were determined on some clinical isolates. The stem bark of the plant was collected and washed o properly before drying at 28 C. The pulverised stem bark was extracted with water and ethyl-acetate and screened for phytochemicals (qualitative and quantitative) using standard methods. The clinical isolates used were identified as Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli and Candida albicans. The antimicrobial activities of the crude extracts were carried out using the agar well diffusion methods. The minimum inhibitory concentration (MIC), minimum bactericidal and fungicidal concentrations were carried out using standard methods. The aqueous extract exhibited a higher zone of inhibition against S. aureus (14.00 ± 0.57 mm) at a concentration of 200 mg/ml while a zone of inhibition of 11.66±0.33 mm was observed for E. coli using ethylacetate extract. Candida albicans had a zone of inhibition of 11.66±0.88 mm and 11.00±0.57 mm using aqueous and ethyl-acetate extracts respectively at 200 mg/ml. The MIC was 200 mg/ml for the crude extracts against the clinical isolates. The qualitative ethyl-acetate phytochemical screening revealed the presence of phenol, flavonoids, tannins, alkaloids, saponin and terpenoids. Phenol had the highest concentration of 2.02±0.25 mg/ml while terpenoids had a value of 1.38±0.02 mg/ml. Aqueous and ethyl-acetate extract of A. digitata possess significant antimicrobial activity against E. coli, S. aureus and C. albicans. However, K. pneumoniae showed resistance to ethyl acetate extract. Key words: Adansonia digitata, Antimicrobial activity, Clinical isolates, Phytochemical  screening

scholarly journals Antimicrobial and in vitro cytotoxic studies of Acampe praemorsa and Aeridis odarata of Orchidaceae

2018 ◽  
Vol 7 (2) ◽  
pp. 2088
Author(s):  
Jhansi Katta ◽  
Khasim S.M.
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Eastern Ghats ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

This paper aims at studying antimicrobial efficacy and in vitro cytoxic activity of epiphytic orchids namely Acampe praemorsa, Aeridis odorata distributed in Eastern Ghats of Visakhapatnam district. Plants were collected, identified following literature, shade dried and Methanol, ethyl acetate extracts were prepared for the systematic investigation of antimicrobial activity of plant extracts. Antibacterial activity against three gram positive bacteria Bacillus megaterium, Lactobacillus acidophillus and Enterococcus faecalis, three gram negative bacteria Proteus vulgaris, Klebsiella pneumoniae and Escherichia coli was done using Agar well diffusion method, antifungal activity was carried against Candida albicans, Aspergillus flavus by Cpzapak dox agar media, MIC by broth dilution method, zones of inhibition were recorded. Ethyl acetate extracts showed maximum antimicrobial activity against all bacteria and fungi, Aeridis odarata ethyl acetate extract showed highest zone of inhibition 17mm against Lactobacillus acidophilus in bacteria, Ethyl acetate extract of Acampe praemorsa showed highest zone of inhibition 17mm against Candida albicans in fungi, the leaf extracts were tested for its inhibitory effect on HeLa and MCF-7 cell lines were evaluated by the MTT assay and methanolic extract of Aeridis odarata has significant cytotoxicity effect on MCF-7 cell line in concentration range between 5 to 100µg/ml, with IC 50 (µg/ml) value is 26.2. These plants have good antimicrobial activity, further investigation on the phytochemistry of bioactive compounds of these plants would result in discovery of new drugs and further pharmacological investigation of anti-cancer activity of Aeridis odarata should be done.

scholarly journals A preliminary study on the antimicrobial activities and gas chromatography-mass spectrometry (GC-MS) analysis of the ethyl acetate extract of Dennettia tripetala G. Baker seeds

Bio-Research ◽  
2020 ◽  
Vol 18 (1) ◽  
pp. 1094-1102
Author(s):  
UF Babaiwa ◽  
SO Eraga ◽  
EO Ojugo ◽  
O Erharuyi ◽  
JO Akerele
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Fatty Acid Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Traditional Use ◽  
Dennettia Tripetala

The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.  

scholarly journals <i>In-vitro</i> antibacterial sensitivity of <i>Usnea barbata</i> lichen extracted with methanol and ethyl-acetate against selected <i>Staphylococcus</i> species from milk of cows with mastitis

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Emrobowansan Monday Idamokoro ◽  
Patrick Julius Masika ◽  
Voster Muchenje ◽  
Daniel Falta ◽  
Ezekiel Green
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Bovine Milk ◽  
Raw Milk ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Ethyl Acetate Extracts

Abstract. This study aimed at evaluating the antimicrobial potential of Usnea barbata lichen as a medicinal plant against selected Staphylococcus species isolated from raw milk of cows. In-vitro screening of methanol and ethyl-acetate extracts from Usnea barbata lichen were evaluated to determine their antimicrobial activity against thirteen different Staphylococcus species. The selected organisms were isolated from raw bovine milk and identified using several biochemical tests and confirmed with API staph kit. The antimicrobial activity of the extracts were evaluated using both the agar well diffusion method (at 5 mg/ml, 10 mg/ml and 20 mg/ml) and the broth micro-dilution technique to determine the mean zone of inhibition and the minimum inhibitory concentration (MIC), respectively. Both the methanol and ethyl-acetate extracts showed variable antimicrobial activity against the Staphylococcus species with mean zones of inhibition ranging from 0-34 mm in diameter at 5 mg/ml, 10 mg/ml and 20 mg/ml, respectively. Susceptibility by the Staphylococcus species tested in the methanol and the ethyl-acetate extract was 92.31 % and 53.85 %, respectively. The MIC result for the methanol extract ranged from 0.04 to 10 mg/ml, while that of the ethyl-acetate extract ranged from 0.16 to 5 mg/ml. Results from this study revealed the in vitro microbial activity of Usnea barbata extracts which indicate its potential as a medicinal plant.

scholarly journals Triterpenoids from the Stem Bark of Crataeva nurvala

1970 ◽  
Vol 7 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Md Enamul Haque ◽  
Md Nahidul Islam ◽  
Dipankar Das Gupta ◽  
Mahbub Hossain ◽  
Hossain Uddin Shekhar ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Key Words ◽  
Silica Gel ◽  
1H Nmr ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
First Report ◽  
Spectroscopic Analyses ◽  
Phytochemical Investigation

Two triterpenoids, phragmalin triacetate (1) and lupeol (2) were isolated from an ethyl acetate extract of the stem bark of Crataeva nurvala (Capparidaceae) by repeated chromatography over silica gel. The structures of these compounds were determined by spectroscopic analyses (UV, IR, 1H NMR, 13C NMR and EIMS). This is the first report of the systematic phytochemical investigation and the presence of these compounds 1 and 2 from this plant. Key words: Crataeva, Capparidaceae, Phragmalin triacetate and Lupeol.  DOI = 10.3329/dujps.v7i1.1221 Dhaka Univ. J. Pharm. Sci. 7(1): 71-74, 2008 (June)

scholarly journals Studies on comparative antimicrobial activities of Aerva lanata and Momordica charantia leaf extracts

2020 ◽  
pp. 561-564
Author(s):  
Nagula Shilpika ◽  
Byagari Teena Keerthana ◽  
Banka Srinivas ◽  
Gampa Tulja Rani ◽  
Ganga Maithili
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antimicrobial Activities ◽  
Momordica Charantia ◽  
Leaf Extracts ◽  
Plate Method ◽  
Methanolic Extracts ◽  
Aerva Lanata

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

scholarly journals Antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus

2016 ◽  
Vol 1 (2) ◽  
pp. 29 ◽  
Author(s):  
M. Pathmavathi ◽  
P. Thamizhiniyan
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Fungal Pathogens ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Phyllanthus Amarus ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Aspergillus Fumigates

Hexane, chloroform, ethyl acetate and methanol extracts of leaves of Plectranthus ambionicus and  Phyllanthus amarus were screened for their antimicrobial activity against human pathogenic bacterial and fungal strains. Antimicrobial activity was carried out by disc diffusion method, determination of minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC) and minimum fungicidal concentrations (MFC) against four strains of Gram positive bacteria, three strains of Gram negative bacteria and three species of fungi. The antimicrobial activity of various extracts of Plectranthus ambionicus and Phyllanthus amarus showed varied levels of antimicrobial activity against the studied bacterial and fungal pathogens. The mean zone of inhibition produced by all the tested extracts ranged from 7.0 ± 0.50 mm to 29.5 ± 0.50 mm. The MIC, MBC and MFC values were between 15.62 and 1000 μg/mL. The ethyl acetate extract of Plectranthus ambionicus showed good antimicrobial activity with the highest mean zone of inhibition (29.5±0.5 mm), lowest MIC (15.62 μg/mL) and MBC (31.25 μg/mL) values followed by ethyl acetate extract of Phyllanthus amarus(23.6±0.50 mm; MIC=62.5; MBC=125 μg/mL) against Aspergillus fumigates.

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