Identification of Locally Isolated Cellulolytic Stenotrophomonas maltophilia from Rice Straw and Optimization of its Cellulase Activity

2020 ◽  
Vol 37 (1) ◽  
pp. 15-22
Author(s):  
Faozia Faleha Sadida ◽  
Fatima Binte Kamal ◽  
Mohammed Abul Manchur
Keyword(s):  
Nitrogen Source ◽  
Rice Straw ◽  
Stenotrophomonas Maltophilia ◽  
Cellulase Activity ◽  
Cellulolytic Bacterium ◽  
Carbon And Nitrogen ◽  
Cmcase Activity ◽  
Sds Page ◽  
Extracellular Protein Production ◽  
Rdna Analysis

A highly cellulolytic bacterium was locally isolated from rice straw and identified as Stenotrophomonas maltophilia. Identification of the isolate based on the morphological, cultural and biochemical characteristics was confirmed with 16S rDNA analysis. The bacterium showed the highest level of reducing sugar and extracellular protein production when incubated for 3 days (348.75 μg/ml and 288.5 μg/ml respectively) at 40°C temperature (463.0 μg/ ml and 333.0 μg/ml respectively) and pH 6.5 (360.0 μg/ml and 349.0 μg/ml respectively) in Winstead’s broth having 1.5% CMC and 0.2% Yeast Extract as carbon and nitrogen source respectively. Crude cellulase enzymes produced by the bacterium showed the highest CMCase activity rather than FPase, Avicelase and ²-Glucosidase activities. Cellulase activity of the crude enzyme was also determined using the same parameters. The crude cellulase enzyme showed the highest CMCase activity when incubated for 60 minutes (232.5 U/ml), at pH 6.5 (105.0 U/ml), 35°C temperature (69.75 U/ml) using CMC and Peptone as carbon and nitrogen source respectively. Crude cellulase showed the highest activity in presence of mercury and SDS as metal and detergent respectively. Substrate specificity and SDS-PAGE analysis reveals that the cellulase may be an endo-1,4-glucanase. Bangladesh J Microbiol, Volume 37 Number 1 June 2020, pp 15-22

Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw

2021 ◽  
Vol 50 (2) ◽  
pp. 395-404
Author(s):  
Faozia Faleha Sadida ◽  
Ma Manchur
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Rice Straw ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Tetraacetic Acid ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Ph

A highly cellulolytic actinomycete SR1 was locally isolated from rice straw and provisionally identified as Thermomonospora viridis. Optimum pH, temperature, carbon and nitrogen sources for its cellulase production were 6.5, 35°C, Carboxymethyl cellulase (CMC) and yeast extract, respectively whereas those of cellulase activity were 7.5, 40°C, CMC and peptone respectively. The effects of various metal ions and different reductant and inhibitors on its cellulase activity were investigated. Univalent Ag+ was found to decrease the enzyme activity whereas increased by bivalent Mg2+. Ethylene diamine tetraacetic acid (EDTA) caused remarkable decrease of cellulase activity but β-Mercaptoethanol stimulated its cellulase activity. Bangladesh J. Bot. 50(2): 395-404, 2021 (June)

scholarly journals Carboxymethyl cellulase production from a Paenibacillus sp.

2016 ◽  
Vol 70 (3) ◽  
pp. 329-338 ◽  
Author(s):  
Katarina Mihajlovski ◽  
Sladjana Davidovic ◽  
Milica Carevic ◽  
Neda Radovanovic ◽  
Slavica Siler-Marinkovic ◽  
...  
Keyword(s):  
Culture Supernatant ◽  
Soluble Sugars ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Carboxymethyl Cellulase ◽  
Carbon And Nitrogen ◽  
Cmcase Activity ◽  
Working Volume ◽  
Paenibacillus Sp ◽  
Initial Ph

Cellulases are industrially important enzymes with a potential to convert cellulose into fermentable sugars. Novel bacterial isolate Paenibacillus sp. CKS1 was tested for cellulase activity and the optimal conditions for carboxymethyl cellulase (CMCase) production were determined. Maximum CMCase activity was obtained in the third passage of the bacterial culture after 3 days of incubation at 30?C. Cellobiose and yeast extract was the optimal source of carbon and nitrogen for induction of CMCase activity. In addition, with initial pH 7 of the medium and 40 ml of working volume in 500 ml culture flasks with shaking at 150 rpm, the maximum CMCase activity in a crude culture supernatant reached value of 0.532?0.006 U/ml. For crude CMCase, optimal temperature was 50?C and optimal pH 4.8, respectively. HPLC analysis confirmed the bacterium is capable to hydrolise CMC to glucose and other soluble sugars.

Access to 2-Arylquinazolines via Catabolism/Reconstruction of Amino Acids with Insertion of Dimethyl Sulfoxide

2021 ◽  
Author(s):  
Jin-Tian Ma ◽  
Li-Sheng Wang ◽  
Zhi Chai ◽  
Xin-Feng Chen ◽  
Bo-Cheng Tang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dimethyl Sulfoxide ◽  
Nitrogen Source ◽  
Carbon And Nitrogen ◽  
First Time

Quinazoline skeletons are synthesized by amino acids catabolism/reconstruction combined with dimethyl sulfoxide insertion/cyclization for the first time. The amino acid acts as a carbon and nitrogen source through HI-mediated catabolism...

Facile preparation of C, N co-modified Nb2O5 nanoneedles with enhanced visible light photocatalytic activity

2016 ◽  
Vol 45 (41) ◽  
pp. 16519-16525 ◽  
Author(s):  
Jiao Xue ◽  
Runwei Wang ◽  
Zongtao Zhang ◽  
Shilun Qiu
Keyword(s):  
Photocatalytic Activity ◽  
Visible Light ◽  
Nitrogen Source ◽  
Hydrothermal Method ◽  
Niobium Pentoxide ◽  
Facile Hydrothermal Method ◽  
Carbon And Nitrogen ◽  
Niobium Chloride ◽  
Facile Preparation ◽  
Visible Light Photocatalytic

C, N co-modified niobium pentoxide (Nb2O5) nanoneedles have been successfully synthesized via a facile hydrothermal method with Niobium Chloride (NbCl5) as a precursor and triethylamine as both the carbon and nitrogen source.

scholarly journals Production of Feruloyl Esterase from Aspergillus niger by Solid-State Fermentation on Different Carbon Sources

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Shiyi Ou ◽  
Jing Zhang ◽  
Yong Wang ◽  
Ning Zhang
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Wheat Bran ◽  
Cell Walls ◽  
Carbon Sources ◽  
Feruloyl Esterase ◽  
Plant Cell Walls ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Maize Bran

A mixture of wheat bran with maize bran as a carbon source and addition of (NH4)SO4 as nitrogen source was found to significantly increase production of feruloyl esterase (FAE) enzyme compared with wheat bran as a sole carbon and nitrogen source. The optimal conditions in conical flasks were carbon source (30 g) to water 1 : 1, maize bran to wheat bran 1 : 2, (NH4)SO4 1.2 g and MgSO4 70 mg. Under these conditions, FAE activity was 7.68 mU/g. The FAE activity on the mixed carbon sources showed, high activity against the plant cell walls contained in the cultures.

Straw management, crop rotation and nitrogen source effect on carbon and nitrogen dynamics: A laboratory study

2009 ◽  
Vol 325 (1-2) ◽  
pp. 243-253 ◽  
Author(s):  
A. Montoya-González ◽  
O. E. González-Navarro ◽  
B. Govaerts ◽  
K. D. Sayre ◽  
I. Estrada ◽  
...  
Keyword(s):  
Nitrogen Source ◽  
Crop Rotation ◽  
Laboratory Study ◽  
Nitrogen Dynamics ◽  
Straw Management ◽  
Source Effect ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Dynamics

scholarly journals A Mannose Family Phosphotransferase System Permease and Associated Enzymes Are Required for Utilization of Fructoselysine and Glucoselysine in Salmonella enterica Serovar Typhimurium

2015 ◽  
Vol 197 (17) ◽  
pp. 2831-2839 ◽  
Author(s):  
Katherine A. Miller ◽  
Robert S. Phillips ◽  
Paul B. Kilgore ◽  
Grady L. Smith ◽  
Timothy R. Hoover
Keyword(s):  
Nitrogen Source ◽  
Nitrogen Sources ◽  
Maillard Reaction Products ◽  
Reaction Products ◽  
Phosphotransferase System ◽  
Carbon And Nitrogen Sources ◽  
Content Type ◽  
Carbon And Nitrogen ◽  
Serovar Typhimurium ◽  
Food Borne Illness

ABSTRACTSalmonella entericserovar Typhimurium, a major cause of food-borne illness, is capable of using a variety of carbon and nitrogen sources. Fructoselysine and glucoselysine are Maillard reaction products formed by the reaction of glucose or fructose, respectively, with the ε-amine group of lysine. We report here thatS. Typhimurium utilizes fructoselysine and glucoselysine as carbon and nitrogen sources via a mannose family phosphotransferase (PTS) encoded bygfrABCD(glucoselysine/fructoselysine PTS components EIIA, EIIB, EIIC, and EIID; locus numbers STM14_5449 to STM14_5454 inS. Typhimurium 14028s). Genes coding for two predicted deglycases within thegfroperon,gfrEandgfrF, were required for growth with glucoselysine and fructoselysine, respectively. GfrF demonstrated fructoselysine-6-phosphate deglycase activity in a coupled enzyme assay. The biochemical and genetic analyses were consistent with a pathway in which fructoselysine and glucoselysine are phosphorylated at the C-6 position of the sugar by the GfrABCD PTS as they are transported across the membrane. The resulting fructoselysine-6-phosphate and glucoselysine-6-phosphate subsequently are cleaved by GfrF and GfrE to form lysine and glucose-6-phosphate or fructose-6-phosphate. Interestingly, althoughS. Typhimurium can use lysine derived from fructoselysine or glucoselysine as a sole nitrogen source, it cannot use exogenous lysine as a nitrogen source to support growth. Expression ofgfrABCDEFwas dependent on the alternative sigma factor RpoN (σ54) and an RpoN-dependent LevR-like activator, which we designated GfrR.IMPORTANCESalmonellaphysiology has been studied intensively, but there is much we do not know regarding the repertoire of nutrients these bacteria are able to use for growth. This study shows that a previously uncharacterized PTS and associated enzymes function together to transport and catabolize fructoselysine and glucoselysine. Knowledge of the range of nutrients thatSalmonellautilizes is important, as it could lead to the development of new strategies for reducing the load ofSalmonellain food animals, thereby mitigating its entry into the human food supply.

scholarly journals Screening for the Production and Characterization of L-asparaginase from Endophytic Fungi

2021 ◽  
Vol 8 (5) ◽  
pp. 168-173
Author(s):  
Harjeet Singh ◽  
Shweta Sao
Keyword(s):  
Lymphoblastic Leukemia ◽  
Fungal Endophytes ◽  
Erwinia Carotovora ◽  
Maximum Activity ◽  
Phenol Red ◽  
Human Beings ◽  
Ph Indicator ◽  
Carbon And Nitrogen ◽  
Sds Page ◽  
Secondary Neoplasm

L-asparaginase (EC 3.5.11. L-asparagine amidohydrolase) is first enzyme, studied very intensively in human beings with regard to its anti-tumor potential against tumor of lymphoid precursor, acute lymphoblastic leukemia (ALL). The current drugs are suffering from many side effects like immune suppression, infertility, secondary neoplasm. The immunogenic complications associated with its present microbial sources Escherichia coli; Erwinia carotovora limits its medicinal frontier. So there exists a need of switching to novel natural sources to serve as non-immunogenic and better production sources of L-asparaginase. In the present study, four cultures of fungal endophytes viz. TSF-1, TSF-2, TSF-3 and TSF-4 selected on the basis of primary and secondary screening was carried on with L-asparagine as a sole carbon and nitrogen source and phenol red as pH indicator. The maximum protein content was observed to be present in TSF-2 i.e. 2.727 mg /mL and possessed maximum activity of 6.054 Units/ml. Sample was separated by SDS-PAGE, stained by silver staining, showed a single band with molecular weight of approximately ~45kDa.

scholarly journals Production and characterization of rhamnolipids from Pseudomonas aeruginosa san ai

2012 ◽  
Vol 77 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Milena Rikalovic ◽  
Gordana Gojgic-Cvijovic ◽  
Miroslav Vrvic ◽  
Ivanka Karadzic
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Response Surface Methodology ◽  
Nitrogen Source ◽  
Testing Effect ◽  
Carbon And Nitrogen ◽  
Rhamnolipid Production ◽  
Proteose Peptone ◽  
Isolated Compound ◽  
Rhamnolipid Biosurfactant

Production and characterization of rhamnolipid biosurfactant obtained by strain Pseudomonas aeruginosa san ai was investigated. With regard to carbon and nitrogen source several media were tested to enhance production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sun flower oil as a source of carbon and mineral ammonium chloride and peptone as a nitrogen source greatly improved rhamnolipid production, from 0.15 on basic PPAS (C/N ratio 4.0), to 3 g L-1, on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing effect of three factors: temperature, concentration of carbon and nitrogen source (w/w), in optimized PPAS medium on rhamnolipid production. Isolated rhamnolipids were characterized by IR and ESI-MS. IR spectra confirmed that isolated compound corresponds to rhamnolipid structure, whereas MS indicated that isolated preparation is a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic- and dirhamno- di-lipidic congeners.

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