scholarly journals Phytochemical investigation GC-MS analysis and in vitro antimicrobial activity of Coleus forskohlii

2015 ◽  
Vol 10 (4) ◽  
pp. 924 ◽  
Author(s):  
Krishnamoorthy Rajkumar ◽  
Ramaswamy Malathi
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Coleus Forskohlii ◽  
Ms Analysis ◽  
Phytochemical Investigation

<p class="Abstract">The aim of this study was to investigate the phytochemical constituents, gas chromatography-mass spectrometry (GC-MS) analysis and antimicrobial activity of <em>Coleus forskohlii</em>. The different solvents such as ethanol, chloroform, acetone and aqueous extracts were identified pharmacologically as important bioactive compounds and their antimicrobial properties were studied. In the phytochemical investigation almost all the ethanol extract of leaf, stem and root having secondary metabolites like alkaloids, flavonoids, tannins, saponins, terpenoids, and steroids. The active constituents of the ethanol extract of <em>C. forskohlii</em> root was studied by GC-MS analysis. According to the antimicrobial results ethanol extract of <em>C. froshkolii</em> root showed highest antibacterial activity compared with stem and leaf. The highest antimicrobial activity was observed against <em>Klebsiella pneumonia</em> (19 mm) and <em>Candida albicans </em>(16 mm) in ethanol extract of root. Among the above extracts of leaf, stem and root, ethanol extract of root having antimicrobial activities due to the presence of phytoconstituents.</p><p class="FigureLegend"> </p>

Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 52-58
Author(s):  
Elizabeth Winful ◽  
Olanikpekun Idowu ◽  
Opeoluwa O. Fasanya ◽  
Nkechi E. Egbe
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Garcinia Kola ◽  
Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

2017 ◽  
Vol 10 (11) ◽  
pp. 109
Author(s):  
Krishnavignesh L Krishnavignesh ◽  
Mahalakshmipriya A ◽  
Ramesh M
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

  Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

scholarly journals Green propolis as an adjuvant against nontuberculous mycobacteria

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Suzane Olachea Allend ◽  
Lisiane Volcão ◽  
Carolina da Silva Canielles ◽  
Israel Barbosa ◽  
Dara Biatobock ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Nontuberculous Mycobacteria ◽  
Ethanol Extract ◽  
Antimycobacterial Activity ◽  
Antimicrobial Activities ◽  
Resistance Mechanisms ◽  
The Other ◽  
New Drugs ◽  
Mycobacterium Chelonae

Abstract Natural products have been touted as important tools because of their vast potential for the development of compounds with antimicrobial activity and the possible inhibitory activity and/or adjuvant resistance mechanisms. Propolis has been empirically used for many years for the treatment of diseases, mainly due to its antioxidant, anti inflammatory and antimicrobial activities. This study aimed to evaluate the in vitro antimycobacterial activity of the ethanol extract of propolis alone and in combination with rifampicin (RIF), amikacin (AMI) and ciprofloxacin (CIP). The ethanol extract of propolis showed antibacterial activity against Mycobacterium chelonae and M. kansasii and was capable of increasing AMI, RIF and CIP activity in combination. On the other hand, compared to M. absecessus, M. fortuitum and M. avium, the extract was not active at 200 µg/mL and did not show pronounced adjuvant capacity when evaluated in association with the drugs. Based on these results, it can be concluded that the ethanol extract of propolis could be an alternative in the development of new drugs and can be used complementary with the current mycobacteriosis treatment.

scholarly journals In vitro antimicrobial screening of Lycoperdon lividium and determination of the ethanol extract composition by gas chromatography/mass spectrometry

2016 ◽  
Vol 11 (2) ◽  
pp. 389 ◽  
Author(s):  
Kerem Canli ◽  
Ergin Murat Altuner ◽  
Ilgaz Akata ◽  
Yavuz Turkmen ◽  
Ugur Uzek
Keyword(s):  
Antimicrobial Activity ◽  
Video Clip ◽  
Ethanol Extract ◽  
Disk Diffusion ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Disk Diffusion Test ◽  
Wide Range

<p class="Abstract">The aim of this study was to investigate the antimicrobial activity of <em>Lycoperdon lividium</em> against 17 bacterial and 1 fungal strains and analyse the composition of ethanol extracts by GC/MS. The in vitro antimicrobial activity of <em>L. lividium</em> extracts having 15 mg/mL concentration was assessed against a wide range of strains by disk diffusion method. The ethanol extract of <em>L. lividium</em> had antimicrobial activity against several microorganism tested, but it was active especially against <em>S. carnosus</em>. The results obtained herein indicate that <em>L. lividium</em> contains several active metabolites.</p><p class="Abstract">Video clip</p><p><a href="https://youtube.com/v/ymWOQBeNN84">Disk diffusion test</a>: 7 min 11 sec</p>

scholarly journals In vitro antimicrobial activity of douro wines against clinical Helicobacter Pylori Strains

Nova ◽  
2021 ◽  
Vol 19 (37) ◽  
pp. 121-134
Author(s):  
Lidia Po Catalao Dionisio ◽  
Alejandro Manuel Labella ◽  
María Palma ◽  
Juan José Borrego
Keyword(s):  
Antimicrobial Activity ◽  
Helicobacter Pylori ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Horse Blood ◽  
H Pylori

Aim. In vitro antimicrobial activities of seven wines (5 reds and 2 whites) from the Douro region (Iberian Peninsule) against eleven clinical strains of Helicobacter pylori were evaluated. Methods. The disk diffusion method, using Columbia Agar supplemented with horse blood (CAB), were used to determine the antimicrobial properties of some wine components against H. pylori strains. Potential interactions of antioxidants contained in the wines and two antimicrobials (amoxicillin and metronidazole) were studied by the disk diffusion method. Results. All the tested strains showed growth in CAB supplemented with 9% of the tested wines but none of them grew in media supplemented with 45% and 67.5% of wine. Similarly, all the tested strains grew in media with the concentration of proanthocyanidins present in the different types of the studied wines. The Minimal Inhibitory Concentration (MIC) values of the wine antioxidant components tested (benzoic acid, catechin, quercetin, and resveratrol) indicate that resveratrol was the most powerful inhibitory substance against H. pylori. An effect of potentiation between amoxicillin and metronidazole and the antioxidants tested was also established. The interaction of amoxicillin and resveratrol or metronidazole and catechin increased the antimicrobial activity against H. pylori. Conclusions. The results obtained suggested a potential role of resveratrol as a chemopreventive agent for H. pylori infection.

In vitro antimicrobial activity of the leaf extract of Harungana madagascariensis Lam. ex Poir. (Hypericaceae) against strains causing otitis externa in dogs and cats

2007 ◽  
Vol 55 (1) ◽  
pp. 97-105 ◽  
Author(s):  
B. Moulari ◽  
Y. Pellequer ◽  
J. Chaumont ◽  
Y. Guillaume ◽  
J. Millet
Keyword(s):  
Otitis Externa ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Malassezia Pachydermatis ◽  
Staphylococcus Intermedius ◽  
Phytochemical Investigation

Otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are Malassezia pachydermatis, Staphylococcus intermedius and Pseudomonas species. These organisms often develop resistance to classical antimicrobial agents. Therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of Harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of this extract and to determine its bioactive chemical class using dilution techniques, the bioautography method and the standard phytochemical method described by Harborne (1973). Phytochemical analysis revealed the presence of saponins, tannins, flavonoids, alkaloids and anthracenic derivatives. The bioassay showed that the antimicrobial properties may be attributed to astilbin, a flavanone derivative identified on the basis of its spectroscopic data. The results suggest that the extract could be used in an antimicrobial preparation effective against the whole range of organisms incriminated in otitis externa in dogs and cats, with a minimal inhibitory concentration (MIC) of 250 μg/ml.

Composition and Antimicrobial Properties of Sardinian Juniperus Essential Oils against Foodborne Pathogens and Spoilage Microorganisms

2003 ◽  
Vol 66 (7) ◽  
pp. 1288-1291 ◽  
Author(s):  
SOFIA COSENTINO ◽  
ANDREA BARRA ◽  
BARBARA PISANO ◽  
MADDALENA CABIZZA ◽  
FILIPPO MARIA PIRISI ◽  
...  
Keyword(s):  
Essential Oils ◽  
Foodborne Pathogens ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Aflatoxin Contamination ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Microdilution Method ◽  
Ms Analysis ◽  
Broth Microdilution Method

In this work, the chemical compositions and antimicrobial properties of Juniperus essential oils and of their main components were determined. Five berry essential oils obtained from different species of Juniperus growing wild in Sardinia were analyzed. The components of the essential oils were identified by gas chromatography–mass spectrometry (GC-MS) analysis. The antimicrobial activities of the oils and their components against food spoilage and pathogenic microorganisms were determined by a broth microdilution method. The GC-MS analysis showed a certain variability in the concentrations of the main constituents of the oils. α-Pinene was largely predominant in the oils of the species J. phoenicea subsp. turbinata and J. oxycedrus. α-Pinene and myrcene constituted the bulk (67.56%) of the essential oil of J. communis. Significant quantitative differences were observed for myrcene, δ-3-carene, and d-germacrene. The results of the antimicrobial assay show that the oils of J. communis and J. oxycedrus failed to inhibit any of the microorganisms at the highest concentrations tested (MLC ≥ 900 μg/ml), while the oils extracted from J. turbinata specimens were active against fungi, particularly against a strain of Aspergillus flavus (an aflatoxin B1 producer). Of the single compounds tested, δ-3-carene was found to possess the broadest spectrum of activity and appeared to contribute significantly to the antifungal activity observed for J. turbinata oils. This activity may be helpful in the prevention of aflatoxin contamination for many foods.

scholarly journals GC-MS PROFILE OF IN VIVO AND IN VITRO SHOOTS OF CLEOME GYNANDRA L.

2017 ◽  
Vol 9 (10) ◽  
pp. 21
Author(s):  
Karthikeyan A. V. P. ◽  
Sudan I.
Keyword(s):  
Biological Activities ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Medium ◽  
Shoot Induction ◽  
In Vitro Plants ◽  
Ms Analysis ◽  
Cleome Gynandra

Objective: Investigation of the bioactive compounds from the ethanol shoot extracts of in vivo and in vitro plants of Cleome gynandra (C. gynandra) through GC-MS analysis. Methods: The nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl-aminopurine (BAP), kinetin-6-furfurylaminopurine (Kin) and indole 3 acetic acids (IAA) for shoot induction. In the present study, the phytochemical constituents were analyzed from the ethanol extract of in vivo and in vitro plants of C. gynandra using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The mass spectrum of the ethanol extract was compared with the available library sources.Results: In the present study, different concentrations of cytokinins and in the combination of IAA are used to develop regenerated shoots. The maximum number of shoots was obtained 9.2±0.41 with the length of 6.6 cm and highest frequency of (100%) shoot induction was observed on MS medium containing 10 μM BAP with 4 μM IAA. The GC-MS analysis revealed that the shoots of in vivo and in vitro plants contained 21phytochemicals, of these 3 components were similar in both in vivo and in vitro plants, 2 phytochemical's are repeated with different RT, 7 components are having biological activity and in the remaining 9 components, biological activities are not reported.Conclusion: The present study, the in vitro regeneration, combinations of hormones (10 μM BAP plus 4 μM IAA) tested showed the best result than individual and also revealed that the synthesis of more number of phytochemicals present in the ethanolic extracts of in vitro plants than the in vivo plants of C. gynandra.

Dicentra scandens (D.Don) Walp. - A Potential Source of Antimicrobial Agent

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Koirala Pramila ◽  
Singh Bimala
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Ms Analysis

Dicentra scandens (D.Don) Walp. locally called as ‘Jogi Lahara’ belongs to the Family Fumariaceae and is used in traditional medicine in Sikkim, a North Eastern state of India. The present study investigated the antimicrobial properties of aqueous and methanol extracts of Dicentra scandens (D.Don) Walp. against some test Gram negative and Gram positive bacteria. Using agar well diffusion method, aqueous and methanol extracts of roots of Dicentra scandens were tested against Escherichia coli (MTCC 1089), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 1034), Proteus vulgaris (MTCC 742), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio cholerae O139(MTCC 3906), Bacillus cereus (MTCC 6840) and Staphylococcus aureus (MTCC 7443). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also evaluated. Promising antimicrobial activity was exhibited by methanol extract of Dicentra scandens. The methanol extract was further characterized by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The GC-MS analysis revealed 29 compounds and the major compounds detected were Protopine (53.78%) and Corydine (18.20%). Thus, the alkaloids are predominant phytoconstituents of the extract and could be attributed to its antimicrobial activity. The results of the present study indicate that, Dicentra scandens can be a source of potential antimicrobial agent and can be explored further for its therapeutic use.

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