Dicentra scandens (D.Don) Walp. - A Potential Source of Antimicrobial Agent

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Koirala Pramila ◽  
Singh Bimala
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Ms Analysis

Dicentra scandens (D.Don) Walp. locally called as ‘Jogi Lahara’ belongs to the Family Fumariaceae and is used in traditional medicine in Sikkim, a North Eastern state of India. The present study investigated the antimicrobial properties of aqueous and methanol extracts of Dicentra scandens (D.Don) Walp. against some test Gram negative and Gram positive bacteria. Using agar well diffusion method, aqueous and methanol extracts of roots of Dicentra scandens were tested against Escherichia coli (MTCC 1089), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 1034), Proteus vulgaris (MTCC 742), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio cholerae O139(MTCC 3906), Bacillus cereus (MTCC 6840) and Staphylococcus aureus (MTCC 7443). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also evaluated. Promising antimicrobial activity was exhibited by methanol extract of Dicentra scandens. The methanol extract was further characterized by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The GC-MS analysis revealed 29 compounds and the major compounds detected were Protopine (53.78%) and Corydine (18.20%). Thus, the alkaloids are predominant phytoconstituents of the extract and could be attributed to its antimicrobial activity. The results of the present study indicate that, Dicentra scandens can be a source of potential antimicrobial agent and can be explored further for its therapeutic use.

scholarly journals Exploiting the Potential of Moringa oleifera Oil/Polyvinyl Chloride Polymeric Bionanocomposite Film Enriched with Silver Nanoparticles for Antimicrobial Activity

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Musarat Amina ◽  
Nawal M. Al Musayeib ◽  
Nawal A. Alarfaj ◽  
Maha F. El-Tohamy ◽  
Hisham E. Orabi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Polyvinyl Chloride ◽  
Moringa Oleifera ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
X Ray ◽  
Different Strains

The present study focused on the prospect of fabricating a polymeric naturally extracted Moringa oleifera oil bionanocomposite film enriched with silver nanoparticles for antimicrobial activity. In this study, a standard concentration of Moringa oleifera oil (5-10 wt%) was used to fabricate a polymeric bionanocomposite film using polyvinyl chloride (PVC) enriched with silver nanoparticles. The active constituents of the extracted Moringa oleifera oil were verified using gas chromatography-mass spectrometry. Spectroscopic and microscopic techniques, including scanning electron microscopy, X-ray diffraction, and energy-dispersive X-ray analysis, were employed to characterize and study the surface morphology of the fabricated bionanocomposite film. The antimicrobial activity of the fabricated bionanocomposite film was investigated using different strains of bacteria and fungus. The results revealed well-oriented and excellently dispersed silver nanoparticles in the PVC-Moringa oleifera oil matrix. The bionanocomposite was able to inhibit the growth of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa, Shigella flexneri, and Candida albicans. The combination of nanoparticles with polymers is opening new routes for engineering fixable composites, which showed antimicrobial properties.

scholarly journals Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Siti Samiyarsih ◽  
NUR FITRIANTO ◽  
ELLY PROKLAMASININGSIH ◽  
JUWARNO ◽  
JUNI SAFITRI MULJOWATI
Keyword(s):  
Antimicrobial Activity ◽  
Catharanthus Roseus ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
E Coli ◽  
Phytochemical Compounds ◽  
Dark Pink

Abstract. Samiyarsih S, Fitrianto N, Proklamasiningsih E, Juwarno, Muljowati JS. 2020. Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS. Biodiversitas 21: 1332-1344. Catharanthus roseus (L.) G is an important medicinal plant to evaluate the possibility of novel pharmaceuticals since most of the bacterial pathogens are developing resistance against antibiotics. This research aimed to determine the phytochemical diversity of methanol extract of eight cultivars of C. roseus and to evaluate for possible antimicrobial (antifungal and antibacterial) activities. It is the first research to compare phytochemicals and antimicrobial potential among C. roseus cultivars. The compound obtained was screened by Gas Chromatography-Mass Spectrometry (GC-MS) method. While agar-well disc diffusion method was employed to measure antimicrobial activity against Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Aspergillus niger. Based on GC-MS analysis, a total of 18 significantly different metabolite compounds. The abundances of phytochemical compounds (18 classes total) in each cultivar were Dark Pink (66%), Pink (50%), Purple Pink (44%), Pale Pink (27%), White (44%), Milky White (50%), Whitish pink (50%) and Pinkish Red (55%). The leaves extracts showed antimicrobial activity with inhibition zones ranging from 6.40-22.00 mm and 3.35-8.20 mm, respectively. The best antimicrobial activity against E. coli, S. aureus, A. flavus, and A. niger with the zone of inhibition 16.10±1.67 mm, 22.00±0.33 mm, 6.05±0.67 mm and 8.20±0.50 mm respectively by Dark Pink cultivar.

scholarly journals Synthesis, Characterization and Antimicrobial Activity of d8-10 Metal Complexes of some 2-Substituted-1H-Benzimidazoles

1999 ◽  
Vol 6 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Bahri Ülküseven ◽  
Aydin Tavman ◽  
Gülten Ötük
Keyword(s):  
Antimicrobial Activity ◽  
Metal Complexes ◽  
Staphylococcus Epidermidis ◽  
Shigella Flexneri ◽  
Analytical Data ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

The metal complexes of nine 2-substituted-1H-benzimidazoles (I-IX) with Ni(II), Pd(II), Cu(II), Ag(I), Zn(II) salts were synthesized. The compounds were characterized by melting point, analytical data, IR spectroscopy and magnetic susceptibility. The antimicrobial activity of the compounds was determined by the disk diffusion method in Mueller-Hinton Agar on Staphylococcus aureusATCC 6538, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 8739, Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 1539, Salmonella typhi, Shigella flexneri, Proteus mirabilis, Candida albicans ATCC 10231. Cu(II)and Ag(I)complexes of II, III and IV showed considerable activity against S. aureus, S. epidermidis, Ps. aeruginosa, S. typhi, Sh. flexneri and C. albicans microorganisms, the ligands themselves having no effect.

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS AND IN VITRO ANTIMICROBIAL SCREENING OF WEDELIA GLAUCA (ORTEGA) O. HOFFM. EX HICKEN

2017 ◽  
Vol 10 (11) ◽  
pp. 109
Author(s):  
Krishnavignesh L Krishnavignesh ◽  
Mahalakshmipriya A ◽  
Ramesh M
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

  Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens.Methods: The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant.Conclusion: W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

scholarly journals Phytochemical Screening and in vitro Antimicrobial Activity of Cyperus rotundus Leaf Extracts

2021 ◽  
Vol 4 (1) ◽  
pp. 43-52
Author(s):  
EO Agbo ◽  
JO Ogenyi ◽  
CA Adah ◽  
CT Agber
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethyl Acetate ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Cyperus Rotundus ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Study Results

Cyperus rotundus, a member of family Cyperaceae is a perennial, stoloniferous herb. The study investigated the secondary metabolites present in the leaf of Cyperus rotundus and antimicrobial activities of the hexane, ethyl acetate and methanol extracts. Phytochemical analysis revealed the presence of saponins, alkaloids, phenolic compounds, tannins, steroids, flavonoids, cardiac glycosides and terpenoids. The qualitative test of the extracts show the presence of steroid only in the hexane extract; flavonoid, cardiac glycoside in both the ethyl acetate and methanol extracts; saponin and tannin and phenolics in just the methanol extract. While alkaloid was present in all the extracts, terpenoid was absent in all. Antimicrobial activity of plant extracts were monitored using the agar disk-diffusion method against strains gram-positive bacterial: Staphylococcus aureus, Pseudomonas aeruginosa; and gram-negative bacteria: Escherichia coli, Salmonela typhi, and strains of fungi: Candida albicans and Aspergillus niger. The antimicrobial activity of the extracts was monitored at different concentrations of 50, 25, 12.5 and 6.25 mg/mL prepared from the stock concentration (100mg/mL) by serial dilution; with standard drugs: ciprofloxacin and fluconazole. The inhibition of the test organisms were concentration dependent. The methanol extract showed significantly higher Inhibition Zone (IZ) and Activity Index (AI) against the microbes at all concentrations compared to the ethyl acetate and hexane extracts due to the much phytochemicals (saponins, tanins, phenols, flavonoids, cardiac glycocides and alkaloids) found present in its extract. The overall study results signify the potential of Cyperus rotundus as a source of therapeutic agents. The methanol extract showed the best activity against both bacterial and fungal pathogens at the highest concentration of 100 mg/mL, especially the most susceptible bacteria: Staphylococcus aureus (IZ of 25.0±1.5; AI of 0.96) and Candida albicans (IZ of 25.5±0.5; AI of 0.91). At MIC, MBC and MFC of 6.25 mg/mL and 12.5 mg/mL, the plant extracts showed effective bactericidal and fungicidal effect against the test microbes.

scholarly journals Phytochemical investigation GC-MS analysis and in vitro antimicrobial activity of Coleus forskohlii

2015 ◽  
Vol 10 (4) ◽  
pp. 924 ◽  
Author(s):  
Krishnamoorthy Rajkumar ◽  
Ramaswamy Malathi
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Klebsiella Pneumonia ◽  
Coleus Forskohlii ◽  
Ms Analysis ◽  
Phytochemical Investigation

<p class="Abstract">The aim of this study was to investigate the phytochemical constituents, gas chromatography-mass spectrometry (GC-MS) analysis and antimicrobial activity of <em>Coleus forskohlii</em>. The different solvents such as ethanol, chloroform, acetone and aqueous extracts were identified pharmacologically as important bioactive compounds and their antimicrobial properties were studied. In the phytochemical investigation almost all the ethanol extract of leaf, stem and root having secondary metabolites like alkaloids, flavonoids, tannins, saponins, terpenoids, and steroids. The active constituents of the ethanol extract of <em>C. forskohlii</em> root was studied by GC-MS analysis. According to the antimicrobial results ethanol extract of <em>C. froshkolii</em> root showed highest antibacterial activity compared with stem and leaf. The highest antimicrobial activity was observed against <em>Klebsiella pneumonia</em> (19 mm) and <em>Candida albicans </em>(16 mm) in ethanol extract of root. Among the above extracts of leaf, stem and root, ethanol extract of root having antimicrobial activities due to the presence of phytoconstituents.</p><p class="FigureLegend"> </p>

scholarly journals EVALUATION OF AQUEOUS AND ETHANOLIC EXTRACTS OF SYZYGIUM CARYOPHYLLATUM FOR ANTIBACTERIAL ACTIVITIES

2018 ◽  
Vol 11 (2) ◽  
pp. 235
Author(s):  
Raghunath Pendru ◽  
Jagan Nadipelly ◽  
Jyothinath Kothapalli ◽  
Subbannayya Kotigadde
Keyword(s):  
Antimicrobial Activity ◽  
Aqueous Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

 Objective: It has been well documented that traditional medicinal plants confer considerable antimicrobial activity against various microorganisms.Methods: The present study was designed to evaluate the antimicrobial properties of aqueous and ethanolic extracts of the leaves of Syzygium caryophyllatum against Staphylococcus aureus, Escherichia coli, Salmonella typhi, Shigella flexneri, and Vibrio cholerae. The aqueous extract was prepared using cold percolation method, and ethanolic extract was prepared in Soxhlet apparatus using ethyl alcohol. Antimicrobial activity is being determined by time-kill assay and minimum inhibitory concentration.Results: From our study, it is found that all tested organisms were sensitive to both aqueous and ethanolic extract of leaves of S. caryophyllatum. The ethanolic extract showed better antibacterial activity against S. aureus and S. typhi when compare to aqueous extract. The phytochemical analysis confirmed the presence of flavonoids, alkaloids, glycosides, steroids, phenols, tannins, and saponins in the ethanolic and aqueous extract of S. caryophyllatum. Conclusion: We hypothesize that these active phytoconstitutents might be responsible for the antimicrobial activity of this plant.

scholarly journals Antimicrobial Activity of the Essential Oil Obtained from the Seed and Oleo-Gum-Resin of Ferula assa-foetida Against Oral Pathogens

2019 ◽  
Author(s):  
Alireza Daneshkazemi ◽  
Hengameh Zandi ◽  
Abdolrahim Davari ◽  
Mahmood Vakili ◽  
Majid Emtiazi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Streptococcus Sobrinus ◽  
Disk Diffusion Method

Objectives: The objective of this study was to evaluate the antimicrobial activity of the essential oil obtained from oleo-gum-resin and seeds of Ferula assa-foetida. Materials and Methods: Ferula assa-foetida plants were collected from Tabas, Yazd Province, Iran, during summer 2017. Then, essential oils were obtained from its seeds and oleo-gum-resin using hydrodistillation. Gas chromatography-mass spectrometry (GC-MS) test was performed to determine the contents of the essential oils. Four different concentrations of each oil were prepared (2.5, 5, 10, and 20 μg/ml), and the antimicrobial activity of each dose against four oral bacteria (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, Streptococcus salivarius, and Lactobacillus rhamnosus) was evaluated using the disk diffusion method. The data were analyzed using analysis of variance (ANOVA) and Kruskal-Wallis test in SPSS 17 software. Results: The GC-MS findings exhibited that the main compounds found in essential oils yielded from the seeds and oleo gum resin were (Z) -1-propenyl sec-butyl disulfide and (E) -1-propenyl sec-butyl disulfide. Ferula assa-foetida plant showed a significant antimicrobial effect (P<0.05). The essential oil from Ferula assa-foetida oleo-gum-resin had significantly stronger antibacterial properties compared to the essential oil from Ferula assa-foetida seeds (P<0.001). Both essential oils showed antibacterial properties similar to that of Chlorhexidine. The growth inhibition zone was significantly dependent on the essential oil concentration for all bacteria (P<0.05). Conclusion: Our study revealed that essential oils from seeds and oleo-gum-resin of Ferula assa-foetida have antimicrobial properties. More laboratory studies are required to reach a definitive conclusion.

scholarly journals Evaluation of the Effectiveness of Nine Brands of Toothpaste on Streptococcus mutans Isolated from the Mouth of Students in Veritas University, Abuja

2020 ◽  
pp. 36-41
Author(s):  
T. O. Ozoude ◽  
T. C. Okolie ◽  
O. E. Effiom
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Streptococcus Mutans ◽  
Bacterial Load ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Dental Hard Tissues ◽  
Zones Of Inhibition

Dental caries is an infectious microbiological disease of the teeth that end up in the destruction of dental hard tissues. The use of toothpaste has been used to reduce the bacterial load in the mouth because they contain antimicrobial properties such as triclosan, fluoride, and so on. The study aimed to determine the effect of nine brands of toothpaste on Streptococcus mutans isolated from the mouth of students in veritas university. Following the isolation of Streptococcus mutans from samples collected from the mouths of Veritas students’, the antimicrobial activity of different concentrations of the dentifrices was determined by modified agar well diffusion method. In this method, Muller Hinton agar plates were seeded with a loop full of 24-hour cultures of the bacterial isolate. A sterile 8 mm cork-borer was used to cut one central and five wells at equidistance in each of the plates. 0.3 ml of the dentifrice dilutions was introduced into each of the five wells while the same amount of sterile distilled water was introduced into the first well as control. The plates were incubated at 37°C for 24 hours. The antimicrobial activity was evaluated by measuring the diameter of zones of inhibition (in mm). The diameter in which the bacteria were inhibited was indicative of the toothpaste’s antibacterial potential.  The zones of inhibition on the growth of the test strain were defined by the location where visible growth had been inhibited. All the plates were made in triplicates and the experiments repeated twice. The result showed that Mentadent-P was the most effective dentifrice with an inhibition zone of 2.8 mm, Pepsodent had inhibition zone of 2.6 mm, Oral B with 2.3 mm, Close up with 2.2 mm, followed by Colgate with a diameter of 1.9 mm, Special Lucky with a diameter of 1.6 mm, Darbur Promise with a diameter of 1.6 mm, Maclean’s with a zone of inhibition of 1.5 mm, and MY- MY with the least effective toothpaste with a zone of inhibition of 1.0 mm in diameter. The results also showed that toothpaste containing fluoride as their main antimicrobial agent proved more effective against streptococcusmutans than other toothpaste not containing fluoride as their main antimicrobial agent.

scholarly journals Evaluation of Extracts of Leaves of Crinum jagus for Antimicrobial Properties

2020 ◽  
Vol 24 (7) ◽  
pp. 1197-1201
Author(s):  
T.T. Alawode ◽  
L. Lajide ◽  
B.J. Owolabi ◽  
M.T. Olaleye
Keyword(s):  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Penicillium Notatum ◽  
E Coli ◽  
Agar Diffusion ◽  
Zones Of Inhibition

Plants have been used in ethno-medicine for ages in the treatment of various diseases. In the current study, the leaves of C. jagus are investigated  for antimicrobial activities. The leaves were dried and extracted successively with hexane, ethylacetate and methanol. The concentrated extracts  were screened for activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebisidlae pneumonae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer at concentrations between 6.25 and 200 mg/ml using the agar diffusion method. The Minimum Inhibitory Concentration (MIC) was also determined. The percentage yields obtained were 0.92 %, 1.20 %  and 25.2 % for the hexane, ethylacetate and methanol extracts respectively. The zones of inhibition of the organisms by the extracts generally increased with the concentrations. The methanol extract showed the best activity of the three extracts tested. The methanol extract had values ranging between 20 mm and 26 mm against the bacteria at 200 mg/ml. This extract also showed values between 18 mm and 20 mm against the  fungi at 200 mg/ml. The lowest MICs values (of 2.5 mg/ml) were obtained against S. aureus, E. coli, B. subtilis and S. typhi. The polar constituents in  the leaves of the plant are likely responsible for the antimicrobial properties observed. None of the extracts showed activity comparable to those of the standard drugs, however the methanol extract showed some potential as a source of antimicrobial compounds. Keywords: Crinum jagus, antimicrobial, Agar diffusion, MIC

Sign in / Sign up

Export Citation Format

Share Document