scholarly journals STANDARDIZATION OF EFFECTIVE DOSE OF FOWL CHOLERA VACCINE IN PIGEON IN BANGLADESH

2018 ◽  
Vol 15 (2) ◽  
pp. 97-105
Author(s):  
M. T. Islam ◽  
M. H. Ali ◽  
A. Chandra ◽  
S. Saha ◽  
M. A. Islam
Keyword(s):  
Effective Dose ◽  
Antibody Titer ◽  
Pasteurella Multocida ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Serotype 1 ◽  
Group A ◽  
The Mean ◽  
And Control ◽  
Different Doses

An experiment was conducted to determine the effective dose of formalin killed (FK) fowl cholera (FC) vaccines prepared with virulent avian Pasteurella multocida (PM 38) serotype 1 (X-73) collected from the laboratory of the Department of Microbiology and Hygiene, BAU, Mymensingh. To determine the effective dose of vaccine, 7 weeks old 30 pigeons were immunized and each group consists of 5 birds. The groups are represented by A, B, C, D, E and F. The birds belonging to groups (A-E) were vaccinated with different doses of vaccine, after two weeks of first, second immunization and challenge experiment, blood was collected from all vaccinated birds, and serum was analyzed to determine antibody titer against P. multocida by passive hemagglutination test (PHA). The PHA titer after two weeks of first vaccination were 16±3.92, 17.6±3.92, 25.6±3.92, 32±8.76, 35.2±7.84 of group A,B,C,D and E, respectively at the dose of 0.2ml (0.26×108 CFU)/birds, 0.4ml (0.5×108 CFU)/birds, 0.8 ml (1.04×108 CFU)/birds, 1ml (1.3×108 CFU)/birds, respectively. The PHA titer of prevaccination and control birds was <4. The PHA titer after 2 weeks of second vaccination or boostering were 32±8.76, 35.2±7.84, 44.8±7.84, 57.6±18.66, 70.4±15.68, of group A,B,C,D and E, respectively. After 2 weeks of challenge infection, the mean PHA titer were 44.8±7.84, 51.2±7.84, 70.4±15.68, 102.4±15.68 and 140.8±31.34 of group A,B,C,D and E, respectively. In this experiment, the antibody titer of the vaccinated pigeons with 0.4, 0.6, 0.8 and 1ml per bird via intramuscular route were higher than that of the pigeons vaccinated with 0.4ml/bird, 0.6ml/bird, 0.8ml/bird and 1ml/bird were satisfactory in terms of protective potential against P. multocida. For prevention and control of avian pasteurellosis 0.4ml to o.6ml (0.52×108 CFU to 0.78×108 CFU)/birds of vaccine may be used instead of 1ml (1.3×108 CFU)/birds for better immunization of pigeon against fowl cholera infection.

scholarly journals Isolation and Identification of Pasteurella multocida from Chicken for the Preparation of Oil Adjuvanted Vaccine

2013 ◽  
Vol 2 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Samina Ievy ◽  
Mohammad Ferdousur Rahman Khan ◽  
Md Ariful Islam ◽  
Md Bahanur Rahman
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Challenge Infection ◽  
Cholera Vaccine ◽  
Isolation And Identification ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Adjuvanted Vaccine ◽  
Group A ◽  
The Mean

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

scholarly journals Comparative efficacy of BAU-fowl cholera and DLS-fowl cholera vaccines in indigenous chicken

2018 ◽  
Vol 5 (2) ◽  
pp. 193-199
Author(s):  
Chamak Nahar Shampa ◽  
Suma Akter ◽  
Sukumar Saha ◽  
Md Hadiuzzaman ◽  
Azhar Ul Alam ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Research Work ◽  
Booster Vaccination ◽  
Indigenous Chicken ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Unvaccinated Control ◽  
Group A ◽  
The Mean ◽  
Group B

The present study was conducted to determine the immune response induced in indigenous chicken produced against BAU-FC and DLS-FC vaccines with their efficacy study against Pasteurella multocida. A total of forty (40) chickens were selected and divided into Group A (15), Group B (15) and Group C (10). Group A and B were vaccinated with BAU-FCV and DLS-FCV, respectively at the dose rate of 0.5 ml through SC at six weeks of age followed by boostering at 10 weeks of age while Group C was kept as unvaccinated control. Sera samples were collected after primary and booster vaccination and antibody titre was determined by Passive hemagglutination (PHA) test. The mean PHA titres recorded at 4 weeks after primary vaccination was 51.20 ± 7.84 in birds of group A and 38.40 ± 6.40 in birds of Group B. After booster vaccination, mean PHA titer was found 140.80 ± 31.35 at 16 weeks of age in case of BAU-FC vaccinated group and 115.20 ± 12.80 in case of DLS-FC vaccinated group. The mean PHA titer was 204.80 ± 31.35 and 179.20 ± 31.35 at 19 weeks of age in birds of BAU-FC and DLS-FC vaccinated group, respectively. Birds of all groups were challenged with virulent P. multocida at 17 weeks of age. It was observed that vaccinated chickens showed maximal resistance (100%) following challenge with virulent whereas unvaccinated control birds failed to resist the challenge infection. It can be assumed from the findings of present research work that both BAU-FCV and DLS-FCV are able to protect indigenous chicken from the outbreak of avian pasteurellosis and BAU-FV vaccine showed relatively higher immuno-protective titre than that of DLS-FC vaccine.Res. Agric., Livest. Fish.5(2): 193-199, August 2018

scholarly journals THE EFFICACY OF VACCINATION OF LAYER CHICKENS WITH INACTIVATED FOWL CHOLERA BACTERIN PREPARED FROM LOCAL EGYPTIAN STRAINS OF Pasteurella multocida

2020 ◽  
Vol 57 (4) ◽  
Author(s):  
Wafaa Abd El-Ghany ◽  
Hanan Ali Ahmed ◽  
Ali Zaher Qandoos ◽  
Mohamed Abd El-Rahman Bosila Bosila
Keyword(s):  
Pasteurella Multocida ◽  
Histopathological Examination ◽  
Clinical Signs ◽  
Post Vaccination ◽  
Fowl Cholera ◽  
Humoral Immune ◽  
Group A ◽  
The Mean ◽  
Layer Chickens ◽  
Group B

This study was carried out to evaluate the efficacy of vaccination of layer chickens with inactivated FC bacterin prepared from local Egyptian strains of Pasteurella multocida (P. multocida). A total of 200 layer chickens were divided into 5 equal groups, 40 for each. At the age of 6 weeks, chickens in groups (A) and (B) were vaccinated with P. multocida serotypes A:1 and A:3, respectively, booster doses were given after 3 weeks (9 weeks old) and challenge was done with virulent serotypes A:1 and A:3 at 2 weeks later (11 weeks old). Chickens in groups (C) and (D) were not vaccinated, only challenged with P. multocida serotype A:1 and A:3, respectively. Birds in group (E) were kept as non-vaccinated and non-challenged. Blood samples were collected weekly from all groups for humoral immune response. All the birds were kept under observation for signs, mortalities, lesions and re-isolation of challenging organism and for histopathological examination. Results of the mean Enzyme Linked Immuno-Sorbent Assay (ELISA) revealed that the highest level was at 5 weeks post vaccination as the titers reached to 3970 in group (A) and 3905 in group (B). The clinical signs, mortality rate and lesions were mild in the vaccinated birds while severe lesions were in non-vaccinated and challenged birds. The protection rates were 85 % and 80 % in groups (A) and (B); respectively, while 10 % and 20 % in groups (C) and (D); respectively. The re-isolation rates of P. multocida after challenge were 95 % and 90 % in non-vaccinated-challenged birds with P. multocida serotypes A:1 and A:3; respectively, while they were 25 % and 15 % in vaccinated-challenged groups with P. multocida serotypes A:1 and A:3; respectively. Histopathological examination of P. multocida vaccinated-challenged birds revealed mild to no microscopic lesions when compared with non-vaccinated challenged chickens. In conclusion, the prepared FC inactivated bacterin from the local Egyptian predominant P. multocida serovars proved efficacy and protection of layer chickens. Key words: Pasteurella multocida; chickens; immunization; protection; Egypt UČINKOVITOST CEPLJENJA KOKOŠI NESNIC Z INAKTIVIRANO BAKTERIJO KOLERE PERJADI, PRIPRAVLJENE IZ LOKALNIH EGIPTOVSKIH SEVOV BAKTERIJE Pasteurella multocida Povzetek: Raziskava je bila izvedena z namenom ocenitve učinkovitosti cepljenja kokoši nesnic z inaktivirano bakterijo FC, pripravljeno iz lokalnih egiptovskih sevov bakterije Pasteurella multocida (P. multocida). Skupno 200 kokoši nesnic je bilo razdeljenih v 5 enakih skupin. V vsaki skupini je bilo 40 kokoši. Pri 6 tednih smo kokoši v skupinah A in B cepili s serotipoma P. multocida A:1 in A:3, po 3 tednih, ko so bile živali stare 9 tednov, so dobile poživitvene doze cepiva. Po dveh tednih (v starosti 11 tednov) so bile kokoši okužene z virulentnima serotipoma A:1 in A:3. Piščanci v skupinah C in D niso bili cepljeni temveč samo okuženi s serotipoma A:1 in A:3. Kokoši v skupini E niso bile niti cepljene, niti okužene. Vzorci krvi so bili odvzeti pri vseh skupinah tedensko za preverjanje humoralnega imunskega odziva. Vse kokoši smo stalno opazovali in beležili prisotnost bolezenskih znakov, različnih ran in umiranje kokoši. Pri poginulih kokoših smo osamili bakterije ter opravili histopatološki pregled. Rezultati encimsko-imunskega testa (ELISA) so pokazali da je bila najvišja stopnja zaščite dosežena 5 tednov po cepljenju, saj so titri dosegli 3970 v skupini A in 3905 v skupini B. Klinični znaki, stopnja umrljivosti in rane so bili pri cepljenih kokoših blagi, hude rane pa so bile vidne pri necepljenih in okuženih kokoših. Stopnja zaščite je bila v skupinah A in B 85- oziroma 80-odstotna, v skupinah C in D pa 10- oziroma 20-odstotna. Stopnje ponovne izolacije P. multocida po okužbi so bile 90 in 95 odstotkov pri kokoših, ki niso bile cepljene, medtem, ko so bile v skupinah, ki so bile okužene s P. multocida serotipa A:1 in A:3 15- in 25-odstotkov. Histopatološki pregled cepljenih in okuženih kokoši je pokazal popolno odsotnost ali prisotnost blagih mikroskopskih poškodb, medtem ko so imele necepljene okužene kokoši bolj obsežne histopatološke poškodbe. Pripravljena inaktivirana bakterija FC iz lokalnih egiptovskih prevladujočih serovarov P. multocide se je izkazala za učinkovito zaščito kokoši nesnic.Ključne besede: Pasteurella multocida; kokoši; imunizacija; zaščita; Egipt

scholarly journals Immunogenicity of capsular extract prepared from a local duck isolate of Pasteurella multocida

1970 ◽  
Vol 6 (1) ◽  
pp. 19-22
Author(s):  
M Sukul ◽  
MSR Khan ◽  
MT Rahman ◽  
K Begum
Keyword(s):  
Pasteurella Multocida ◽  
Laboratory Animals ◽  
Serological Response ◽  
Cholera Vaccine ◽  
Serum Samples ◽  
Passive Hemagglutination ◽  
Fowl Cholera ◽  
Capsular Antigen ◽  
Group A ◽  
Group B

The immunogenic response and protection of duck with capsular extract of local isolate of duck cholera organism, i.e. Pasteurella multocida was compared with that of conventional fowl cholera vaccine prepared in Bangladesh Agricultural University, Mymensingh. Feces, liver, heart, lungs, trachea and intestinal swabs were collected from a total of 50 sick and apparently healthy ducks. The duck cholera organisms were isolated and characterized by their cultural, physiochemical, staining properties and laboratory animals and also duck and ducklings inoculation. Capsular antigen was prepared from this isolate. Twelve weeks aged ducks of Jinding breeds were divided into three groups such as A, B and C. Each duck of group A was inoculated with 1 ml of capsular antigen containing 200 µl capsular protein and 1 ml of Bangladesh Agricultural University fowl cholera vaccine in group B intramuscularly followed by second vaccination with same vaccine with similar dose and route at 15 days interval in group A and B respectively. Ducks of group C were kept as control. Sera samples of each of the ducks of all the three groups were collected at 7 and 14 days post-vaccination following each of the primary and secondary vaccination. Each of the serum samples of all the three groups of birds was titrated by using passive hemagglutination (PHA) test and results recorded that both the capsular extract (96.00 ± 45.25) and fowl cholera vaccine (108 ± 89.37) produced more or less similar serological response at two weeks after secondary vaccination. Protection test was performed with all birds of three groups after 21 days of secondary vaccination and the results showed that ducks of both group A and B conferred 100% protection. Key words: Pasteurella multocida, capsular extract, fowl cholera vaccine, duck, immunogenicity DOI = 10.3329/bjvm.v6i1.1333 Bangl. J. Vet. Med. (2008). 6 (1): 19-22

scholarly journals Comparative efficacy of BAU-Fowl cholera and DLS-Fowl cholera vaccines in indigenous ducks

2019 ◽  
Author(s):  
S. Akter ◽  
C. N. Shampa ◽  
M. A. Islam ◽  
A. U. Alam ◽  
M. Hadiuzzaman ◽  
...  
Keyword(s):  
Pasteurella Multocida ◽  
Booster Vaccination ◽  
Significant Loss ◽  
Challenge Infection ◽  
Fowl Cholera ◽  
Antibody Titres ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Cholera Vaccines

Background: Duck cholera is an acute, fatal, septicemic disease of domestic ducks which is responsible for significant loss in duck population. The present study was conducted to compare the immunogenicity of two formalin killed fowl cholera vaccines (BAU-FCV and DLS-FCV) in indigenous ducks. Methods: The experimental ducks were divided into three groups (A=15, B=15 and C =10 ducks) of which birds of Group A and Group B were inoculated with 0.5 ml of BAU-FCV and DLS-FCV, respectively through subcutaneous route at the age of 10 weeks whereas ducks of group C were kept as unvaccinated control. Booster vaccination was done with same dose and route at 14 weeks of age. Challenge infection was conducted after 2 weeks of booster vaccination. Results: The mean PHA antibody titres on 15 days post vaccination (DPPV), 28 DPPV, 15 days postsecondary vaccination (DPSV), 28 DPSV and 15 days post challenge were 25.60 ± 3.92, 51.20 ± 7.84, 89.60 ± 15.68, 166.40 ± 38.40 and 204.80 ± 31.35, respectively in ducks of Group A whereas, the mean antibody titres in ducks of Group B were 25.60 ± 3.92, 44.80 ± 7.84, 64.00 ± 7.53,102.40 ± 15.68 and 179.20 ± 31.35 at 15 DPPV, 28 DPPV, 15 DPSV, 28 DPSV and 15 days after challenge, respectively. In this investigation, slightly higher immune responses were observed in ducks of Group A vaccinated with BAU-FCV compare to ducks of Group B vaccinated with DLS-FCV. Birds of both vaccinated groups conferred 100% protection against challenge infection with virulent Pasteurella multocida whereas, 100% mortality was observed in control ducks after challenge. Conclusion: Both vaccines were found to be safe and effective for the vaccination of indigenous ducks against duck cholera.

scholarly journals Trends in the doses of the population of the Russian Federation in 2003–2018

2020 ◽  
Vol 12 (4) ◽  
pp. 96-122 ◽  
Author(s):  
Anatoly N. Barkovsky ◽  
Anzhelika A. Bratilova ◽  
Tatyana A. Kormanovskaya ◽  
Ruslan R. Akhmatdinov ◽  
Rustam R. Akhmatdinov
Keyword(s):  
Effective Dose ◽  
Russian Federation ◽  
Radiation Safety ◽  
Information Bulletin ◽  
Group A ◽  
Effective Doses ◽  
The Russian Federation ◽  
Medical Exposure ◽  
The Mean ◽  
Group B

In the last years radiation-hygienic passportization and Joint state system of control and accounting of the individual doses of the citizens have become a trusted source of the validated data on the state of the radiation safety in the Russian Federation and have become the basis of the Russian system of the information provision of the radiation safety. Annual analysis of that data is presented in the annual information bulletin “Doses of the population of the Russian Federation”. The current study is focused on the analysis of the data from the information bulletins to evaluate the trends in the doses of the Russian population from main sources of ionizing exposure in 2003–2018. It is indicated that the mean annual doses from man-made exposure of the group A staff in that period varies from 1,0–1,4 mSv per year, group B – 0,65–0,8 mSv per year. The number of exceedances of the annual dose limits from group A staff varied from 5 to 52 per year; for group B – 0–22 per year. For the whole investigated period there were only three cases of the exceedance of the 50 mSv annual individual dose for group A staff and four cases of the exceedance of the 12,5 mSv annual effective dose for group B staff. Mean effective dose per X-ray examination in 2006–2013 was decreasing from 0,56 mSv to 0,26 mSv and started to slowly increase leading to 0,29 mSv in 2018. The same trends were identified for the mean dose from medical exposure per citizen: reduction in 2003-2013 from 0,86 mSv to 0,45 mSv with the subsequent increase up to 0,56 mSv. The biggest contributor for the dose from medical exposure is computed tomography: the number of CT examinations increased from 1,3 mln in 2003 to 11,7 mln in 2018; contribution to the collective dose – from 5,7% to 54%. Mean annual effective doses from natural exposure of the population of the Russian regions varied from 1,6 to 15,1 mSv per year with the mean value for Russia of 3,11 – 3,79 mSv/year. The highest mean annual doses exceeding 5 mSv in each year were observed in Republic of Altay, Jewish Independent Region, Stavropol and Zabaikalskiy Krai, Republic of Tyva and Irkutsk region. Total effective doses exceeding 10 mSv/year were observed in Republic of Altay and Jewish Independent Region. For ten subjects of the Russain Federation (Republics of Buryatia, Altay, Tyva, Stavropol and Zabaikalskiy Krai, Irkutsk and Chita regions, Jewish Independent Region, Aginsko-Buryatskiy and Ust’-Ordinskiy independent districts) they exceed 5 mSv/year for the whole assessed period.

scholarly journals Serum Periostin Level in Asthmatic Children with Exercise Induced Bronchospasm

2020 ◽  
pp. 53-67
Author(s):  
Reem M. Soliman ◽  
Mohamed B. Hamza ◽  
Rasha M. El-Shafiey ◽  
Hesham A. Elserogy ◽  
Nabil M El-Esawy
Keyword(s):  
Test Group ◽  
Mean Value ◽  
Control Group ◽  
Asthmatic Children ◽  
Group A ◽  
The Mean ◽  
Exercise Induced ◽  
Group B ◽  
And Control ◽  
Serum Periostin

Background: There are few biomarkers that can be easily accessed in clinical settings and may reflect refractory Th2-eosinophlic inflammation and remodeling of the asthmatic airways. Serum periostin may be one such biomarker to aid our understanding of the patho-bio-physiology of asthma and exercise induced asthma. The aim of the study is to explore the relationship between serum periostin level and exercise induced bronchoconstriction in asthmatic children. Materials and Methods: This cross-sectional study was carried out on (90) children both sexes aged from 6 to 15 years including, (60) children with bronchial asthma and (30) children were enrolled as control group in the period from January 2018 to January 2019. Patients were randomly classified into two groups: I) Patient group: divided into 2 groups according to standardized treadmill exercise challenge test: Group A: (30) asthmatic children with positive test. Group B: (30) asthmatic children with negative test. II-Control group: (30) children apparently healthy with no personal or family history of asthma. All children were subjected to the following Investigations: Chest x-ray, pulmonary functions tests (FEV1 & PEFR) except controls, Laboratory investigations as CBC and Serum periostin level. Results: The mean values of both the percentage of PEFR and FEV1 after exercise in group A were significantly lower than those in group B and the percentage of PEFR and FEV1 after exercise in each group were significantly lower than the percentage before exercise in the same group. The mean value of eosinophilic count in group A was significantly higher than (group B and control group) and the mean value of eosinophilic count in group B was significantly higher than control group. The mean value of serum level of periostin in group A was significantly higher than (group B and control group), however, there was no significant difference between group B and control group as regard to serum level of periostin. Chest tightness, cough and wheezes after exercise and eosinophilic count in patients with high serum periostin level were significantly higher than patients with low serum periostin level, and both PEFR and FEV1 after exercise in patients with high serum periostin level were significantly lower than patients with low serum periostin level. Also the normal serum periostin levels vary among different age groups. Conclusion: Serum periostin level can be considered as a useful biomarker for diagnosis of Exercise induced bronchospasm (EIB) in asthmatic children especially when lung function test cannot be done However, cautious is required in evaluating serum periostin levels in children because it varies with age.

The Impact of Virtual Laboratories on Academic Achievement and Learning Motivation in the Students of Sudanese Secondary School

1970 ◽  
Vol 4 (9) ◽  
pp. 20
Author(s):  
Dr. Esam Edris Kamtor Al Hassan
Keyword(s):  
Secondary School ◽  
Learning Motivation ◽  
Control Group ◽  
Virtual Laboratories ◽  
Post Test ◽  
Group A ◽  
The Mean ◽  
And Control ◽  
The Impact ◽  
Experimental Group

This study aimed to investigate the impact of virtual laboratories (VLabs) on academic achievement and Learning Motivation in the Students of Sudanese Secondary School in the subject of chemistry. The study followed a quasi-experimental method. The population consisted of second-grade students of Almutamaar school boys in Omdurman, the study sample was selected randomly totaling 54 students were divided randomly into two groups: 27 for experimental group and 27 for control group. The study used achievement pre & post tests, and Learning Motivation scale as tools for data collection. The result showed that  there  were statistically significant differences at the level (0.05) between the mean scores of students of the experimental group (a group is taught by VLabs) and control group (a group is taught by conventional method) in the post test in favor of the experimental group, and there were statistically significant differences at the level (0.05) between the mean scores of pre and post test for the students of the experimental group in favor of the post test, and there were statistically significant differences at the level (0.05) in the Learning Motivation scale between the experimental group( taught by VLabs) and control group (taught by conventional method)  for the favor of the experimental group too.

scholarly journals Comparative immunogenicity of fowl cholera vaccine in Jinding ducks

2014 ◽  
Vol 30 (2) ◽  
pp. 41-45 ◽  
Author(s):  
S Sultana ◽  
S Saha ◽  
MM Amin
Keyword(s):  
Pasteurella Multocida ◽  
Booster Vaccination ◽  
Primary Vaccination ◽  
Challenge Infection ◽  
Fowl Cholera ◽  
Significant Difference ◽  
Unvaccinated Control ◽  
Antibody Titres ◽  
Group A ◽  
Group B

This study compared the immunogenicity of alum-precipitated formalin-killed fowl cholera vaccines (BAU-FCV and LRI-FCV) in Jinding ducks. The ducks were divided into three groups (A = 14, B = 14, C = 12). Group A was inoculated with BAU- FCV 0.5 mL and group B with LRI- FCV 1.0 mL intramuscularly (im) at the age of six weeks and group C served as unvaccinated control. Booster vaccination was administered similarly at 11 weeks of age in groups A and B. Challenge infection was given to all birds two weeks after booster vaccination. Passive Haemagglutination Assay (PHA) antibody titres in group A were 59.4 ± 4.6 21 days after primary vaccination, 137.1 ± 21.8 15 days after booster vaccination, 100.6 ± 12.9 21 days after booster vaccination, and 256.0 ± 48.4 15 days after challenge. In group B, titres were 50.3 ± 6.5, 118.9 ± 9.1, 91.4 ± 12.9, 237.7 ± 51.7, respectively, whereas titres in group C remained at ?4.0 ± 0.0. The antibody titres were insignificant when compared between pre-vaccination and 21 days after primary vaccination in both vaccinated groups (A and B). PHA antibody titres of groups A were significantly (P < 0.0001) increased at 15 days after booster and in case of group B the antibody titres were insignificant. At 15 days after challenge the antibody titres were highly significant in both groups (A and B). There was no significant difference between the two vaccinated groups. Following challenge infection with virulent Pasteurella multocida 88.9% of birds vaccinated with BAU-FCV, and 77.8% of birds vaccinated with LRI-FCV survived, while all unvaccinated birds died. Both vaccines were safe and effective. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18253 Bangl. vet. 2013. Vol. 30, No. 2, 41-45

scholarly journals The Effects of Topical Olive Oil on the Pain Intensity among Patients with Knee Osteoarthritis

2021 ◽  
pp. 33-38
Author(s):  
Abas Ghaysouri ◽  
Golbahar Iri ◽  
Amirhosein Meisami ◽  
Milad Borji ◽  
Behrouz Soltany
Keyword(s):  
Knee Osteoarthritis ◽  
Pain Intensity ◽  
Olive Oil ◽  
Control Group ◽  
Control Groups ◽  
Group A ◽  
The Mean ◽  
And Control ◽  
Experimental Group ◽  
Scale Data

Given the importance of relieving pain and disability experienced by the patients, the present study aimed to study the Effects of topical olive oil on the pain intensity among patients with knee osteoarthritis. The patients were randomly assigned into the experimental (28 patients) and control groups (27 patients). Firstly, in tropical olive oil (TOO) group, a high-quality olive oil, branded as Famila was used and in the control group, Diclofenac gel was applied. In experimental groups, 5 drops of herbal oils were applied to the knee thrice a day at morning, noon and evening and the knees were covered with a nylon sheet for more absorption. The patients were followed up for 4 weeks and their capability and pain intensities were evaluated prior the study, during the first week, the second week and the fourth week with Visual Analogue Scale. Data were analyzed using descriptive and analytical tests by SPSS V. 16 software. According to the findings, also the Mean (SD) pre-intervention Pain score in experimental group A was 8.10(0.91), which decreased to 1.78(0.78) after the intervention and in the control group from 8.55(0.69) to 3.85(0.98). Due to the greater effect of olive oil compared to Diclofenac gel on reducing pain of patients with KOA, it is recommended that patients use olive oil. Further studies are also recommended.

Sign in / Sign up

Export Citation Format

Share Document