scholarly journals Seroprevalence of Mycoplasma gallisepticum infection in chicken in the greater Rajshahi district of Bangladesh

Author(s):  
KMM Hossain ◽  
MY Ali ◽  
MI Haque

A serological investigation of Mycoplasma gallisepticum (MG) infection in chicken was conducted in the greater Rajshahi district of Bangladesh on 115 flocks during the period from July 2006 to June 2007. A total of 575 sera samples were collected and tested by serum plate agglutination (SPA) test using Mycoplasma gallisepticum antigen (Nobilis MG, Intervet International B.V. Boxmeer-Holland) to determine specific antibodies in different flocks. The overall seroprevalence of MG infection in different flocks was recorded as 55.13%. Seroprevalence of MG infection was found significantly (p < 0.05) higher during winter season (61.48%) than in summer (47.74%). Again this was recorded in different age groups, with significantly (p < 0.01) higher occurrence in young (72.72%) compared to adult (44.00%). On the other hand, the seroprevalence of MG infection was found little (p>0.05) higher in large flocks (62.86%) in comparison to small flocks (52.00%). It has been found that MG infection is still an important disease problem in chickens in Bangladesh. Key words: Seroprevalence, Mycoplasma gallisepticum, chicken, serum plate agglutination (SPA) test DOI = 10.3329/bjvm.v5i1.1302 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 09-14

2012 ◽  
Vol 8 (2) ◽  
pp. 93-96 ◽  
Author(s):  
MA Jalil ◽  
MT Islam

A cross-sectional study was carried out on 96 commercial layer farms of six upazilas of Khulna district to know the seroprevalence of Mycoplasma gallisepticum (MG) infection during the period from August 2009 to July 2010. A total of 1268 sera samples were collected from randomly selected layer birds of different age groups and tested by serum plate agglutination (SPA) test by using commercial MG antigen (Nobilis® MG, Intervet International) to detect the presence of antibodies against MG. The overall prevalence of MG infection was recorded as 67.4%. Age-wise analysis revealed that there was significant relationship (p<0.001) between MG infection and age of birds. The highest prevalence of MG infection was 71.2% in 21-56 weeks age group followed by 66.8% in 8-20 weeks age group. There was significant (p<0.001) variation in the seroprevalence in terms of areas of study (upazilas). Significant (p<0.001) effect of seasons in the seroprevalence of MG infection in layer birds was observed in the present study. The prevalence of MG infection was the highest (75.6%) in winter season followed by summer (66.3%) and rainy (60.8%) seasons. The seroprevalence of MG infection was higher in most of the large flocks and lower in small flocks with some exceptions. It is evident from the present study that above 50% layer birds are infected with MG organism. Therefore, it may be suggested that the layer farms should be routinely checked to monitor MG infection and the reactor birds should be culled since MG organism has the potential to transmit vertically.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.9620Bangl. J. Vet. Med. (2010). 8 (2) : 93-96 


2021 ◽  
Vol 3 (4) ◽  
pp. 31-36
Author(s):  
Aurangazeb Kabir ◽  
Md. Obaidullah Al Masum ◽  
Most. Sabina Yasmin ◽  
Md. Golam Sarwar ◽  
Md. Shamim Hossain ◽  
...  

Mycoplasma gallisepticum (MG) infection is very common in birds that cause respiratory infections in chickens, turkeys, and other avian species. It has brought about a considerable amount of financial losses to the poultry sector in Bangladesh. We conducted a study on the Seroprevalence of MG infection in two different geographical areas of Bangladesh under Rajshahi district, namely Paba and Bagmaraupazila. 800 sera samples were collected and tested with Rapid Serum Plate Agglutination Test (RSA) to identify the MG antibodies using commercial Mycoplasma gallisepticum antigen. The gross Seroprevalence of MG infection was 59.25% in the study area. The maximum rate (68.80%) of infection was found in the winter season, followed by the summer season (49.36%). The result further revealed that the condition was higher (69.01%) in larger-sized flocks than in small (53.63%). We noticed that younger birds having 10-20 weeks of age are more prone to be affected with avian mycoplamosis with an infection rate of 72% compared to their adult counterpart of 71+ weeks with 52% morbidity. Our study revealed that Mycoplasma gallisepticum infection is prevalent in Paba and Bagmaraupazila in Rajshahi. The farms should take strict bio-security measures to mitigate this infection in the mentioned areas. Proper medications for the affected birds and timely prophylactic measures for the healthy ones could be practical and preventive strategies against avian mycoplasmosis. Amid limitations, we conducted our experiments, and thus further research is warranted to substantially assess and validates our observations.


2021 ◽  
Vol 7 (3) ◽  
pp. 292-297
Author(s):  
Md Shahjamal Hossain Sobuj ◽  
Bidyut Matubber ◽  
Md Sahidul Islam ◽  
Md Shamsur Rahman Sumon ◽  
Mohammed Lalmoddin Mollah ◽  
...  

Mycoplasma gallisepticum (MG) is the most important pathogenic Mycoplasma spp. causing avian mycoplasmosis and brought about huge economic losses to poultry industry in Bangladesh. The present study was undertaken to know the seroprevalence of MG in layer birds in three different geographical areas of southern Barishal division, Bangladesh. Total 310 sera samples were collected from wing vein of 30 farms for this study. Sera samples were tested with Rapid Serum Agglutination (RSA) for MG using commercial Antigen Kit (manufactured by Lillidale Diagnostic) to detect the presence of antibodies against MG. The overall seroprevalence of MG by RSA was 36.13%. Seroprevalence of MG infection was dominant in winter season (45.54%) and significantly highest occurrence was recorded in age groups from 20-40 weeks of layer chickens (51.79%). Serological investigation in three different upazila of Barishal division showed the highest infection rate (45.26%) in medium scale flocks (1000-3000) in comparison to (21.43%) small (<1000) flocks. The seroprevalence of MG was highest in Swarupkathi (44.38%) than in Barishal Sadar (26%) and Banaripara upazila (28%). Biosecurity and managemental failure is the overall risk factor in all types of farm due to lack of proper knowledge among farmer. This study reveals the current scenario of mycoplasmosis in layer birds of three different areas of Barishal division. Asian J. Med. Biol. Res. 2021, 7 (3), 292-297


1970 ◽  
Vol 37 (1) ◽  
pp. 17-22
Author(s):  
SS Islam ◽  
MB Hossain ◽  
MKA Khan

The study was conducted to study the effects of genotype and age of hen and hatching season on hatchability of egg. Hatching eggs were collected from hens having three genotypes viz. White Leghorn (WLH), Rhode Island Red (RIR) and Fayoumi covering three age groups (28 to 40, 41 to 60 and 61 weeks & above) at winter, monsoon and summer seasons. The study revealed that the highest (P<0.05) average hatchability was observed in winter (78.62%) followed by monsoon (76.70%) and summer (75.79%). Among different genotypes the highest (P<0.001) hatchability was found in Fayoumi (78.34%) followed by White Leghorn (76.48%) and Rhode Island Red (74.59%). Considering the age of hen, highest (P<0.05) hatchability was found in the age group between 41 to 60 weeks (77.99%) followed by 61 weeks and above (76.49%) and 28 to 40 weeks (75.71%) of age. From the findings it can be concluded that the winter season was suitable for hatching of eggs and the performance of Fayoumi hen was better in terms of hatchability of egg. On the other hand, the age of hen between 41 to 60 weeks was better for producing hatching eggs. DOI: http://dx.doi.org/10.3329/bjas.v37i1.9863 BJAS 2008; 37(1): 17-22


2020 ◽  
Vol 43 (1) ◽  
pp. 75-80
Author(s):  
Md Zulfekar Ali ◽  
Mohammad Moktader Moula ◽  
Zafar Ahmed Bhuiyan ◽  
Muhammad Tariq Javed

AbstractChicken astroviruses (CAstV) are enteric viruses of poultry causing gastroenteritis, malabsorption, gout and white chick disease commonly known as runting-stunting syndrome (RSS). It can affect the wide range of poultry birds, especially chicken, turkey and duck worldwide. To our best knowledge there is no published report on presence of antibodies against CAstV in Bangladesh. Therefore, the study aimed to detect the presence of CAstV antibodies in broilers and sonali chickens (a cross-bread) in Bangladesh through a cross-sectional survey. A total of 454 blood samples from 66 flocks of broiler (n=343) and sonali chickens (n=111) of different ages were obtained during 2017 from four districts. The birds were healthy but were not vaccinated against CAstV. The samples were tested for specific antibodies against CAstV Group B by using commercially available ELISA kit. Overall, 16.74% (76/454) samples and 34.84% (23/66) flocks were positive for CAstV antibodies. The seroprevalence of CAstV was significantly (p=0.001) higher in sonali chickens (36.96%) than broiler (10.20%), while it was significantly higher (p=0.001) in birds of Bogura district (36.94%) than the other three districts. Regarding the age groups, seroprevalence was insignificantly (p=0.192) higher in sonali chicken before laying age (45%) than during laying age (27.45%). Regarding the seasons, CAstV infection was prevalent significantly (p=0.001) higher in winter season. Thus, the present study indicated the presence of CAstV in poultry in Bangladesh, so further studies are required to find out the magnitude of the problem in the country.


2018 ◽  
Vol 8 (4) ◽  
pp. 65-69
Author(s):  
Mao Nguyen Van ◽  
Dong Tran Nam

Background: Pigmented tumour of the skin is one of the common tumour in human including the benign pigmented tumours (more common) called Nevi tumours and the malignant one called melanoma which was less frequent but the most poor in prognosis. In addition, the others not belonging to these group had the same clinical appearance, so the application of histopathology and immunohistochemistry for the definitive diagnosis was indespensible. Objectives: 1. To describe the macroscopic features of the pigmented tumoral-like lesions; 2. To classify the histopathologic types of the pigmented cell tumours and the other pigmented tumours of the skin. Materials and Method: Cross-sectional research on 55 patients diagnosed as pigmented tumoral lesions by clinician, then all definitively diagnosed by histopathology combining the immunohistochemistry in difficult cases. Results: There was no difference in gender, the disease was discovered most common in adult, especially with the age over 51 years old (58.1%). the most region located was in the face accounting for 60%, following the trunk and limbs (14.6%, 12.8% respectively). All 3 malignant melanomas happened in foot. The most common color of the lesions was black (65.4%), the other ones were rose, grey and blue. Histopathology and immunohisthochemistry showed that the true pigmented cell tumours were 52.6% encompassing benign ones (Nevi tumour) (41.8%), melanoma (5.4%) and lentigo (5.4%). 47.4% was not the true pigmented cell tumour including pigmented basocellular carcinoma (36.4%) and the others less common as histiofibromas, acanthoma and papilloma. Conclusion: the pigmented tumoral-like lesions of the skin could be the true pigmented cell tumours and the others, so the application of the histopathology and the immunohistochemistry after the clinical discovery helps to determine and classify the disease definitely and for the best orientation of treatment as well. Key words: skin tumour, benign pigmented tumour (Nevi), malignant pigmented tumour (melanoma), pigmented basocellular carcinoma


Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 78
Author(s):  
Anja Dörschug ◽  
Julian Schwanbeck ◽  
Andreas Hahn ◽  
Anke Hillebrecht ◽  
Sabine Blaschke ◽  
...  

Serological assays can contribute to the estimation of population proportions with previous immunologically relevant contact with the Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) virus. In this study, we compared five commercially available diagnostic assays for the diagnostic identification of SARS-CoV-2-specific antibodies. Depending on the assessed immunoglobulin subclass, recorded sensitivity ranged from 17.0% to 81.9% with best results for immunoglobulin G. Specificity with blood donor sera ranged from 90.2% to 100%, with sera from EBV patients it ranged from 84.3% to 100%. Agreement from fair to nearly perfect was recorded depending on the immunoglobulin class between the assays, the with best results being found for immunoglobulin G. Only for this immunoglobulin class was the association between later sample acquisition times (about three weeks after first positive PCR results) and positive serological results in COVID-19 patients confirmed. In conclusion, acceptable and comparable reliability for the assessed immunoglobulin G-specific assays could be shown, while there is still room for improvement regarding the reliability of the assays targeting the other immunoglobulin classes.


1970 ◽  
Vol 34 (3) ◽  
pp. 425-434 ◽  
Author(s):  
PK Malaker ◽  
IH Mian

The efficacy of seed treatment and foliar spray with fungicides in controlling black point incidence of wheat seeds was evaluated in the field. Two seed treating fungicides, namely Vitavax-200 and Homai-80WP were used @ 0.25% of dry seed weight and foliar spray with Tilt-250EC (0.05%) was applied in six different schedules. Untreated and unsprayed controls were also maintained. Seed treatment with either Vitavax-200 or Homai-80WP significantly increased plant population and grain yield, but none of them was found effective in reducing black point incidence. On the other hand, foliar sprays with Tilt-250EC under all the spray schedules except spraying at 70 and 90 DAS significantly minimized the disease severity over unsprayed control. Among the different spray schedules, spraying at 30, 40, 50, 60, 70, 80, and 90 DAS appeared to be most effective, which was similar to spraying at 30, 45, 60, 75, and 90 DAS in reducing black point incidence and increasing grain yield. Economic analysis on yield advantage showed that the highest additional gross margin of Tk. 6120/ha with BCR 2.57 was obtained from five sprays applied at 30, 45, 60, 75, and 90 DAS. Key Words: Seed treatment, foliar spray, black point, wheat. DOI: 10.3329/bjar.v34i3.3968 Bangladesh J. Agril. Res. 34(3) : 425-434, September 2009


1970 ◽  
Vol 6 (1) ◽  
pp. 45-51 ◽  
Author(s):  
T Farjana ◽  
KR Islam ◽  
MMH Mondal

 A study was conducted to investigate the population density of helminth parasites in domestic ducks (Anas boschas domesticus) in relation to host's age, sex, breed and seasons of the year from March 2002 to May 2003. A total of 300 ducks were collected from different villages of Netrokona and Mymensingh districts of Bangladesh and autopsied to collect the parasites and counted to determine the population density of parasites. Off 300 ducks examined, 290 (96.66%) were infected with 17 species of helminth parasites in which 11 species were trematodes, 4 were cestodes and 2 nematodes. Among the parasites, density of cestodes was the highest (33.15±5.26), followed by trematodes (5.98±1.32); and nematodes (2.95±0.68). Mean density of parasites increased with the increase of age (young: 21.23±1.09, adult: 26.18±2.14 and old: 27.87±2.98) while the mean density of most of the helminth parasites was higher in female ducks (31.35±4.72) than in males (27.52±3.32). Indigenous ducks (33.72±3.61) were infected with the highest load of helminths than Khaki Campbell breed (29.61±4.32) of ducks. Mean density of most trematodes (5.42±0.80) were highest in winter season whereas mean density of all cestodes (48.43±4.85) and nematodes (4.13±1.76) were highest in summer.  The present study suggests that age, sex, breed of ducks and seasons of the year influence the parasitic infection to a greater extend. Key words: Population density, helminths, duck, Bangladesh DOI = 10.3329/bjvm.v6i1.1338 Bangl. J. Vet. Med. (2008). 6 (1): 45-51


1982 ◽  
Vol 62 (3) ◽  
pp. 751-757 ◽  
Author(s):  
J. A. BASARAB ◽  
R. T. BERG ◽  
J. R. THOMPSON

The in vitro glucose consumption and lactic acid production by erythrocytes from 20 cattle of a Beef Synthetic (SY) breed group and 25 cattle of a double-muscled (DM) breed group were determined. There were three age groups and two sexes within each breed group. Animals within the DM breed group were categorized as either phenotypically normal- to moderate-muscled (DM carriers) or extreme-muscled (extreme DM) based on the phenotypic expression of the double-muscling trait and on their breeding history. Both DM phenotypes had higher (P < 0.01) erythrocyte glucose consumption and lactic acid production than normal-muscled, noncarrier cattle of the SY breed group. Extreme DM cattle were not different (P > 0.05) in either their erythrocyte glucose consumption or lactic acid production compared with DM carriers. No difference (P > 0.05) due to breed or phenotype was observed in the molar ratio of lactic acid produced to glucose consumed by erythrocytes. These results suggest that carriers of the Double Muscled Syndrome, regardless of phenotypic expression of the double-muscling trait, have a higher rate of erythrocyte glycolysis than normal cattle. Key words: Cattle, double muscled, erythrocyte, glucose, lactic acid


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