scholarly journals HISTOMORPHOLOGICAL STUDY OF THE MAJOR LYMPHOID TISSUES IN INDIGENOUS DUCKLINGS OF BANGLADESH

2012 ◽  
Vol 9 (1) ◽  
pp. 53-58
Author(s):  
N Sultana ◽  
MZI Khan ◽  
MA Wares ◽  
MA Masum
Keyword(s):  
Connective Tissue ◽  
Plasma Cells ◽  
Immunocompetent Cells ◽  
Lymphoid Tissues ◽  
White Pulp ◽  
Drug Induced ◽  
Connective Tissue Capsule ◽  
Reticular Fibers ◽  
Reticular Cells ◽  
Mucosal Folds

A histomorphological study was performed in the major lymphoid tissues (thymus, bursa of Fabricus and spleen) of the six 21-day-old indigenous ducklings of Bangladesh by H & E staining method during the period from March to May 2011. In the present study, it was observed that the thymus was enclosed by a thin connective tissue capsule. Numerous fine septa of connective tissue originated from the capsule and divided the organ into incompletely separated lobules. Each lobule organized into a peripheral cortex and a central medulla. The bursa of Fabricus was consisted of mucosal folds (plicae). Numerous follicles filled the lamina propria of each fold and each bursal follicle was composed a peripheral cortex and a central medulla. A layer of undifferentiated epithelial cells occupied the periphery of the medulla, which was separated from the cortex by a capillary layer. The darkly stained cortex was composed of many closely packed small lymphocytes. The paler medulla contained fewer cells of various sizes. The spleen was surrounded by a thick splenic capsule and there were a small number of trabeculae. The white pulp was composed of network of reticular cells and reticular fibers within various size lymphocytes and plasma cells were diffusely distributed. The red pulp of the spleen was formed from venous sinuses and anastomosing cord of reticular cells, macrophages, lymphocytes and blood cells. The length and breadth of the thymic lobules, bursal follicles and white pulp of the spleen were 226.68 and 165.78cm, 204.45 and 138.23cm, and 129.05 and 103.43cm respectively. The result of the present work revealed that the immunocompetent cells were arranged scatteredly or densely as an unorganized lymphatic nodules in the lymphoid tissues. The length and breadth of the thymic lobules were higher followed by bursal follicle and splenic white pulps were varied within the lymphoid tissues and even one another in indigenous ducklings. The results of the present study indicate that the architecture and distribution of lymphocytes and lymphoid follicles of ducklings is very close to the chicken and this study might be helpful to understand the changes in the frequency of the population of immunocompetent cells in drug induced, vitamin and mineral supplemented or hormone treated duck in future.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11212 Bangl. J. Vet. Med. (2011). 9(1): 53-58 

scholarly journals Histomorphological study of the lymphoid tissues of broiler chickens

1970 ◽  
Vol 4 (2) ◽  
pp. 87-92 ◽  
Author(s):  
S Akter ◽  
MZI Khan ◽  
MR Jahan ◽  
MR Karim ◽  
MR Islam
Keyword(s):  
Connective Tissue ◽  
Broiler Chickens ◽  
Plasma Cells ◽  
Splenic Tissue ◽  
Bursa Of Fabricius ◽  
Immunocompetent Cells ◽  
Lymphoid Tissues ◽  
White Pulp ◽  
Cecal Tonsil ◽  
Reticular Cells

Topology and histology were performed in the lymphoid tissues (thymus, bursa of Fabricius, spleen and cecal tonsils) of the fifteen 28-days-old "Kasilla" broilers by observation of H & E stained sections in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh during the period from October to December 2005. In the present study, it was observed that the thymus was enclosed by a thin connective tissue capsule. Numerous fine septa of connective tissue originated from the capsule and divide the organ into incompletely separated lobules. Each lobule organized into a peripheral cortex and a central medulla. The population of the immunocompetent cells (lymphocytes and immunoglobulin containing plasma cells) in the cortex were denser rather than that of medulla of the thymic lobule. The bursa of Fabricius was consisting of long thick mucosal folds (plicae). Numerous follicles filled the lamina propria of each fold and each bursal follicle was composed a peripheral cortex and a central medulla. The population of the immunocompetent cells in the cortex of the bursal follicle were denser rather than that of medulla of the bursal follicle. The spleen was surrounded by a thick splenic capsule and there was a small number of trabeculi. The network of the splenic tissue was consisting of a network of reticular cells and fibers and was arranged into red pulps, which were scatteredly distributed within the white pulps. The white pulp was composed of network of reticular cells and reticular fibers within which the immunocompetent cells were diffusely distributed. It contained sheathed arteries and lymphatic nodules. The red pulp of the spleen was formed from venous sinuses and anastomosing cord of reticular cells, macrophages, lymphocytes and blood cells. Cecal tonsil was composed of four histological layers i.e. tunica mucosa, submucosa, muscularis and serosa. Their lining epithelium was simple columnar epithelium. More diffuse lymphoid tissue and unorganized lymphatic nodules were present both in the mucosa and submucosa of the cecal tonsil of broiler. The length and breadth of the thymic lobules were 629.30 ± 118.95 µm and 376.03 ± 98.92 µm, bursal follicles 468.83 ± 52.26 µm and 240.70 ± 34.19 µm, white pulp of the spleen 112.62 ± 13.25 µm and 89.42 ± 12.20 µm, lymphatic nodules of the cecal tonsil 255.20 ± 20.46 µm and 186.08 ± 24.90 µm respectively. The result of the present study revealed that the immunocompetent cells were arranged scatteredly or densely and as unorganized or organized lymphatic nodules in the lymphoid tissues and the length and breadth of the thymic lobule, bursal follicle, splenic white pulp and lymphatic nodule of cecal tonsils were varied within the lymphoid tissues and even one another. Key words: Histology, lymphoid tissues, immunocompetent cells, broilersDOI = 10.3329/bjvm.v4i2.1289Bangl. J. Vet. Med. (2006). 4 (2): 87–92

scholarly journals Anatomical and histological changes in the spleen of post hatching indigenous chicken in Iraq

2018 ◽  
Vol 41 (2) ◽  
pp. 174-178
Author(s):  
Rabab Adnan Hamza
Keyword(s):  
Plasma Cells ◽  
Smooth Muscles ◽  
White Pulp ◽  
Microscopical Examination ◽  
Indigenous Chicken ◽  
Connective Tissue Capsule ◽  
Lymphatic Organ ◽  
Lymphatic Follicle ◽  
Staining Techniques ◽  
Red Pulp

     The structure of the indigenous chickens spleen during the post-hatching period was determined by gross and light microscopical examination by using Hematoxylin and eosin and Massons Trichrome staining techniques. At one day old chicks the spleen was rounded in shape, pink in color. At two weeks old chicks the spleen was triangular in shape. At the progress of the aged the color of spleen became red-brown. In all ages the spleen consisted of white pulp and red pulp which were fused together. The spleen was encapsulated by thin connective tissue capsule contain few smooth muscles, the trabiculi were rare and thin. The red pulp consisted of venous sinuses surrounded by lymphatic cords. The white pulp consisted of peri-artery lymphoid sheath, peri-venous lymphoid sheath, peri ellipsoid lymphoid sheath, and Lymphatic follicles. The appearance of these elements was age dependant. At the first week of age the peri-artery lymphoid sheath and peri-venous lymphoid sheath were developed. At the third week, the peri ellipsoid lymphoid sheath, Lymphatic follicles were noticed and the plasma cells were scattered in the white pulp in addition to the lymphocytes. At one month of age, the germinal center appeared in some lymphatic follicle. The present study revealed that the spleen was well developed lymphatic organ at the age of three weeks.

scholarly journals THE FUNCTIONS OF THE THYMUS SYSTEM AND THE BURSA SYSTEM IN THE CHICKEN

1966 ◽  
Vol 123 (1) ◽  
pp. 75-102 ◽  
Author(s):  
Max D. Cooper ◽  
Raymond D. A. Peterson ◽  
Mary Ann South ◽  
Robert A. Good
Keyword(s):  
Plasma Cells ◽  
Germinal Centers ◽  
Cell System ◽  
Delayed Hypersensitivity ◽  
Bursa Of Fabricius ◽  
Surgical Removal ◽  
Lymphoid Tissues ◽  
White Pulp ◽  
Dependent Development ◽  
Graft Versus Host

The bursa of Fabricius and the thymus are "central lymphoid organs" in the chicken, essential to the ontogenetic development of adaptive immunity in that species. Surgical removal of one or both of these organs in the newly hatched chicken, followed by sublethal X-irradiation the next day, has permitted recognition of two morphologically distinct cell systems in the "peripheral lymphoid tissues" of the spleen, gut, and other organs, and clear definition of the separate functions of each cell system. The thymus-dependent development is represented morphologically by the small lymphocytes of the circulation and the white pulp type of development in the tissues. As in mammals, the thymus-dependent tissues of the chicken are basic to the ontogenesis of cellular immunity: graft versus host reactions, responses of delayed hypersensitivity and homograft rejection; and play a less clearly defined role in the antibody response to at least some antigens. Thymectomized-irradiated chickens are deficient in all these responses, and grow more slowly than any of the other experimental groups. In these animals germinal centers, plasma cells, and capacity for immunoglobulin synthesis remain intact. The bursa-dependent development is represented morphologically by the larger lymphocytes of the germinal centers and the plasma cells, and functionally by the immunoglobulins. Bursectomized-irradiated chickens are agammaglobulinemic and unable to produce detectable antibody despite intense, repeated stimulation with bovine serum albumin and Brucella abortus organisms. The thymus-dependent development in these animals seems to be normal; they have adequate numbers of lymphocytes in the circulation and tissues, are able to reject skin homografts, though more slowly than usual, and to exercise graft versus host reactions. The short life span of these chickens has precluded adequate study of responses of delayed hypersensitivity. There was no evidence of significant impairment of reticuloendothelial function in either the bursectomized-irradiated or the thymectomized-irradiated group, as judged by the clearance of colloidal gold and I131-tagged keyhole limpet hemocyanin.

Gross Morphological and Light Microscopic Studies of the Spleen of Malayan Sun Bear (Helarctos malayanus)

2021 ◽  
Author(s):  
P.C. Kalita ◽  
A. Kalita ◽  
O.P. Choudhary ◽  
P.J. Doley ◽  
S. Debroy ◽  
...  
Keyword(s):  
Basic Research ◽  
White Pulp ◽  
Lateral Abdominal Wall ◽  
Helarctos Malayanus ◽  
Connective Tissue Capsule ◽  
Splenic Parenchyma ◽  
Reticular Fibers ◽  
Microscopic Studies ◽  
Sun Bear ◽  
Conservation Plans

Background: Bear specialist group recommended that the basic research on the Malayan sun bear is the highest priority need. Without such information, the establishment and implementation of scientifically-sound conservation plans is difficult. Therefore, present study was designed to provide information on gross morphological and light microscopic architecture of the spleen.Methods: The present study was conducted on the spleen of one Malayan sun bear. After doing the gross parameters the tissues were fixed in 10% neutral buffered formalin and were processed for light microscopic studies. Blocks were cut at 6μ thickness by Leica Semimotorized Rotary Microtome and stained by Harris’ haematoxylin and eosin for routine study. Result: The spleen of Malayan sun bear was located in the left hypogastric region and entirely intrathoracic as the stomach was almost empty. The parietal surface faces the diaphragm and left lateral abdominal wall, whereas the visceral surface was divided into gastric face and intestinal face by the ridge like hilus. The spleen of Malayan Sun Bear was surrounded by a thick connective tissue capsule invested by the peritoneum. The capsule, trabeculae and reticular fibers support the splenic parenchyma composed of a red pulp and a white pulp.

scholarly journals Immunohistological localization of alpha 1-microglobulin in normal rat tissues.

1984 ◽  
Vol 32 (7) ◽  
pp. 717-723 ◽  
Author(s):  
P Bouic ◽  
C Vincent ◽  
J P Revillard
Keyword(s):  
Dendritic Cells ◽  
Plasma Cells ◽  
Rabbit Antiserum ◽  
Secretory Component ◽  
Differential Staining ◽  
Secretory Cells ◽  
Lymphoid Tissues ◽  
White Pulp ◽  
Rat Tissues ◽  
Cytoplasmic Staining

The tissue distribution of rat alpha 1-microglobulin (alpha 1-m) was studied by indirect immunofluorescence in various rat tissues using a polyvalent rabbit antiserum to the purified antigen and a monoclonal antibody (H23) to the human homologue, in parallel with a polyclonal anti-rat IgA antiserum. It was found that all tissues stained by anti-IgA were also alpha 1-m positive; these tissues included tissues of the stomach, duodenum, ileum, colon, pancreas, trachea, esophagus and jejunum. However, the observation that IgA plasma cells as well as secretory cells, while positively stained by anti-IgA, are alpha 1-m negative suggests that the association between IgA and alpha 1-m occurs at a postsecretory stage, after the IgA molecules have been transported across the epithelial cells. Additionally, hepatocytes were intensely stained by anti-alpha 1-m antibodies, indicating that the liver, as already suggested by metabolic studies on isolated guinea-pig liver explants, may be responsible for the synthesis of this protein. Among lymphoid tissues, an intense and homogeneous staining was observed in the thymus and the white pulp of the spleen. Sections of lymph nodes, however, showed differential staining; apart from a few isolated dendritic cells in the mantle region of the lymphoid follicles, the germinal centers and medullary cords showed no staining with anti-alpha 1-m antibodies. The paracortical cells, macrophages in the subcapsular sinus, and interfollicular lymphocytes showed intense cytoplasmic staining with anti-alpha 1-m antibodies. In other tissues, macrophages, monocytes, tissue histiocytes, and dendritic cells were alpha 1-m positive. Although they confirm the presence of alpha 1-m in the lymphoid tissues, as already reported in man, these results show that the protein is also present in hepatocytes and in exocrine fluids containing IgA. Since alpha 1-m, like secretory component, can bind to IgA to form stable complexes, these two heavily glycosylated proteins may have similar biologic properties.

scholarly journals Connective Tissue Metabolism and Gingival Overgrowth

2004 ◽  
Vol 15 (3) ◽  
pp. 165-175 ◽  
Author(s):  
P. C. Trackman ◽  
A. Kantarci
Keyword(s):  
Extracellular Matrix ◽  
Connective Tissue ◽  
Oral Hygiene ◽  
Tissue Specificity ◽  
Gingival Fibroblast ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Connective Tissue Metabolism ◽  
Matrix Metabolism ◽  
Systemic Health

Gingival overgrowth occurs mainly as a result of certain anti-seizure, immunosuppressive, or antihypertensive drug therapies. Excess gingival tissues impede oral function and are disfiguring. Effective oral hygiene is compromised in the presence of gingival overgrowth, and it is now recognized that this may have negative implications for the systemic health of affected patients. Recent studies indicate that cytokine balances are abnormal in drug-induced forms of gingival overgrowth. Data supporting molecular and cellular characteristics that distinguish different forms of gingival overgrowth are summarized, and aspects of gingival fibroblast extracellular matrix metabolism that are unique to gingival tissues and cells are reviewed. Abnormal cytokine balances derived principally from lymphocytes and macrophages, and unique aspects of gingival extracellular matrix metabolism, are elements of a working model presented to facilitate our gaining a better understanding of mechanisms and of the tissue specificity of gingival overgrowth.

scholarly journals THE EFFECT OF EPITHELIAL REMNANT AND WHOLE ORGAN GRAFTS OF THYMUS ON THE RECOVERY OF THYMECTOMIZED IRRADIATED MICE

1969 ◽  
Vol 129 (6) ◽  
pp. 1235-1246 ◽  
Author(s):  
Esther F. Hays
Keyword(s):  
Direct Action ◽  
Lymphoid Cells ◽  
Lymphoid Tissues ◽  
Functional Development ◽  
Reticular Cells ◽  
Microscopic Morphology ◽  
Two Parameters ◽  
A Minor ◽  
Cellular Components

Work has been presented which suggests that thymus epithelial reticular cells are not effective in restoring the microscopic morphology of lymphoid tissues and their immunologic capacities. They function in recruiting precursors of thymus lymphocytes from the host animals to produce an organ which, after it becomes architecturally normal, can reconstitute the defective host. Intact thymus grafts in situ from 10–14 days, but not for shorter periods of time, have been shown to result in a return toward normal of these two parameters. Evidence is offered to show that few dividing cellular components in the lymphoid tissue originate from the thymus remnant grafts, and that a minor cellular component is contributed by the intact grafts. These data support the concept that the structural and functional development of the lymphatic tissue in thymectomized animals is dependent on thymus lymphoid cells and/or their products, and that the epithelial-reticular cells do not have a direct action in peripheral lymphoid reconstitution.

scholarly journals CELLULAR SITES OF FORMATION OF GAMMA GLOBULIN

1957 ◽  
Vol 106 (5) ◽  
pp. 627-640 ◽  
Author(s):  
L. G. Ortega ◽  
R. C. Mellors
Keyword(s):  
Germinal Center ◽  
Gamma Globulin ◽  
Plasma Cells ◽  
Serum Proteins ◽  
Fluorescent Antibody ◽  
Germinal Centers ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Reticular Cells ◽  
Parenchymal Cells

The cellular sites of formation of γglobulin in lymphatic tissues of man and in a representative human lymphoid infiltrate have been studied by fluorescent antibody technique. The findings indicate that γ-globulin is formed in the germinal centers of lymphatic nodules and in the cytoplasm of mature and immature plasma cells of two types—those with and those without Russell bodies. The germinal center cells that synthesize γ-globulin have been designated "intrinsic" cells to distinguish them from the medium and large lymphocytes, and the primitive reticular cells that occur elsewhere and do not produce γ-globulin. Unlike the plasma cells, which function as individual units, the intrinsic cells apparently form γ-globulin only when they are arranged in discrete aggregations. The function, the blood supply, and the systematic cellular arrangement of germinal centers justifies the postulate that they are miniature organs of internal secretion of γ-globulin. The release of γ-globulin from its sites of formation appears to be accomplished by holocrine and apocrine secretion. Presumably, these secretory mechanisms are adaptations required for the production of antibody since they have not been described in parenchymal cells that form the other serum proteins. The cells found to form γ-globulin appear to be identical with those previously shown to form specific antibody in response to a variety of antigens in the experimental animal. This evidence indicates that normal γ-globulin, if it exists, originates in the same cells that produce antibody. It is suggested, also, that each of the 3 morphologically distinct categories of cells that synthesize γ-globulin represents a response to a particular form of antigenic stimulation. Nuclear participation in the process of γ-globulin synthesis was not detected by the technique employed.

scholarly journals High-Dimensional Immunophenotyping of Murine T-cell and B-cell Subsets

2020 ◽  
Author(s):  
Kyle T. Mincham ◽  
Jacob D. Young ◽  
Deborah H. Strickland
Keyword(s):  
T Cell ◽  
B Cell ◽  
Plasma Cells ◽  
Effector Memory ◽  
High Dimensional ◽  
Cell Populations ◽  
Lymphoid Tissues ◽  
Murine Models ◽  
Follicular Helper ◽  
B Cell Subsets

Purpose and appropriate sample typesThis 19-parameter, 18-colour flow cytometry panel was designed and optimised to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell and B-cell subsets within murine lymphoid tissues (Table 1). Cellular populations identified by employing this OMIP include 4 major subsets of B-cells (memory, activated, plasma cells and plasmablasts) and 7 major subsets of CD4+ T-cells (naïve, central memory, effector memory, helper, regulatory, follicular helper and follicular regulatory). Staining was performed on freshly isolated splenocytes from 21-day-old neonatal BALB/c mice, however due to the omission of mouse strain-specific markers, this OMIP can be implemented across a range of murine models where in-depth immunophenotyping of the diverse repertoire of T-cell and B-cell populations localised within lymphoid tissues is required.

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