scholarly journals Potential fungicides and plant extracts against Fusarial wilt of brinjal

2015 ◽  
Vol 24 (2) ◽  
pp. 209-213
Author(s):  
MA Bashar ◽  
Romana Akter ◽  
KS Hossain
Keyword(s):  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Pathogenic Fungi ◽  
Zingiber Officinale ◽  
Significant Inhibition ◽  
Asparagus Racemosus ◽  
Aqueous Extracts ◽  
In Vitro Growth ◽  
Plant Parts

Antifungal potentials of nine fungicides and eight plant parts extracts were tested in opposition to Fusarium oxysporum Schlecht. and F. solani (Mart.) Sacc. – the two pathogens, isolated from wilted roots of brinjal plants. Efficiency gradients observed in the present study expressed that Bavistin and Tall were the best inhibiting agent against the in vitro growth of the test pathogenic fungi. Among aqueous extracts of eight plant parts significant inhibition of the growth of the pathogens was observed with Azadiracta indica (leaf), Zingiber officinale (rhizome) and Asparagus racemosus (root) at 20% concentration. Dhaka Univ. J. Biol. Sci. 24(2): 209-213, 2015 (July)

scholarly journals In vitro control of Fusarium solani and F. oxysporum the causative agent of brinjal wilt

2014 ◽  
Vol 23 (1) ◽  
pp. 53-60 ◽  
Author(s):  
MA Bashar ◽  
Mina Chakma
Keyword(s):  
Soil Fungi ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Ocimum Sanctum ◽  
Asparagus Racemosus ◽  
Efficient Inhibitor ◽  
Plant Parts ◽  
Datura Metel ◽  
Antagonistic Potential

Seven soil fungi viz. Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Penicillium sp., Trichoderma harzianum and T. viride associated with the rhizosphere, non?rhizosphere and rhizoplane of brinjal plants were selected to observe their antagonistic potential against the test fungi Fusarium oxysporum and F. solani . Out of seven soil fungi T. harzianum was found most effective to control the growth of both the test fungi. Plant parts extract of Allium sativum, Asparagus racemosus, Azadirachta indica, Cassia alata, Ocimum sanctum, Zingiber officinale and Datura metel were evaluated for their in vitro efficacy at 5, 10 and 20% concentration against the test fungi. Datura metel, and C. alata and A. indica was found most efficient inhibitor of F. solani and F. oxysporum, respectively. Five fungicides viz., agridazim 50 wp, cozeb 80 wp, newban 50 wp, sunvit 50 wp and vitavax 200 B were evaluated for their in vitro efficacy at 500 ppm concentration against F. solani and F. oxysporum. Vitavax 200B was found most efficient inhibitor of both the test fungi. DOI: http://dx.doi.org/10.3329/dujbs.v23i1.19826 Dhaka Univ. J. Biol. Sci. 23(1): 53-60, 2014

scholarly journals In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties

2018 ◽  
Vol 44 (2) ◽  
pp. 251-259
Author(s):  
Pranami Chowdhury ◽  
MA Bashar ◽  
Shamim Shamsi
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Fusarium Moniliforme ◽  
Mangifera Indica ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Asparagus Racemosus ◽  
Rice Varieties ◽  
Datura Metel

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.

scholarly journals Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CLPro) Activity

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1503
Author(s):  
Carla Guijarro-Real ◽  
Mariola Plazas ◽  
Adrián Rodríguez-Burruezo ◽  
Jaime Prohens ◽  
Ana Fita
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Hydrolysis Product ◽  
Resonance Energy ◽  
Significant Inhibition ◽  
Main Role ◽  
Turmeric Extract ◽  
Ic50 Value

Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CLPro) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CLPro activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CLPro activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 µg mL−1 displayed significant inhibition of the 3CLPro activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC50 value of 15.74 µg mL−1 was calculated for turmeric extract. Commercial curcumin inhibited the 3CLPro activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CLPro activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CLPro inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC50 value of 41.43 µg mL−1. The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials.

scholarly journals Phytochemical screening and evaluation of antioxidant capacities of Allium cepa, Allium sativum, and Monodora myristica using in vitro and in vivo models

2021 ◽  
Vol 1 (1) ◽  
pp. 41-52
Author(s):  
Chioma E. Irozuru Irozuru ◽  
Janet O. Olugbodi ◽  
Uche Okuu Arunsi ◽  
Olusola Ladeji
Keyword(s):  
Ascorbic Acid ◽  
Allium Cepa ◽  
Plant Extracts ◽  
Allium Sativum ◽  
Reducing Power ◽  
Aqueous Extracts ◽  
In Vivo Models ◽  
Monodora Myristica

Background: Allium cepa, Allium sativum, and Monodora myristica are commonly sourced food condiments in every household in Nigeria. In the present study, we investigated the phytochemical compositions, in vitro and in vivo antioxidant activity of these plants. Methods: The aqueous extracts from the A. cepa, A. sativum, and M. myristica were evaluated for phytochemical composition using standard protocols while the antioxidant activities were evaluated using the reducing power assay. Forty-five (45) Male Wistar rats (weighing 185±10 g) were divided into five groups (n=9) and were orally administered with 100 mg/kg BW each of A. sativum, M. myristica, A. cepa, and ascorbic acid while the control group received 0.5 mL/kg BW distilled water alone. Animals (n=3) from each group were sacrificed after the 20th, 25th, and 30th days of oral administration. The blood and tissue samples were collected for the analysis of biochemical parameters. Result: Our results revealed the presence of flavonoids, alkaloids, tannins, saponins, and terpenes in the plant extracts. A. sativum had the highest reducing power capacity followed by M. myristica and then A. cepa. The in vitro antioxidants activities demonstrated by the plant extracts were higher than that of ascorbic acid but less than butylated hydroxytoluene. In vivo antioxidant studies showed a marked increase (p<0.05) in the level of catalase with a concurrent decrease (p<0.05) in the levels of MDA and H2O2 in the liver and kidney of rats administered with aqueous extracts of the condiments compared to the normal control and ascorbic acid in the following order control < ascorbic acid < A. cepa < M. myristica < A. sativum. Conclusion: Based on these findings, we infer that the aqueous extracts of A. cepa, A. sativum, and M. myristica are rich in antioxidants and as a result could serve as promising novel functional foods and nutraceuticals

scholarly journals In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina.

2017 ◽  
Vol 6 (9) ◽  
pp. 1676 ◽  
Author(s):  
Ramaraju Cherkupally ◽  
Srinivasa Reddy Kota ◽  
Hindumathi Amballa ◽  
Bhumi Narasimha Reddy
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Aloe Vera ◽  
Datura Stramonium ◽  
Macrophomina Phaseolina ◽  
Calotropis Procera ◽  
Fungitoxic Effect

The antifungal activity of aqueous extracts of nine plants viz, Azadirachta indica, Parthenium hysterophorus, Momordica charantia, Allium sativum, Eucalyptus globules, Calotropis procera, Aloe vera, Beta vulgaris and Datura stramonium were assessed in vitro against Fusarium oxysporum f. sp. melongenae, Rhizoctonia solani and Macrophomina phaseolina, the soil borne phytopathogens. The assessment of fungitoxic effect was carried out by using three different concentrations i.e., 5, 10 and 20% against the test fungi, in terms of percentage of mycelial growth inhibition. The extract of A. sativum completely inhibited the mycelial growth of M. phaseolina at all the concentrations. The extracts of D. stramonium and E. globulus inhibited the mycelial growth of R. solani of 72%, and 70.7% respectively at 20% concentration, that of A. sativum, E. globulus and D. stramonium exhibited inhibition percentage of 63.3%, 61.8% and 61.1% respectively at 20% concentration on Fusarium oxysporum f. sp. melongenae. The application of plant extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.

scholarly journals In Vitro Antibacterial and Antifungal Activity and Datura Innoxia Extracts

2014 ◽  
Vol 3 (3) ◽  
pp. 173-185
Author(s):  
Adam IY Shama ◽  
YM Abd-Kreem ◽  
AA Fadowa ◽  
RM Samar ◽  
MK Sabahelkhier
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Antibacterial Drug ◽  
Aqueous Extracts ◽  
Datura Innoxia ◽  
Methanolic Extracts ◽  
Leaves And Roots

The aim of this study was evaluated the Antimicrobial Activity of extraction of Datura innoxia (Seeds, leaves and roots). Datura innoxia Seeds, leaves and roots were collected to examine their antimicrobial activity. Extracts of different parts of the plant were tested against standard microorganisms by using the agar- well diffusion method. Extracts of methanol, and aqueous of seeds, leaves and roots were prepared and tested against four types of bacteria namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris and two types of fungi namely: Aspergillus niger and Candida albicans. The methanolic and aqueous extracts of leaves showed high activities against fungi (A. niger) and less effect on the all bacteria. The methanolic extracts of seeds showed high activities against all organisms except fungi (C. albicanas), while the aqueous extracts of seeds showed no activity on the bacteria. All organisms were examined against known standard antibiotics and then compare the results of plant extracts with standard antibiotics. The results indicated that the antibacterial drug is less active than the plant extracts, while the antifungal drugs are more active than the plant extracts. DOI: http://dx.doi.org/10.3126/ije.v3i3.11077 International Journal of Environment Vol.3(3) 2014: 173-185

scholarly journals In vitro allelopathic effect of aqueous extracts of sugarcane on germination parameters of wheat

2017 ◽  
Vol 109 (2) ◽  
pp. 349 ◽  
Author(s):  
Abdul Majeed ◽  
Zahir MUHAMMAD ◽  
Manzoor HUSSAIN ◽  
Habib AHMAD
Keyword(s):  
Germination Percentage ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Wheat Growth ◽  
Plant Parts ◽  
Seedling Biomass ◽  
Mean Germination Time ◽  
Germination Indices ◽  
Germination Parameters

Allelopathy – interactions among plants for resources along with competition – is a composite phenomenon which has spacious potentials of application in agriculture. Understanding of interactions among plants, particularly cultivated crops, may be helpful in modifying crop cultivation pattern with consequent yields increments. In this study, we investigated the allelopathic effects of aqueous extracts of root, stem peels and leaves of sugarcane (<em>Saccharum officinale</em> L.) cultivar <em>51</em> at concentrations 0, 2.5, 5.0, 7.5 and 10.0 g/l on germination indices and seedling biomass of wheat (<em>Triticum aestivum </em>L.) cultivar <em>Pirsabak-2005</em>. Results demonstrated that higher concentration (10.0 g/l) of extracts of root, stem peels and leaves significantly decreased mean germination time (MGT) but increased shoot and seminal root growth and seedling dry biomass; however, germination percentage was affected neither by extract concentration nor by plant parts used in the study. Extract concentrations up to 7.5 g/l had no effect on the studied parameters of wheat. Our result suggests that sugarcane’s allelopathy demonstrates healthy effects on wheat growth and that wheat could be cultivated in sequential rotation in field conditions.

scholarly journals Two Polyketides Produced by Endophytic Penicillium citrinum DBR-9 From Medicinal Plant Stephania kwangsiensis and Their Antifungal Activity Against Plant Pathogenic Fungi

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984679 ◽  
Author(s):  
Haiyu Luo ◽  
Zhen Qing ◽  
Yecheng Deng ◽  
Zhiyong Deng ◽  
Xia’an Tang ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Membrane Integrity ◽  
Pathogenic Fungi ◽  
Significant Inhibition ◽  
Penicillium Citrinum ◽  
Plant Pathogenic Fungi ◽  
Fungal Cell ◽  
Cell Membrane Integrity ◽  
Chinese Medicinal Plant

Endophytic fungi, especially those found in medicinal plants, are widely studied as producers of secondary metabolites of biotechnological interest. In this study, on the basis of an activity-directed isolation method and spectroscopic analysis, two active polyketides, citrinin (1) and emodin (2), were isolated and identified from the fermentation of the endophytic fungus Penicillium citrinum DBR-9. This fungus was isolated from the root tubers of the traditional Chinese medicinal plant Stephania kwangsiensis. In vitro antifungal assay showed that the two polyketides displayed significant inhibition on hypha growth of tested plant pathogenic fungi with IC50 values ranging from 3.1 to 123.1 μg/mL and 3.0 to 141.0 μg/mL, respectively. In addition, the mechanism of the effects of emodin (2) on the pathogen revealed it could affect the colony morphology, destroy cell membrane integrity, and influence the protein synthesis of the tested fungal cell. This work is the first report of two polyketides-producing endophytic P. citrinum DBR-9 from the medicinal plant S. kwangsiensis. Our results present new opportunities to deeply understand the potential of these two polyketides as natural antifungal agents to control phytopathogens in agriculture.

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