scholarly journals Bio-remedial Effect of Specific Micro-organisms (Bacteria) on Used Engine Oil: A Case Study of Some Mechanic Workshops in Maiduguri Metropolitan Council (MMC)

2020 ◽  
Vol 1 (3) ◽  
Author(s):  
Ibrahim Yerima ◽  
G. A. Felix ◽  
M. I. Ahmad
Keyword(s):  
Zea Mays ◽  
Agar Medium ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Bacillus Sp ◽  
Used Engine Oil ◽  
Micro Organisms ◽  
Effective Growth

The potential of three micro-organisms (Pseudomonas, Streptococcus and Bacillus sp) were isolated from hydrocarbon contaminated soil and were evaluated for their biodegradation ability. The rate of biodegradation of the engine oil in the soil samples were exposed to used engine oil with different exposure rates of 5,10,15 and 20 years  were studied for a period of three (3) weeks under greenhouse experiment. The soil samples were obtained from four different mechanic workshops in M.M.C and they were plated on nutrients agar and oil agar medium to isolate the bacterial species from the spilled soil samples. All the micro-organisms used in this study showed their abilities to remediate soil exposed to used engine oil and the remediated soil samples were able to support the growth of Maize ( Zea mays) after 10 years  effective growth

Isolation, Detection and Estimation of Various Amylase Producing Bacteria in Various Soil Samples

2021 ◽  
Vol 08 ◽  
Author(s):  
Mohd Amin Mir ◽  
Mohammad Waqar Ashraf ◽  
Altaf Hussain ◽  
Bilal Ahmad Mir
Keyword(s):  
Plant Extracts ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Cholesterol Levels ◽  
Microbiological Methods ◽  
Ethanolic Extracts ◽  
Microbial Strains ◽  
Litmus Test ◽  
Micro Organisms

Background: Soil is an ultimate source of all types of nutrients, which have both biological and non-biological importance. Studies are being carried out to isolate various types of micro-organisms from soil which have much more importance. So in the present study, amylase producing bacteria have been isolated from various soil samples. Aim: The isolation, identification, and estimation of various microbial strains for α-amylase enzyme production and then the inhibition of the growth of these microbial stains. Methods: The bacterial strains were isolated and then identified by various microbiological methods, including Gram’s staining method followed by several biochemical methods such as, litmus test, Gelatin test and Urea agar media and by viable cells. Results: Altogether, three microbial strains were identified from the soil samples in the concerned study. The concerned strains include- Shigella, Proteus and Bacillus, respectively. The concerned microbial strains were then analyzed for the amount of amylase enzyme and it had been found that Bacillus sp produces much more amount of amylase followed by Shigella sp, and lesser amylase enzyme producing activity was found in Proteus sp. The isolated bacteria were then analysed for inhibition of their growth by water and ethanolic extracts of Cuminum cyminuni. Among the extracts, it had been found that water extracts exhibited more inhibiting capacity than the ethanolic extracts. The study also revealed that among the bacterial strains, the Shigella sp got much more affected by the concerned plant extracts followed by Proteus sp and least inhibition was observed against the Bacillus sp. Conclusion: As per the above study, it is being concluded that - three amylase producing bacteria viz Shigella, proteus, bacillus sp were isolated from the soil samples. These isolated microbial strains could be used for the decomposition of cholesterol levels in human in addition to other microbial activity. These isolated bacterial could sometimes be averse therefore their growth could be stopped by various biological and chemical substances like Gentamicin and by various Plant extracts viz, Cuminum cyminuni Plant.

scholarly journals Calcareous and Gypsum Soils Lead Removal Using Two-Steps Washing

2021 ◽  
Vol 910 (1) ◽  
pp. 012136
Author(s):  
Hazim Aziz Al-Robai ◽  
Ameer Hazim Azeez Al-Rubay ◽  
Ghaith Salah Al-Mamoori ◽  
Ali Akram Abdulateef
Keyword(s):  
Heavy Metals ◽  
Contact Time ◽  
Soil Washing ◽  
Soil Samples ◽  
Engine Oil ◽  
Lead Removal ◽  
Used Engine Oil ◽  
Solid Ratio

Abstract The soil washing technique is considered one of the quick and effective techniques for treating soil contaminated with heavy metals. In this study, EDTA-Na2 and Oxalic were used as washing solutions to removing Pb from calcareous and gypsum soils contaminated with Pb only and with Pb in addition to the used engine oil. The soil samples were contaminated with different concentrations of Pb only using Pb(NO3)2 to soils sample for each concentration separately then the sample was divided into two equal parts. The first part represents the calcareous and gypsum soil samples contaminated with Pb. The second part was taken after air-dried, and then the used engine oil was added and homogenized well. Five parameters (pH, contact time, Pb concentrations, temperature, and liquid/solid ratio) were applied to test their effect on Pb removal. In general, the results of this study varied based on the effect of the variables.

scholarly journals Biological Treatment of Used Engine Oil by Single and Mixed Bacterial Cultures Isolated from Soil of Mechanic Workshops

2018 ◽  
Vol 12 (1) ◽  
pp. 115-123
Author(s):  
Amel Hussein ◽  
Saad Khudhair
Keyword(s):  
Soil Samples ◽  
Growth Patterns ◽  
Engine Oil ◽  
Gravimetric Analysis ◽  
Bacterial Isolates ◽  
Engine Oils ◽  
Used Engine Oil ◽  
Degrading Bacteria ◽  
Spent Engine Oil ◽  
Consortium Culture

The accumulation of hydrocarbon waste, such as used engine oils in environments, has many impacts on humans and other organisms, therefore many researches were achieved to degrade or remove or consume these pollutants. The aim of the current study is to get a local bacterial isolates has high ability to degrade the spent engine oil as a single or mixed culture. Five soil samples contaminated with spent engine oil were collected from mechanic workshops in Baghdad city to isolate degrading bacteria using Bushnell Hans medium (BHM), pH 7 with 5% of used engine oil. While the growth patterns and gravimetric analysis was used to reveal the ability of these isolates to degrade spent engine oil in liquid BHM medium. The best three isolates A4, B6 and D5 were identified and the optimal temperature and pH for biodegradation of spending engine oil were studied. Also, the consortium culture of three isolates was tested their ability to utilize spent engine oil under the same conditions for single isolate. Twenty five bacterial isolates were obtained from contaminated soil samples and three isolates appeared a maximum degradation rate 74.6, 70.2 and 78.5% respectively. The results from identification tests were showing these isolates belong to Bacillus sp., Acinetobacter sp. and Pseudomonas sp., respectively. The studied three isolates gave the best degradation when incubated at 30°C in BHM medium pH 7. While other results were indicated that consortium cultures are more effective 90.2% than all experiments that used single isolate.

scholarly journals Biodegradation of used Engine Oil Contaminated Site using Individual and Mixed bacterial isolates

2020 ◽  
pp. 38-45
Author(s):  
Muh'd A. J. ◽  
Sa'adatu A.Y. ◽  
Surayya M. M. ◽  
Sa'adatu M. I. ◽  
Nafisa B. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Contaminated Soil ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Contaminated Site ◽  
Bacterial Isolates ◽  
The World ◽  
Degradation Ability

Oil is most widely distributed source of energy in the world and large-scaled environmental pollutant. Oil, oil products, and oil containing industrial waste pollution is ranked second place after radioactive pollution on account of their harmful action to ecosystems. Contamination of soil by organic chemicals (mostly hydrocarbons) is prevalent in oil producing and industrialized countries of the world. Biodegradation, a strategy that uses biological means (i.e microbes) to degrade, stabilize and remove soil contaminants is an alternative green technology remediation of hydrocarbon contaminated soil. This study was carried out to isolate and screen Bacteria capable of degrading used engine oil from oil contaminated site. Soil samples were collected from oil contaminated site. Bacterial species were isolated from the collected soil samples by serial dilution and agar methods. Different bacterial species were isolated but only four were oil degrading isolates. The identity of the various genera of bacterial contaminants were determined by a combination of cultural, morphological as well as preliminary biochemical characteristics of the isolates. The four oil degrading bacteria (AJ1, AJ2, AJ3 and AJ4) were preliminarily identified as Bacillus cereus, Pseudomonas aeruginosa, Bacillus subtillis and Micrococcus spp respectively. The degradation ability of the bacterial isolates was screened and maximum degradation was recorded by AJ 5 (Mixed culture) with 66.9 %, followed by Bacillus cereus (50.3 %), Bacillus subtilis (44 %), Pseudomonas aeruginosa (37.9 %) and the least was seen in Micrococcus spp (35.3 %). These findings revealed that some bacteria species are capable of utilizing the oil and used it as sole source of carbon and energy and the mixed consortia of the bacteria have rapid degradation ability. Biological degradation of hydrocarbon contaminated soil offers a better and more environmentally friendly technique that if properly explored can bring our environment into a better place for both plant and animal.

scholarly journals Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India

2010 ◽  
Vol 2 (3) ◽  
pp. 559 ◽  
Author(s):  
H. Prasad V ◽  
G. Kumar ◽  
Kartik L ◽  
B. Rao KV
Keyword(s):  
Enzyme Activity ◽  
Tamil Nadu ◽  
Agar Medium ◽  
Agar Plate ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Keratinolytic Activity ◽  
Plating Method

The aim of the current study was to isolate keratinolytic bacteria from the soil samples collected from different feather processing areas in Vellore, TN, India. The isolation was performed by serial dilution and plating method. Total eight bacteria were isolated from the collected soil samples. All isolates were screened for keratinolytic activity by Casein agar plate method, among eight bacterial isolates only one (H5) isolate showed the keratinolytic activity in Casein agar medium. H5 isolate (potential strain) was identified as Bacillus sp. by microscopic and biochemical experiments. The best enzyme activity was observed at pH 7 and temperature 30ºC.  Keywords: Keratinolytic; Spread plate method; Bacillus sp.; Bacteria. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4567              J. Sci. Res. 2 (3), 559-565 (2010) 

scholarly journals Isolation and Characterization of Zinc Solubilizing Bacteria from Rhizosphere soil of Latur District, Marathwada, India

2019 ◽  
Vol 16 (04) ◽  
pp. 797-803
Author(s):  
Rajmahammad Rasul Tamboli
Keyword(s):  
Rhizosphere Soil ◽  
Agar Medium ◽  
Bacterial Species ◽  
Soil Samples ◽  
Growth And Yield ◽  
Wheat Crop ◽  
Zinc Carbonate ◽  
Liquid Salt ◽  
Isolation And Characterization

ABSTRACT: Rhizosphere soil samples from Wheat crop were collected from the 10 different locations in Latur district of Marathwada region with the objective to isolate the zinc solubilizing bacteria, their screening and characterization. Zinc carbonate was used as insoluble zinc source. Out of 10 Zn solubilizers, 3 most outstanding isolates were maintained for further screening for mineral solubilization (Zn and K). Among these RRT19, RRT34 and RRT13 which was identified as Bacillus cereus and Bacillus subtilis by morphological and biochemical test. These isolates showed maximum zone of solubilization with 34, 31 to 30 mm on liquid salt agar medium after 48 hrs of incubation respectively. The bacterial species isolated from the Rhizosphere soil can be use in soils that are deficient in Zinc or where insoluble zinc is abundant. The present study concluded that the use of zinc solubilising bacteria in the zinc deficient soil will help to enhance the growth and yield of wheat crop.

Sidrophore Production and Phosphate Solubilization by Bacillus cereus and Pseudomonas fluorescens Isolated from Iraqi Soils and Soil Characterization

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H
Keyword(s):  
Bacillus Cereus ◽  
Pseudomonas Fluorescens ◽  
Phosphate Solubilization ◽  
Bacterial Species ◽  
Nutrient Status ◽  
Bacterial Count ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Clear Zone ◽  
Zone Formation

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

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