scholarly journals Effect of sunflower oil supplementation in feed on body weight and hematobiochemical parameters in mice

2017 ◽  
Vol 28 (1) ◽  
pp. 36-41 ◽  
Author(s):  
A Basak ◽  
LA Banu ◽  
N Ahmad ◽  
K Rafiq

Sunflower oil contain high concentrations of essential polyunsaturated fat with antioxidant vitamin E. The study was carried out on Swiss Albino healthy mice to evaluate the effects of additional supplementation of sunflower oil in feed on body weight, hematological parameters (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins, low density lipoprotein and serum creatine). A total of 20, three-weeks old mice were randomly divided into two equal groups ( n=10) as A and B. Group A was considered as control (standard poultry pellet) and group B was supplemented with sunflower oil (5 mL/kg feed) respectively in addition to standard poultry pellet for 50 days. At the end of experimental trial, the mice were sacrificed for analysis of hemato-biochemical parameters. Body weight was significantly (P<0.05) higher in sunflower oil supplemented group compared to control. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B compared to control group A. The total serum cholesterol, HDL and LDL values increased significantly (P<0.05) whereas the triglycerides concentration decreased significantly (P<0.05) in sunflower oil treated group compared to control group. On the other hand serum creatinine value showed non-significant trend to increase (P<0.2). These results suggest that sunflower oil may have beneficial effects to maintain healthy life style. Although further studies are needed to find out the molecular mechanism and its toxicological aspect if any.Progressive Agriculture 28 (1): 36-41, 2017

2018 ◽  
Vol 5 (3) ◽  
pp. 365-372
Author(s):  
Nafis Islam ◽  
Md Shahidul Islam ◽  
Md Jahangir Alam ◽  
Md Kamrul Islam ◽  
Nazim Ahmad

The study was conducted on “Swiss Albino” mice fed with additional supplementation of soybean oil and rice bran oil to observe the effects on hematological (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins and uric acid). A total of 30, 6-8 weeks old mice were randomly divided into 3 equal groups (n=10) as A, B and C. Group A was considered as control (fed only commercial ration), group B was supplemented with rice bran oil and group C treated with soybean oil respectively in addition to commercial ration for 60 days. At the end of feeding trial the mice were sacrificed for analysis of hematobiochemical parameters. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B and C compared to control group A and the highest values was recorded in soybean oil group C. The total serum cholesterol, triglycerides, HDL and uric acid were increased significantly (P<0.05) in both rice bran oil and soybean oil group compared to control group. It is concluded that some hemato-biochemical parameters of blood in the mice are affected by rice bran and soybean oil enriched diet. Though, oils and fats are detrimental to health but to evaluate the effects of rice bran and soybean oil, further studies with more animals and some other parameters like liver function, kidney function may be conducted. Res. Agric., Livest. Fish.5(3): 365-372, December 2018


2013 ◽  
Vol 42 (2) ◽  
pp. 148-151
Author(s):  
M Salahuddin ◽  
M Sarker ◽  
N Ahmad ◽  
MA Miah

Plant fat like soybean oil is believed to be less harmful to our body compared to animal fat. To know the effect of soybean oil on hemato-biochemical profile, a total of 20, 6 weeks old “Swiss Albino” mice were randomly divided into 4 equal groups (n=5). Group A was considered as control fed with rat pellets and others were treated with 2% (group B), 4% (group C) and 8% (group D) soybean oil, respectively, in addition to pellets. At the end of 45 days, hemato-biochemical parameters were analyzed. The total erythrocyte count (TEC) and hemoglobin (Hb) content were increased significantly (P<0.05) in group C and D compared to control group A and the highest concentration was recorded in group D. The total serum cholesterol, triglycerides, HDL and uric acid were also increased significantly (p<0.01) in group C and D compared to control and highest value was recorded in group D. It is concluded that some haemato-biochemical parameters of blood in the mice are affected by soybean oil enriched diet. DOI: http://dx.doi.org/10.3329/bjas.v42i2.18502 Bang. J. Anim. Sci. 2013. 42 (2): 148-151


Author(s):  
MR Das ◽  
MMH Sarker ◽  
MB Rashid ◽  
MA Miah

To investigate the effect of exogenous enzymes, multivitamin and growth promoter on growth performance and different hemato-biochemical parameters of broilers, ten days old “Cobb 500” chicks were used and randomly divided into five equal groups (n=10). Group A was considered as control, fed only with commercial ration. Group B, C, D and E were supplemented with enzymes, multivitamin and enzymes plus multivitamin and growth promoter, respectively for the period of 21 days. Results revealed that body weight was increased significantly (p <0.05) in all four treated groups and highest body weight was recorded in group D. Weights of different organs except viscera (liver, spleen, skin, legs, breast meat, leg meat and viscera) were also varied significantly (p <0.05) among the treated groups. TEC increased significantly (p<0.05) in birds of all treated groups except group E whereas Hb concentration increased significantly (p<0.05) in all treated groups (B, C, D and E) as compared to control group A. Highest PCV was also recorded in group C but all the values were more or less similar and statistically insignificant (p>0.05). Biochemical parameters like AST were decreased significantly (p<0.05) in treated groups except group B and C. But, ALT values decreased significantly (p<0.05) in all treated groups in comparison to control group. It is concluded that combined supplementation of enzyme and multivitamin improved better in body weight and different organs weight gain and improves hemato-biochemical profile in broilers.International Journal of Natural Sciences (2014), 4(1) 1-4


2013 ◽  
Vol 30 (1) ◽  
pp. 20-24 ◽  
Author(s):  
M Salahuddin ◽  
H Akhter ◽  
S Akter ◽  
MA Miah ◽  
N Ahmad

Probiotic organisms are live microorganisms thought to be beneficial to the host. To know the effect of probiotic supplemented food on haemato-biochemical parameters in mice, a total of 20 two-month-old Swiss Albino mice were randomly divided into four equal groups. Group A was control, fed commercial mouse pellets, and others were treated with 5 g (group B), 10 g (group C) and 20 g (group D) probiotics (curd), respectively, in mouse pellets for 45 days. Total erythrocyte count and haemoglobin concentration were significantly (P<0.01) higher in treated group than in controls. Total serum cholesterol, triglyceride, high density lipoprotein and low density lipoprotein values decreased significantly (P<0.01) in treated groups. Serum uric acid value was significantly higher (P<0.01) in treated groups. It is suggested that probiotics help improve haematology and lipid profile but not kidney function. DOI: http://dx.doi.org/10.3329/bvet.v30i1.16281 Bangl. vet. 2013. Vol. 30, No. 1, 20-24


2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).


2018 ◽  
Vol 4 (2) ◽  
pp. 222-226
Author(s):  
Md Saidur Rahman ◽  
Md Mehedi Hasan ◽  
Md Ehsanul Kabir ◽  
Nahid Nawrin Sultana ◽  
Md Eliusur Rahman Bhuiyan ◽  
...  

The experiment was conducted to compare the effects of animal fat (ghee) and vegetable fat (dalda) in respect to hemato-biochemical changes in mice. A total of twenty one, 45 days old (Swiss Albino) mice were randomly divided into three equal experimental groups as A, B and C (n=7). Group A was considered as control and fed only broiler pellet, group B was fed broiler pellet with ghee (25 gm/1000 gm feed) and group C was fed broiler pellet with dalda (25 gm/1000 gm feed) respectively. At day 40, all mice were sacrificed to collect blood for hematological parameters where blood profile was analyzed by biochemical tests. Total erythrocyte counts were significantly (p<0.05) higher in ghee and dalda supplemented mice (B: 8.99±.34 million/cu. mm. and C: 8.27±.43 million/cu.mm.) compared to control group (A: 7.49±.22 million/cu.mm.). The significantly (P<0.05) higher hemoglobin concentration was recorded in ghee supplemented group B (9.02±.18 gm/dl) than other groups and control group A showed lowest value (7.59±.52 gm/dl). In Blood profile analysis, serum cholesterol and HDL value were higher in ghee and dalda supplemented group than control group. On the other hand triglyceride value was significantly (P<0.05) lower in control group (A: 44±4.58 mg/dl) than ghee and dalda supplemented group (B: 80.33±2.51 mg/dl, C: 71.2±4.26 mg/dl). The study suggests that ghee and dalda supplementation with regular diet would be able to significantly increase cholesterol and triglyceride in mice.Asian J. Med. Biol. Res. June 2018, 4(2): 222-226


2016 ◽  
Vol 14 (1) ◽  
pp. 37-42 ◽  
Author(s):  
M. G. Sorwar ◽  
M. Mostofa ◽  
M. N. Hasan ◽  
M. Billah ◽  
M. T. Rahman

This experiment was conducted to determine the effect of papaya leaf (Carica papaya) and kalo jeera (Nigella sativa) seeds powdered supplementation in drinking water as a growth promoter in broiler chickens. A total of 20 Cobb-500 broiler chicks (day-old) were purchased from local hatchery (Nourish Poultry and Hatchery Ltd.) and after seven days of acclimatization chicks were randomly divided into two groups, A (n=10) and B (n=10). The group A was kept as a control and not treated. The group B was supplemented with papaya leaf and kalo jeera powder with feed and water. Weekly observations were recorded for live body weight gain up to 5th weeks and hematological tests were performed at 35th day’s age of broiler to search for hematological changes between control (A) and treatment (B) groups. The initial body weight of groups A and B on 1st were 41.00±0.56 gm and 41.50±0.35 gm, respectively and after 35th day of experiment final body weight were 1470±57.35 gm and 1720±58.56 gm, respectively and economics of production were analyzed and found that net profit per broiler was Tk. 8.91 and Tk. 20.69, respectively. The treatment group B was recorded statistically significant (at 1% level) increased (17.00%) for live body weight than that of control group A. The hematological parameters total erythrocyte count (TEC), erythrocyte sedimentation rate (ESR) and hemoglobin (Hb) estimation value of treatment group shows significant difference, while hemoglobin estimation does not show significant difference from control group. The results suggest that better growth performance could be achieved in broilers supplemented with papaya leaf and kalo jeera seeds.


Author(s):  
Jayendra R. Gohil ◽  
Vishal S. Rathod ◽  
Bhoomika D. Rathod

Objective: To study the effect and safety of Fenofibrate in uncomplicated hyperbilirubinemia in newborn with 6-month follow-up. Materials and Methods: This is a randomized controlled clinical trial conducted in 60 normal term neonates admitted for uncomplicated hyperbilirubinemia in NICU at Sir T G Hospital, Bhavnagar from January 2012 to December 2012. The data included: age, sex, total serum bilirubin (TSB), weight and duration of phototherapy. All neonates enrolled in the study received phototherapy. They were divided in two groups of 30 each: control group A and group B receiving Fenofibrate (100 mg/kg single dose). There was statistically insignificant difference between the parameters of age, sex, weight and TSB between the two groups at hospitalization. Data was analyzed by using appropriate statistical methods. Results: Mean values for total serum bilirubin in Fenofibrate group B at 24 and 48 hours after admission were significantly lower than those for control group A (p<0.0001,  p=0.0001). There was no significant difference in fall of TSB between 24 and 48 hours. The mean duration of phototherapy in Fenofibrate group (44.8h: 24-72h) was significantly shorter than that in control group (55.2 h: 24‐96 h) (P=0.02). There were no side effects of the drug observed during the study and during 6 months follow up period. Conclusion: Fenofibrate as a single 100 mg/kg dose in healthy full term neonates, is effective and a safe drug (till six-month follow-up) for neonatal hyperbilirubinemia, that can decrease the time needed for phototherapy and hence hospitalization. Effect of a single dose seems to wane after 24 hours.


Author(s):  
Karan Tikate ◽  
M. Wade ◽  
A. S. Ranade ◽  
V. R. Patodkar ◽  
V. S. Dhaygude ◽  
...  

The experimental trial was carried out to study the effect of multiple phase feeding on the growth performances and the economics of broiler production for a period of six weeks. Day-old chicks (n=160) were divided into two treatment groups, each containing 80 chicks with four replicates in each treatment. The birds of the control group (A) were fed prestarter, starter and finisher diets as per nutrient requirement of commercial broiler strain used in the experiment and the multiple phase feeding was followed in group-B by changing the nutrient densities (Energy, Crude protein, lysine and methionine) of diet per week. The different performance parameters were studied such as live body weight, gain in live body weight, feed conversion ratio and feed consumption at weekly interval. Mortality was recorded daily and the economics was calculated at the end of experiment. The results of experiment showed that the body weight and weight gain of broilers of group-B was significantly higher (p less than 0.05) than that of broilers of group-A during first, second, fifth and sixth week of age and also during overall duration of the experiment. Further, the feed intake of broilers of group-B was significantly higher (p less than 0.05) than that of group-A during second, fifth and sixth weeks of age and also during the overall feed intake of broilers. The Feed Conversion Ratio of broilers of group-B was significantly better (p less than 0.05) than that of group-A during fifth week and also during the overall performance. The multiple phase feeding has not put any adverse effect on percentage mortality of broilers. However, broilers fed with the multiple phase feeding showed higher net profit per bird and also per kg of body weight as compared to the broilers of control group (A).


2015 ◽  
Vol 115 (1) ◽  
pp. 1-5 ◽  
Author(s):  
L. Alonso ◽  
J. Fontecha ◽  
P. Cuesta

AbstractA total of twenty-four Yorkshire gilt pigs of 6–7 weeks of age were used in a 2×2 factorial experiment to determine the individual and combined effects of the inclusion of two dietary factors (cholesterol rich, 3 %β-cyclodextrin (BCD) andLactobacillus acidophiluscultures) on total cholesterol and LDL-cholesterol levels in blood serum. Pigs were assigned randomly to treatment groups (n6). Total serum cholesterol concentrations decreased after 3 weeks in all the experimental treatment groups, including diets with BCD,L.acidophilusor both. Similar trends were observed for serum LDL-cholesterol concentrations among the experimental treatments. No statistically significant differences from the control group were observed in either total serum cholesterol or LDL-cholesterol concentrations (P<0·05) for each of the individual treatment groups: BCD orL.acidophilus. However, significant differences in total serum cholesterol concentrations were observed when comparing the combined treatment group (BCD andL.acidophilus) with the control group, which consisted of a basal diet and sterile milk. The combined treatment group exhibited 17·9 % lower total serum cholesterol concentration after 3 weeks. Similar significant differences were observed when comparing the combined effect experimental group with the control group after 3 weeks. The combined treatment group exhibited 27·9 % lower serum LDL-cholesterol concentrations.


Sign in / Sign up

Export Citation Format

Share Document