scholarly journals Comparison the effects of animal fat (ghee) with that of vegetable fat (dalda) in respect to hemato-biochemical parameters in mice

2018 ◽  
Vol 4 (2) ◽  
pp. 222-226
Author(s):  
Md Saidur Rahman ◽  
Md Mehedi Hasan ◽  
Md Ehsanul Kabir ◽  
Nahid Nawrin Sultana ◽  
Md Eliusur Rahman Bhuiyan ◽  
...  
Keyword(s):  
Profile Analysis ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Biochemical Tests ◽  
Blood Profile ◽  
Animal Fat ◽  
Group A ◽  
Group B ◽  
Vegetable Fat

The experiment was conducted to compare the effects of animal fat (ghee) and vegetable fat (dalda) in respect to hemato-biochemical changes in mice. A total of twenty one, 45 days old (Swiss Albino) mice were randomly divided into three equal experimental groups as A, B and C (n=7). Group A was considered as control and fed only broiler pellet, group B was fed broiler pellet with ghee (25 gm/1000 gm feed) and group C was fed broiler pellet with dalda (25 gm/1000 gm feed) respectively. At day 40, all mice were sacrificed to collect blood for hematological parameters where blood profile was analyzed by biochemical tests. Total erythrocyte counts were significantly (p<0.05) higher in ghee and dalda supplemented mice (B: 8.99±.34 million/cu. mm. and C: 8.27±.43 million/cu.mm.) compared to control group (A: 7.49±.22 million/cu.mm.). The significantly (P<0.05) higher hemoglobin concentration was recorded in ghee supplemented group B (9.02±.18 gm/dl) than other groups and control group A showed lowest value (7.59±.52 gm/dl). In Blood profile analysis, serum cholesterol and HDL value were higher in ghee and dalda supplemented group than control group. On the other hand triglyceride value was significantly (P<0.05) lower in control group (A: 44±4.58 mg/dl) than ghee and dalda supplemented group (B: 80.33±2.51 mg/dl, C: 71.2±4.26 mg/dl). The study suggests that ghee and dalda supplementation with regular diet would be able to significantly increase cholesterol and triglyceride in mice.Asian J. Med. Biol. Res. June 2018, 4(2): 222-226

scholarly journals Effects of soybean and rice bran oil on hemato-biochemical parameters in mice

2018 ◽  
Vol 5 (3) ◽  
pp. 365-372
Author(s):  
Nafis Islam ◽  
Md Shahidul Islam ◽  
Md Jahangir Alam ◽  
Md Kamrul Islam ◽  
Nazim Ahmad
Keyword(s):  
Soybean Oil ◽  
Rice Bran ◽  
Biochemical Parameters ◽  
Rice Bran Oil ◽  
Hemoglobin Concentration ◽  
Total Serum ◽  
Control Group ◽  
Total Serum Cholesterol ◽  
Group A ◽  
Group B

The study was conducted on “Swiss Albino” mice fed with additional supplementation of soybean oil and rice bran oil to observe the effects on hematological (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins and uric acid). A total of 30, 6-8 weeks old mice were randomly divided into 3 equal groups (n=10) as A, B and C. Group A was considered as control (fed only commercial ration), group B was supplemented with rice bran oil and group C treated with soybean oil respectively in addition to commercial ration for 60 days. At the end of feeding trial the mice were sacrificed for analysis of hematobiochemical parameters. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B and C compared to control group A and the highest values was recorded in soybean oil group C. The total serum cholesterol, triglycerides, HDL and uric acid were increased significantly (P<0.05) in both rice bran oil and soybean oil group compared to control group. It is concluded that some hemato-biochemical parameters of blood in the mice are affected by rice bran and soybean oil enriched diet. Though, oils and fats are detrimental to health but to evaluate the effects of rice bran and soybean oil, further studies with more animals and some other parameters like liver function, kidney function may be conducted. Res. Agric., Livest. Fish.5(3): 365-372, December 2018

scholarly journals Effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate, alum and effects of sand-charcoal-iron-filter bed filtrated water along with alum on body weight and some hematological parameters in rabbit

2016 ◽  
Vol 2 (1) ◽  
pp. 38-47
Author(s):  
Shaifuddin Ahmed ◽  
Md Saiful Islam Siddiqui ◽  
Kamrul Islam ◽  
Mohammad Usman Gani ◽  
Sharifunnessa Moonmoon ◽  
...  
Keyword(s):  
Body Weight ◽  
Arsenic Trioxide ◽  
Tannic Acid ◽  
Hematological Parameters ◽  
Control Group ◽  
Hydrogen Phosphate ◽  
Sodium Hydrogen Phosphate ◽  
Sodium Hydrogen ◽  
Group A ◽  
Group B

The effect of arsenic trioxide along with tannic acid, di-sodium hydrogen phosphate (DSHP), alum and effects of sand-charcoal-iron-filter (SCIF)-bed filtrated water along with alum on body weight and some hematological parameters were carried out on a total of 30 (01 month old) adult Newzealand white rabbits. The Rabbits were randomly divided in to 6 equal groups (A,B,C,D,E & F) at the ratio of three males and two females in each group, rats of group A was kept as control without giving any treatment, rabbits of group B received arsenic trioxide@100 ppm, group C received arsenic trioxide @ 100 ppm plus tannic acid @100 ppm, group D received arsenic trioxide @ 100 ppm plus di-sodium hydrogen phosphate @100 ppm, group E received arsenic trioxide @100 ppm plus alum @100 ppm orally daily for 60 days in all cases and group F received alum @100 ppm in SCIF-bed filtrated water orally daily for 60 days. The result showed that body weight gain of control group (A) per week per rabbit was found to increase but in treated group B (arsenic trioxide@100ppm orally daily) the mean body weight of group B were decreased gradually and significant (P<0.05) decrease in body weight was observed at 40 days of feeding and at day 60 it was highly significant (P<0.01). Rabbits of group C, D and E were apparently normal and mild body weight reductions were observed which was statistically insignificant. No body weight loss was observed in rabbit of group F. TEC, Hb and PCV were slightly reduced in group C, D, E and F but highly significant (P< 0.01) reduction of TEC, Hb and PCV were observed in group B. ESR values were slightly increased in group C, D, E and F but highly significant (P< 0.01) increased of ESR value was observed in group B. Leukocytosis was observed in rabbits of group B. In this study, it was concluded that treatment with arsenic oxide at low doses has harmful effects on experimental animals including hematological alterations.Asian J. Med. Biol. Res. March 2016, 2(1): 38-47

scholarly journals Effect of sunflower oil supplementation in feed on body weight and hematobiochemical parameters in mice

2017 ◽  
Vol 28 (1) ◽  
pp. 36-41 ◽  
Author(s):  
A Basak ◽  
LA Banu ◽  
N Ahmad ◽  
K Rafiq
Keyword(s):  
Body Weight ◽  
Serum Cholesterol ◽  
Sunflower Oil ◽  
Biochemical Parameters ◽  
Hemoglobin Concentration ◽  
Total Serum ◽  
Control Group ◽  
Total Serum Cholesterol ◽  
Group A ◽  
Group B

Sunflower oil contain high concentrations of essential polyunsaturated fat with antioxidant vitamin E. The study was carried out on Swiss Albino healthy mice to evaluate the effects of additional supplementation of sunflower oil in feed on body weight, hematological parameters (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins, low density lipoprotein and serum creatine). A total of 20, three-weeks old mice were randomly divided into two equal groups ( n=10) as A and B. Group A was considered as control (standard poultry pellet) and group B was supplemented with sunflower oil (5 mL/kg feed) respectively in addition to standard poultry pellet for 50 days. At the end of experimental trial, the mice were sacrificed for analysis of hemato-biochemical parameters. Body weight was significantly (P<0.05) higher in sunflower oil supplemented group compared to control. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B compared to control group A. The total serum cholesterol, HDL and LDL values increased significantly (P<0.05) whereas the triglycerides concentration decreased significantly (P<0.05) in sunflower oil treated group compared to control group. On the other hand serum creatinine value showed non-significant trend to increase (P<0.2). These results suggest that sunflower oil may have beneficial effects to maintain healthy life style. Although further studies are needed to find out the molecular mechanism and its toxicological aspect if any.Progressive Agriculture 28 (1): 36-41, 2017

scholarly journals Effect of Physical Exercise on some Hematological Parameters in Female Athletes in Bangladesh

1970 ◽  
Vol 52 (195) ◽  
pp. 892-896 ◽  
Author(s):  
Tanbira Alam ◽  
Lubna Shirin ◽  
SM Niazur Rahman ◽  
Tanjeda Alam ◽  
Nasrin Habib ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Serum Iron ◽  
Female Athletes ◽  
Ferritin Level ◽  
Hematological Parameters ◽  
Control Group ◽  
Group A ◽  
Group B

Introduction:  Long term physical training has been considered to adversely affect the performance of athletes especially the females. It may be due to the iron depletion caused by hemolysis or hemodilution results from plasma volume expansion. This study aims to assess the effect of heavy exercise on hemoglobin concentration and some other hematological parameters in female athletes. Methods: This cross sectional study was carried out in the Department of physiology, Dhaka Medical College, Dhaka, from July 2010-June 2011. A total number of 105 females (70 athletes and 35 non athletes) between 15-25 years of age were recruited. The 35 apparently healthy female non athletes were taken as control (Group-A) and 70 female athletes comprise the study group (Group-B). According to sporting category, Group-B was further subdivided as B1:Runners and B2:Cyclists. Hemoglobin percentages (Hb %), red blood cell (RBC) concentration, packed cell volume (PCV), serum iron, serum ferritin and total iron binding capacity (TIBC) were measured in fasting state after two months training. Statistical analysis was done by using SPSS/ win.15.0 for comparison between the groups, using student’s unpaired “t” test. Results: Mean Hb%, RBC, PCV, iron and ferritin level were significantly (P<0.001) lower in athletes than those of control group. Within the study groups, runner’s serum iron and ferritin level were lower than cyclists.  TIBC significantly (P<0.001) rose in Group-B2 in comparison to Group-A. Conclusions: The current study showed that intense physical exercise leads to early stages of Hb%, RBC, PCV, iron and ferritin depletion which might compromise the health and performance of athletes. Keywords: exercise; hemoglobin; packed cell volume; serum iron.  

scholarly journals Effects of colistin sulfate on hematological parameters in broiler

2021 ◽  
Vol 7 (2) ◽  
pp. 113-117
Author(s):  
Md Nazmul Hasan ◽  
Md Shafiqul Islam ◽  
Md Rakibul Hasan ◽  
Kazi Rafiqul Islam
Keyword(s):  
Hematological Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Withdrawal Period ◽  
Colistin Sulfate ◽  
Significant Difference ◽  
Antibiotic Group ◽  
Group A ◽  
Group B

Hematological indication is an important finding for pathophysiological analysis of biological science. This study was designed with the aim to investigate the effects of residual antibiotics on hematological parameters of broiler following discriminate and indiscriminate use. The day old broiler chicks were collected and reared up to 31 days. The treatment was started from the day 16th until sacrifice. The chicks were randomly divided into three groups namely control group (Group A), discriminate antibiotic group (Group B) and indiscriminate antibiotic group (Group C) on the 14th day. The discriminate group was treated with antibiotic, colistin sulfate maintaining the withdrawal period of one week. In case of indiscriminate group the withdrawal period was not maintained and antibiotic treatment was continued till the day of sacrifice. The Hb (%) of control, discriminate and indiscriminate groups were 8.34±0.15, 6.89±0.27, and 7.14±0.23 respectively. The differences among means were statistically significant (P<0.05). The Packed Cell Volume (PCV) of control, discriminate, and indiscriminate groups were 23.51±0.76, 21.17±0.94, and 22.83±1.19 respectively. The differences among means were statistically significant. The multiple pair wise comparison of means of blood parameters revealed that there was no significant difference among discriminate and indiscriminate groups. From the above findings, this research could be considered a need based research in Bangladesh to ascertain the influential effect of antibiotic on hematological parameters in broiler. Asian J. Med. Biol. Res. 2021, 7 (2), 113-117

scholarly journals Effects of bisphenol-A (BPA) on body weight, hematological parameters and histo-texture of kidney in swiss albino mice

2021 ◽  
Vol 6 (4) ◽  
pp. 635-640
Author(s):  
Emdadul Hoque ◽  
Khaled Mahmud Sujan ◽  
Md Suman Mia ◽  
Md Iqramul Haque ◽  
Afrina Mustari ◽  
...  
Keyword(s):  
Body Weight ◽  
Bisphenol A ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
The Body ◽  
High Dose ◽  
Daily Lives ◽  
Group A ◽  
Group B

Bisphenol-A (BPA) is one of the highest volume chemicals produced world-wide and used in the manufacture of plastics and epoxy resins that are pervasive in our environment and daily lives. The present research was carried out to investigate the effects of two different doses of Bisphenol-A (BPA) on the body weight, hematological parameters and patho-physiological changes of kidney in mice. For this study, fifteen mice, 6 to 8 weeks of age with an average bwt 27.10±0.5 gm, were randomly divided into three groups (n= 5). Group A (control) received only normal mouse pellet while group B and group C received pellet mixed with BPA @ 50 mg and 100 mg / kg bwt daily for 12 weeks, respectively. At the end of the experiment, blood and tissues were collected and processed for hematological and histopathological examination. Results showed that BPA- treated mice caused significant elevation (p<0.01) in weight gain even treated with low dose (50mg) of BPA. The mice exposed to high dose of BPA (100 mg) showed marked reduction (p<0.05) in total erythrocyte count (TEC), significant decreased (p<0.01) in hemoglobin concentration (Hb) and packed cell volume (PCV). Histopathological alterations were detected in the kidneys of BPA-treated mice. In conclusion, this study suggested that BPA exerts deleterious impacts on hematological parameters including association with renal injuries. Asian J. Med. Biol. Res. December 2020, 6(4): 635-640

scholarly journals INVESTIGATION ON THE COUNTERACTING EFFECT OF SPIRULINA AGAINST POTENTATED SULFONAMIDE’S (COTRIM DS®) SIDE EFFECTS IN RAT

2013 ◽  
Vol 10 (1-2) ◽  
pp. 81-86 ◽  
Author(s):  
M. K. Islam ◽  
S. Akter ◽  
S. Bala ◽  
M. Z. Hossain ◽  
M.S. Akter
Keyword(s):  
Hematological Parameters ◽  
Experimental Period ◽  
Treated Group ◽  
Control Group ◽  
High Dose ◽  
Spirulina Maxima ◽  
Liver And Kidney ◽  
Group A ◽  
Group B ◽  
And Control

An experiment was conducted to investigate the counteracting effects of spirulina in Long Evans rats exposed to oral potentiated sulfonamide administration. 20 rats were randomly assigned into four equal groups (A, B, C and D) and were fed with standard broiler pellet (25g/rat/day) throughout the experimental period of 60 days. Rats of Group A were fed only with pellet without any experimental diet and were defined as control. Rats  of Group B were treated with potentiated sulfonamide @ 96 mg/rat/day orally whereas Group C was treated with potentiated sulfonamide @ 96 mg/rat/day plus spirulina (Spirulina maxima) @ 50 mg/rat/day orally (low dose spirulina). In Group D, potentiated sulfonamide and spirulina (Spirulina maxima) were given through feed @ 96 mg/rat/day and @ 100 mg/rat/day (high dose spirulina) respectively. Hematological parameters (TEC, Hb and absolute count of lymphocyte, neutrophil and eosinophil) and hispathological profile of liver and kidney were recorded. The investigation revealed that the oral administration of sulfonamide significantly (p<0.01) decreased the TEC (5.93±0.24) value, number of lymphocyte (581.76±3.70) and neutrophil (581.76±3.70) compared to other treated groups and control group. On the other hand significant (p<0.01) increase (422.86±2.34) in eosinophil population has been found in rats fed on sulfonamide irrespective of spirulina supplementation on the final day of experiment compared to other treated group and control group. From this experiment it is evidenced that spirulina has a potential counteracting effect against sulfonamide. Histopathology of kidney and liver was done at the end of experiment (60 days) and no significant change was found except in the kidney of Group B and C.DOI: http://dx.doi.org/10.3329/bjvm.v10i1-2.15650

scholarly journals Effects of discriminate and indiscriminate use of enrofloxcin on hematological parameters

2021 ◽  
Vol 7 (1) ◽  
pp. 09-015
Author(s):  
Md. Mominul Islam Bhuiyan ◽  
Md. Shafiqul Islam ◽  
Md. Rakibul Hasan ◽  
Kazi Rafiqul Isla
Keyword(s):  
Packed Cell Volume ◽  
Hematological Parameters ◽  
The Other ◽  
Control Group ◽  
Blood Parameter ◽  
Withdrawal Period ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Control Group Group

Hematological indication is an important finding for pathophysiology analysis of biological science. This study was designed with the aim to investigate the effects of residual antibiotics on hematological parameters of broiler following discriminate and indiscriminate use. 18 broiler DOC (Cobb-500) were reared up to 31 days. On day 16, they were randomly divided into 3 groups, namely Group–A (Control group), Group-B (Discriminate group) and Group-C (Indiscriminate group). Each group contains 6 birds. The discriminate and indiscriminate group was treated with antibiotic, enrofloxacin. In Group-B withdrawal period was followed and treatment was stopped before 7 days of sacrifice. On the other hand, withdrawal period was not maintained in indiscriminate group and the antibiotic treatment was continued until the day of sacrifice. The Total erythrocyte count (million/mm3) of control, discriminate, and indiscriminate groups were 3.15±0.047, 2.66±0.091, and 2.90±0.11 respectively. The Hb (gm%) of control, discriminate, and indiscriminate groups were 8.17±0.07, 7.37±0.09, and 7.28±0.07 respectively. The Packed Cell Volume (%) of control, discriminate, and indiscriminate group were 24.17±0.48, 23.17±0.48, and 22.33±0.67 respectively. There was no significant difference on blood parameter found among the groups. Therefore, discriminate and indiscriminate use of enrofloxacin has no bad effect on hematological parameters of broiler.

scholarly journals Effects of aqueous extract of Neem (Azadirachta indica) leaves as growth promoter and anti-colibacillosis in broilers

2014 ◽  
Vol 43 (2) ◽  
pp. 138-141 ◽  
Author(s):  
SK Sarker ◽  
M Mostofa ◽  
F Akter ◽  
MM Rahman ◽  
MR Sultana
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Body Weight Gain ◽  
Hematological Parameters ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Neem Leaves ◽  
Group A ◽  
Group B

The study was conducted to determine the efficacy of aqueous extract of Neem leaves against colibacillosis and as a growth promoter in broilers. A total of 40 commercial day-old broiler chicks were randomly divided into four equal groups; viz. A, B, C and D. Group A was kept as non-treated control, Group B  and C was treated with 1% Neem leaves in drinking water for six weeks,  and colibacillosis was induced at 2nd week in group C and  D. In group D, Neem leaves treatment continued from 2nd to 6th week after the colibacillosis induction to compare its antibacterial efficacy to prophylactic effect. Escherichia coli induction rate was 200µl per bird where 1 ml contains approximately 1X106 CFU (Colony Forming Unit). Data were recorded for live body weight, weekly gain in weight and hematological parameters of birds for six weeks. Clinical examination and antibacterial sensitivity studies  suggests administration of aqueous extract of Neem leaves significantly (p<0.001) improved body weight gain in the Neem treated groups but did not prevent E. coli induced colibacillosis in broilers.DOI: http://dx.doi.org/10.3329/bjas.v43i2.20715 Bang. J. Anim. Sci. 2014. 43 (2): 138-141

scholarly journals Effects of triclabendazole and nitroxynil on EPG, hematological parameters and body weight against fascioliasis in goats at government goat development farm, Sylhet, Bangladesh

2016 ◽  
Vol 5 (2) ◽  
pp. 46-51 ◽  
Author(s):  
S Khanam ◽  
MS Islam ◽  
M Aktaruzzaman ◽  
MA Hossain ◽  
MM Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Dose Rate ◽  
Hematological Parameters ◽  
Experimental Period ◽  
The Body ◽  
Control Group ◽  
Infected Control Group ◽  
Group A ◽  
Group B ◽  
And Control

The study was conducted to evaluate the effects of triclabendazole and nitroxynil against fascioliasis in goat for a period of 28 days on 20 black bengal goats. Among hundred goats, twenty were selected for this study which was suffering from liver fluke (Fasciola gigantica) infection and were marked by tagging. Twenty goats were randomly divided into four groups (A, B, C and D) where each group comprising of five goats. Group A were treated with tablet fasinex (triclabendazole, Novartis Ltd, Bangladesh) orally at the dose rate of 12 mgkg-1body weight, group B were treated with acinex (triclabendazole, ACI Ltd, Bangladesh) orally at the dose rate of 12 mgkg-1body weight and group C were treated with nitronex injection (nitroxynil, Renata Ltd, Bangladesh) subcutaneously at the dose rate of 10 mgkg-1 body weight. Goats of group D was kept as infected control group without giving any treatment. A significant (P<0.01) reduction of egg per gram (EPG) count was found on 7th, 14th, 21st and 28th days of treated goats of group A, B and C, respectively. The EPG count of control group were significantly (P<0.01) increased 7 day onwards up to experimental period. Reduction of mean EPG on 7th, 14th, 21st and 28th day after treatment in group A, B and C were 81.48%, 81.25% and 83.04%, respectively, whereas in control group the mean EPG were 22.43%. Of the hematological parameters, TEC, Hb and PCV values were lower on day 0 but turned to increase (p<0.01) on day 28 of the study. On the other hand, ESR and TLC were higher before treatment (day 0) but decreased significantly (p<0.01) on day 28.The body weight increased significantly (P<0.01) after treatments in group A, B and C respectively except untreated control. This result may indicate that three commercial anthelmintics contain triclabendazole and nitroxynil against fascioliasis in goat and nitroxynil have higher efficacies on egg per gram (EPG), hematological parameters and body weight against fascioliasis in goat. Additional detailed studies are required to prevent and control of fascioliasis in goats by using a routine prophylactic anthelmintics measurement.International Journal of Natural Sciences (2015), 5(2) 46-51

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