scholarly journals Screening and isolation of arsenic tolerant Rhizobacteria from arsenic contaminated areas of Bangladesh

2019 ◽  
Vol 30 ◽  
pp. 17-25
Author(s):  
AM Taher ◽  
QF Quadir ◽  
MZ Hossen ◽  
T Nazneen ◽  
A Rahman
Keyword(s):  
Plant Species ◽  
Agar Medium ◽  
Plant Roots ◽  
Bacterial Isolates ◽  
Biochemical Traits ◽  
Nutrient Agar Medium ◽  
Pure Cultures ◽  
Maximum Tolerance ◽  
Solubilizing Capacity ◽  
Selection Of

An experiment was carried out to collect and isolate arsenic (As) tolerant rhizobacteria from As contaminated areas of Faridpur district. A total of 32 root samples of 17 plant species were collected from six locations of Bhanga and Nagarkanda upazila. From the plant roots, 16 rhizobacterial isolates were separated and their pure cultures were prepared. The collected rhizobacterial isolates were then characterized for morphological and biochemical traits. Among the bacterial isolates four were gram negative and 12 were gram positive. About 12 bacterial isolates had catalase producing capacity and the remaining three were negative to catalase test. Four bacterial isolates gave the positive HCN test. These bacterial isolates were grown on Pikovskays media to determine their phosphorus (P) solubilizing capacity. Four bacterial isolates had P solubilizing capacity and their Phosphorus Solubilizing Index (PSI) ranged between 3.00 and 4.66. However, isolate FB4 gave the highest P solubilization (4.66 PSI). For the evaluation of As tolerance, nutrient agar medium was prepared with different concentrations of As ranging from 30 to 600 ppm and the bacterial isolates were allowed to grow on the As contaminated medium for 36 hours. Isolate FB9 and FB4 showed maximum tolerance against As upto 600 ppm. Hence, these two isolates, both collected from Bhanga upazila and from wild brinjal species (Solanum capsicoides). Further research is needed to assess their effect on As dynamics at the rhizosphere, selection of suitable plant species for bacterial association and their potentials for field applications. Progressive Agriculture, Vol. 30, Suppl. 1: 17-25, 2019

scholarly journals The Effectivity of Bacteria Isolated From of Liquid Waste Palm Oil Plantation on Ganoderma Boninense

2019 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Fitratul Aini
Keyword(s):  
Palm Oil ◽  
Oil Palm ◽  
Root Rot ◽  
Agar Medium ◽  
Pathogenic Fungus ◽  
Liquid Waste ◽  
Ganoderma Boninense ◽  
Bacterial Isolates ◽  
Basal Stem Rot ◽  
Nutrient Agar Medium

Ganoderma boninense is one of the main pathogenic fungus in oil palm plantations. Generally, these pathogen cause root rot (basal stem rot). Biological control that has been widely used reduce the infection is using bacteria. Liquid waste palm oil has potential to produce bacteria that is able to degrade Ganoderma boninense that causes root rot in oil palm. Liquid waste were obtained from Muaro Sabak Regency Jambi Province. Bacteri were isolated and cultivated in nutrient agar medium, characterized and identified for antagonistic test against G. boninense. Results showed that 16 bacterial isolates were identified, among of them are able to inhibit Ganoderma boninense.

scholarly journals Study of Microbiological Quality of Biscuits from Street Side Bakery Shops at Dehradun City, India

2020 ◽  
pp. 319-326
Author(s):  
Irtiqa Syed ◽  
Rajendra Prasad ◽  
Adeeba Naaz
Keyword(s):  
Agar Medium ◽  
Microbiological Quality ◽  
Nutrient Agar ◽  
Morphological Observation ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Nutrient Agar Medium

<p>In the present study, microbiological quality of biscuits from street side bakery shops at Dehardun city, India was conducted. A total 11 samples of bakery biscuits were collected randomly and analysed for their microbiologically quality by standard plate count method. Pure cultures of bacterial isolates were prepared by streaking on nutrient agar medium. Bacterial isolates were further studied for morphological characters, culture characters on nutrient agar medium and biochemical testes in laboratory. All samples studies were found contaminated by a variety of bacteria. The highest bacterial load was 2.2 ×105 cfu/g and lowest was 0.1×103 cfu/g, respectively. Morphological observation, culture characters and results of biochemical tests of bacterial isolates were compared with standard results of known bacteria. The bacterial isolated were identified as Alcaligenes faecalis, Bacillus cereus, Micrococcus luteus, Pseudomonas aeruginosa, Streptococcus lactis and Staphylococcus aureus, respectively. Among these, two isolates i.e., A. faecalis and P. aeruginosa were gram negative and other isolates were gram positive bacteria which suggested the poor hygienic conditions inside the bakery during preparation and storage of biscuits. Gram positive bacteria might be contaminated during storage and by bad handling. These food spoiling bacteria might be responsible for food-borne infection and diseases. Therefore, care should be taken while purchasing such products and should be purchased from certified bakery shop.</p>

scholarly journals Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura

2018 ◽  
Vol 6 (2) ◽  
pp. 47-52
Author(s):  
Suprapto Surapto ◽  
Tri Gunaedi ◽  
Basa T. Rumahorbo
Keyword(s):  
Enzyme Activity ◽  
Agar Medium ◽  
Amylase Activity ◽  
Soluble Starch ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Crude Enzyme Extract ◽  
Nutrient Agar Medium ◽  
Bacillus Subtilis Atcc

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and  its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all  isolates were quite high: which were 35 577, 18 903,  32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.

scholarly journals CORRELATION BETWEEN CLASSROOM POPULATION, VENTILATION BACTERIAL LOADS AND THIER ANTIMICROBIAL PATTERNS IN SCHOOLS WITHIN IKOT EKPENE, NIGERIA

2019 ◽  
Vol 2 (4) ◽  
pp. 86 ◽  
Author(s):  
Aniefon Alphonsus Ibuot ◽  
Iniobong Ime James ◽  
Nsikan Samuel Udoekong ◽  
Mayen Ben ◽  
Christiana Utibe Etuk ◽  
...  
Keyword(s):  
Indoor Air ◽  
Agar Medium ◽  
Health Condition ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Ambient Conditions ◽  
Nutrient Agar Medium ◽  
Plate Counts ◽  
Aerobic Plate Count ◽  
Negative Relationships

Indoor air of classroom in eight schools (4 nursery; NS1, NS2, NS3 and NS4, and 4 secondaries; SS1, SS2, SS3 and SS4) within Ikot Ekpene, Akwa Ibom State, Nigeria, were analyzed at ambient and populated sampling conditions using natural sedimentation on nutrient agar medium. The results revealed varying ventilation patterns in each of the classrooms, and the following airborne bacterial counts; NS1 (16.6 cfu/m3), NS2 (13.3 cfu/m3), NS3 (23.3 cfu/m3), NS4 (33.3 cfu/m3), SS1 (6.6 cfu/m3), SS2 (6.6 cfu/m3), SS3(28.3 cfu/m3) and SS4 (15 cfu/m3) at ambient sampling and 40 cfu/m3, 41.6 cfu/m3, 58.3 cfu/m3, 68.3 cfu/m3, 6.6 cfu/m3, 31.6 cfu/m3, 56.6 cfu/m3 and 25 cfu/m3 respectively at populated sampling. Bacterial isolates identified were Lactobacillus, Staphylococcus, Bacillus, Rothia, Kurthia, Corynebacterium, Pseudomonas, Brevibacterium, and Flavobacterium. Statistical analysis of the results revealed negative relationships between class area and aerobic plate counts (p>0.05), class population and aerobic plate count (p>0.805), and significant increase in aerobic plate counts at populated conditions over that at ambient conditions (p<0.05). The results therefore point to the dimensions of classrooms, ventilation and population of the classrooms as important factors in determining the bacterial air quality, and invariably affecting the health condition of students.

scholarly journals Screening and characterization of Phosphorus solubilizing Bacteria and their effect on Rice seedlings

2015 ◽  
Vol 1 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Md Shahinur Rahman ◽  
Quazi Forhad Quadir ◽  
Atiqur Rahman ◽  
Moonmoon Nahar Asha ◽  
Md Abul Khair Chowdhury
Keyword(s):  
Soil Samples ◽  
Bacterial Isolates ◽  
Rice Seedlings ◽  
Gram Negative ◽  
P Content ◽  
Pure Cultures ◽  
Plant Trial ◽  
Solubilizing Capacity

An experiment was carried out to isolate, screen and characterize bacteria collected from an industrially polluted site of Bhaluka under the Mymensingh district and to evaluate their phosphorus (P) solubilizing capacity. About ten plant and soil samples from six different spots were collected from the site. Thirty four bacterial isolates were screened and pure cultures of the different bacterial isolates were prepared. Among the bacterial isolates 25 were gram negative and 9 were gram positive. About 31 bacterial isolates had catalase producing capacity and remaining 3 were negative to catalase test. Bacterial isolates were grown on a NBRIP media to determine their phosphorus solubilizing capacity. About 25 bacterial isolates were shown P solubilizing capacity. Isolate SB8 gave the highest result about 11.42 PSI (phosphorus solubilizing index), whereas other bacterial isolates showed moderate P solubilizing capacity (PSI 1.75-6.35). A plant trial with selected isolates (SB8, SB15, SB25) were also done and SB8 achieved 10% higher P content in comparison with control which supports the in vitro P solubilization assays. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22353 Res. Agric., Livest. Fish.1(1): 27-35, Dec 2014

The fine structure of conidium initiation in Ulocladium atrum

1974 ◽  
Vol 52 (3) ◽  
pp. 443-446 ◽  
Author(s):  
Fanny E. Carroll ◽  
George C. Carroll
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Agar Medium ◽  
Nutrient Agar ◽  
Vegetative Mycelium ◽  
Dialysis Membrane ◽  
Nutrient Agar Medium ◽  
Pure Cultures

Synchronous conidiation was induced in pure cultures of Ulocladium atrum by removal of a dialysis membrane overlay beneath which vegetative mycelium had been allowed to grow for 24 h on a solid nutrient agar medium. Samples taken [Formula: see text] and 6 h after the induction of sporulation contained numerous conidiophores with conidial initials in the very earliest stages of formation. Examination of sections of such conidiophores with the electron microscope revealed no suggestion of a discontinuity in the wall between the conidiophore and the conidial initial. We conclude that conidiation in Ulocladium atrum is holoblastic.

scholarly journals Valley Fever on the Rise—Searching for Microbial Antagonists to the Fungal Pathogen Coccidioides immitis

2019 ◽  
Vol 7 (2) ◽  
pp. 31 ◽  
Author(s):  
Antje Lauer ◽  
Joe Baal ◽  
Susan Mendes ◽  
Kayla Casimiro ◽  
Alyce Passaglia ◽  
...  
Keyword(s):  
Health Hazard ◽  
Bacterial Species ◽  
Bacterial Isolates ◽  
Coccidioides Immitis ◽  
Valley Fever ◽  
Streptomyces Spp ◽  
Pure Cultures ◽  
Arid Desert ◽  
Selection Of

The incidence of coccidioidomycosis, also known as Valley Fever, is increasing in the Southwestern United States and Mexico. Despite considerable efforts, a vaccine to protect humans from this disease is not forthcoming. The aim of this project was to isolate and phylogenetically compare bacterial species that could serve as biocontrol candidates to suppress the growth of Coccidioides immitis, the causative agent of coccidioidomycosis, in eroded soils or in areas close to human settlements that are being developed. Soil erosion in Coccidioides endemic areas is leading to substantial emissions of fugitive dust that can contain arthroconidia of the pathogen and thus it is becoming a health hazard. Natural microbial antagonists to C. immitis, that are adapted to arid desert soils could be used for biocontrol attempts to suppress the growth of the pathogen in situ to reduce the risk for humans and animals of contracting coccidioidomycosis. Bacteria were isolated from soil samples obtained near Bakersfield, California. Subsequently, pairwise challenge assays with bacterial pure cultures were initially performed against Uncinocarpus reesii, a non-pathogenic relative of C. immitis on media plates. Bacterial isolates that exhibited strongly antifungal properties were then re-challenged against C. immitis. Strongly anti-C. immitis bacterial isolates related to Bacillus subtilis and Streptomyces spp. were isolated, and their antifungal spectrum was investigated using a selection of environmental fungi.

scholarly journals IDENTIFICATION OF MICROBES ANTAGONISTIC AGAINST Fusarium oxysporum ISOLATED FROM RHIZOSPHERE ZONE OF WATERMELON

2018 ◽  
Vol 22 (2) ◽  
pp. 71
Author(s):  
Anak Agung Ngurah Nara-Kusuma ◽  
Yan Ramona ◽  
Meitini Wahyuni Proborini
Keyword(s):  
Fusarium Oxysporum ◽  
Agar Medium ◽  
Trichoderma Viride ◽  
Morphological Characteristics ◽  
Fungal Species ◽  
Reference Book ◽  
Vascular Wilt ◽  
Bacterial Isolates ◽  
Potato Dextrose Agar Medium ◽  
Nutrient Agar Medium

This research was aimed to isolate and identify microbes which antagonistic against Fusarium oxysporum, the causative agent of vascular wilt in watermelon plants. The antagonistic microbes were isolated from soil samples collected from rhizosphere of watermelon farm located at west Sanur village, South Denpasar, Bali.  Isolation of fungi and bacteria were conducted on potato dextrose agar medium (PDA) and nutrient agar medium (NA), respectively.  Fungal isolates were then observed under light microscope for its morphological characteristics before identification using a reference book.  Bacterial isolates were characterized using various tests, such as gram stain reaction, existence of endospores, catalase reaction, and ability to ferment various sugars.  Their characteristics were then compared with those described in a reference book.  Two fungal species (Trichoderma harzianum and Trichoderma viride) and two bacterial antagonists (Pseudomonas sp. and Bacillus sp.) were found to have potential to be developed as biocontrol agents to inhibit the growth of F. oxysporum. 

scholarly journals Potential impact of biocide adaptation on selection of antibiotic resistance in bacterial isolates

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Engy Elekhnawy ◽  
Fatma Sonbol ◽  
Ahmed Abdelaziz ◽  
Tarek Elbanna
Keyword(s):  
Antibiotic Resistance ◽  
Cross Resistance ◽  
Main Body ◽  
Bacterial Isolates ◽  
New Agents ◽  
Biocidal Products ◽  
Potential Impact ◽  
Emergence Of Resistance ◽  
Vital Factor ◽  
Selection Of

Abstract Background Antibiotic resistance in pathogenic bacterial isolates has increased worldwide leading to treatment failures. Main body Many concerns are being raised about the usage of biocidal products (including disinfectants, antiseptics, and preservatives) as a vital factor that contributes to the risk of development of antimicrobial resistance which has many environmental and economic impacts. Conclusion Consequently, it is important to recognize the different types of currently used biocides, their mechanisms of action, and their potential impact to develop cross-resistance and co-resistance to various antibiotics. The use of biocides in medical or industrial purposes should be monitored and regulated. In addition, new agents with biocidal activity should be investigated from new sources like phytochemicals in order to decrease the emergence of resistance among bacterial isolates.

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