scholarly journals Integrated Management Practices for Clubroot Disease (Plasmodiophora Brassicae Wor.) of Cauliflower in Palung, Makwanpur, Nepal

2020 ◽  
Vol 18 (1) ◽  
pp. 87-98
Author(s):  
P Adhikari ◽  
A Khatiwada ◽  
N Paneru ◽  
P Tandan
Keyword(s):  
Disease Control ◽  
Management Practices ◽  
Plasmodiophora Brassicae ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Block Design ◽  
Trichoderma Viride ◽  
Severity Index ◽  
Root Disease ◽  
Clubroot Disease

Club root is one of the most important diseases in the eastern hills of Nepal affecting the rural income as well as quantity and quality of Cauliflower production. A field experiment was conducted in Randomized Complete Block Design (RCBD) with 5 treatments and 4 replications during February to May, 2019. The experiment was conducted in the Farmer’s field in the disease prone area of Thaha municipality-4, Palung of Makwanpur district to assess the efficacy of five different treatments; Nebijin, Hatake, Trichoderma viride, Lime and control for the management or suppressing the club root disease. The effectiveness of the treatments against club root disease was evaluated along with their influence on growth parameters and yield parameters of white top variety of cauliflower. Different parameters such as disease incidence, percent disease index (PDI) or disease severity index (DSI) and percent disease control (PDI) were recorded using disease scoring scale. The treatments showed significant effect on the yield and disease parameters, but not on the vegetative parameters of cauliflower. The lowest disease incidence (50.2%) and severity index (26.8%) and the highest percent disease control (57.6%) was recorded in Nebijin. Moreover, the Marketable yield (Mtha-1) per plot was observed highest from Nebijin (48.27Mtha-1) and Trichoderma viride (47.39 Mtha-1) treatment. From the study it may concluded that the application of Nebijin was more effective for the management of clubroot disease of Cauliflower and the use of Hatake and Trichoderma virideas bio-fungicides were also giving the potential assuring measure for the controlling disease of Cauliflower. SAARC J. Agri., 18(1): 87-98 (2020)

scholarly journals Effect of Cultural and Biological Treatments in Managing Clubroot Disease of Cabbage in Sidhuwa, Nepal

2019 ◽  
Vol 7 (1) ◽  
pp. 96-101
Author(s):  
Ashish Ghimire ◽  
Sundarman Shrestha
Keyword(s):  
Disease Control ◽  
Disease Severity ◽  
Disease Incidence ◽  
Block Design ◽  
Combined Treatment ◽  
Severity Index ◽  
Disease Severity Index ◽  
Effective Microorganisms ◽  
Clubroot Disease ◽  
Biological Treatments

The Plasmodiophora brassicae Woronin is an important soil pathogen that attacks brassicaceae family of plants. The ability of pathogen to survive in soil for many years as a resting structure even in the absence of suitable host has increased its threat by making it more devastating. Lower effectiveness of treatments applied so far and failure to completely eradicate the disease once it enters the field has posed more problems. The study was conducted at a disease infected field in Sidhuwa, Dhankuta. It was focused on the effectiveness of different cultural and biological treatments against clubroot disease under field conditions. The treatments; lime, combination of lime and vermicompost, Effective Microorganisms (EM) solution and Trichoderma viride were arranged in Randomized Complete Block Design and replicated four times. The yield and yield attributing parameters and clubroot scale were recorded at the time of harvest. Values like Disease Incidence, Disease Severity Index and Disease Control Percentage were calculated based on clubroot scale value. The treatments showed no significant influence on vegetative parameters of the plants and on disease incidence. However, the combined treatment of lime and vermicompost was found to be most effective regarding disease severity and disease control i.e. the least Disease Severity Index (31.25%) and highest Disease Control Percentage (50.94%) compared to the control were obtained from the combined treatment of lime and vermicompost. Effective Microorganisms showed the highest disease severity. The combination of lime and vermicompost used as a treatment yielded highest marketable head weight (1.779 kg) and head diameter (19.90 cm). Int. J. Appl. Sci. Biotechnol. Vol 7(1): 96-101

scholarly journals Severe and Widespread Clubroot Epidemics in Nepal

Plant Disease ◽  
2008 ◽  
Vol 92 (2) ◽  
pp. 317-317 ◽  
Author(s):  
R. D. Timila ◽  
J. C. Correll ◽  
V. R. Duwadi
Keyword(s):  
Disease Severity ◽  
Management Practices ◽  
Plasmodiophora Brassicae ◽  
Sandy Loam ◽  
Trichoderma Viride ◽  
Economic Loss ◽  
Clubroot Disease ◽  
Brassica Vegetables ◽  
Ph Range ◽  
Production Areas

Cultivation of brassica vegetables has the highest potential for generating income among more traditional rice and maize farmers in Nepal. Among brassica vegetables, the most important are cauliflower (Brassica oleracea var. botrytis L.) and cabbage (B. oleracea var. capitata L.). Although clubroot disease, caused by Plasmodiophora brassicae Woronin, has been observed in Nepal since 1993, severe and widespread epidemics have been observed since 2004 in the Bhaktapur, Kathmandu, Lalitpur, and Palung Valley production areas. Typical disease symptoms (1) are widespread, and disease severity has been particularly severe in the Kathmandu Valley and Palung/Daman area of the Makwanpur District. Many cauliflower fields in these areas have had as much as 100% yield loss between 2004 and 2006 with an estimated 40% overall loss from clubroot. Estimates from interviews with growers in the Palung production area during an intensive farmers' interaction program indicated that cauliflower production was reduced from 5 to 6 metric tons per household (1,500 m2) prior to 2004 to <300 kg per household in 2004 and beyond. The economic loss in this area alone was estimated at $1.4 million in 2004 and 2005. Examination of transplant nurseries indicated that frequently >80% of the seedlings have symptoms of clubroot at the time of transplanting. Soil samples from throughout the production areas indicated that the sandy loam soils were predominately acidic (pH range of 4.2 to 7.2 with >90% below 6.0). Several management practices are being employed to reduce disease severity, including the use of clubroot resistant cultivars, raising the soil pH to >7.0 by using dolomitic lime, testing of the fungicide flusulfamide (Nebijin) and biopesticide Sanjeevani (Trichoderma viride), and biofumigation and solarization of the nursery beds in an effort to reduce disease pressure on transplant material. References: (1) G. R. Dixon. Compendium of Brassica Diseases. S. R. Rimmer et al., eds. American Phytopathological Society, St. Paul, 2007.

scholarly journals First Report of Clubroot on Capsella bursa-pastoris Caused by Plasmodiophora brassicae in Sichuan Province of China

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 687-687 ◽  
Author(s):  
L. Ren ◽  
X. P. Fang ◽  
C. C. Sun ◽  
K. R. Chen ◽  
F. Liu ◽  
...  
Keyword(s):  
Plasmodiophora Brassicae ◽  
Tap Water ◽  
Disease Incidence ◽  
Morphological Characteristics ◽  
Pcr Amplification ◽  
Sichuan Province ◽  
Clubroot Disease ◽  
Cruciferous Vegetable ◽  
First Report ◽  
Resting Spores

Shepherd's purse (Capsella bursa-pastoris (L.) Medicus) is an edible and wild medicinal plant widely distributed in China. This plant has been cultivated in Shanghai, China, since the end of the 19th century. Infection of C. bursa-pastoris by Plasmodiophora brassicae, the causal agent of clubroot disease on Brassica spp. has been reported in Korea (2), but is not known to occur in China. In February of 2011, stunted and wilted shepherd's purse (SP) plants were observed in a field planted to oilseed rapes (B. napus) in Sichuan Province of China. Symptomatic SP plants also exhibited root galls. Disease incidence was 6.2% and 100% for SP and B. napus, respectively. Root galls on diseased SP plants were collected for pathogen identification. Many resting spores were observed when the root galls were examined under a light microscope. The resting spores were circular in shape, measuring 2.0 to 3.1 μm in diameter (average 2.6 μm). PCR amplification was conducted to confirm the pathogen. DNA was extracted from root galls and healthy roots (control) of SP. Two primers, TC2F (5′-AAACAACGAGTCAGCTTGAATGCTAGTGTG-3′) and TC2R (5′-CTTTAGTTGTGTTTCGGCTAGGATGGTTCG-3′) were used to detect P. brassicae (1). No PCR amplifications were observed with the control DNA as template. A fragment of the expected size (approximately 520 bp) was obtained when DNA was amplified from diseased roots of SP. These results suggest that the pathogen in the galled roots of SP is P. brassicae. Pathogenicity of P. brassicae in SP was tested on plants of both SP and Chinese cabbage (CC) (B. campestris ssp. pekinensis). A resting spore suspension prepared from naturally infected SP roots was mixed with a sterilized soil in two plastic pots, resulting in a final concentration of 5 × 106 spores/g soil. Soil treated with the same volume of sterile water was used as a control. Seeds of SP and CC were pre-germinated on moist filter paper for 2 days (20°C) and seeded into the infested and control pots, one seed per pot for planted for CC and four seeds per pot for SP. The pots were placed in a chamber at 15 to 25°C under 12 h light and 12 h dark. Plants in each pot were uprooted after 4 weeks and the roots of each plant were washed under tap water and rated for clubroot disease. No disease symptoms were observed in the control treatments of SP or CC. Plants of both species showed symptoms of clubroot, with the disease incidence of 62.5% and 100% on SP and CC, respectively. The pathogen was isolated from diseased roots of each plant and confirmed as P. brassicae based on morphological characteristics and PCR detection. To our knowledge, this is the first report of clubroot disease on C. bursa-pastoris in Sichuan Province of China. This finding suggests that it may be necessary to manage C. bursa-pastoris in cruciferous vegetable (cabbage, turnip) and oilseed rape production fields. References: (1) T. Cao et al. Plant Dis. 91:80, 2007. (2) W. G. Kim et al. Microbiology 39:233, 2011.

Eco-friendly management of wilt caused by fusarium oxysporum f.sp. Ciceri in chickpea

2015 ◽  
Author(s):  
Chandar Kala ◽  
S. Gangopadhyay ◽  
S. L. Godara
Keyword(s):  
Population Dynamics ◽  
Disease Control ◽  
Fusarium Oxysporum ◽  
Seed Treatment ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Organic Amendments ◽  
Chickpea Wilt ◽  
Maximum Inhibition

Antagonistic potentiality of Trichoderma viride, T. harzianum and Pseudomonas fluorescens were evaluated against Fusarium oxysporum f. sp. ciceri under in vivo conditions. The effect of organic amendments viz; farm yard manure, vermicompost and mustard cake on disease control potentiality of test antagonists against chickpea wilt and on population dynamics of the antagonists and pathogen in soil was also studied. Maximum inhibition of mycelial growth of F. o. f. sp. ciceri was recorded in presence of P. fluorescens (%) followed by T. harzianum (%) and T. viride (%). Seed treatment with P. fluorescens was more effective in suppressing the disease incidence as compared to T. harzianum and T. viride. The disease control efficacy and population dynamics of all the three test antagonists was enhanced in response to application of organic amendments. Among the three organic amendments tested, mustard cake was most effective in enhancing the disease control potentiality of these antagonists.

scholarly journals Efficacy of Different Fungicides Against Rhizome Rot Disease of Ginger Under Farmer Field Condition in Salyan, Nepal

2021 ◽  
Vol 6 (6) ◽  
Author(s):  
Resham B ◽  
◽  
Amrita P ◽  
Anish P ◽  
Bikash G ◽  
...  
Keyword(s):  
Field Experiment ◽  
Disease Control ◽  
Disease Severity ◽  
Disease Incidence ◽  
Trichoderma Viride ◽  
Germination Percentage ◽  
Tiller Number ◽  
Leaf Number ◽  
Rot Disease ◽  
Rhizome Rot

A study was conducted from March to August 2020 to evaluate the effectiveness of different fungicides for the management of rhizome rot disease in ginger. The field experiment was conducted under Randomized Complete Block Design with four replication and five treatments; Carbendazim 50% WP @ 1gm/litre + Mancozeb 75% WP @ 2.5gm/litre, Metalaxyl 8% WP + Mancozeb 64% WP @ 2gm/litre, Carbendazim 50% WP @ 1gm/litre, Trichoderma viride @ 5gm/litre and control. The height of pseudo stems, leaf number, germination percentage, tiller number, disease incidence, disease severity and percentage of disease control was recorded during field experiment. There was no significant effect of pre sowing rhizome treatment in height of pseudostems, leaf number and germination percentage. The highest tiller number (3.40) was found with Carbendazim 50% WP @1gm/litre + Mancozeb 75% @ 2.5gm/ litre treatment and lowest tillers number (2.95) was found with control. The highest disease incidence (34.37) and severity (30.03) was found in control plot whereas the lowest disease incidence (2.58) and disease severity (2.46) was found with Carbendazim 50% @ 1gm/litre treatment + Mancozeb 75% @ 2.5gm/litre. Similarly, the highest percentage disease control (85.61) was found with Carbendazim 50% @ 1gm/litre + Mancozeb 75% @ 2.5gm/litre treated rhizome and the lowest (54.14) was found with Trichoderma viride @ 5gm/litre treatment. Therefore, seed treatment with Carbendazim 50%WP @ 1gm/litre + Mancozeb 75% WP @ 2.5 gm/litre is found effective among other fungicides in Bagchaur-3, Salyan and is recommended for enhancing tiller and reducing rhizome rot disease incidence and severity in ginger.

scholarly journals Eco-friendly management of major diseases in jute (Corchorus olitorius L.)

2014 ◽  
Vol 6 (2) ◽  
pp. 541-544 ◽  
Author(s):  
P. N. Meena ◽  
A. Roy ◽  
B. S. Gotyal ◽  
S. Mitra ◽  
S. Satpathy
Keyword(s):  
Seed Treatment ◽  
Disease Incidence ◽  
Block Design ◽  
Trichoderma Viride ◽  
Corchorus Olitorius ◽  
Farm Yard Manure ◽  
Soil Application ◽  
Randomized Block Design ◽  
Fibre Yield ◽  
Cent Disease

Field experiment was conducted for disease management with seven eco-friendly treatments in Corchorus olitorius jute during 2012-2013 under randomized block design (RBD) in three replications with variety JRO-524. The ecofriendly treatment 50% N: P: K + seed treatments with Azotobacter and phosphorus solubilizing bacteria (PSB) @ 5g/Kg+ Trichoderma viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + Psuedomonas fluorescens spray @ 0.2% at 45DAS was found superior with minimum per cent disease incidence (3.46 and 1.43%) as compared to control (13.17 and 4.96%). The second best treatment was found with Farm yard manure (FYM) @ 5t/ha + seed treatment with Azotobecter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha 21DAS)+ P. fluorescencs spray @ 0.2% at 45DAS and showed 3.61 and 2.0% disease incidence. The treatment with N: P: K @ 60:30:30 and seed treatment with carbendazim 50WP @ 2g/Kg at 45DAS showed 4.56 and 2.10 % disease incidence. Dry fibre yield was highest (30.33q/ha) in the plots treated with FYM @ 5t/ha + seed treatment with Azotobacter and PSB @ 5g/Kg+ T. viride (seed treatment @ 5g/Kg of seed and soil application @ 2Kg/ha at 21DAS) + P. fluorescencs spray @ 0.2% at 45DAS) and it was lowest in untreated control (15.69 q/ha).Variation in yield was attributed due to difference in plant height.

scholarly journals Short Communication: The potential of various indigenous Trichoderma spp. to suppress Plasmodiophora brassicae the pathogen of clubroot disease on cabbage

2017 ◽  
Vol 18 (4) ◽  
pp. 1424-1429
Author(s):  
I KETUT SUADA
Keyword(s):  
Short Communication ◽  
Plasmodiophora Brassicae ◽  
Disease Incidence ◽  
Economic Value ◽  
Attractive Alternative ◽  
Trichoderma Spp ◽  
Clubroot Disease ◽  
Local Soil ◽  
Soil Media ◽  
Trichoderma Isolates

Suada IK. 2017. Short Communication: The potential of various indigenous Trichoderma spp. to suppress Plasmodiophora brassicae, the pathogen of clubroot disease on cabbage. Biodiversitas 18: 1424-1429. On the island of Bali, cabbage (Brassica oleracea L.) is a vegetable that has high economic value especially for farmers who cultivate it as a cash crop. However, the clubroot disease caused by the microbial pathogen Plasmodiophora brassicae decreases its productivity. Much effort has been expended in attempts to decrease the pathogen attack by use of synthetic fungicides, however, this has not had much success; more over such pesticide applications generate environmental pollution. Therefore, biological control using Trichoderma or other organisms antagonistic to the pathogen is an attractive alternative. The purpose of this study was to find, isolate and identify Trichoderma spp. able to decrease the disease incidence and increase plant growth. Twelve indigenous Trichoderma isolates were tested on cabbage plants grown in polybags containing local soil media culture treated with the Trichoderma inocula at a concentration of 1.5x106 CFU per polybag. The results showed that three Trichoderma isolates, i.e. T. hamatum-1, T. harzianum-1, and T. harzianum-2, were able to decrease the clubroot disease and increase cabbage growth as well.

scholarly journals EFFECTIVENESS OF THE APPLICATION OF ORGANIC MATTER AND TRICHODERMA VIRIDE FROM SUPPRESIVE SOIL TO CONTROL FUSARIUM WILT ON BANANA PLANT

2019 ◽  
Vol 18 (2) ◽  
pp. 119
Author(s):  
Ivayani Ivayani ◽  
Cipta Ginting ◽  
Yusnita Yusnita ◽  
Suskandini Ratih Dirmawati
Keyword(s):  
Organic Matter ◽  
Fusarium Wilt ◽  
Disease Incidence ◽  
Block Design ◽  
Trichoderma Viride ◽  
Experimental Unit ◽  
Banana Plant ◽  
Design Data ◽  
Soil Medium ◽  
Banana Production

Effectiveness of the application of organic matter and Trichoderma viride from suppresive soil to control fusarium wilt on banana plant. Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense is one of the problems in banana production. This research was aimed to evaluate the effect of  Trichoderma viride isolated from suppresive soil and organic matter on controlling fusarium wilt on banana plant. The efficcacy trial consisted of 12 treatments and four replications within each treatment. Each experimental unit consisted of 10 plants. Organic matters used were rice straw, cassava peel, and compost. Treatments were arranged in a randomized complete block design. Data obtained were analyzed by analysis of variance followed by orthogonal analysis (P≤0.05). Application of T. viride suppressed disease incident up to 65%. The highest disease incident occurred in plants treated without T. viride and organic matter and in plants treated with organic matter only (78.33%). Disease incidence in plants treated with T.viride was lower than those treated with the combination of T. viride and organic matter. Different application times (in nursery or soil medium) did not significantly affect fusarium wilt incidence. The type of organic matter did not significantly affect the incidence and severity of fusarium wilt.

scholarly journals APPLICATION OF Trichoderma spp. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (Plasmodiophora brassicae WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE

2019 ◽  
Vol 6 (2) ◽  
pp. 79
Author(s):  
I Ketut Suada ◽  
Anak Agung Ngurah Gede Suwastika ◽  
I Kadek Ngestika Pradnyana ◽  
Nataliya Shchegolkova ◽  
Rodion Poloskin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plasmodiophora Brassicae ◽  
Disease Incidence ◽  
Block Design ◽  
Economic Value ◽  
Dry Weight ◽  
Trichoderma Spp ◽  
Population Number ◽  
Bacteria Growth ◽  
Root Dry Weight

The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%.  This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.

scholarly journals Evaluating the Performance of Different Management Practices for Control of Leaf Curl Disease of Chili in Kharif Season of Chatra District in Jharkhand

2019 ◽  
pp. 1-5
Author(s):  
Ranjay Kumar Singh ◽  
Dharma Oraon ◽  
Upendra Kumar Singh ◽  
Zunaid Alam
Keyword(s):  
Management Practices ◽  
Disease Incidence ◽  
Block Design ◽  
Net Income ◽  
Soil Application ◽  
Kharif Season ◽  
Gross Income ◽  
Leaf Curl Disease ◽  
Gm Plant ◽  
Leaf Curl

An On-farm trial (OFT) was conducted for evaluating the performance of different management practices for control of leaf curl disease of chilli in Kharif season in Chatra district of Jharkhand in two consecutive Kharif seasons of the year 2016-17 and 2017-18. The experiment was conducted in Amgawa village of Simariya block in Chatra district, where farmers generally grow Chili as a cash crop in medium land. Trial was designed in randomized block design consisting of 25 replication with three technological options i.e. TO-I:  Soil application of carbofuran granule @2 gm/plant before transplanting, TO-II – TO-I: Soil application of carbofuradon granule @2 gm/plant before transplanting, + Spray of Spinosad 48 EC @ 2 ml/lit of water after disease incidence twice after 15 days interval and TO-III – TO-I: Soil application of carbofuradon granule @2 gm/plant before transplanting, +Spray of Acephate 75SP @ 2 ml/lit of water after disease incidence twice after 15 days interval besides farmers practices. For evaluation of the performance, percentage infestation of leaf curl disease on plant/5 sqm and yield q/ha was considered, as for economic analysis gross income, net income and B.C. ratio was calculated and compared with all treatments.  The result indicated that the minimum thrips population was found in technological option TO-III (3.35) followed by TO-II (6.45) and TO-I (6.40) respectively with a higher yield (84.5q/ha). Gross income, Net income and BC ratio was also found more in technological option TO-III. So that technology option TO-III i.e. –soil application of carbofuradone granule @ 2gram/plant before transplanting spray of acephate 75SP @ 2ml/lit of water after disease incidence twice after 15 days interval recommended considering  Bio-physical and socio-economic condition of Chatra district for control of leaf curl disease for resource-rich and resource-poor farmers. This recommendation was also given to ATMA and N.G.Os of the district for faster dissemination among the farming community.

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