scholarly journals Comparative study on the microbiological quality of vegetables collected from local markets and super shops of Dhaka city

2021 ◽  
Vol 11 (1) ◽  
pp. 24-27
Author(s):  
Mofijur Rahman Mamum ◽  
Kamal Kanta Das ◽  
Md Sohel Rana ◽  
Halimatus Sadia Tanim ◽  
Farahnaaz Feroz
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Local Market ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Vegetable Samples ◽  
Lady’S Finger ◽  
Bacteria And Fungi

Vegetables are major source of vitamins and fibers but presence of pathogens in salad vegetables can cause outbreak of diseases. Several studies have been conducted on commonly consumed vegetables so far to detect the level of microbial contamination. However, few of them compare the microbial quality of local and super shop vegetables. The present study was conducted for microbiological assessment and comparing four types of vegetables collected from two different market conditions. In this study, Green chili (Capsicum frutescens L.), Tomato (Solanum lycopersicum), Coriander (Coriandrum sativum) and Lady’s finger (Abelmoschus esculentus) were analyzed to determine Total Viable Bacterial (TVB) Counts, Total Fungal (TF) Counts, Total Coliform Counts (TCC), Total Fecal Coliform (TFC) Counts and occurrence of Staphylococcus aureus, Pseudomonas spp., Listeria spp., Bacillus spp., Salmonella spp., and Vibrio spp. Among these microorganisms, Klebsiella spp. and Pseudomonas spp. were significantly present in coriander of both local market and super shop. All pathogenic bacteria and fungi were found in huge amounts in the vegetable samples of the local market. Although some pathogenic bacteria and fungi were also found in the same samples of the super shop, relatively lower than the local market’s vegetables. In conclusion, contamination in collected vegetable samples were not acceptable but comparatively higher load in local market samples indicated that the hygienic condition in these markets is not sufficiently maintained. Stamford Journal of Microbiology, Vol.11 (1) 2021: 24-27

scholarly journals Incidence of antibiotic resistant pathogenic bacteria in vegetable items sold by local and super shops in Dhaka city

2015 ◽  
Vol 4 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Ahmed Kabir ◽  
Ashish Kumar Das ◽  
Md Shahidul Kabir
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Local Market ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Vegetable Samples

The present study was carried out to investigate the microbiological quality and antibiotic resistance patterns of pathogenic bacteria isolated from vegetable samples. A total of 14 vegetable samples, 7 from local and 7 from super shops, were randomly collected from different locations of Dhaka city. Concentrations of total heterotrophic bacteria, total coliform, faecal coliform, Pseudomonas spp., Listeria spp. and Staphylococcus aureus were enumerated from each sample by serial dilution and spread plate technique. Presence of Salmonella spp., Shigella spp. and Vibrio spp. were determined by enrichment and selective plating methods. Antibiotic sensitivity patterns of the isolated bacteria were determined using Imipenem (10 ?g), Ceftriaxone (30 ?g), Sulphamethoxazole (25 ?g), Ampicillin (10 ?g), Gentamicin (10 ?g), Aztreonam (30 ?g), Cefuroxime (30 ?g) and Oxacillin (5 ?g) antibiotic discs. The local market vegetables showed higher proportions of E. coli (4/7, 57.14%) but the super shop vegetables showed higher proportions of Pseudomonas spp. (5/7, 71.42%) and Listeria spp. (5/7, 71.42%). Pathogenic bacteria isolated form the super shops showed increased resistance against (5/8, 62.5%) antibiotics tested against the pathogenic bacteria. Contamination of vegetables by a range of pathogenic bacteria in local and super market vegetables is a serious threat to public health if they are consumed raw or unprocessed. Higher antibiotic resistance in pathogens isolated form supermarket vegetables needs to be investigated in order to monitor and control spread of infections with drug resistant bacteria. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22755 Stamford Journal of Microbiology, Vol.4(1) 2014: 13-18

scholarly journals Microbiological analysis of pasteurized and fresh fruit juice sold in Rawalpindi of Pakistan

2013 ◽  
Vol 48 (3) ◽  
pp. 185-192 ◽  
Author(s):  
SA Batool ◽  
SS Tahir ◽  
N Rauf ◽  
R Kalsoom
Keyword(s):  
Fruit Juice ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Fruit Juices ◽  
Local Market ◽  
Microbiological Analysis ◽  
E Coli ◽  
Bacteria And Fungi

Freshly prepared and pasteurized fruit juices sold by vendors in local market of Rawalpindi city from 10 locations were analyzed for the microbiological quality. Total viable count (TVC), total coliform, faecal coliform, molds and the presence of pathogenic microorganisms such as E. coli, Pseudomonas, Staphylococcus aureus, Salmonella, and fungi like Aspergillus, Pencillum, Rhizopus were determined .In open fruit juices available in city were highly contaminated with bacteria and fungi. E. coli, Salmonella, Staphylococcus, and Pseudomonas were isolated with different frequency. Aspergillus, Pencillum and Rhizopus were also found in juices especially Aspergillus was with high percentages. The pasteurized juices have less contamination as compared to the fresh juices samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17329 Bangladesh J. Sci. Ind. Res. 48(3), 185-192, 2013

Assessment of the Microbiological Quality of Ready-To-Use Vegetables for Health-Care Food Services

1997 ◽  
Vol 60 (8) ◽  
pp. 954-960 ◽  
Author(s):  
JOSEPH A. ODUMERU ◽  
SHEILA J. MITCHELL ◽  
DAVID M. ALVES ◽  
JOHN A. LYNCH ◽  
ARLENE J. YEE ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Food Services ◽  
Salmonella Spp ◽  
E Coli ◽  
Vegetable Samples ◽  
Serotype O ◽  
Select Group ◽  
Before And After

The microbiological quality of ready-to-use (RTU) vegetables, including chopped lettuce, salad mix, carrot sticks, cauliflower florets, sliced celery, coleslaw mix, broccoli florets, and sliced green peppers was determined before and after processing. Microbial profiles were obtained 24 h after processing and on days 4, 7, and 11 after storage at 4 and 10°C to simulate temperature abuse. In addition, the microbial profiles of four RTU vegetables, coleslaw mix, salad mix, cauliflower florets, and sliced green peppers were determined 7 days after distribution to a select group of Ontario hospitals. RTU vegetables, with the exception of green peppers, showed up to a 1-log decrease in aerobic colony counts after processing. These counts increased to preprocessing levels after 4 days of storage at both 4 and 10°C. RTU vegetables stored at temperature abuse conditions (10°C)had significantly higher counts (P < 0.001) on days 4 to 11 as compared to those stored at 4°C. Green peppers had the highest bacterial counts while cauliflower and chopped lettuce had the lowest counts at both storage temperatures (P < 0.05). Increased levels of Listeria monocytogenes in RTU vegetables were associated with temperature abuse. Levels of >100 MPN/g for L. monocytogenes were detected in 8 of 120 (6.7%) samples stored at 10°C but not in 175 samples stored at 4°C after 7 days (P < 0.05). Overall, L. monocytogenes was detected in 13 of 120 (10.8%) RTU vegetables stored for up to 11 days at 10°C and 5 of 176 (2.8%) samples stored at 4°C (P < 0.05). E. coli was detected in 2 of the 120 (1.7%) processed RTU vegetables after day 7 of storage at 10°C and 1 of the 65 (1.5%) unprocessed vegetables from the same batches of vegetables used for processing. This indicator organism was not detected in RTU vegetable samples stored at 4°C or in any of the RTU vegetable samples obtained from hospital coolers. Other pathogenic bacteria, such as Salmonella spp., Campylobacter spp., Yersinia enterocolitica (serotype O:3) and verocytotoxigenic E. coli (VTEC) were not detected in any of the RTU vegetables tested, Recommendations regarding processing, distribution, and storage of these products are presented.

scholarly journals Tokat Bez Sucuğunun Mikrobiyolojik Kalitesinin İncelenmesi

2020 ◽  
Vol 8 (12) ◽  
pp. 2683-2694
Author(s):  
Nesrin Kaval ◽  
Nilgün Öncül ◽  
Zeliha Yıldırım
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Aerobic Bacteria ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Microbiological Parameters

In this study, the microbiological quality of traditionally produced Bez Sucuk in Tokat and its vicinity was investigated. For this purpose, 30 Bez Sucuk samples obtained from butchers, producers of Bez Sucuk, were analyzed for total count of mesophilic aerobic bacteria, lactic acid bacteria, yeasts-moulds, total coliform, fecal coliform, Staphylococcus aureus, Bacillus cereus, and Cl. perfringens. Also, the presence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. were investigated in all samples. pH and water activity of the samples were determined. As the result of the analysis, the counts of the microbiological parameters investigated were found as follows: total mesophilic aerobic bacteria 3.5×106-4.23×109 CFU/g, lactic acid bacteria 5.55×105-2.45×109 CFU/g, yeasts and moulds 2.50×103-6.90×109 CFU/g, total coliform

Microflora of smoked Trachurus trachurus and Scomber scombrus samples in Orlu South East, Nigeria and its implication on public health

2020 ◽  
Vol 34 (1) ◽  
pp. 7-14
Author(s):  
L.A. Agbabiaka ◽  
C.O. Agu
Keyword(s):  
Public Health ◽  
Microbiological Quality ◽  
Viable Count ◽  
Trachurus Trachurus ◽  
Salmonella Spp ◽  
Candida Spp ◽  
Fish Samples ◽  
Bacteria And Fungi ◽  
And Staphylococcus Aureus

The study was carried out to assess the microbiological quality of smoked Trachurus trachurus and Scomber scumbrus in four markets in Orlu Local Government Area, South East, Nigeria. Forty freshly smoked samples of Trachurus trachurus and Scomber scumbrus were procured from Eke Eziachi, Orlu Main, Aforaja Umuowa and Afor Ogidi Ihioma markets and analyzed using standard techniques. Results revealed that Orlu main market had the highest level of microbial contamination (mean bacteria and fungi count of 5 x 102 and 10 x 102cfu/g respectively). The highest total viable count (7 x102 cfu/g) of Scomber scumbrus was obtained in Aforaja Umuowa market while the least value of 2 x102 cfu/g was observed in Afor Ogidi Ihioma market. Also, similar value was recorded in Trachurus trachurus at Aforaja Umuowa market. Results from the study indicated that pathogens present in fish samples included bacteria (Bacillus cereus, B. subtilis, Escherichia coli, Salmonella spp and Staphylococcus aureus) and fungi (Gliocladium spp., Candida spp., Rhizopus spp., Alternaria spp., Aspergillus spp., Clasdosprium spp., Fonsecaca spp., Chrysoporius spp., mucour spp. and Botrytis spp). The study also showed that freshly smoked Trachurus trachurus and Scomber scumbrus samples sold in the four markets under assessment were contaminated with pathogenic organisms. This may however present some public health implications. Keywords: Microbial activity, Smoked fish, Orlu, Fish quality; Hygiene

scholarly journals Detection of drug resistant bacteria in different ice creams available in local market of Dhaka city

2017 ◽  
Vol 7 (1) ◽  
pp. 33-35
Author(s):  
Md Musa Howlader ◽  
Tahmina Shammi
Keyword(s):  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Ice Cream ◽  
Storage Condition ◽  
Resistant Bacteria ◽  
Local Market ◽  
Drug Resistant ◽  
Drug Resistant Bacteria ◽  
Bacteria And Fungi

Sound microbiological quality of ice cream should be ensured for being a widely popular dairy food in the world. Present study was conducted to determine the microbiological quality of different ice cream samples available in Dhaka, Bangladesh. Total 3 ice cream samples were collected and processed to detect the microbiological quality as well as drug resistant trait of the isolates through several conventional Kirby Bauer method. All the samples were found to be contaminated with the total viable bacteria and fungi within the range of 1.2×104 cfu/ml to 4.3×106 cfu/ml. The presence of E. coli, Staphylococcus aureus was also observed up to 104 cfu/ml. However, the fecal contamination was totally absent in all the samples. Antibiotic profile of two isolates was measured against 8 commonly used antibiotics and both E.coli and Staphylococcus aureus were found to be resistant against more than one antibiotics. Appropriate hygienic and storage condition should be maintained concerning the health safety of consumers. Stamford Journal of Microbiology, Vol.7(1) 2017: 33-35

scholarly journals Contributions to the Knowledge of Microbiological Quality of Pasteurized Melange Produced in Romania

2016 ◽  
Vol 73 (2) ◽  
pp. 139 ◽  
Author(s):  
Andreea Catalina NISTOR ◽  
Lucia Iuliana NISTOR ◽  
Claudia Gabriela DUMINICA ◽  
Marius Giorgi USTUROI
Keyword(s):  
Heat Treatment ◽  
Bacterial Contamination ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Egg Products ◽  
Bacillus Spp ◽  
Microbiological Indicators ◽  
Production Conditions

Today's consumers show an affinity for quality products produced under production conditions that strictly observes the principles of food safety. Bacterial contamination of eggs and egg products can thus be a source of disease for the human consumer with the necessary effects on the sector of activity.The term "egg products" defines the components of the egg (separate or as a mixture-melange) released from their shell and submitted to a heat treatment, they are forming foods rich in protein, vitamins and trace elements of high quality . The purpose of this study was to establishing and monitoring the microbiological indicators of melange produced in our country. The study was carried out on the pasteurized egg mix, packaged "bag-in-box" (5 units package), and stored for a period of 25 days at the temperature recommended by the manufacturer (0 ... + 4 °C). The actual analysis is to determine NTGMA, Enterobacter spp., Bacillus spp., Pseudomonas spp., and Salmonella spp. Regarding developments of NTGMA in pasteurized melange stored for 25 days, our data showed an increase of 75.22% compared to the situation found on fresh product (3.656 log cfu/ml vs 6.406 log cfu/ml). The situation was similar for Enterobacter spp. (119.64% increase), Bacillus spp. (76.27% increase) and Pseudomonas spp. (58.72% increase). Salmonella spp. was not identified in the product tested by us. The heat treatment of pasteurized liquid melange does not destroy completely the initial microbial load, therefore certain precautions are needed to store these foods.

scholarly journals Microbiological quality of surimi-based imitation crab

2018 ◽  
Vol 51 (2) ◽  
pp. 134
Author(s):  
N. SOULTOS (Ν. ΣΟΥΛΤΟΣ) ◽  
A. ABRAHIM (Α. ΑΜΠΡΑΧΙΜ) ◽  
E. THEOLOGIDOU (Ε. ΘΕΟΛΟΓΙΔΟΥ) ◽  
Pr. KARAIOANNOGLOU (ΠΡ. ΚΑΡΑΪΩΑΝΝΟΓΛΟΥ) ◽  
A. KANSOUZIDOU (Α. ΚΑΝΣΟΥΖΙΔΟΥ)
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Storage Period ◽  
Coliform Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Microbial Indices

A survey was conducted to evaluate the microbiological quality of sliced, vacuumpacked, surimi-based imitation crab at the retail level, during a 3-month storage period. The roll shaped product imported from France, was sliced and packed in a fishery processing plant in the region of Thessaloniki. Twenty five samples were examined each month, and a total of 75 samples were examined during the three month storage period of the product. Psychrotropic, lactic acid and coliform bacteria, Escherichia coli I, yeasts, Staphylococcus aureus, Salmonella spp., Listeria spp. and Escherichia coli Θ157:Η7, were used as microbial indices, to evaluate the microbiological quality of the product. The following conclusions were derived from the evaluation of the results of the microbiological examinations. 1) The microbiological condition of the crab meat analogs examined on the 15th day of storage, was found satisfactory, since microbial populations were very low, while pathogenic bacteria were not detected in any of the examined samples. 2) The overall microbiological quality of the samples examined at the end of the second month of storage, was also good, since psychrotrophic and lactic acid bacteria exceeded 1Θ7 log cfu/g only in one sample), while coliform bacteria were not detected in 60% of the samples and Escherichia coli I was detected only in one sample. The mean value of the yeasts was 2.84 log cfu/g, while molds were not detected in any of samples examined. 3) The microbiological quality of the samples examined at the end of the three months storage period was not satisfactory, since the microbial indices in the majority of the samples (80%), were rather high. S. aureus, Salmonella spp, Listeria s pp. and E. coli 0157:H7 were not detected in any sample, during the three month storage period.

scholarly journals Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic

2020 ◽  
Author(s):  
Gwladys S. Komagbe ◽  
Philippe Sessou ◽  
François Dossa ◽  
Prudencio Sossa-Minou ◽  
Bernard Taminiau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Compliance Rate ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Salmonella Spp ◽  
Potential Health ◽  
Antibiotic Susceptibility Test ◽  
Aerobic Flora

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.

Microbiological quality of a waste stabilization pond effluent used for restricted irrigation in Valle Del Cauca, Colombia

2002 ◽  
Vol 45 (1) ◽  
pp. 139-143 ◽  
Author(s):  
C.A. Madera ◽  
M.R. Peña ◽  
D.D. Mara
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Helminth Eggs ◽  
Waste Stabilization ◽  
Shigella Spp ◽  
Field Workers ◽  
Valle Del Cauca ◽  
Final Effluent

This paper discusses the applicability of effluent reuse in agriculture after treatment in a series of anaerobic, facultative and maturation ponds. The WSP system is located in Ginebra municipality, a small town in southwest Colombia. The total HRT is 12 days. Several samples of the final effluent were taken over a 55 day period and were analysed for E.coli, Streptococcus spp. and helminth eggs. Some additional grab samples were taken to determine the presence of pathogenic bacteria such as Salmonella spp. and Shigella spp. The results showed that the system was able to remove 4 log units of E.coli, 1 log unit of Streptococcus spp. and 100% of helminth eggs. Meanwhile, Salmonella spp. were detected in the effluent of the facultative pond whilst Shigella spp. were not detected in any sample. The main species of helminth eggs encountered were Taenia spp., Ascaris spp., Trichuris spp., Hymenolepis nana, H. diminuta and Enterobius vermicularis. Removal efficiencies were satisfactory despite the relatively short HRT. Nevertheless, WHO guidelines were slightly surpassed in the case of E.coli for unrestricted irrigation. The helminth egg value was always below the maximum WHO limit. Hence, this effluent can be safely used for restricted irrigation provided that field workers are protected from direct contact with wastewater given the presence of Salmonella spp. in the facultative pond effluent.

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