scholarly journals Incidence of antibiotic resistant pathogenic bacteria in vegetable items sold by local and super shops in Dhaka city

2015 ◽  
Vol 4 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Ahmed Kabir ◽  
Ashish Kumar Das ◽  
Md Shahidul Kabir
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Local Market ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Vegetable Samples

The present study was carried out to investigate the microbiological quality and antibiotic resistance patterns of pathogenic bacteria isolated from vegetable samples. A total of 14 vegetable samples, 7 from local and 7 from super shops, were randomly collected from different locations of Dhaka city. Concentrations of total heterotrophic bacteria, total coliform, faecal coliform, Pseudomonas spp., Listeria spp. and Staphylococcus aureus were enumerated from each sample by serial dilution and spread plate technique. Presence of Salmonella spp., Shigella spp. and Vibrio spp. were determined by enrichment and selective plating methods. Antibiotic sensitivity patterns of the isolated bacteria were determined using Imipenem (10 ?g), Ceftriaxone (30 ?g), Sulphamethoxazole (25 ?g), Ampicillin (10 ?g), Gentamicin (10 ?g), Aztreonam (30 ?g), Cefuroxime (30 ?g) and Oxacillin (5 ?g) antibiotic discs. The local market vegetables showed higher proportions of E. coli (4/7, 57.14%) but the super shop vegetables showed higher proportions of Pseudomonas spp. (5/7, 71.42%) and Listeria spp. (5/7, 71.42%). Pathogenic bacteria isolated form the super shops showed increased resistance against (5/8, 62.5%) antibiotics tested against the pathogenic bacteria. Contamination of vegetables by a range of pathogenic bacteria in local and super market vegetables is a serious threat to public health if they are consumed raw or unprocessed. Higher antibiotic resistance in pathogens isolated form supermarket vegetables needs to be investigated in order to monitor and control spread of infections with drug resistant bacteria. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22755 Stamford Journal of Microbiology, Vol.4(1) 2014: 13-18

scholarly journals Comparative study on the microbiological quality of vegetables collected from local markets and super shops of Dhaka city

2021 ◽  
Vol 11 (1) ◽  
pp. 24-27
Author(s):  
Mofijur Rahman Mamum ◽  
Kamal Kanta Das ◽  
Md Sohel Rana ◽  
Halimatus Sadia Tanim ◽  
Farahnaaz Feroz
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Local Market ◽  
Salmonella Spp ◽  
Pseudomonas Spp ◽  
Vegetable Samples ◽  
Lady’S Finger ◽  
Bacteria And Fungi

Vegetables are major source of vitamins and fibers but presence of pathogens in salad vegetables can cause outbreak of diseases. Several studies have been conducted on commonly consumed vegetables so far to detect the level of microbial contamination. However, few of them compare the microbial quality of local and super shop vegetables. The present study was conducted for microbiological assessment and comparing four types of vegetables collected from two different market conditions. In this study, Green chili (Capsicum frutescens L.), Tomato (Solanum lycopersicum), Coriander (Coriandrum sativum) and Lady’s finger (Abelmoschus esculentus) were analyzed to determine Total Viable Bacterial (TVB) Counts, Total Fungal (TF) Counts, Total Coliform Counts (TCC), Total Fecal Coliform (TFC) Counts and occurrence of Staphylococcus aureus, Pseudomonas spp., Listeria spp., Bacillus spp., Salmonella spp., and Vibrio spp. Among these microorganisms, Klebsiella spp. and Pseudomonas spp. were significantly present in coriander of both local market and super shop. All pathogenic bacteria and fungi were found in huge amounts in the vegetable samples of the local market. Although some pathogenic bacteria and fungi were also found in the same samples of the super shop, relatively lower than the local market’s vegetables. In conclusion, contamination in collected vegetable samples were not acceptable but comparatively higher load in local market samples indicated that the hygienic condition in these markets is not sufficiently maintained. Stamford Journal of Microbiology, Vol.11 (1) 2021: 24-27

Comparing antibiotic resistance in commensal and pathogenic bacteria isolated from wild-caught South Carolina shrimps vs. farm-raised imported shrimps

2007 ◽  
Vol 53 (7) ◽  
pp. 919-924 ◽  
Author(s):  
Kavitha Boinapally ◽  
Xiuping Jiang
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
South Carolina ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Vibrio Vulnificus ◽  
Resistant Bacteria ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Plate Counts

The objective of this study was to assess and differentiate wild-caught South Carolina (SC) shrimps from imported shrimps on the basis of microbiological analysis. Seven wild-caught SC shrimp and 13 farm-raised imported shrimp samples were analyzed. Total plate counts from wild-caught shrimp samples ranged from 4.3 to 7.0 log10 CFU/g, whereas counts from imported shrimp samples ranged from 3.2 to 5.7 log10 CFU/g. There was no difference (P > 0.05) between total bacterial counts of wild-caught SC shrimp and farm-raised imported shrimp. However, the percentages of bacteria with reduced susceptibility towards ceftriaxone and tetracycline were higher (P < 0.05) for farm-raised shrimp than for wild-caught samples. Salmonella spp. detected only in one farm-raised sample was resistant to ampicillin, ceftriaxone, gentamicin, streptomycin, and trimethoprim. Vibrio vulnificus was detected in both wild-caught and farm-raised shrimp samples; however, only the isolate from farm-raised shrimp was resistant to nalidixic acid and trimethoprim. Escherichia coli detected in one wild-caught sample was resistant to ampicillin. Both Listeria spp. and Salmonella spp. were absent with wild-caught SC samples. Therefore, the presence of more ceftriaxone- and tetracycline-resistant bacteria and the observed antimicrobial resistance phenotypes of isolates from the imported shrimp may reflect the possible use of antibiotics in raising shrimp in those countries.

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

2019 ◽  
Vol 36 (1) ◽  
pp. 49-53
Author(s):  
Kayseri Afroz ◽  
Tahmina Shammi ◽  
Md Shahidul Kabir
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Antibiotic Sensitivity ◽  
Multi Drug Resistance ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
Shigella Spp ◽  
Vibrio Spp

Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53

scholarly journals Microbiological Quality Assessment of Marketed Broiler Meat in Different Markets of Dhaka City

2020 ◽  
Vol 7 (2) ◽  
pp. 261-266
Author(s):  
Mst Tasmim Sultana ◽  
Ashrifa Akter Mukta ◽  
Lita Biswas ◽  
Md Masud Rana
Keyword(s):  
Research Work ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Total Coliform ◽  
Viable Count ◽  
Resistant Bacteria ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Meat Samples

The present research work was undertaken to assess the bacterial quality and to know the prevalence of zoonotic bacteria from broiler meat samples sold in Krishi market, Bihari camp market, Agargaon market, Taltola market and SAU (Sher-e-Bangla Agricultural University) mini bazar, Dhaka, Bangladesh from January to May 2018. After processing of samples primary culture was done in nutrient broth and nutrient agar media then pure culture was obtained from different selective media. Total Viable Count (TVC), Total Coliform Count (TCC) and Total Salmonella Count (TSC) in broiler meat of different broiler markets were determined. Mean of TVC, TCC and TSC for the Krishi market, Agargoan market, Taltola market, Bihari camp market and SAU Mini market were 5.67, 4.32, 2.96 log10 CFU/g, 5.88, 4.64, 3.56 log10 CFU/g, 6.10, 4.68, 3.78 log10 CFU/g, 6.68,4.87, 3.84 log10 CFU/g and 5.84, 4.25, 3.13 log10 CFU/g respectively. The prevalence of Escherichia coli and Salmonella spp. Were 74% and 42% respectively. E. coli isolates were showed sensitive to Ciprofloxacin (91.6%), Gentamycin (87.5%), Azithromycin (66.66%), and Tetracycline (58%) and resistant to Penicillin (79.16%) then Amoxycillin (75%), Streptomycin (75%) and Ampicillin (58.3%). Highest resistant pattern was showed by Tetracycline (58%), Streptomycin (72.72%), Penicillin (72.72%) and Amoxycillin (63.63%). Highest resistant pattern showed by Amoxycillin (71.42%) and Penicillin (71.42%). This study revealed that broiler meat sold at some local markets of Dhaka city were contaminated with multiple species of multidrug resistant bacteria which may risk for human health. Res. Agric., Livest. Fish.7(2): 261-266,  August 2020

scholarly journals Assessment of the microbiological quality of beverages sold in collective cafes on the campuses of the University of Abomey-Calavi, Benin Republic

2020 ◽  
Author(s):  
Gwladys S. Komagbe ◽  
Philippe Sessou ◽  
François Dossa ◽  
Prudencio Sossa-Minou ◽  
Bernard Taminiau ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Compliance Rate ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Human Beings ◽  
Salmonella Spp ◽  
Potential Health ◽  
Antibiotic Susceptibility Test ◽  
Aerobic Flora

Fresh juices are highly nutritious foods for human beings, but the inability to observe requirements for their preparation, packaging and storage subjects them to microbial contamination which poses a potential health risk to consumers. The purpose of this study was to evaluate the microbiological quality of beverages sold within the cafes of the campuses of Abomey-Calavi University (Benin). A survey carried out among beverage vendors showed that the sources of contamination were uncontrolled and the raw materials used were of questionable quality as the operators lacked good hygienic practices. Thus, the microbial quality of forty-five samples of four types of beverages sold in these cafes was investigated for mesophilic aerobic flora, Coliforms, Escherichia coli, Staphylococcus aureus, sulfate-reducing anaerobic spores, fungal flora and Salmonella spp. using standardized methods. Then, molecular studies identified the pathogenic strains isolated from the beverages. An antibiotic susceptibility test was performed on the strains identified for the detection of multi-resistant bacteria. These analyses revealed a non-compliance rate of 100% in the analyzed samples. The indicators that caused this non-compliance in the samples were mesophilic aerobic flora, coliforms and fungi. In addition, 85.7% of the samples contained other Enterobacteriaceae including Klebsiella pneumoniae, Morganella morganii, Kluyvera georgiana, Citrobacter murliniae, Yersinia intermedia. While the non-compliance rates of the samples for Salmonella spp and E.coli were 4.4% each, the non-compliance rate for S. aureus was 2.2% with the presence of sometimes multi-resistant pathogenic bacteria. Sellers' awareness of good hygiene practices is important for improving the quality of food sold.

Assessment of the Microbiological Quality of Ready-To-Use Vegetables for Health-Care Food Services

1997 ◽  
Vol 60 (8) ◽  
pp. 954-960 ◽  
Author(s):  
JOSEPH A. ODUMERU ◽  
SHEILA J. MITCHELL ◽  
DAVID M. ALVES ◽  
JOHN A. LYNCH ◽  
ARLENE J. YEE ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Food Services ◽  
Salmonella Spp ◽  
E Coli ◽  
Vegetable Samples ◽  
Serotype O ◽  
Select Group ◽  
Before And After

The microbiological quality of ready-to-use (RTU) vegetables, including chopped lettuce, salad mix, carrot sticks, cauliflower florets, sliced celery, coleslaw mix, broccoli florets, and sliced green peppers was determined before and after processing. Microbial profiles were obtained 24 h after processing and on days 4, 7, and 11 after storage at 4 and 10°C to simulate temperature abuse. In addition, the microbial profiles of four RTU vegetables, coleslaw mix, salad mix, cauliflower florets, and sliced green peppers were determined 7 days after distribution to a select group of Ontario hospitals. RTU vegetables, with the exception of green peppers, showed up to a 1-log decrease in aerobic colony counts after processing. These counts increased to preprocessing levels after 4 days of storage at both 4 and 10°C. RTU vegetables stored at temperature abuse conditions (10°C)had significantly higher counts (P &lt; 0.001) on days 4 to 11 as compared to those stored at 4°C. Green peppers had the highest bacterial counts while cauliflower and chopped lettuce had the lowest counts at both storage temperatures (P &lt; 0.05). Increased levels of Listeria monocytogenes in RTU vegetables were associated with temperature abuse. Levels of &gt;100 MPN/g for L. monocytogenes were detected in 8 of 120 (6.7%) samples stored at 10°C but not in 175 samples stored at 4°C after 7 days (P &lt; 0.05). Overall, L. monocytogenes was detected in 13 of 120 (10.8%) RTU vegetables stored for up to 11 days at 10°C and 5 of 176 (2.8%) samples stored at 4°C (P &lt; 0.05). E. coli was detected in 2 of the 120 (1.7%) processed RTU vegetables after day 7 of storage at 10°C and 1 of the 65 (1.5%) unprocessed vegetables from the same batches of vegetables used for processing. This indicator organism was not detected in RTU vegetable samples stored at 4°C or in any of the RTU vegetable samples obtained from hospital coolers. Other pathogenic bacteria, such as Salmonella spp., Campylobacter spp., Yersinia enterocolitica (serotype O:3) and verocytotoxigenic E. coli (VTEC) were not detected in any of the RTU vegetables tested, Recommendations regarding processing, distribution, and storage of these products are presented.

scholarly journals Antibiotic resistance pattern of bacteria isolated from outdoor patients in Dhaka city: a single center study

2020 ◽  
Vol 9 (1) ◽  
pp. 1-4
Author(s):  
Most Salma Khatun ◽  
Saimun Nahar ◽  
Md Shahidul Kabir
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Age Groups ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Dhaka City ◽  
Female Patients

Bacterial infections are often found to cause morbidity and mortality around the globe. Indiscriminate use of antibiotic for treatment of such infections is reported to cause selective pressure and increase in drug resistance. Emergence of antibiotic resistance is a growing concern for people of all age having bacterial infections. It is important to determine the trend of antibiotic resistance patterns of pathogenic bacteria isolated from clinical samples for appropriate treatment of patients. A retrospective study was conducted on patient samples collected from 1 July 2018 to 15 March 2019. A total of 500 urine, 136 blood, 120 stool, 172 swab, 90 Sputum and 60 pus samples were randomly collected from both male and female patients of different age groups who reported to a diagnostic centre in Dhaka city. Pathogenic bacteria were isolated and identified from the collected samples following standard methods. Antibiotic sensitivity patterns of isolated bacteria were also determined by disc diffusion test. Proportion of UTI in female patients (71.67%) was found to be higher than the male (28.33%) patients and mostly caused by Escherichia coli (73.33%). Among Gram negative bacteria, E. coli (51%) was found to be predominant followed by Pseudomonas spp. (11.47%), Klebsiella (9.84%) and Salmonella Typhi (9.84%). Among Gram positive organism Staphylococcus aureus (9.0%), beta haemolytic streptococci (5.74%) and enterococci (3.28%) were found to be present. Among the tested antibiotics imipenem was found to be the most effective (93.02%) followed by gentamycin (76.03%) against all isolated bacteria. Amoxycillin was found to be least effective (21.29%) against all isolated bacteria. Increase in antibiotic resistance was possibly due to indiscriminate use of antibiotic for treatment of various infections. Prudent use of antimicrobial drugs will help reduce spread of resistant bacteria and complication of treatment of infected patients. Stamford Journal of Microbiology, Vol.9(1) 2019: 1-4

scholarly journals Assessing the Molecular Targets and Mode of Action of Furanone C-30 on Pseudomonas aeruginosa Quorum Sensing

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1620
Author(s):  
Victor Markus ◽  
Karina Golberg ◽  
Kerem Teralı ◽  
Nazmi Ozer ◽  
Esti Kramarsky-Winter ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Quorum Sensing ◽  
Conformational Changes ◽  
Mode Of Action ◽  
Pathogenic Bacteria ◽  
Molecular Targets ◽  
Resistant Bacteria ◽  
Public Healthcare ◽  
C 30

Quorum sensing (QS), a sophisticated system of bacterial communication that depends on population density, is employed by many pathogenic bacteria to regulate virulence. In view of the current reality of antibiotic resistance, it is expected that interfering with QS can address bacterial pathogenicity without stimulating the incidence of resistance. Thus, harnessing QS inhibitors has been considered a promising approach to overriding bacterial infections and combating antibiotic resistance that has become a major threat to public healthcare around the globe. Pseudomonas aeruginosa is one of the most frequent multidrug-resistant bacteria that utilize QS to control virulence. Many natural compounds, including furanones, have demonstrated strong inhibitory effects on several pathogens via blocking or attenuating QS. While the natural furanones show no activity against P. aeruginosa, furanone C-30, a brominated derivative of natural furanone compounds, has been reported to be a potent inhibitor of the QS system of the notorious opportunistic pathogen. In the present study, we assess the molecular targets and mode of action of furanone C-30 on P. aeruginosa QS system. Our results suggest that furanone C-30 binds to LasR at the ligand-binding site but fails to establish interactions with the residues crucial for the protein’s productive conformational changes and folding, thus rendering the protein dysfunctional. We also show that furanone C-30 inhibits RhlR, independent of LasR, suggesting a complex mechanism for the agent beyond what is known to date.

scholarly journals Bacteria and Their Antibiotic Resistance Profiles in Ambient Air in Accra, Ghana, February 2020: A Cross-Sectional Study

2021 ◽  
Vol 6 (3) ◽  
pp. 110
Author(s):  
Godfred Saviour Kudjo Azaglo ◽  
Mohammed Khogali ◽  
Katrina Hann ◽  
John Alexis Pwamang ◽  
Emmanuel Appoh ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Ambient Air ◽  
Cross Sectional Study ◽  
Bacillus Species ◽  
Resistant Bacteria ◽  
Cross Sectional ◽  
Antibiotic Resistant ◽  
Bacterial Counts ◽  
Inappropriate Use

Inappropriate use of antibiotics has led to the presence of antibiotic-resistant bacteria in ambient air. There is no published information about the presence and resistance profiles of bacteria in ambient air in Ghana. We evaluated the presence and antibiotic resistance profiles of selected bacterial, environmental and meteorological characteristics and airborne bacterial counts in 12 active air quality monitoring sites (seven roadside, two industrial and three residential) in Accra in February 2020. Roadside sites had the highest median temperature, relative humidity, wind speed and PM10 concentrations, and median airborne bacterial counts in roadside sites (115,000 CFU/m3) were higher compared with industrial (35,150 CFU/m3) and residential sites (1210 CFU/m3). Bacillus species were isolated in all samples and none were antibiotic resistant. There were, however, Pseudomonas aeruginosa, Escherichia coli, Pseudomonas species, non-hemolytic Streptococci, Coliforms and Staphylococci species, of which six (50%) showed mono-resistance or multidrug resistance to four antibiotics (penicillin, ampicillin, ciprofloxacin and ceftriaxone). There was a positive correlation between PM10 concentrations and airborne bacterial counts (rs = 0.72), but no correlations were found between PM10 concentrations and the pathogenic bacteria nor their antibiotic resistance. We call for the expansion of surveillance of ambient air to other cities of Ghana to obtain nationally representative information.

Sign in / Sign up

Export Citation Format

Share Document