scholarly journals Microbiological analysis of ready to eat foods collected from different places of Dhaka city, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 30-33
Author(s):  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
French Fries ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Fish Fillet ◽  
Bacillus Spp

The current study was attempted to observe the microbiological quality of ready to eat foods collected from different places of Dhaka city, Bangladesh. Ten food samples that include five fried items-fried chicken, fish fillet, shrimp fries, French fries, potato chop and five sweet items-yoghurt, pudding, ice cream, mango drink, lemon drink were analyzed during the period from September 2017 to November 2017. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. The higher counts of TVBC, Escherichia coli, Staphylococcus aureus and Bacillus spp. were recorded as 1.94×106 cfu/g (potato chop), 3.1×104 cfu/g (fried shrimp), 7.5×103 cfu/g (fried chicken) and 1.0 x 103 cfu/g (French fries) respectively. Based on the specifications by International Commission for Microbiological Specification for Foods (ICMSF), the level of contaminations was within acceptable microbiological limits except for potato chop. Stamford Journal of Microbiology, Vol.8(1) 2018: 30-33

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Microbiological Analysis of Zobo Drink Preserved with Scent Leaves (Ocimum gratissimum)

2020 ◽  
pp. 1-10
Author(s):  
Chukwuma G. Udensi ◽  
Ugonna D. Nwankpa ◽  
Emmanuel K. Amanze ◽  
Chibuzor V. Nwokafor ◽  
Chinedu E. Udekwu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Microbiological Analysis ◽  
Microbial Counts ◽  
Sanitary Condition ◽  
Bacillus Spp ◽  
Penicillium Spp

Aim: To determine the microbiological quality of zobo drink preserved with scent leaves. Methods: The zobo drink and scent leaves were prepared and evaluated using standard microbiological techniques. Results: Twenty three (23) bacteria species and fourteen (14) fungi species were identified from zobo drink preserved with scent leaves samples. This reveals the major bacterial species to be Enterobacter spp, Staphylococcus aureus, Bacillus spp, and Micrococcus spp. and fungi species to be Aspergillus niger, Rhizopus spp and Penicillium spp. The bacterial and fungal counts decreased as the days increased with day 1 having the highest bacterial and fungal counts at 1.41x105 (cfu/ml) and 3.1x104 (cfu/ml) respectively. The control samples were generally higher than the counts recorded on the bacterial and fungal counts. Zobo + scent leaves (ZSC) recorded the highest bacterial count at 1.41x105 (cfu/ml), while the least was recorded for (ZSA) at 1.01x106 (cfu/ml). Zobo + Scent (ZSC) recorded the highest fungal counts at 3.1x104 (cfu/ml), while the least was recorded for ZSA at 1.2x105 (cfu/ml). From this study, Bacillus spp and Staphylococcus aureus were the most frequently occurring bacterial isolates with a high percentage occurrence of 8(21.6%) and 6(16.2%), while Penicillium spp was the most frequently occurring fungal isolate. Conclusion: The association of these microorganisms with foods such as the commercial zobo drinks may be as a result of poor hygiene or poor sanitary condition. The microbial counts showed that among the zobo drink preserved with scent leaves samples, zobo + scent leaves (ZSC) is the most predisposed product to microbial population due to the high microbial counts recorded. Therefore, the result revealed that the samples of zobo drink were directly and indirectly contaminated with high levels of pathogenic bacteria, but can be reduced by the addition of scent leaves as a preservative.

scholarly journals Microbiological analysis and detection of anti-bacterial activity of Centella asiatica and Aloe vera samples collected from different areas of Dhaka city, Bangladesh

2017 ◽  
Vol 6 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Susmita Bhowmik ◽  
Runa Akter Chowdhury ◽  
Md Aftab Uddin
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Centella Asiatica ◽  
Bacterial Count ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Fungal Load ◽  
Bacillus Spp ◽  
Klebsiella Spp

The present study was performed to detect the presence of contaminating microorganisms in two commonly available herbal samples (Centella asiatica and Aloe vera) collected from different areas of Dhaka city, Bangladesh and to assess their antibacterial activity. Out of twenty samples (having ten samples of each categories) studied; the range of total viable bacterial count was approximately 103 to 108 cfu/g. Presence of Staphylococcus aureus was found in all the samples, followed by Klebsiella spp. in 15 samples, Pseudomonas spp. in 14 samples, Bacillus spp. in 12 samples, Escherichia coli in 9 samples and Vibrio spp. in 7 samples. Salmonella spp. was detected in neither of the sample. 17 samples showed a high fungal load up to 107 cfu/g. Antibacterial activity of C. asiatica samples was demonstrated against eight laboratory isolates. Only four C. asiatica samples showed activity against Klebsiella spp. On the contrary, Aloe vera samples (12-14) showed antibacterial activity only against Staphylococcus spp.Stamford Journal of Microbiology, Vol.6(1) 2016: 39-43

scholarly journals Microbiological analysis of commonly used toothpaste samples in Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 38-40
Author(s):  
Mousumi Talukder ◽  
Ifra Tun Nur
Keyword(s):  
Microbiological Quality ◽  
Enteric Bacteria ◽  
Fungal Species ◽  
Microbiological Analysis ◽  
Bacillus Spp ◽  
Viable Bacteria ◽  
Dental Diseases ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Great Public Health

Present study attempted to identify and enumerate microorganisms spoiling commonly used toothpaste samples. Among 7 brand of toothpaste, almost all were found to be rigorously contaminated with total viable bacteria within a range of 103 – 105 cfu/g. Proliferation of fungal species was observed up to 105 cfu/g. Prevalence of Staphylococcus spp., Pseudomonas spp. and Bacillus spp. was observed within a range of 103-105 cfu/g while Vibrio spp. were completely absent. Among the enteric bacteria, Escherichia coli and Klebsiella spp. was found in all the samples tested. Such findings highlighted a great public health risk associated with dental diseases among the users and thereby specified the importance to introduce a proper guideline in maintaining good microbiological quality for such daily usage healthcare products. Stamford Journal of Microbiology, Vol.8(1) 2018: 38-40

Microbiological quality and safety of Ready- to- eat foods from restaurants and food establishments in Yirgalem town Southern, Ethiopia

2019 ◽  
Author(s):  
Tsegaye Shamebo Arficho ◽  
Asefa Hamato Kebede
Keyword(s):  
Bacterial Load ◽  
Microbiological Quality ◽  
Food Samples ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Southern Ethiopia ◽  
Microbiological Analysis ◽  
Quality And Safety ◽  
Mannitol Salt Agar ◽  
Microbiological Methods

Abstract Background: Foodborne illnesses are considered as one of the most important public health problems particularly in developing countries like Ethiopia. This study aimed to determine the microbiological quality and safety of ready-to-eat foods in Yirgalem town, southern Ethiopia from November 2016 to August 2017. Methods: The collection of ready-to-eat food samples and laboratory-based microbiological analysis was used as the study design. A total of 160 food samples comprising of 40 ‘Injera firfir’, 40‘Bayeaynet’, 40 Vegetables and 40 Spaghetti were collected and analyzed for microbial contamination following standard microbiological methods. Ten grams of each food sample was transferred into 90 ml of buffered peptone water and homogenized for 5 minutes using a vortex mixer. The homogenates were serial diluted up to 10-7 and a volume of 0.1ml aliquot was spread plated on pre-solidified media of Aerobic plate count agar, MacConkey agar, Mannitol salt agar, and Salmonella-Shigella agar and incubate at 35-37oc for 24 hrs. Also, Potato Dextrose Agar was used for the isolation of fungi. Data were entered into Microsoft Excel and analyzed using SPSS version 20.0. Results: All the collected food samples were subjected to total aerobic mesophilic bacteria, Coliform bacteria, Enterobacteriaceae, Staphylococcal, Yeasts, and Molds counts. Accordingly, the mean counts expressed as log10 CFU/g of food for each group of the organism were 7.90 ± 0.71, 4.31±1.30, 4.32 ± 1.30, 6.70 ± 0.34 and 4.5 ± 1.01, respectively. The highest bacterial load 162(28.9%) was detected in ‘Injera firfir’ whereas the lowest 108(19.2%) case was investigated in Spaghettis. Regarding the food safety issue, the frequency of S. aureus, E. coli and Salmonella spp in the food samples were 54.4%, 43.8%, and 0.6%, respectively. Conclusion: The high microbial load and existence of foodborne pathogens in ready-to-eat foods in Yirgalem town, Southern Ethiopia is calling for the creation of awareness among restaurant and food establishment owners and food handlers concerning the hygienic practice. Keyword: Microbial quality, Yirgalem town, Southern Ethiopia

scholarly journals Bacterial Contamination of Street Vended Food Pani Puri Available in Janakpurdham, Dhanusha

2019 ◽  
Vol 6 ◽  
pp. 70-75
Author(s):  
Nagendra Prasad Yadav ◽  
Rakesh Kumar Yadav ◽  
Satyam Prakash
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Bacterial Contamination ◽  
Food Samples ◽  
Microbial Count ◽  
Microbiology Laboratory ◽  
Dilution Technique ◽  
Bacillus Spp ◽  
Klebsiella Spp

Objectives: To determine the level of bacterial contamination in food (Panipuri) available in market of Janakpur, Dhanusha, Nepal. Methods: Total 120 samples of Panipuri (60 solid matters and 60 masalaa Pani) collected aseptically from the market were transported to the microbiology laboratory and processed for microbial count by serial dilution technique. Microbial load and presence of pathogen were detected in each sample. Results: Analysis of the food samples revealed that 70% of Panipuri samples had high loads of bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella spp, Pseudomonas spp, Bacillus spp.  Conclusion: This study depicted that the street vended food Panipuri of Janakpur are highly contaminated with pathogenic bacteria which can cause health risk to consumers. Thus, it is suggested that regular monitoring of the quality of street foods must be practiced to avoid any food bone illness in future.

Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa

1999 ◽  
Vol 62 (11) ◽  
pp. 1278-1284 ◽  
Author(s):  
FRANCINA M. MOSUPYE ◽  
ALEXANDER von HOLY
Keyword(s):  
South Africa ◽  
Foodborne Pathogens ◽  
Water Samples ◽  
Microbiological Quality ◽  
Food Samples ◽  
Quality And Safety ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in Johannesburg, South Africa. Food temperatures were recorded at the time of sampling. Standard methods were used to determine aerobic plate counts (APCs), spore counts (SCs), and Enterobacteriaceae counts (ECs) for food samples as well as coliform counts (CCs) for water and swab samples. In addition, Petrifilm Escherichia coli count (PC) plates were used for the enumeration of coliforms in food, water, and swab samples. The presence of selected foodborne pathogens in the food samples as well as the presence of nonpathogenic E. coli 1 (in food and water samples) was also tested for. Predominant colonies isolated from APC plates were characterized to the genus level. Holding temperatures for cooked meats and gravies ranged from 42.0 to 94.0°C, and those for uncooked salads ranged from 29.0 to 39.0°C. Mean APC values of 3.4 (±0.4) log CFU/g, 4.0 (±1.2) log CFU/ml, and 2.1 (±0.4) log CFU/25 cm2 were obtained for food, water, and swab samples, respectively. Mean SC values of 1.6 (±0.2) log CFU/g and 1.5 (±0.3) log CFU/25 cm2 were obtained for food and swab samples, respectively. A mean EC value of 2.0 (±0.4) log CFU/g for food samples and mean CC values of 2.5 (±0.3) log CFU/ml and 1.3 (±0.3) log CFU/25 cm2 for water and swab samples, respectively, were determined. Mean PC values of 1.6 (±0.1) log CFU/g, 1.9 (±0.6) log CFU/ml, and 1.4 (±0.4) log CFU/25 cm2 were determined for food, water, and swab samples, respectively. Bacillus cereus was detected in 22%, Clostridium perfringens in 16%, Salmonella spp. in 2%, and E. coli (non-O157:H+) in 2% of the 51 food samples. E. coli was found in 14 water samples (78%) and in 3 food samples (6%). Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, and Yersinia enterocolitica were also tested for in the food samples, but they were not detected. The 340 isolates obtained from APC plates for food, water, and swab samples were predominantly Bacillus spp., Micrococcus spp., and Staphylococcus spp. for all three sample types. It was concluded that the foods analyzed in this study were of acceptable quality and safety.

scholarly journals Microbiological Analysis of Pharmaceutical Non Injectable Drugs Produced in Dhaka, Bangladesh

2020 ◽  
Vol 5 (2) ◽  
pp. 1-9
Author(s):  
Ahmed T
Keyword(s):  
Antibacterial Activity ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Microbiological Analysis ◽  
Pharmaceutical Drugs ◽  
Injectable Drugs ◽  
Three Samples ◽  
Fungal Load ◽  
States Pharmacopeia

Pharmaceutical drugs are applied and consumed by the patients with weak immune system and for this reason these products must be of good quality and within the required microbiological limit. Present study attempted to determine the microbiological quality of pharmaceutical non injectable oral and topical drugs as well as their antibacterial activity. A total of sixty samples were studied from different categories of medicine including syrup, tablet & capsule and ointments. Microbiological analysis was done after serial dilution. Antibacterial activity of the samples was also determined by Kirby-Bauer method. The total viable bacterial count of 9 syrups, 7 tablet & capsules and 13 ointments samples exceeded the microbial limit ˂102cfu/ml or cfu/gm recommended by USP (United States Pharmacopeia) and BP (British Pharmacopeia). Regarding to the presence of specific bacteria, about six, six and three samples from syrup, tablet & capsule and ointment samples were of good quality respectively out of twenty samples each. Some drug prevailed good activity towards few bacteria and no activity at all to some others. As the drugs possess antibacterial activity, the contaminants might represent some other species of the same genera of bacteria having some mechanisms to prohibit such activities towards them. More than 50% of the drugs contain higher bacterial and fungal load rendering the quality at risk and not recommended to use by the patients to whom these products will impart most harm as these patients are already immune compromised.

scholarly journals Microbiological quality and somatic cells of in natura milk produced in Alagoas State, Brazil

2020 ◽  
Vol 9 (9) ◽  
pp. e412997379
Author(s):  
Bruno Fernando Oliveira Araújo ◽  
Sybelle Geórgia Mesquita da Silva ◽  
João Manoel da Silva ◽  
Cícero Cerqueira Cavalcanti Neto ◽  
Paula Cibelly Vilela da Silva ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Economic Value ◽  
Somatic Cells ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Microbiological Analysis ◽  
Salmonella Spp ◽  
Psychrotrophic Bacteria ◽  
Physical Chemical ◽  
Significant Difference

Raw milk is a food with great consumption and economic value in Brazil. However, is susceptible of contamination by pathogenic bacteria. The aimed of this study was to evaluate the quality of in natura milk based on microbiological in three dairy farms, somatic cells counting (SCC), bacterial counting and his physical-chemical composition. Were made the following microbiological analysis: counting of mesophilic and psychrotrophic bacteria, coliforms at 30 ºC, coliforms at 45 ºC, Staphylococcus spp., Listeria spp., and SCC. The physical-chemical analysis was fat, protein, lactose, total solids, urea, and casein. There was no evidence of Salmonella spp. and Escherichia coli were identified in any samples.  In accordance to the microbiological standards established by Normative Instruction 76 only coliforms 30 ºC and 45 ºC counts were above the standards. There was a significant difference (p≤0.05) between the three farms studied regarding most microbiological aspects. Also, was observed difference (p≤0.05) for most of physical-chemical aspects. Overall, the milk produced in the regions of Alagoas State fails to meet just a constant criterion in the current legislation.

scholarly journals Microbiological quality analysis of commercial fruit juice in Dhaka City, Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 15-18
Author(s):  
Md Al Amin ◽  
Mofijur Rahman Mamun ◽  
Kamal Kanta Das
Keyword(s):  
Fruit Juice ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Quality Analysis ◽  
Dhaka City ◽  
Food Borne ◽  
Good Manufacturing Practices ◽  
High Bacterial Count ◽  
Staphylococcus Spp

The aims of this study were to evaluate the microbiological quality of different commercially available fruit juice of Dhaka city. In this study total 20 fruit juice samples of five different types were repeatedly subjected to bacteriological and mycological screening for three months. Isolates were identified and confirmed using cultural and biochemical characteristics. Current study showed high bacterial and fungal loads consisting of specific pathogens such as coliform and Staphylococcus spp., fecal coliform and other pathogens are totally absent. The presence of Staphylococcus aureus portends health risk to consumers as some species produce potent toxins associated with food borne illnesses. And high bacterial count indicated unhygienic conditions of the processing area, and that good manufacturing practices during processing are not maintained properly. Recommendations are required to reduce the microbiological contamination and promote quality assurance of the products. Stamford Journal of Microbiology, Vol.8(1) 2018: 15-18

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