scholarly journals Characterization of Antimicrobial, Antioxidant, Anticancer Property and Chemical Composition of Michelia champaca Seed and Flower Extracts

1970 ◽  
Vol 4 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Lee Seong Wei ◽  
Wendy Wee ◽  
Julius Young Fu Siong ◽  
Desy Fitrya Syamsumir
Keyword(s):  
Antimicrobial Property ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Anticancer Activities ◽  
E Coli ◽  
Flower Extract ◽  
Microdilution Method ◽  
Ic50 Value ◽  
Michelia Champaca

This study was carried out to characterize antimicrobial, antioxidant and anticancer activities of Michelia champaca seed and flower extracts. The main objective of the present study was to reveal the medicinal values of M. champaca seed and flower for human uses. Antimicrobial property of M. champaca seed and flower extracts were revealed by using two fold microdilution method whereas antioxidant activity of the extract was determined with DPPH radical scavenging method. The anticancer property of the plant extract was revealed through Colorimetric MTT (tetrazolium) assay. The minimum inhibitory concentration values of M. champaca seed and flower extracts ranged from 15.6 to 125mg/l and 7.8 to 62.5mg/l, respectively in which both of the plant extracts were found can inhibit the growth of all the tested bacterial isolates namely A. hydrophila, E. tarda, E. coli, Flavobacterium sp., Klebsiella sp., P. aeruginosa, Salmonella sp., V. alginolyticus, V. cholerae and V. parahaemolyticus. M. champaca flower extract was able to control the growth of E. tarda, E. coli, Flavobacterium sp., P. aeruginosa and V. cholerae at the concentration of 7.8mg/l whereas A. hydrophila, Klebsiella sp. and V. alginolyticus were failed to grow at the concentration 15.6mg/l. The M. champaca flower extract was also able to control the growth of Salmonella sp. and V. parahaemolyticus at the concentration of 62.5mg/l. At the maximum concentration of M. champaca seed and flower extracts were found can inhibit only 40% of DPPH whereas the IC50 value of M. champaca seed and flower extract against MCF-7 cells was 1.98 ±0.31μg/ml and 1.86 ± 0.21μg/ml, respectively. A total of 9 chemical compounds were successfully identified in M. champaca’s flower extract whereas 37 chemical compounds were found in the leaf extract. The findings of the present study indicated that medicinal values of M. champaca seed & flower extracts in terms of antimicrobial and anticancer are promising.Key words: Antioxidant; Anticancer; Antimicrobial; Chemical compound; Michelia champaca DOI: http://dx.doi.org/10.3329/sjps.v4i1.8862 SJPS 2011; 4(1): 19-24

scholarly journals ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF CITRUS LEMON PEELS ENCAPSULATED IN PVA

2019 ◽  
pp. 110-125
Keyword(s):  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Alginate Beads ◽  
E Coli ◽  
Lemon Peel ◽  
Ic50 Value ◽  
Lime Peel ◽  
Percentage Release ◽  
Infrared Techniques

In this study, waste lemon peels were converted into a dietary supplement. Lemon peels were used because of the abundance of phytochemicals present in it and also they are easily available throughout the year. To improve the therapeutic efficacy, we used Polyvinyl Alcohol (PVA) as a nanocarrier of lemon peel methanolic extract. The lemon peel extract was encapsulated in PVA by the solvent evaporation method, to improve the solubility and stability of the compounds in the extract. Characterization of the prepared lime peel nanoformulation (LP-NF) was done by Scanning Electron Microscope, Zeta potential and Fourier Transform Infrared techniques. The antioxidant assays like DPPH(2,2-diphenyl-1- picrylhydrazyl) radical scavenging assay and hydrogen peroxide assay showed a high scavenging activity when compared with commercial supplement with the IC50 value of 24 ± 0.05 and 26.07 ± 0.11 respectively. The Gram-negative bacteria, E. coli showed a zone of inhibition of 18 mm indicating the antibacterial property of LP-NF. The percentage release of the nanoformulation from sodium alginate beads was calculated and it showed the release of nanoparticle up to 83% after 7 hours in PBS at pH 7.4

scholarly journals Preparation and Characterization of Chitosan Nanoparticles-Doped Cellulose Films with Antimicrobial Property

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Ain Nadirah Binti Romainor ◽  
Suk Fun Chin ◽  
Suh Cem Pang ◽  
Lesley Maurice Bilung
Keyword(s):  
Antimicrobial Activity ◽  
Citric Acid ◽  
Antimicrobial Agents ◽  
Antimicrobial Property ◽  
Chitosan Nanoparticles ◽  
Assay Method ◽  
E Coli ◽  
Cellulose Films ◽  
Diffusion Assay

Cellulose films with antimicrobial property were prepared by incorporation of chitosan nanoparticles as antimicrobial agents into the cellulose films. The antimicrobial property of these chitosan nanoparticles-doped cellulose films againstEscherichia coli(E. coli) was evaluated via diffusion assay method, minimum inhibitory concentration (MIC) method, and minimum bactericidal concentration (MBC) method. The effects of antimicrobial agent amount, size-related property (nanoparticles and bulk chitosan), and crosslinking by citric acid on antimicrobial activity of cellulose films were studied. It was observed that the antimicrobial activity was enhanced when chitosan nanoparticles were used as compared to when bulk chitosan was used. A maximumE. coliinhibition of 85% was achieved with only 5% (v/v) doping of chitosan nanoparticles into the cellulose films. Crosslinking of the cellulose films with citric acid was observed to have resulted in 50% reduction of water absorbency and a slight increase ofE. coliinhibition by 3% for chitosan nanoparticles-doped cellulose films.

scholarly journals Biochemical characterization of pink husked coconut types

2021 ◽  
pp. 72-76
Author(s):  
Regi J. Thomas ◽  
M. Shareefa ◽  
H. Harsha ◽  
Anitha Karun
Keyword(s):  
Biochemical Characterization ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenol Content ◽  
Anthocyanin Content ◽  
Scavenging Activity ◽  
Traditional Medicines ◽  
Ic50 Value ◽  
Morphological Differences

Coconuts with various traits are available in different coconut growing countries. The pink husk is one such trait that has already been reported in coconut. There is a demand for tender nut water from pink husked types of coconut to treat hepatitis by traditional medicinal practitioners. Present studies were carried out to characterize pink husked types identified in Guam Tall variety of coconut. There were no morphological differences with respect to the nut characters between pink and non-pink husked types. Biochemical characterization of pink husked types revealed significantly higher total phenol content in the tender nut water of pink husked types than normal husked types. A significantly higher protein content of 86 mg 100 mL-1 was noted in pink husked type, compared to normal husked type with 58.7 mg 100 mL-1. Higher free radical scavenging activity based on DPPH assay with pink husked type was recorded with an IC50 value of 266.7 compared to normal type with an IC50 value of 358. Similarly, phosphomolybdate assay also revealed higher scavenging activity of pink husked type based on the IC50 value of 415.2 compared to 637.9 observed in normal husked type. Anthocyanin content of 25.98 mg 100g-1 fresh weight was noted in the exocarp of pink husked types, and it was negligible in normal husked type. These results justify the use of pink husked coconuts in various traditional medicines. There is tremendous potential for exploiting pink husked types of coconut in the pharmaceutical industry.

scholarly journals POTENSI EKSTRAK ETANOL DAUN ANDONG MERAH (Cordyline fruticosa (L.) A. Cheval) SEBAGAI ANTIOKSIDAN PENANGKAL RADIKAL DPPH

2021 ◽  
Vol 4 (1) ◽  
pp. 24
Author(s):  
Yuri Pratiwi Utami
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Antioxidant Potential ◽  
Traditional Medicinal Plants ◽  
Ic50 Value ◽  
Cordyline Fruticosa

AbstractAndong leaf is one of the traditional medicinal plants that is proven to have various properties including as a (natural) medicinal ingredient. Andong plants contain several chemical compounds including saponins, tannins, flavonoids, polyphenols, steroids. The purpose of this study was to determine the potential of red andong leaf extract (Cordyline fruticosa (L.) A. Cheval) as a radical scavenging antioxidant for DPPH. In this study red andong leaves (Cordyline fruticosa (L.) A. Cheval) were extracted using maceration method with 70% ethanol as solvent. The results showed that anthocyanins were cyanidin. Antioxidant activity was measured by reducing DPPH free radicals with UV-Vis spectrophotometry at a wavelength of 517 nm. The results showed that red andong red leaf extract had strong antioxidant potential with an IC50 value of 64.5197 g/mL against free radicals DPPH and vitamin C as a comparison showed a very strong antioxidant potential with an IC50 value of 2.12 g/mL.Keywords : Andong leaf, antioxidant, DPPH AbstrakDaun andong merupakan salah satu tanaman obat tradisional yang terbukti memiliki berbagai khasiat diantaranya sebagai bahan obat (alami). Tanaman andong mengandung beberapa senyawa kimia diantaranya yaitu saponin, tanin, flavonoid, polifenol, Steroida. Tujuan dari penelitian ini adalah untuk mengetahui potensi  ekstrak daun andong merah (Cordyline fruticosa (L.) A. Cheval) sebagai antioksidan penangkal  radikal DPPH. Pada penelitian ini daun andong merah (Cordyline fruticosa (L.) A. Cheval) diekstraksi dengan menggunakan metode maserasi dengan pelarut etanol 70%. Hasil penelitian menunjukkan  antosianin jenis  sianidin. Aktivitas antioksidan diukur melalui peredaman radikal bebas DPPH dengan spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil Penelitian menunjukkan ekstrak daun andong merah   memiliki potensi  antioksidan  kuat dengan nilai IC50 64.5197 µg/mL terhadap radikal bebas  DPPH dan vitamin C sebagai pembanding menunjukkan potensi antioksidan sangat kuat dengan nilai  IC50 2.12 µg/mL.Kata Kunci : Daun andong merah, antioksidan, DPPH

Bioefficacy of Mikania micrantha H.B.K. against Water-Borne Bacterial Pathogens

2017 ◽  
Vol 5 (1) ◽  
pp. 52-57
Author(s):  
Awadhesh Kumar ◽  
◽  
Nagaraj Hegde ◽  
Keyword(s):  
Plant Secondary Metabolites ◽  
Mikania Micrantha ◽  
E Coli ◽  
Microdilution Method ◽  
Good Antibacterial Activity ◽  
Ic50 Value ◽  
Broth Microdilution Method ◽  
Thermo Stability ◽  
Microbial Attack

The plant secondary metabolites play a very important role during the microbial attack. These metabolites (essential oils/ alkaloids/ glycosides/ tannins etc.) can be extracted from plant and many of these plant products can be used as a potential source for new antibacterial agents. In thepresent investigation, the extract extracted from dried leaves of Mikania micrantha by using Soxlet apparatus. The efficacy of extract was analyzed by broth microdilution method of CLSI against the E. coli, K. pneumoniae, P. vulgaris and S. dysenteriae in in-vitro condition. The result showed the minimum inhibitory concentration 0.317mg/ ml against E. coli; 0.429mg/ ml against K. pneumoniae; 1.16mg/ml against P. vulgaris and 0.437mg/ ml against S. dysenteriae; however, IC50 value was recorded as 0.162, 0.211, 0.670 and 0.214, respectively. The finding showed that the extract of M. micrantha having a good antibacterial activity. Its toxicity persisted heavy inoculums density, thermo stability and long shelf life.

scholarly journals Synthesis and Characterization of Vanadium (IV)-Flavonoid Complexes and its Antioxidant ability toward Superoxide and Radical Scavenging

2020 ◽  
Author(s):  
Himyan Akbar ◽  
Salma Habib ◽  
Mohammed Mahroof Tahir ◽  
Lakshmaiah Sreerama
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Synthesis And Characterization ◽  
Vanadium Complex ◽  
Antioxidant Ability ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
Metal Charge Transfer

In this project Vanadium complex -Vanadium (IV) - flavone was synthesized using vanadium (IV) acetylacetonate (VO(acac)2) complex and 3-hydroxy-6-methyl flavone ligand. The complex stability was checked using FTIR and UV-vis spectroscopies. Peackes around 990 cm-1 conforms the formation of (V=O) in the complex, as well as (V-O) around 790 cm-1. In UV-Vis spectrum peak around 400-450 nm was noticed, which conforms the formation of the vanadium complex that correspond to the ligand to metal charge transfer (LMCT) transition. The radical scavenging abilities of vanadium complex were investigated using DPPH. The anti-oxidant activity using (BHA) as a standard reference, the complex synthesized displayed strong DPPH antioxidant radical scavenging activity compared to VO(acac)2 and BHA, with IC50 value of (105, 95 and 96) mM respectively. The absorbance in which the reducing power occurred were found to be (0.397, 0.825 and 0.228) for the complex, VO(acac)2 and BHA.

scholarly journals Anticancer activity of food grade nanoemulsions on hepatic cancer cells: a comparative study

2020 ◽  
Author(s):  
Sayyeda Hira Hassan ◽  
Muhammad Rehan Khan
Keyword(s):  
Olive Oil ◽  
Cell Line ◽  
Mtt Assay ◽  
Anticancer Agents ◽  
Cancer Cell Line ◽  
Flax Seed ◽  
Anticancer Activities ◽  
Ic50 Value ◽  
Flax Seeds

Abstract The current study was aimed to prepare stable NEs from plant oils to evaluate their cytotoxic effect on HepG2 cells. Oils form three different samples (flax seeds, cloves and olives) were extracted. NEs from these oils were prepared separately by ultrasonication method. After characterization of stable NEs, they were evaluated for their antioxidant and anticancer activities. Extracted oils and their NEs were applied separately to HepG2 cell line to construct a comparison and MTT assay was used for this purpose. Prepared NEs were found more effective against cancer cell line as compared to their respective oils. MTT assay showed efficacy of NEs in reducing cell viability with IC50 value of 10 µl (flax seed oil), 25 µl (clove oil NE) and 1 µl (olive oil NE), and was safe to normal cells. The results of the current study revealed that NEs prepared from flax seed, clove and olive oil have the potential for further development as anticancer agents.

scholarly journals Phytochemical screening, antibacterial and antioxidant assessment of Leuconotis eugenifolia leaf extract

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Praptiwi Praptiwi ◽  
DEWI WULANSARI ◽  
AHMAD FAHONI ◽  
NOTO HARNOTO ◽  
ROSSI NOVITA ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
E Coli ◽  
Microdilution Method ◽  
Ic50 Value ◽  
Dpph Scavenging

Abstract. Praptiwi, Wulansari D, Fahoni A, Harnoto N, Novita R, Alfridsyah, Agusta A. 2020. Phytochemical screening, antibacterial and antioxidant assessment of Leuconotis eugenifolia leaf extract. Nusantara Bioscience 12: 79-85. Traditionally, sitahe (Leuconotis eugenifolia (Wall. Ex G. Don) A.DC) has been used to maintain stamina by Aceh peoples, Indonesia. The use of L. eugenifolia mainly as a spice on a traditional cuisine “ie bu peudah”. This research was conducted to determine the phytoconstituents of L. eugenifolia leaf extracts, and to investigate their antioxidant potential using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and antibacterial activity against Staphylococcus aureus and Escherichia coli. Phytochemical screening was carried out using standard methods. L. eugenifolia leaves were extracted successively with n-hexane, dichloromethane, ethyl acetate, methanol, and water. Obtained extracts were tested qualitatively for antioxidant and antibacterial by the TLC-bioautography method, followed by quantitative analysis to determine the MIC and IC50 value using the microdilution method on 96-well microplate. The results showed that it contained alkaloids, flavonoids, steroids, triterpenoids, saponins, phenolics, and tannins. The highest levels of phenolic (259.0486 ± 0.1203 mg GAE/g extract) and flavonoids (84.6575 ± 0.0237 mg QE/g extract) were found in ethyl acetate extract, and dichloromethane, respectively. The antibacterial activity of n-hexane and dichloromethane extracts is moderate against E. coli, and weak against S. aureus. Ethyl acetate extract, methanol, and water extracts have very strong antioxidant activity. L. eugenifolia leaf extracts may be promising new antioxidant sources.

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