scholarly journals Aplikasi Hand Sanitizer Kitosan Cangkang Bekicot Sebagai Antibakteri dan Upaya Preventif Covid 19

SIMBIOSA ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 107
Author(s):  
Umarudin Umarudin ◽  
Surahmaida Surahmaida ◽  
Syukrianto Syukrianto ◽  
Silvi Ayu Wulansari ◽  
Siti Nurhaliza
Keyword(s):  
Klebsiella Pneumonia ◽  
Hand Sanitizer

Salah satu infeksi akibat bakteri yang dapat menyebabkan saluran pernapasan  adalah Klebsiella pneumonia. Desain yang digunnakan pada penelitian ini adalah true eksperimental. Tahapan penelitian dengan isolasi kitosan cangkang bekicot yaitu deproteinasi, demineralisasi, depigmentasi, dan deasetilasi, pembuatan hand sanitizer kitosan cangkang bekicot. Analisa data yang digunakan deskriptif kualitatif dan kuantatif untuk uji evaluasi organoleptis dan jumlah koloni bakteri dan uji ANOVA satu arah jika terdapat perbedaan dilakukan uji lanjut dengan uji LSD. Hasil penelitian menunjukan bahwa uji organoleptis hand sanitizer selama 28 hari dan didapatkan hasil sesuai spesifikasi yaitu berbentuk gel, warna transparan dan tidak ada aroma yang dikeluarkan, pH sediaan hand sanitizer sesuai dengan standart pH kulit, sediaan hand sanitizer kitosan cangkang bekicot homogen, jumlah koloni formula 2 (6%) dengan rata-rata 0,67 koloni, 3% dengan rata-rata 2,67 koloni lebih efektife alam menghambat pertumbuhan bakteri jika dibandingkan dengan K (-) dengan rata-rata 107,67. Perlakuan hand sanitizer 3% berbeda nyata secara signifikan dengan kontrol dan 6%, perlakuan hand sanitizer 6% berbeda nyata signifikan dengan kontrol dan 3%. Semakin tinggi konsentrasi cangkang bekicot semakin besar kemampuan daya hambat dan bersifat bakterisidal.

Fungicidal and Bactericidal Activity of the Alkyl-Substituted Guanidine-Containing Oligomers

2020 ◽  
Vol 82 (6) ◽  
pp. 54-63
Author(s):  
M.Ya. Vortman ◽  
◽  
Yu.B. Pysmenna ◽  
A.I. Chuenko ◽  
D.R. Abdulina ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Trichoderma Viride ◽  
Rhodococcus Erythropolis ◽  
Biological Action ◽  
Molar Ratio ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Alkyl Bromides ◽  
Base Form ◽  
Two Stages

Biocides are widely used in medicine and various industries to protect against a number of harmful microorganisms. Organic quaternary ammonium and guanidine-containing compounds, the biological action of which is based on membrane-toxic properties, are used as bactericidal preparations. The aim of this work was to study the bactericidal and fungicidal activities of the synthesized oligomeric alkylsubstituted guanidinium bromides with different radicals -C3H7, -C7H15, -C10H21, against different isolates of heterotrophic bacteria and microscopic fungi. Methods. The synthesis of alkyl-substituted guanidiniumcontaining oligomers was performed in two stages. In the first stage, alkyl-substituted guanidine was obtained by the reaction of guanidine, previously converted by alkali from the salt form to the base form by the base and alkyl bromides (Alk=-C3H7 (propyl), -C7H15 (heptyl), -C10H21 (decyl)) in methanol at a temperature of 50°C and a molar ratio of 1:1. The second carried out the reaction between aromatic oligoepoxide DER-331 and alkyl-substituted guanidine in methanol at a temperature of 50°C for 2–3 hours and a molar ratio of 1:2. Bacteria were grown on meat-peptone agar for 48 hours at a temperature of 28±2°С. Test cultures of micromycetes were cultured on agar beer wort (6°B), incubated for 14 days in a thermostat at a temperature of 28±2°C. Antimicrobial activity of newly synthesized alkyl-substituted guanidinium-containing oligomers was determined by standard disco-diffusion method (method of disks on agar) and fungicidal activity was determined by the method of holes in agar. Results. Oligomeric alkylsubstituted guanidinium bromides with different radicals composed -C3H7, -C7H15, -C10H21- synthesized by the reaction of guanidine alkyl bromides with aromatic oligoepoxydes. It was found that alkyl-substituted guanidinium-containing oligomers at a concentration of 1–3% inhibited the growth of Escherichia coli 475, Pseudomonas aeruginosa 465, Klebsiella pneumonia 479, Pseudomonas pseudoalcaligenes 109, Staphylococcus aureus 451, E. faecalis 422, Rhodococcus erythropolis 102, Bacillus subtilis 138 and most of the studied micromycetes – Aureobasidium pullulans F-41430, Paecilomyces variotii F-41432, Penicillium funiculosum F-41435, Penicillium ochrochloron F-41431, Scopulariopsis brevicaulis F-41434, Trichoderma viride F-41437, Candida albicans F-41441, Aspergillus flavus F-41442, Aspergillus niger F-41448, Penicillium sp. F-41447. Conclusions. Antimicrobial and fungicidal properties significantly depend on the length of the alkyl radical, with increasing of its length the diameter of the zone of bacterial and micromycetes growth retardation increases.10.15407/microbiolj82.06.054

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

High Alcohol-Producing Klebsiella Pneumonia is a Bacterial Culprit of Fatty Liver Disease

2019 ◽  
Author(s):  
Jing Yuan ◽  
Xiao Wei ◽  
Xiangna Zhao ◽  
Chen Chen ◽  
Jing Lu ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Klebsiella Pneumonia ◽  
High Alcohol

Public Awareness of Preventive Measures against COVID-19: A Google Trends Analysis (Preprint)

2020 ◽  
Author(s):  
Alex Mok ◽  
Oliver Oi Yat Mui ◽  
Kwan Pui Tang ◽  
Chi-Fai NG ◽  
Sunny Hei Wong ◽  
...  
Keyword(s):  
Public Health ◽  
Temporal Trend ◽  
Preventive Measures ◽  
Public Awareness ◽  
Google Trends ◽  
Online Searching ◽  
Effective Measure ◽  
Heat Maps ◽  
Hand Sanitizer ◽  
Incidence Data

BACKGROUND The 2019 coronavirus pandemic (COVID-19) has led to increase in global awareness of related public health preventive measures. The public awareness can be reflected by online searching trends of major search engines, namely Google Trends. OBJECTIVE This study aims to interpret online searches of COVID-19 related public health preventive measures and to identify possible correlations between early search trends and progression of the pandemic. METHODS Search data from five queries “Mask”, “Hand Washing”, “Social Distancing”, “Hand Sanitizer”, and “Disinfectant” were extracted from Google Trends (GT) in the form of Relative Search Volumes (RSV). Global incidence data of COVID-19 was obtained from January 1st to June 30th 2020. Subsequently, the data were analyzed and illustrated in forms of a global temporal RSV trend diagram, a geographical RSV distribution chart, scatter graphs comparing regional RSV with average daily cases; and heat-maps comparing temporal trend of RSV with average daily cases. RESULTS Global temporal trend revealed multiple surges in RSV, which were temporally associated with certain COVID news events. Geographical distribution showed differences of query interests among regions. Although scatter graphs failed to illustrate strong correlations between regional RSV and average daily cases, the heat-maps were able to demonstrate patterns of early RSV peaks in countries with lower average daily cases, for queries “Mask”, “Hand Sanitizer”, and “Disinfectant”, upon incorporating with the temporal element into analysis. CONCLUSIONS Early public awareness of multiple preventive measures was observed in countries with lower daily average cases. Public health authorities may look into early public awareness as an effective measure for future disease control.

Pemanfaatan Nira Aren Sebagai Bahan Baku Etanol Dalam Pembuatan Hand Sanitizer

2020 ◽  
Vol 1 (1) ◽  
pp. 13-23
Author(s):  
Adrianton Adrianton ◽  
M. Rusydi Hasanuddin ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Hand Sanitizer

Kebutuhan akan etanol (alkohol) dalam pembuatan cairan pencuci tangan (Hand Sanitizer) terus meningkat seirig wabah Virus Corona (Covid-19) yang belum juga mereda. Permintaan sangat tinggi sedangkan stok terbatas yang membuat masyarakat kesulitan untuk menemukan walaupun dipasaran ada tapi harganya cukup mahal, sehingga diperlukan bahan baku etanol alternatif yang dapat digunakan dan tersedia disekitar kita. Salah satu produk diversifikasi yang dapat dikembangkan adalah pohon aren yang menghasilkan etanol (alkohol) dari proses fermentasi (alami maupun buatan dengan tambahan ragi) dan destilasi. Metode yang digunakan dalam kegiatan pengabdian adalah ceramah/penyuluhan (koordinasi, sosialisasi dan penyadaran), praktik/pelatihan yang dilanjutkan dengan penerapan teknologi dan pendampingan tentang teknologi pengolahan nira aren secara terkendali menjadi Alkohol teknis dengan konsentrasi tinggi dan pembuatan Hand Sanitizer. Hasil Penyuluhan; yang telah dilaksanakan, di dapatkan data tentang 22 anggota mitra petani aren didominasi  laki-laki 90,91%, dengan tingkat pendidikan tertinggi SMP 36,36%, umur petani produktif antara 40 – 60 tahun sebesar 54,55%, lahan yang digunaan sebagai usaha adalah lahan milik sendiri dan kondisi tanaman dipelihara dengan baik 45,45%, produk yang dihasilkan 54,55% adalah gula merah dan 22,73% adalah produk Cap tikus/nira aren. Pada Praktik/Pelatihan; dilakukan pembuatan etanol/alkohol dari nira aren secara konvensional dengan kadar alkohol ≤25% (minuman cap tikus) sebanyak 20 Liter, kemudian dilakukan redestilasi untuk mendapatkan etanol teknis dengan kadar etanol di atas 80% sebanyak 9 Liter, lalu dibuat Hand Sanitizer bentuk Spray dan Gel sebanyak 100 botol. Proses Pendampingan; memberikan pengetahuan tentang teknik pemamfaatan nira aren untuk menghasilkan alkohol yang berkadar tinggi dengan teknik metode destilasi yang benar serta tingkat kebersihan produk untuk menjaga kualitasnya

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