scholarly journals Use of efficient microorganisms in agriculture

2021 ◽  
Vol 10 (8) ◽  
pp. e40610817515
Author(s):  
Giovani Mansani de Araujo Avila ◽  
Gislaine Gabardo ◽  
Djalma Cesar Clock ◽  
Osmair Silva de Lima Junior

The interest in the use of microorganisms in agricultural practices has increased significantly in recent years, both in the promotion of plant growth and in the biological control of plant pests and diseases. This literature review work aimed to address information on the use of isolation, multiplication, use and storage methodologies for efficient microorganisms (Effective Microorganisms, EM) in agriculture. These microorganisms have important functions for their hosts, as they have symbiotic interactions with them, and are capable of protecting plants from attack by insects, diseases and herbivorous mammals through the production of toxins. The use of EM in agriculture aims to accelerate the natural composition of organic matter and promote the balance of microbial flora contributing to plant development. EMs are potential substitutes for chemical products, and can thus favor the preservation of the environment. They are collected from fertile forest soils through simple and inexpensive methodologies, consisting of a tool with potential to be used both by family farmers and on a small and large scale. They can be used in different ways, the main ones being in soil, plant, water and animals. The use of EM is an accessible and low-cost technique, in addition to being easy to prepare within the property itself, contributing to the sustainability of agricultural systems.

Agronomy ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 219 ◽  
Author(s):  
Francisco Lagos Susaeta ◽  
Mario Maino ◽  
Lisette Lapierre ◽  
Pilar Oviedo ◽  
Ricardo Riquelme ◽  
...  

Improvements and good practices (GPs) in rural environments are often developed by peasants themselves and shared among trust-based networks. The level of adoption of GPs by peasant family farmers (PFF) has been poorly studied. This paper describes the performance and results of the innovation adoption index (InAI) and rate (InAR) which were used to estimate the adoption of GPs for pesticide and veterinary drug use by PFF from eight different regions of Chile. Surveys were conducted among 257 farmers to find out about the adoption (yes/no) of a set of GPs in the adequate handling, use and storage of these chemical products, as well as some identifying information. The farmers in this study are producers of berries, dairy cows, honey and vegetables. The results of the survey showed an average of 57.33% GPs were adopted by farmers. Group averages of 55.23–61.32% were observed in the numbers of practices adopted by farmers. This survey data collection was part of a wider study intended to design a national plan to reduce chemical residues in food produced by PFF in Chile, with a focus on organizing practical workshops with extension officers and farmers.


Nanoscale ◽  
2016 ◽  
Vol 8 (21) ◽  
pp. 11291-11305 ◽  
Author(s):  
Manickam Minakshi ◽  
David Mitchell ◽  
Rob Jones ◽  
Feraih Alenazey ◽  
Teeraphat Watcharatharapong ◽  
...  

Electrochemical energy production and storage at large scale and low cost, is a critical bottleneck in renewable energy systems.


2010 ◽  
Vol 10 (1) ◽  
pp. 80-88
Author(s):  
IGRM Temaja ◽  
G. Suastika ◽  
S.H. Hidayat ◽  
U. Kartosuwondo

Antiserum production and serological assay of Chrysanthemum B Carlavirus (CVB). Virus identification based on spesific reaction between antigen and antibody  in serological assay has been widely applied as a tool for plant virus detection. The aims of this research is  to produce  antiserum of the CVB by  guinea pig immunization using  purified CVB of Cianjur isolate. The antiserum   was used further  for  the  serological test. Serological methods for detection of CVB were I-ELISA, TBIA, western blot and ISEM. The result showed that  guinea pig immunization  using 150 µg of purified virus was able to produce 10.75 ml of antiserum. The antiserum produced had high sensitivity for detection of CVB when examined by I-ELISA and TBIA. Besides its low cost, TBIA allows the samples to be blotted on the nitrocellulose membranes in the field and storage of the membranes for later processing in the laboratory. This feature makes it the metode of  choice for large-scale CVB surveying.


Sensors ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 2079 ◽  
Author(s):  
Longlong Liao ◽  
Kenli Li ◽  
Canqun Yang ◽  
Jie Liu

When measurement rates grow, most Compressive Sensing (CS) methods suffer from an increase in overheads of transmission and storage of CS measurements, while reconstruction quality degrades appreciably when measurement rates reduce. To solve these problems in real scenarios such as large-scale distributed surveillance systems, we propose a low-cost image CS approach called MRCS for object detection. It predicts key objects using the proposed MYOLO3 detector, and then samples the regions of the key objects as well as other regions using multiple measurement rates to reduce the size of sampled CS measurements. It also stores and transmits half-precision CS measurements to further reduce the required transmission bandwidth and storage space. Comprehensive evaluations demonstrate that MYOLO3 is a smaller and improved object detector for resource-limited hardware devices such as surveillance cameras and aerial drones. They also suggest that MRCS significantly reduces the required transmission bandwidth and storage space by declining the size of CS measurements, e.g., mean Compression Ratios (mCR) achieves 1.43–22.92 on the VOC-pbc dataset. Notably, MRCS further reduces the size of CS measurements by half-precision representations. Subsequently, the required transmission bandwidth and storage space are reduced by one half as compared to the counterparts represented with single-precision floats. Moreover, it also substantially enhances the usability of object detection on reconstructed images with half-precision CS measurements and multiple measurement rates as compared to its counterpart, using a single low measurement rate.


2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Liuzhang Ouyang ◽  
Jun Jiang ◽  
Kang Chen ◽  
Min Zhu ◽  
Zongwen Liu

AbstractAs an environmentally friendly and high-density energy carrier, hydrogen has been recognized as one of the ideal alternatives for fossil fuels. One of the major challenges faced by “hydrogen economy” is the development of efficient, low-cost, safe and selective hydrogen generation from chemical storage materials. In this review, we summarize the recent advances in hydrogen production via hydrolysis and alcoholysis of light-metal-based materials, such as borohydrides, Mg-based and Al-based materials, and the highly efficient regeneration of borohydrides. Unfortunately, most of these hydrolysable materials are still plagued by sluggish kinetics and low hydrogen yield. While a number of strategies including catalysis, alloying, solution modification, and ball milling have been developed to overcome these drawbacks, the high costs required for the “one-pass” utilization of hydrolysis/alcoholysis systems have ultimately made these techniques almost impossible for practical large-scale applications. Therefore, it is imperative to develop low-cost material systems based on abundant resources and effective recycling technologies of spent fuels for efficient transport, production and storage of hydrogen in a fuel cell-based hydrogen economy.


2020 ◽  
pp. 102-109
Author(s):  
D.KH. DOMULLODZHANOV ◽  
◽  
R. RAHMATILLOEV

The article presents the results of the field studies and observations that carried out on the territory of the hilly, low-mountain and foothill agro landscapes of the Kyzylsu-yuzhnaya (Kyzylsu-Southern) River Basin of Tajikistan. Taking into account the high-altitude location of households and the amount of precipitation in the river basin, the annual volumes of water accumulated with the use of low-cost systems of collection and storage of precipitation have been clarified. The amount of water accumulated in the precipitation collection and storage systems has been established, the volume of water used for communal and domestic needs,the watering of livestock and the amount of water that can be used to irrigate crops in the have been determined. Possible areas of irrigation of household plots depending on the different availability of precipitation have been determined. It has been established that in wet years (with precipitation of about 10%) the amount of water collected using drip irrigation will be sufficient for irrigation of 0.13 hectares, and in dry years (with 90% of precipitation) it will be possible to irrigate only 0.03 ha of the household plot. On the basis of the basin, the total area of irrigation in wet years can be 4497 ha, and in dry years only 1087 ha. Taking into account the forecasts of population growth by 2030 and an increase in the number of households, the total area of irrigation of farmlands in wet years may reach 5703 hectares,and in dry years – 1379 hectares. Growing crops on household plots under irrigation contributes to a significant increase in land productivity and increases the efficiency of water use of the Kyzylsu-yuzhnaya basin.


1987 ◽  
Vol 19 (5-6) ◽  
pp. 701-710 ◽  
Author(s):  
B. L. Reidy ◽  
G. W. Samson

A low-cost wastewater disposal system was commissioned in 1959 to treat domestic and industrial wastewaters generated in the Latrobe River valley in the province of Gippsland, within the State of Victoria, Australia (Figure 1). The Latrobe Valley is the centre for large-scale generation of electricity and for the production of pulp and paper. In addition other industries have utilized the brown coal resource of the region e.g. gasification process and char production. Consequently, industrial wastewaters have been dominant in the disposal system for the past twenty-five years. The mixed industrial-domestic wastewaters were to be transported some eighty kilometres to be treated and disposed of by irrigation to land. Several important lessons have been learnt during twenty-five years of operating this system. Firstly the composition of the mixed waste stream has varied significantly with the passage of time and the development of the industrial base in the Valley, so that what was appropriate treatment in 1959 is not necessarily acceptable in 1985. Secondly the magnitude of adverse environmental impacts engendered by this low-cost disposal procedure was not imagined when the proposal was implemented. As a consequence, clean-up procedures which could remedy the adverse effects of twenty-five years of impact are likely to be costly. The question then may be asked - when the total costs including rehabilitation are considered, is there really a low-cost solution for environmentally safe disposal of complex wastewater streams?


1996 ◽  
Vol 33 (4-5) ◽  
pp. 39-44
Author(s):  
J. Holas ◽  
M. Konvicková

Potential environmental impacts as a result of large-scale farming system in the Czech Republic have created a great deal of concern in recent years. This concern has led to several studies to identify the role of new regulations, directives and other legislative issues in the field of water pollution control. The set of legislative tools related to watershed management policy to promote better agricultural practices is shortly reviewed. The paper emphasises the running water law system amendment with respect to European community water quality regulations.


BMC Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Amrita Srivathsan ◽  
Emily Hartop ◽  
Jayanthi Puniamoorthy ◽  
Wan Ting Lee ◽  
Sujatha Narayanan Kutty ◽  
...  

Abstract Background More than 80% of all animal species remain unknown to science. Most of these species live in the tropics and belong to animal taxa that combine small body size with high specimen abundance and large species richness. For such clades, using morphology for species discovery is slow because large numbers of specimens must be sorted based on detailed microscopic investigations. Fortunately, species discovery could be greatly accelerated if DNA sequences could be used for sorting specimens to species. Morphological verification of such “molecular operational taxonomic units” (mOTUs) could then be based on dissection of a small subset of specimens. However, this approach requires cost-effective and low-tech DNA barcoding techniques because well-equipped, well-funded molecular laboratories are not readily available in many biodiverse countries. Results We here document how MinION sequencing can be used for large-scale species discovery in a specimen- and species-rich taxon like the hyperdiverse fly family Phoridae (Diptera). We sequenced 7059 specimens collected in a single Malaise trap in Kibale National Park, Uganda, over the short period of 8 weeks. We discovered > 650 species which exceeds the number of phorid species currently described for the entire Afrotropical region. The barcodes were obtained using an improved low-cost MinION pipeline that increased the barcoding capacity sevenfold from 500 to 3500 barcodes per flowcell. This was achieved by adopting 1D sequencing, resequencing weak amplicons on a used flowcell, and improving demultiplexing. Comparison with Illumina data revealed that the MinION barcodes were very accurate (99.99% accuracy, 0.46% Ns) and thus yielded very similar species units (match ratio 0.991). Morphological examination of 100 mOTUs also confirmed good congruence with morphology (93% of mOTUs; > 99% of specimens) and revealed that 90% of the putative species belong to the neglected, megadiverse genus Megaselia. We demonstrate for one Megaselia species how the molecular data can guide the description of a new species (Megaselia sepsioides sp. nov.). Conclusions We document that one field site in Africa can be home to an estimated 1000 species of phorids and speculate that the Afrotropical diversity could exceed 200,000 species. We furthermore conclude that low-cost MinION sequencers are very suitable for reliable, rapid, and large-scale species discovery in hyperdiverse taxa. MinION sequencing could quickly reveal the extent of the unknown diversity and is especially suitable for biodiverse countries with limited access to capital-intensive sequencing facilities.


Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 60
Author(s):  
Vincenzo Michele Sellitto ◽  
Severino Zara ◽  
Fabio Fracchetti ◽  
Vittorio Capozzi ◽  
Tiziana Nardi

From a ‘farm to fork’ perspective, there are several phases in the production chain of fruits and vegetables in which undesired microbial contaminations can attack foodstuff. In managing these diseases, harvest is a crucial point for shifting the intervention criteria. While in preharvest, pest management consists of tailored agricultural practices, in postharvest, the contaminations are treated using specific (bio)technological approaches (physical, chemical, biological). Some issues connect the ‘pre’ and ‘post’, aligning some problems and possible solution. The colonisation of undesired microorganisms in preharvest can affect the postharvest quality, influencing crop production, yield and storage. Postharvest practices can ‘amplify’ the contamination, favouring microbial spread and provoking injures of the product, which can sustain microbial growth. In this context, microbial biocontrol is a biological strategy receiving increasing interest as sustainable innovation. Microbial-based biotools can find application both to control plant diseases and to reduce contaminations on the product, and therefore, can be considered biocontrol solutions in preharvest or in postharvest. Numerous microbial antagonists (fungi, yeasts and bacteria) can be used in the field and during storage, as reported by laboratory and industrial-scale studies. This review aims to examine the main microbial-based tools potentially representing sustainable bioprotective biotechnologies, focusing on the biotools that overtake the boundaries between pre- and postharvest applications protecting quality against microbial decay.


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