scholarly journals Determination of 96 h LC50 Value and Acute Toxicity of Marshal (Carbosulfan 25%EC) on Thyroid and Reproductive Hormones of Labeo rohita (Hamilton)

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dey Chandrima ◽  
Saha Samir Kumar
Keyword(s):  
Labeo Rohita ◽  
Probit Analysis ◽  
Reproductive Hormones ◽  
Mortality Data ◽  
Indian Major Carp ◽  
Lc50 Value ◽  
Serum T3 ◽  
Fish Productivity ◽  
Carbamate Pesticide

Contamination of water bodies by pesticides can lead to fish death and reduced fish productivity etc. which can affect humans consuming these fishes as a source of protein. The present study was designed to determine the 96h LC50 value of a carbamate pesticide, Marshal (Carbosulfan 25%EC) and to investigate effects of this pesticide on the thyroid and reproductive hormones profile of Indian major carp Labeo rohita (Hamilton). The present study indicates that Marshal has potential hormonal stress on Labeo rohita. The mortality data obtained were analyzed by SPSS 16.0 based on Finney’s Probit Analysis Statistical method. The 96h LC50 value for Marshal was found to be 10 ?l/L. Effects of this carbamate pesticide on serum T3, T4 and TSH, testosterone and estradiol were investigated at sub-lethal concentration. Serum T3, T4 and TSH were studied for 96 h and testosterone and estradiol for 4 days and 15 days. These parameters (except TSH) decrease after exposure of the fish to pesticide.

scholarly journals Acute and sublethal intoxication of malathion in an Indian major carp, Labeo rohita: haematological and biochemical responses.

2021 ◽  
Vol 36 (3) ◽  
pp. e2021016
Author(s):  
Juginu Mankuzhiyil Sivanandan ◽  
Binukumari
Keyword(s):  
Sublethal Effects ◽  
Labeo Rohita ◽  
Hematological Parameters ◽  
Organophosphorus Pesticide ◽  
Control Group ◽  
Indian Major Carp ◽  
Biochemical Responses ◽  
Lc50 Value ◽  
Hematological And Biochemical Parameters ◽  
The Impact

This study aimed to determine the median lethal concentration (96-h LC50), acute and sublethal effects of malathion, an organophosphorus pesticide on hematological and biochemical responses in an Indian major carp, Labeo rohita. In this study, the LC50 value of malathion for 96 h was found to be 3.4 ppm. During acute (3.4 ppm) and sublethal [1/10th of 96 h LC50 value (0.34 ppm) studies, all the hematological parameters except WBC were significantly decreased (p<0.05). Besides, when compared with the control group, a significant (p<0.05) decrease in biochemical activity was also observed in malathion treated fish during acute and sublethal exposure periods. These results suggest that the tested concentrations of malathion could have significant adverse effects on the hematological and biochemical parameters of fish, Labeo rohita. The changes in the parameters can be effectively used to determine the impact of malathion in the aquatic ecosystem.

scholarly journals Acute toxicity of metal cyanides to Indian major carp Labeo rohita (Hamilton)

2010 ◽  
Vol 26 (3-4) ◽  
pp. 267-277
Author(s):  
B.B. Hosetti ◽  
P.N. Dube ◽  
M.S. Prashanth ◽  
A. Shwetha
Keyword(s):  
Acute Toxicity ◽  
Labeo Rohita ◽  
Test Chamber ◽  
Sodium Cyanide ◽  
Indian Major Carp ◽  
Vertical Movements ◽  
Metal Cyanide ◽  
Lc50 Value ◽  
Metal Cyanides ◽  
Cyanide Complexes

Static renewal bioassay tests were carried out to determine the acute toxicity (LC50) of metal cyanides to the Indian major carp Labeo rohita. The 96 hour LC50 value for the sodium cyanide, zinc cyanide and copper cyanide to the fish L. rohita were 0.32 mg/L 0.35 mg/L, and 1.1 mg/L respectively. Among the metal cyanide tested, sodium cyanide is found to be more toxic than the other cyanide complexes. In general behavioral responses of the fishes exposed to cyanide included uncontrolled swimming, erratic movements, loss of balance, moving spiral fashion with sudden jerky movements, vertical movements lying on the sides of the test chamber and rapid flapping of the opercular movements with opened mouth finally settles to the bottom.

scholarly journals Uji Toksisitas Akut Logam Timbal (Pb), Krom (Cr) dan Kobalt (Co) terhadap Daphnia Magna

Jurnal Dampak ◽  
2017 ◽  
Vol 14 (1) ◽  
pp. 33 ◽  
Author(s):  
Tivany Edwin ◽  
Taufiq Ihsan ◽  
Windy Pratiwi
Keyword(s):  
Daphnia Magna ◽  
Preliminary Test ◽  
Quality Standards ◽  
Probit Analysis ◽  
Mortality Data ◽  
Analysis Program ◽  
Metallic Lead ◽  
Static Test ◽  
Lc50 Value ◽  
Program Version

This study aimed to analyze the LC50 value of metallic lead (Pb), chromium (Cr), and cobalt (Co) contained in the printing industry wastewater to Daphnia magna and analyze the relationship of these metals to the LC50 value. The measurement results konsnetrasi Pb and Cr do not exceed the quality standards established, but the metals Co exceeds quality standards. Toxicity of Pb, Cr, and Co were tested in static test using artificial solution of Pb, Cr, and Co in accordance with the concentration measured in the wastewater. Tests carried out consisted of two stages: a preliminary test and test base. Daphnia magna mortality data were analyzed using Probit method with the EPA program Probit Analysis Program Version 1.5. 24-hour LC50 value of Pb, Cr, and Co to Daphnia magna is 1.052% and the LC50 values of Pb, Cr, and Co are respectively 0.003 mg / l, 0.008 mg / l and 0.009 mg / l. All three metals have included the category of very toxic to Dahnia magna.Keywords: Pb, Cr, Co, Daphnia magna, LC50Abstrak-Penelitian ini bertujuan untuk menganalisis nilai LC50 logam timbal (Pb), Krom (Cr), dan Kobalt (Co) yang terdapat pada limbah cair industri percetakan terhadap Daphnia magna serta menganalisis hubungan logam tersebut terhadap nilai LC50. Hasil pengukuran konsnetrasi logam Pb dan Cr tidak melebihi baku mutu yang ditetapkan, namun logam Co melebihi baku mutu. Toksisitas logam Pb, Cr, dan Co diuji dalam static test menggunakan larutan artifisial logam Pb, Cr, dan Co sesuai dengan konsentrasi terukur pada limbah cair. Pengujian dilakukan terdiri dari 2 tahap yaitu uji pendahuluan dan uji dasar. Data kematian Daphnia magna dianalisis menggunakan Metode Probit dengan program EPA Probit Analysis Program Version 1.5. Nilai LC50 24 jam logam Pb, Cr, dan Co terhadap Daphnia magna adalah 1,052% dan nilai LC50 logam Pb, Cr, dan Co berturut-turut adalah 0,003 mg/l, 0,008 mg/l, dan 0,009 mg/l. Ketiga logam ini termasuk kategori sangat toksik terhadap Dahnia magna.Kata Kunci: Pb, Cr, Co, Daphnia magna, LC50

scholarly journals Aktivitas larvasida fraksi metanol dan etil asetat buah lakum (Cayratia trifolia (L.) Domin.) terhadap larva nyamuk Aedes aegypti

2021 ◽  
Vol 10 (1) ◽  
pp. 1-13
Author(s):  
Diah Wulandari Rousdy ◽  
Elvi Rusmiyanto Pancaning Wardoyo ◽  
Siti Ifadatin
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Hemorrhagic Fever ◽  
Ethyl Acetate Fraction ◽  
Probit Analysis ◽  
Mortality Data ◽  
Phytochemical Analysis ◽  
Methanol Fraction ◽  
Lc50 Value ◽  
Cayratia Trifolia

ABSTRAKAedes aegypti merupakan serangga vektor dari virus Dengue penyebab Demam Berdarah Dengue (DBD). Kandungan senyawa dalam tumbuhan lakum berpotensi sebagai larvasida. Buah lakum dimaserasi dengan pelarut methanol kemudian difraksinasi menggunakan etil asetat. Fraksi diteliti kandungan senyawanya menggunakan analisis fitokimia. Uji larvasida diamati selama 48 jam terdiri dari tujuh konsentrasi berseri yaitu kontrol; 0,04; 0,12; 0,2; 0,6; 1; 3% (b/v). Setiap konsentrasi diulang 3 kali dan setiap ulangan terdiri dari 10 ekor larva nyamuk Ae. aegypti. Data mortalitas dianalisis menggunakan analisis probit untuk menentukan nilai LC50. Fraksi metanol buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,125%. Fraksi etil asetat  buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,102%. Buah lakum mengandung senyawa: polifenol, kuinon, flavonoid, alkaloid dan terpenoid. Kata kunci: Aedes aegypti; Cayratia trifolia (L.) Domin; larvasida; LC50  ABSTRACTLarvacidal activity of methanol and ethyl acetate fraction of lakum fruits (Cayratia trifolia (L.) Domin.) against Aedes aegypti larvae. Aedes aegypti is a vector from the Dengue virus which causes Dengue Hemorrhagic Fever (DHF). The compounds of lakum plants has the potential larvicide. The fruit of C. trifolia was macerated with methanol then fractionated using ethyl acetate as a solvent. The fraction was examined for its compound using phytochemical analysis. Larvacide test was observed for 48 hours consisting of seven concentration of 0 (control); 0.04; 0.12; 0.2; 0.6; 1; 3% (w/v). Each concentration was repeated 3 times and each repetition consisted of 10 larvae of Ae. aegypti. Mortality data were analyzed using probit analysis to determine the LC50 of the fraction of methanol and ethyl acetate from lakum fruit. The methanol fraction has the potential larvicide with a six hour LC50 value 1.125%. The ethyl acetate fraction has the potential larvicide with a six hour LC50 value 1.102%. The C. trifolia fruits contains polyphenols, quinones, flavonoids, alkaloids and terpenoids. Keywords: Aedes aegypti; Cayratia trifolia (L.) Domin.; larvacide, LC50

scholarly journals Effects of Dimethoate Insecticide on Serum Biochemistry of African Catfish (Clarias gariepinus) Juveniles

2021 ◽  
Vol 25 (6) ◽  
pp. 977-985
Author(s):  
O.O. Oghale ◽  
A.C. Okoboshi ◽  
A.P. Chinedu-Ndukwe ◽  
K.C. Kanu ◽  
M. Beka ◽  
...  
Keyword(s):  
Clarias Gariepinus ◽  
Exposure Period ◽  
Serum Biochemistry ◽  
Probit Analysis ◽  
Mortality Data ◽  
African Catfish ◽  
Exposure Concentration ◽  
Lc50 Value ◽  
Run Off ◽  
Static Bioassay

The present study was designed to investigate acute toxicity of  dimethoate on juvenile Clarias gariepinus. Catfish with mean weight and length of 24.01 ± 11.60 g and 13.98 ± 5.66 cm respectively were exposed to sub-lethal levels of dimethoate in static bioassay system for 28 days and mortality data was statistically evaluated using Finney’s Probit analysis. The 96-hour LC50 value for C. gariepinus was estimated as 29.05 mg/l. The value for the LC50 was used in deducing the sub-lethal concentrations (0.01, 0.15and 0.29 mg/l). Biochemical changes in the serum such as glycogen, total protein, cholesterol, urea and creatinine were analyzed after the exposure period (28 days). The glycogenlevel decreased in all sublethal exposures. An increase in cholesterol at lower exposure concentration, followed by slight increase in urea and creatinine among treated groups was recorded. Behavioural changes which includes changes in skin colour, jerking, restlessness, erratic swimming, among others were observed. Based on the result of this study, it was concluded that C. gariepinus juveniles are very sensitive to low concentration of dimethoate. Dimethoate is highly toxic to juvenile catfishes and as such measures must be taken to avoid its run-off into our aquatic environment thus jeopardizing the recruitment of juvenile catfishes into the fishery.

scholarly journals Resistance Monitoring of Nilaparvata lugens Stall against Pymetrozine Insecticide with Determination of Diagnostic Concentrations

2021 ◽  
Vol 13 (1) ◽  
pp. 58-64
Author(s):  
Sri Murtiati ◽  
Udi Tarwotjo ◽  
Rully Rahadian
Keyword(s):  
Insect Pests ◽  
Nilaparvata Lugens ◽  
Probit Analysis ◽  
Concentration Limit ◽  
Lc50 Value ◽  
Central Java ◽  
Bioassay Test ◽  
Sensitive Population ◽  
Intensive Control

Nilaparvata lugens Stall is one of the main insect pests on rice crops. Intensive control of this pests using insecticides has resulted in the development of insect resistance. This study aimed to find out the level of resistance of the N. lugens population to pymetrozine insecticides by determining the diagnostic concertations. N. lugens was collected from five endemic areas in Central Java Province from October 2019 to June 2020. The data from the bioassay test were analyzed with probit analysis to obtain the LC50 value. The results of the sensitivity test showed that the Kajen population has the highest RF value (2.47), while the Karanganyar population which has the lowest RF value (1) was the most sensitive population. The determined diagnostic concentration was LC95 = 25.52 ppm with the lowest concentration limit of 7.67 ppm and the highest of 30.05 ppm. To conclude, the determined diagnostic concentration (LC95 = 25.52 ppm) is effective for detecting the susceptibililty of N. lugens population. This finding would be beneficial for monitoring resistance of N. lugens population against pymetrozine insecticides in the field.

Increased water temperature interrupts embryonic and larval development of Indian major carp rohu Labeo rohita

2021 ◽  
Author(s):  
Mohammad Ashaf-Ud-Doulah ◽  
S. M. Majharul Islam ◽  
Md Mahiuddin Zahangir ◽  
Md Sadiqul Islam ◽  
Christopher Brown ◽  
...  
Keyword(s):  
Water Temperature ◽  
Larval Development ◽  
Labeo Rohita ◽  
Indian Major Carp ◽  
Embryonic And Larval Development

scholarly journals Determination of lethal concentration and antibacterial activity of commonly used disinfectants

1970 ◽  
Vol 1 (4) ◽  
pp. 102-105 ◽  
Author(s):  
M Alam ◽  
MM Rahman ◽  
MJ Foysal ◽  
MN Hossain
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Fluorescens ◽  
Potassium Permanganate ◽  
Malachite Green ◽  
Copper Sulfate ◽  
Pathogenic Bacteria ◽  
Labeo Rohita ◽  
Inhibitory Effect ◽  
Lethal Concentration

The toxic effects of four disinfectants viz., copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green on fish and fish pathogenic bacteria Aeromonas sp., Pseudomonas fluorescens, Edwardsiella sp. and Flavobacterium sp. were investigated. Lethal concentration of the disinfectants to fingerlings of Labeo rohita was determined in aquarium by standard method. Lethal concentration of copper sulfate (CuSO4), potassium permanganate (KMnO4), methylene blue and malachite green against fish were found in 0.75ppm, 7ppm, 6ppm and 0.5ppm at 21.4hrs, 18hrs, 9.5hrs and 1.40hrs, respectively. Methylene blue at 4ppm and 5ppm concentration inhibited the growth of Pseudomonas fluorescens and 6ppm concentration suppressed the growth of Aeromonas sp. Copper sulfate (CuSO4) was effective only against Edwardsiella sp at concentration of 10ppm and 8ppm. Malachite green repressed the growth of all four tasted bacteria at a concentration of 1ppm. Potassium permanganate (KMnO4) was failed to exhibit any inhibitory effect on the bacteria even at 30ppm concentration. DOI: http://dx.doi.org/10.3329/ijns.v1i4.9738 IJNS 2011 1(4): 102-105

Efficacy of two avermectins, doramectin and ivermectin against Argulus siamensis infestation in Indian major carp, Labeo rohita

2012 ◽  
Vol 190 (1-2) ◽  
pp. 297-304 ◽  
Author(s):  
K.P. Hemaprasanth ◽  
Banya Kar ◽  
S.K. Garnayak ◽  
J. Mohanty ◽  
J.K. Jena ◽  
...  
Keyword(s):  
Labeo Rohita ◽  
Indian Major Carp ◽  
Argulus Siamensis
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