scholarly journals Aktivitas larvasida fraksi metanol dan etil asetat buah lakum (Cayratia trifolia (L.) Domin.) terhadap larva nyamuk Aedes aegypti

2021 ◽  
Vol 10 (1) ◽  
pp. 1-13
Author(s):  
Diah Wulandari Rousdy ◽  
Elvi Rusmiyanto Pancaning Wardoyo ◽  
Siti Ifadatin
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Hemorrhagic Fever ◽  
Ethyl Acetate Fraction ◽  
Probit Analysis ◽  
Mortality Data ◽  
Phytochemical Analysis ◽  
Methanol Fraction ◽  
Lc50 Value ◽  
Cayratia Trifolia

ABSTRAKAedes aegypti merupakan serangga vektor dari virus Dengue penyebab Demam Berdarah Dengue (DBD). Kandungan senyawa dalam tumbuhan lakum berpotensi sebagai larvasida. Buah lakum dimaserasi dengan pelarut methanol kemudian difraksinasi menggunakan etil asetat. Fraksi diteliti kandungan senyawanya menggunakan analisis fitokimia. Uji larvasida diamati selama 48 jam terdiri dari tujuh konsentrasi berseri yaitu kontrol; 0,04; 0,12; 0,2; 0,6; 1; 3% (b/v). Setiap konsentrasi diulang 3 kali dan setiap ulangan terdiri dari 10 ekor larva nyamuk Ae. aegypti. Data mortalitas dianalisis menggunakan analisis probit untuk menentukan nilai LC50. Fraksi metanol buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,125%. Fraksi etil asetat  buah lakum  berpotensi sebagai larvasida dengan nilai LC50 6 jam sebesar 1,102%. Buah lakum mengandung senyawa: polifenol, kuinon, flavonoid, alkaloid dan terpenoid. Kata kunci: Aedes aegypti; Cayratia trifolia (L.) Domin; larvasida; LC50  ABSTRACTLarvacidal activity of methanol and ethyl acetate fraction of lakum fruits (Cayratia trifolia (L.) Domin.) against Aedes aegypti larvae. Aedes aegypti is a vector from the Dengue virus which causes Dengue Hemorrhagic Fever (DHF). The compounds of lakum plants has the potential larvicide. The fruit of C. trifolia was macerated with methanol then fractionated using ethyl acetate as a solvent. The fraction was examined for its compound using phytochemical analysis. Larvacide test was observed for 48 hours consisting of seven concentration of 0 (control); 0.04; 0.12; 0.2; 0.6; 1; 3% (w/v). Each concentration was repeated 3 times and each repetition consisted of 10 larvae of Ae. aegypti. Mortality data were analyzed using probit analysis to determine the LC50 of the fraction of methanol and ethyl acetate from lakum fruit. The methanol fraction has the potential larvicide with a six hour LC50 value 1.125%. The ethyl acetate fraction has the potential larvicide with a six hour LC50 value 1.102%. The C. trifolia fruits contains polyphenols, quinones, flavonoids, alkaloids and terpenoids. Keywords: Aedes aegypti; Cayratia trifolia (L.) Domin.; larvacide, LC50

scholarly journals Skrining Fitokimia dan Toksisitas dari Ekstrak Daun Pecut Kuda (Stachytarpheta jamaicenesis (L.) Vahl)

2021 ◽  
Vol 5 (1) ◽  
pp. 28
Author(s):  
Muhammad Wildan Rizqi Miftahussanadi ◽  
Erwin Erwin ◽  
Irawan Wijaya Kusuma
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Hexane Fraction ◽  
Toxicity Tests ◽  
Methanol Fraction ◽  
Tropical Regions ◽  
Lc50 Value ◽  
East Kalimantan

The Pecut kuda plant is one of the plants of the genus Stachytarpheta which is found in tropical regions, including Indonesia. In Indonesia this plant is found in East Kalimantan which grows wild. This study aims to determine the type of secondary metabolites and the level of toxicity of the leaves of horse whip (Stachytarpheta jamaicenesis (L.) Vahl). The research stages were sample drying, sample maceration, filtered, concentrated with a rotary evaporator, phytochemical and toxicity tests using linear regression values to determine the LC50 value with SAS probit analysis. Phytochemical test results showed that the pecut horse leaf extract in the n-hexane fraction contained flavonoids, quinones and steroids, the ethyl acetate fraction contained alkaloids, steroids, saponins, flavonoids and quinones and the methanol fraction contained phenolic flavonoids, quinones and saponins. The results of the toxicity test on Artemia salina L. shrimp larvae in the fraction of n-hexane, ethyl acetate and methanol obtained LC50 values of 0.036 ppm, 5.24 ppm and 29.51 ppm, respectively. Based on the LC50 value, it can be concluded that the three fractions are very toxic, but the most toxic is the n-hexane fraction (LC50 = 0.036 ppm).

scholarly journals Phytochemical, antimicrobial, antioxidant and enzyme inhibitory potential of medicinal plant Dryopteris ramosa (Hope) C. Chr.

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fiaz Alam ◽  
Syed Hurmat Ali Khan ◽  
Mohammad Hassham Hassan Bin Asad
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Methanol Fraction ◽  
Medicinal Uses ◽  
Antibiotic Effect ◽  
Esterase Inhibition ◽  
Mic Value

Abstract Background Dryopteris ramosa has numerous potentials uses in the treatment of different maladies as old traditional medication. The fronds of D. ramose are edible and orally administered for producing antibiotic effect. They are also used as astringent and febrifuge, and as a pesticide. Methods Extraction of fronds of D. ramosa using solvents of increasing polarity, namely, ethyl acetate, methanol and water were tested for phytochemical (qualitative tests, GC-MS), antimicrobial (well method), antioxidant (DPPH), antifungal (tube dilution), cytotoxic activity (brine shrimps lethality assay) and LOX and COX inhibitory activities were performed using standard methods. Results The phytochemical analysis of the crude methanolic extract revealed that the fronds are rich in flavonoids, alkaloids, saponins, tannins, glycosides and triterpenoids. The total flavonoid content of the ethyl acetate fraction was 46.28 μg QE/mg extract. The GC-MS analysis revealed nine major compounds that constituted the crude drug and potentially had a role in reported activities. The crude extract was the most active amongst all the fractions against the bacterial and fungal strains used such that it inhibited the growth of P. aeruginosa with a zone of 13 mm and a MIC value of 16 μg/ml as compared to the standard cefixime, which inhibited the zone by only 10 mm and a MIC value of 32 μg/ml. The highest antioxidant potential in DPPH assay was shown by the crude extract with 91.948% free radical scavenging activity. The bring shrimps lethality potential of the crude extract was the highest, with a LD50 value of 47.635 μg/ml. The ethyl acetate fraction inhibits 91.36% of alpha glucosidase enzyme at a concentration of 0.5 mg/ml. In case of acetylcholine esterase inhibition assay, the methanol fraction inhibits 58.26% of the enzyme activity. Similarly, for butyrylcholine esterase inhibition, the maximum inhibitory effect was seen in the methanol fraction, with a percentage inhibition of 47.32%. Conclusion These test results support traditional medicinal uses of the plant. Dryopteris ramosa could be imperative for being used as a therapeutic agent and the medicinal importance of this plant should be further investigated.

scholarly journals Efek Larvasida Fraksi Etil Asetat Daun Bangun-bangun (Plectranthus amboinicus) terhadap Mortalitas Larva Aedes aegypti

2020 ◽  
Vol 12 (1) ◽  
pp. 27-36
Author(s):  
Sogandi Sogandi ◽  
Fadhli Gunarto
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Acid Group ◽  
Chemical Substance ◽  
Probit Analysis ◽  
Dengue Haemorrhagic Fever ◽  
Haemorrhagic Fever ◽  
Extract Concentration ◽  
Plectranthus Amboinicus

Abstract. Mosquitoes are insects that can transmit diseases to humans through their bites. Dengue haemorrhagic fever is one of the diseases that could be transmitted by Aedes aegypti mosquito. The use of insecticides to control the occurrence of dengue haemorrhagic fever builds resistance of the mosquito towards the chemical substance. Therefore, natural larvicide was used as an alternative to chemical insecticides. This research was conducted to study the effectiveness of larvicides from the ethyl acetate fraction of Bangun-bangun leaves (Plectranthus amboinicus) in killing the Aedes aegypti larvae and to identify the active larvicide compounds. This laboratory experiment research tested six extract concentrations (0.0; 1.0; 1.8; 3.2; 5.6; 10.0%) in three replications. Observations were administered for 48 hours to count the number of the dead Aedes aegypti instar III larvae obtained from the Entomology Laboratory of the Faculty of Veterinary, IPB. The results showed that the larvae mortality increased as the extract concentration increased. The percentage of Aedes aegypti killed reached 96% at an extract concentration of 10%. Meanwhile, the probit analysis showed that LC50 was found at a concentration of 5.56%. The identification of active compounds using GCMS revealed that the larvicidal compounds in ethyl acetate fraction were the ones from an organic acid group which were, stearic acid, and palmitic acid.  Keywords: Aedes aegypti, identification, larvacidal, mortality   Abstrak. Nyamuk merupakan serangga yang dapat menularkan penyakit melalui gigitannya. Salah satu penyakit yang ditularkan adalah demam berdarah dengue yang dibawa oleh nyamuk Aedes aegypti. Pengendalian demam berdarah dengue menggunakan insektisida berbahan kimia dapat menyebabkan resisten nyamuk terhadap insektisida meningkat, oleh karena itu larvasida alami diharapkan dapat menjadi alternatif pengganti insektisida kimia. Tujuan dari penelitian ini adalah mengetahui afektifitas larvasida dari fraksi etil asetat daun Bangun-bangun (Plectranthus amboinicus) terhadap mortalitas larva Aedes aegypti dan mengidentifikasi jenis senyawa aktif yang berperan sebagai larvasida. Penelitian ini merupakan eksperimen laboratorium dengan enam konsentrasi ekstrak (0,0; 1,0; 1,8; 3,2; 5,6; 10,0%) dan tiga kali ulangan. Pengamatan dilakukan selama 48 jam terhadap jumlah kematian larva Aedes aegypti instar III yang diperoleh dari Laboratorium Entomologi Fakultas Kedokteran Hewan, IPB. Hasil penelitian menunjukkan bahwa kematian larva meningkat seiring dengan meningkatnya konsentrasi ekstrak. Kematian Aedes aegypti mencapai 96% pada konsentrasi ekstrak 10% dan hasil analisa probit menunjukkan LC50 terdapat pada konsentrasi 5,56%.  Identifikasi senyawa aktif menggunakan GCMS menunjukkan bahwa senyawa yang bersifat larvasida dalam fraksi etil asetat ini adalah senyawa dari golongan asam organik yaitu, asam stearate, dan asam palmitat.  Kata Kunci: Aedes aegypti, identifikasi, larvasida, mortalitas

scholarly journals The Efficacy of Aedes aegypti Natural Larvicide Gained from Averrhoa bilimbi and Averrhoa carambola

2020 ◽  
Vol 18 (1) ◽  
pp. 92
Author(s):  
GIANINA ANGELIA SANTOSO ◽  
NATHANIA DISA ARIESTA ANDRIANI ◽  
HERBERT ADRIANTO
Keyword(s):  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Probit Analysis ◽  
Laboratory Research ◽  
Mortality Data ◽  
Control Group ◽  
Bioactive Substances ◽  
Averrhoa Carambola ◽  
Post Test ◽  
Experimental Laboratory

<p><strong>ABSTRACT</strong></p><p><strong>Background: </strong>Dengue Hemorrhagic Fever (DHF) is an endemic disease transmitted by Aedes aegypti which is supposed to be demolished by using the Larvicidal activity. However, there are some <em>Ae. aegypti</em> larvae which are found resistant against the temephos in some provinces in Indonesia. Thus, this has been one of the biggest concern of The Ministry of  Health Republic Indonesia. Apart from the resistance effect caused, temephos has been reported causing a water pollution which triggers the decreasing standard of human health and immune system. These factors have created a need for search of a new and natural larvicide, which one of it can be gained from <em>Averrhoa</em>. This research is aimed at comparing the effectiveness between the extract <em>Averrhoa bilimbi</em> and <em>Averrhoa carambola</em> towards the <em>Ae. aegypti</em> larvae’s mortality.</p><p><strong>Method: </strong>An experimental laboratory research involving the design of post test-only control group. Twenty five larvae <em>Ae. aegypti</em> third instar were carried out at 5 different concentrations (0%, 1%, 1.6%, 2.6%, 3.4%, and 4%). The larva demolition is counted within 24 hours. The mortality data is then analysed with the probit analysis.</p><p><strong>Results: </strong>The extract of <em>Averrhoa bilimbi</em> and <em>Averrhoa carambola</em> can cause the mortality of the <em>Ae. aegypti</em> larvae. LC<sub>99 </sub>24 hours from <em>Averrhoa bilimbi</em> was recorded = 1.47% (1.20-2.04%), whereas LC<sub>99 </sub>24 hours was recorded from <em>Averrhoa carambola</em> = 8.96% (7.59-11.31%). The major loses appeared is the stretchy necks and injured appendices. The bioactive substances which are predicted to be the causes of the mortality in this research are saponin glycoside and flavonoid.</p><p><strong>Conclusion: </strong>The extract of <em>Averrhoa bilimbi</em> produces the strongest and the most effective larvicide which will be potentially developed as a new larvicide.</p><p> </p><p>Keywords:<strong> </strong><em>Natural larvicide, Aedes aegypti, Averrhoa bilimbi, Averrhoa carambola</em></p>

scholarly journals Phytochemical analysis, antioxidant, antibacterial, and cytotoxic activities of leaves and roots of Rubus hyrcanus Juz.

2021 ◽  
Author(s):  
Fatemeh Yousefbeyk ◽  
Saeed Ghasemi ◽  
Mehdi Evazalipour ◽  
Sara Dabirian ◽  
Clara Schubert ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Total Phenolics ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Cytotoxic Activities ◽  
Phytochemical Analysis ◽  
Total Extract ◽  
Methanol Fraction

AbstractRubus hyrcanus Juz. (Rosaceae), known as Caspian blackberry, is wildly distributed around the Caspian Sea. This study focused on antioxidant, cytotoxic, and antibacterial activities of total extracts and different fractions from the roots and leaves of this species. The total phenolics and flavonoid contents were also evaluated. Finally, the phenolic profiles of selected fractions were determined using HPLC–DAD and LC–MS/MS. The results indicated that the total phenolics content (TPC) of root total extract (RTE) was 3.5 times that of leaves (340.4 and 102.7 mg GAE/g, respectively). The TPC of three root fractions ranged from 226.6 to 392.9 mg GAE/g, while in leaves fractions, it ranged between 68.3 and 101.8 mg GAE/g. The total extract of leaves had higher contents of total flavonoids than roots (70.5 and 8.9 mg QE/g, respectively). The methanol fractions of both parts had the highest amounts of flavonoids. The root methanol fraction (RMF) had the best antioxidant effect in both DPPH radical scavenging assay (IC50: 9.16 μg ml−1) and total antioxidant capacity test (1010.5 mg ɑTE/g). The RMF and RTE had potent antibacterial activities against Bacillus subtilis and Staphylococcus aureus (MIC 1.5 mg ml−1). In the MTT assay, ethyl acetate fractions of roots and leaves exhibited the best cytotoxicity (IC50 247 and 227 μg ml−1, respectively) and the highest selectivity indexes (4.73 and 5.31, respectively). Phytochemical analysis revealed the presence of gallic acid, p-coumaric acid, and chlorogenic acid in leaves ethyl acetate fraction, chlorogenic acid in leaves methanol fraction, and gallic acid in the root ethyl acetate fraction.

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

scholarly journals Larvicidal Property of the Extract and Fractions of Hannoa klaineana against the Larvae of Aedes aegypti

2020 ◽  
pp. 127-132
Author(s):  
Ifeoma Chioma Ibe ◽  
Eze Elijah Ajaegbu ◽  
Lame Younoussa ◽  
Simon Pierre Yinyang Danga ◽  
Christopher Obidike Ezugwu
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Control Programme ◽  
Chloroform Fraction ◽  
Plant Metabolites ◽  
Lc50 Value ◽  
Synthetic Materials ◽  
Control Programmes ◽  
Crude Methanol Extract

Aedes aegypti (Culicidae) is the main vector for dengue which is endemic in Nigeria and Africa at large. Use of synthetic materials for the control of this vector has always proven abortive. The introduction of plant metabolites which is biodegradable maybe successful and safer to the human health in the control programme of this vector since plants are very rich in bioactive secondary metabolites. Hence, we evaluated the larvicidal activity of the crude methanol extract, n-hexane, chloroform, ethyl acetate and methanol fractions of Hannoa klaineana (Simaroubaceae) against the IV instar larvae of A. aegypti mosquitousing standard protocols. The most active fractions were  n-hexane, chloroform and ethyl acetate fractions with LC50 values of 1.006, 1.479, and 3.198 mg/ml respectively; n-hexane fraction having the highest property with LC50 value of 1.006 mg/ml and followed by chloroform fraction with LC50 value of 1.479 mg/ml. The evaluation has shown that the secondary metabolites of H. klaineana can be used as an alternative for the synthetic larvicides which its use has proven abortive in the vector control programmes.

scholarly journals AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
I Nyoman Pugeg Aryantha ◽  
Wahyu Setyaji Dwiantara
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Beauveria Bassiana ◽  
Larvicidal Activity ◽  
Control Agent ◽  
Probit Analysis ◽  
Hexane Extract ◽  
Potato Dextrose Broth ◽  
Synthetic Pesticides ◽  
Biological Insect Control

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.   

scholarly journals Antidiabetic Activities and Identification of Chemical Compound from Samama (Anthocephalus macrophyllus (Roxb) Havil)

2018 ◽  
Vol 18 (1) ◽  
pp. 66 ◽  
Author(s):  
Laela Nur Anisah ◽  
Wasrin Syafii ◽  
Gustan Pari ◽  
Rita Kartika Sari
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Isopropyl Myristate ◽  
Phytochemical Analysis ◽  
Triterpenoid Saponin ◽  
Ic50 Value ◽  
High Contribution ◽  
Ethanol Extracts

Samama (Anthocephalus macrophyllus) is one of a pioneer indigenous fast growing species in Indonesia which have been used as traditional medicine for various diseases. The objectives were to determine the yield extract, to analyze their antidiabetic activity by inhibition assay for α-glucosidase enzyme activity and chemical analysis with GCMS. Extraction of leaves, bark and wood samples were done by using ethanol 95%. Fractionation the most active  ethanol extract was conducted by using n-hexane and ethyl acetate. The yield of ethanol extracts in leaves, bark and wood were 13.90%, 12.87%, and 2.18% respectively. Based on antidiabetic activity assay, the bark ethanol extract was the most active extract by the IC50 value of 5.86 μg mL-1. Phytochemical analysis on bark ethanol extracts showed that they contained flavonoid, quinon, triterpenoid, saponin and tannin which were assumed have high contribution in antidiabetic activities. The result of fractionation ethanol extract bark showed that the ethyl acetate fraction was the most active fraction (IC50 6.82 μg mL-1). GCMS analysis indicated the presence of dominant phenolic compounds such as pyrocatechol, antiarol, isopropyl myristate and phenol in which were suspected have antidiabetic activity. These results strongly suggested that ethyl acetate fraction of Samama bark was a potential natural source for antidiabetic agents.

AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MANGGIS (Garcinia mangostana L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 525
Author(s):  
Ayu Natasya Paputungan ◽  
Widya Astuty Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Garcinia Mangostana ◽  
Methanol Fraction ◽  
Tlc Bioautography ◽  
Fractionation Method

Mangosteen leaves have flavonoid compounds, tannins, and saponins that can be efficacious as antibacterial. The aim of this study was to determine the fraction of mangosteen leaves having an antibacterial effect and knowing the class of compounds identified as having antibacterial activity after TLC- Bioautography testing was carried out. The samples were extracted using 96% maceratarion method and fractioned using liquid-liquid fractionation method with methanol, n-hexane and ethyl acetate solvents, antibacterial activity using agar diffusion method (Kirby and Bauer) with 3 concetrations namely 10%, 20% and 30%. Thin Layer Chromatography (TLC) uses n-hexane and chloroform solvens. TLC-Bioautography uses contact bioautography methods. The resultd showed that mangosteen leaves in methanol fraction with a concentration of 30% had a very large inhibitory activity again Staphylococcus aureus and ethyl acetate fraction with a concentration of 30% had the gratest antibacterial activity against  Escherichia coli. The results of the TLC- Bioautography study showed that the flavonoids compounds after spraying with AlCl3 and the mangosteen leaf Biosutography test had inhibitory zone activity against the bacteria Staphylococcus aureus dan Escherichia coli. Keywords: Mangosteen Leaves. Antibacterial, TLC Bioautography.  ABSTRAK Daun manggis mempunyai senyawa flavonoid, tanin, dan saponin yang dapat berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui fraksi daun manggis memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT Bioautografi. Sampel diektrak dengan metode maserasi dengan pelarut 96% dan difraksinasi dengan metode  fraksinasi cair-cair dengan pelarut metanol, n-heksan dan etil asetat, aktivitas antibakteri menggunakan metode difusi agar (Kirby and Bauer) dengan 3 kosentrasi yaitu 10%, 20% dan 30%. Kromatografi Lapis Tipis (KLT) menggunakan pelarut n-heksan dan klorofom. KLT-Bioautografi menggunakan metode bioautografi kontak. Hasil penelitian menunjukan daun manggis pada fraksi metanol  dengan kosentrasi 30% memiliki aktivitas zona hambat ppaling besar terhadapat bakteri Staphylococcus aureus dan fraksi etil asetat dengan kosentrasi 30% memiliki aktivitas antibakteri paling besar terhadap  Escherichia coli. Hasil penelitian KLT-Bioautografi menunjukan golongan senyawa flavonoid setelah disemprotkan dengan AlCl3 dan uji Bioautografi daun manggis memiliki aktivitas zona hambat terhadap bakteri Staphylococcus aureus dan Escherichia coli. Kata kunci : Daun Manggis, Antibakteri, KLT- Bioautografi.

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