Kualitas Semen Beku Sexing Sapi Peranakan Ongole  Menggunakan Volume Semen Awal Yang Berbeda

ABSTRAKInseminasi buatan dengan menggunakan semen sexing diharapkan menghasilkan pedet dengan jenis kelamin sesuai harapan. Tujuan dari penelitian ini adalah untuk mengetahui kualitas, proporsi, dan jumlah produksi straw sexing menggunakan metode sentrifugasi gradien densitas percoll dengan volume awal semen yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong, Kecamatan Grati, Kabupaten Pasuruan dan Laboratorium Reproduksi Ternak, Fakultas Peternakan Universitas Brawijaya, Malang. Materi yang digunakan adalah semen sapi peranakan ongole berumur berkisar lima tahun dan bobot badan berkisar 700 kg sebanyak tiga ekor, motilitas massa ≥2+ dan motilitas individu ≥70%. Metode yang digunakan adalah eksperimental dengan tiga perlakuan volume awal saat sexing, yaitu 1 (P1); 1,5 (P2); dan 2 (P3) ml dengan ulangan 11 kali (ulangan berfungsi sebagai kelompok). Data dianalisa menggunakan Rancangan Acak Kelompok (RAK). Hasil penelitian menunjukkan bahwa perbedaan volume awal semen tidak berpengaruh (menurun) terhadap motilitas, viabilitas, abnormalitas, konsentrasi, total spermatozoa motil, recovery rate dan proporsi spermatozoa (P>0,05). Pengaruh yang sangat nyata (meningkat) terhadap jumlah produksi straw (P<0,01). Ulangan penelitian ini memberikan pengaruh yang sangat nyata (meningkat) terhadap kualitas (motilitas, konsentrasi, viabilitas, abnormalitas, total spermatozoa motil, RR, proporsi dan jumlah straw) dan proporsi spermatozoa X dan Y (P<0,01). Total spermatozoa motil setiap perlakuan telah memenuhi nilai harapan (10 juta/straw). Proporsi spermatozoa X dan Y telah memenuhi nilai harapan (80%:20%).Kata Kunci: kualitas, proporsi, semen beku sexing, strawABSTRACTArtificial insemination using sexing semen is expected to produce calves with the expected sex. The aim of this study was to determine the quality, proportion, and quantity of sexing semen production using the percoll density gradient centrifugation method with different initial semen volumes. The research was conducted at the Beef Cattle Research, Grati District, Pasuruan Regency, East Java Province, Indonesia, and the Animal Reproduction Laboratory, Faculty of Animal Science, University of Brawijaya, Malang, East Java Province, Indonesia. The material used was semen from three Ongole crossbred bull aged around five years and the bodyweight of around 700 kg, mass motility of ≥2+, and individual motility ≥70%. The method used was experimental with three initial volume treatments during sexing, namely 1 (P1); 1.5 (P2), and 2 (P3) ml with 11 replications (replications function as groups). The data were analyzed using a randomized block design (RBD). The results showed that the treatment of differences in initial semen volume did not affect motility, viability, abnormalities, concentration, total motile sperm, recovery rate, and proportion of sperm (P>0.05). On the other hand, the difference in the initial volume of semen had a very significant effect (increased) on the amount of frozen semen production (P<0.01). Repeated research also had a very significant effect (increased) on the semen quality (motility, concentration, viability, abnormality, total sperm motility, recovery rate proportion, and straw production) and the proportion of spermatozoa X and Y (P<0.01). The total motile sperm for each treatment had met the expected value (10 million/straw). Proportions of spermatozoa X and Y have met the expected value (80%: 20%).Keywords: proportion, quality, sexing frozen semen, straw.

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Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5449 ◽

2019 ◽

Vol 6 (1) ◽

pp. 78

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nur Isnaini ◽

Trinil Susilawati

Keyword(s):

Semen Quality ◽

Block Design ◽

Animal Husbandry ◽

Egg Yolk ◽

Coconut Water ◽

Boer Goat ◽

Randomized Block Design ◽

Frozen Semen ◽

Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari 3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1: young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%), while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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Comparative study of semen quality traits between Etawah grade and Senduro bucks

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/888/1/012022 ◽

2021 ◽

Vol 888 (1) ◽

pp. 012022

Author(s):

N Isnaini ◽

A A Hakim ◽

D Amertaningtyas ◽

H E Sulistyo ◽

A Irsyammawati ◽

...

Keyword(s):

Comparative Study ◽

Artificial Insemination ◽

Recovery Rate ◽

Semen Quality ◽

T Test ◽

Quality Traits ◽

Spermatozoa Motility ◽

Frozen Semen ◽

Semen Volume

Abstract This study compared the semen quality traits between Etawah grade (EGB) and Senduro bucks (SB) raised in Singosari National Artificial Insemination Center, Indonesia. A total of 96 ejaculated semen were collected from three EGB and three SB with an average age of three years. The ejaculates were evaluated for semen volume (SV), spermatozoa concentration (SC), total spermatozoa (TS), spermatozoa motility (SM), post-thawing spermatozoa motility (PTSM), the recovery rate of spermatozoa motility (RRSM), and frozen semen production (FSP). Data of SV, SM, PTSM, and RRSM were compared using the Mann-Whitney U test. While the data of SC, TS, and FSP were analyzed using an independent t-test. The results showed that SV, SM, PTSM, and RRSM were not significantly differed (P>0.05) between breeds. Whereas, SC, TS, and FSP of EGB were significantly higher (P<0.05) than SB. Therefore, it can be concluded that Etawah grade bucks can produce higher total spermatozoa and as a result higher frozen semen production for artificial insemination as compared to Senduro bucks.

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18 SEMENRATE: THE USE OF COMPUTER-ASSISTED SEMEN ANALYSIS AND FLOW CYTOMETRY FOR OBJECTIVE BOVINE SEMEN ANALYSIS IN THE UNITED KINGDOM

Reproduction Fertility and Development ◽

10.1071/rdv29n1ab18 ◽

2017 ◽

Vol 29 (1) ◽

pp. 116

Author(s):

M. W. Spilman ◽

K. L. Burton ◽

J. M. E. Statham

Keyword(s):

Flow Cytometry ◽

United Kingdom ◽

Semen Quality ◽

Semen Analysis ◽

Computer Assisted ◽

Motile Sperm ◽

Bovine Semen ◽

The United Kingdom ◽

Frozen Semen ◽

Viable Sperm

Routine assessment of bovine semen consists of a subjective assessment of morphology, motility and concentration. This subjective approach used during quality control at semen production centres (SPC) or investigations of poor reproductive performance in veterinary practice has been shown to be relatively inaccurate, imprecise, and operator dependent (Vincent, et al. 2012 Anim. Reprod. 9, 153–165). Assessment of frozen semen samples in a dedicated laboratory aimed to establish variations in multiple parameters associated with fertility using computer-assisted semen analysis and flow cytometry and evaluate their relationship to semen performance in the field. This has developed into a commercial service that is available to veterinarians and farmers across the United Kingdom. AI semen from 50 farms across Yorkshire, UK, that had been stored on farm was assessed for factors associated with fertility (motility, progressive motility, intact acrosome, viability, and polarised mitochondria). Data ranges and mean values for each parameter have been analysed. This analysis is ongoing as the dataset continues to expand and significance will be assessed. For frozen semen (n = 79), % viable sperm (max = 67.64, min = 0.00, mean = 43.44), % sperm with polarised mitochondria (max = 72.50, min = 0.26, mean = 38.56), % sperm with acrosome intact (max = 68.82, min = 0.06, mean = 35.29), % motile sperm (max = 66.90, min = 0.00, mean = 37.44) and % progressively motile sperm (max = 59.00, min = 0.00, mean = 26.11). 25% of the samples fell below the cut off for release of 30% motile sperm set by SPCs. For sexed AI semen (n = 9), % viable sperm (max = 66.31, min = 17.08, mean = 43.57), % polarised mitochondria (max = 26.74, min = 13.40, mean = 19.96), % intact acrosome (max = 52.62, min = 15.34, mean = 37.00), % motile (max = 38.00, min = 9.40, mean = 24.88) and % progressively motile (max = 22.80, min = 3.90, mean = 13.15). Objective semen analysis before beginning an embryo collection programme allows informed decisions to be made regarding semen choice and dosage depending on compensable v. non-compensable defects detected (Hudson et al. 2012 Dairy Herd Health 73–111; CABI Publishing). Use of semen that falls below the 30% cut off for SPCs is unlikely to perform as expected in the field (Phillips et al. 2004 Anim. Reprod. Sci. 80, 47–61). A European collaboration aims to establish correlations between semen quality parameters and fertility outcomes for UK cattle herds, providing unique data for the industry (Sellem et al. 2015 Theriogenology 84, 1447–1454.e5). These data should highlight to stakeholders in the industry how imperative optimal semen quality is and highlight the benefits to herd fertility and financial performance.

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The Influence of Breed and Type of Extender on the Quality of Bull Semen

ANIMAL PRODUCTION ◽

10.20884/1.jap.2019.21.2.641 ◽

2020 ◽

Vol 21 (2) ◽

pp. 64

Author(s):

Iman Sukirman ◽

Eros Sukmawati ◽

Siti Darojah Rasad ◽

Nurcholidah Solihati

Keyword(s):

Plasma Membrane ◽

Semen Quality ◽

Block Design ◽

Egg Yolk ◽

Spermatozoa Motility ◽

Bull Semen ◽

Randomized Block Design ◽

Frozen Semen ◽

Two Factors

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

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Keberhasilan inseminasi buatan menggunakan semen sexing beku pada Sapi Persilangan Ongole

Jurnal Ilmu-Ilmu Peternakan ◽

10.21776/ub.jiip.2018.028.03.09 ◽

2018 ◽

Vol 28 (3) ◽

pp. 259 ◽

Cited By ~ 1

Author(s):

Dwi Susan Setiyani ◽

Aulia Puspita Anugra Yekti ◽

Kuswati Kuswati ◽

Trinil Susilawati

Keyword(s):

Success Rate ◽

Artificial Insemination ◽

Density Gradient Centrifugation ◽

Gradient Centrifugation ◽

Return Rate ◽

Percoll Density Gradient Centrifugation ◽

Frozen Semen ◽

Percoll Density Gradient ◽

Density Gradient Centrifugation Method ◽

Centrifugation Method

The Artificial Insemination (AI) results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate1 (NRR1), Non Return Rate2 (NRR2) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR1 and NRR2AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR1 value is 73,91%; 93,54% while the value of NRR2 was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.

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Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.43.1.26-34 ◽

2018 ◽

Vol 43 (1) ◽

pp. 26 ◽

Cited By ~ 1

Author(s):

H. C. Mahendra ◽

D. Samsudewa ◽

Y. S. Ondho

Keyword(s):

Artificial Insemination ◽

Recovery Rate ◽

Semen Quality ◽

Membrane Integrity ◽

Animal Husbandry ◽

Mean Value ◽

National Standard ◽

Frozen Semen ◽

Standard Values

This study aimed to evaluate and compare the quality of post thawing spermatozoa of buffalo frozen semen produced by artificial insemination centers on standard values, and proposed reference values (PRV). Materials of the research were 60 samples of straws obtained from three Artificial Insemination Center, which are each 20 straws, respectively. Parameters observed were motility, concentration, longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and recovery rate. The obtained data were tested by Z test then presented as mean ± standard deviation. The research results from three artificial insemination center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11 x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was 45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%. The conclusions of this research were the motility and concentration mean were higher than Indonesian National Standard (INS), longevity mean was lower than Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI and AIn mean were higher, the mean value of recovery rate was higher than of proposal value.

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Variability in semen quality from British sport horses

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200019323 ◽

2007 ◽

Vol 2007 ◽

pp. 29-29

Author(s):

L.A. Tucker ◽

T. Matson ◽

L.H.A. Morris

Keyword(s):

Semen Quality ◽

Motile Sperm ◽

Ongoing Research ◽

Progressive Motility ◽

Worldwide Distribution ◽

Management Interventions ◽

Frozen Semen ◽

Quality Assessments ◽

The Impact ◽

Fertility Traits

Recent advances in artificial insemination (AI) and semen preparation have led to an increase in the availability of equine genetics globally. Generally, less than half the stallion population produce semen of suitable robustness to maintain acceptable fertility post-freezing, and research is needed to improve not only the numbers of stallions whose semen can be successfully frozen, but also to increase the number of collections that meet fertility standards. The number of progressively motile sperm present during ovulation determines successful conception in horses. AI practitioners recommend that 500 million progressively motile sperm should be introduced into the mare’s uterus to ensure best chance of pregnancy (Matson and Pycock, 2006), and progressive motility is currently accepted as suitable for determining fertility in stallions. Established laboratory quality assessments are used to identify stallions with suitably robust semen for freezing and worldwide distribution, however fertility traits can be influenced by many factors, e.g. age, level of competitive performance or workload, nutrition, disease and stress (Tucker and Morris, 2006). In order to investigate the impact of such factors on semen quality, it is first important to determine the level of variation in semen quality between stallions that are currently used for frozen semen collections, which formed the main objective of the following study. This baseline can then be used to compare improvements in semen quality from various nutritional and management interventions, which form the basis of our ongoing research program.

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Seminal Attributes, Freezability and their Interrelationships in Zebu Cattle and Buffalo Bulls from Central Gujarat

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.14.2.1 ◽

2018 ◽

Vol 14 (2) ◽

Author(s):

P. K. Pathak ◽

A. J. Dhami ◽

D. V. Chaudhari

Keyword(s):

Semen Quality ◽

Conventional Technique ◽

Zebu Cattle ◽

Motile Sperm ◽

Morphological Attributes ◽

Frozen Semen ◽

The Mean ◽

Live Sperm ◽

Sperm Output ◽

Fresh Semen

A study was carried out on nine healthy mature breeding bulls (3 each of Gir, Surti and Murrah breed) to evaluate their fresh and frozen semen quality and their interrelationships. The ejaculates immediately after collection were evaluated for routine physico- morphological attributes, including HOS test. The ejaculates (n=72) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and the French mini straws filled were frozen in liquid nitrogen vapour using a programmable biofreezer. Thawing of straws was done at 37°C for 30 sec and assessed for freezability by conventional technique. All the cattle and buffalo bulls donated consistently normal thick creamy yellow and thick milky white semen, respectively. In Gir, Suti and Murrah bulls (n=24 ejaculate each) the seminal attributes such as ejaculate volume (6.69±0.17, 3.12±0.10 and 3.96±0.16 ml, p less than 0.01); initial motility (80.21±0.88, 84.58±0.60 and 84.38±0.76 %, p less than 0.01); total sperm output/ejaculate (9013.85±265.32, 3935.49±259.63 and 5366.48±332.99 million, p less than 0.01) and live sperm (84.71±0.83, 86.17±0.78 and 86.79±0.79 %, p less than 0.05) differed significantly. The mean percentages of post-thaw motile sperm (53.29±1.56, 58.33±1.43 and 59.58±1.20, p less than 0.01); live sperm (59.00±1.95, 67.00±1.59 and 68.42±1.66 %, p less than 0.01); and HOS reactive sperm (48.25±0.78, 44.21±1.29 and 51.54±1.29 %, p less than 0.01) in Gir, Surti and Murrah bulls semen also differed significantly. The variation among the bulls was significant for buffalo breeds in most of their fresh seminal attributes, except HOST, and for post-thaw motility, but not among Gir bulls. The important seminal attributes like motility, live sperm and HOS reactive sperm of fresh and frozen-thawed semen were significantly and positively interrelated in all three breeds of bulls (r = 0.40 to 0.81, p less than 0.05 to 0.01), suggesting that motility and HOST of fresh semen were good predictors of freezability of bovine semen.

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Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Tropical Animal Science Journal ◽

10.5398/tasj.2021.44.2.146 ◽

2021 ◽

Vol 44 (2) ◽

pp. 146-151

Author(s):

Nurcholis Nurcholis ◽

A. Furqon ◽

R. I. Arifiantini ◽

S. M. Salamony

Keyword(s):

Sperm Motility ◽

Recovery Rate ◽

Semen Quality ◽

Egg Yolk ◽

Sperm Viability ◽

Nitrogen Vapor ◽

Frozen Semen ◽

Fruit Oil ◽

Range Test

Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

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Semen Production Characteristics of Pasundan Bull at Different Body Weight

Jurnal Agripet ◽

10.17969/agripet.v21i1.15186 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Anggit Damaratri Lapoliwa ◽

Nurul Isnaini

Keyword(s):

Body Weight ◽

Sperm Motility ◽

Recovery Rate ◽

Sperm Concentration ◽

The Body ◽

Production Traits ◽

Motile Sperm ◽

Frozen Semen ◽

Semen Volume ◽

Production Characteristics

ABSTRACT. Pasundan cattle is currently proposed as a potential livestock to support national meat self-sufficiency program. However, the information regarding their reproductive performance is still very limited. This study was conducted to evaluate the semen production characteristics of Pasundan bull at different body weight. A total of 178 semen samples which were collected from one Pasundan bull were used in this study. The semen collection was done for 31 months and during this period, the body weight of Pasundan bull was classified into 4 categories, namely 400 kg, 400 to 500 kg, 500 to 600 kg, and ≥600 kg. The results showed that overall mean semen volume, semen pH, sperm concentration, total sperm, individual sperm motility, total motile sperm, post-thawing sperm motility, recovery rate of sperm motility, and frozen semen production were 5.89 ml/ejaculate, 6.69, 1.04 billion/ml, 6.04 billion/ejaculate, 55.75%, 3.40 billion/ejaculate, 40.91%, 58.20%, and 265.11 doses/ejaculate. The difference in body weight significantly affect semen volume (P=0.001), semen pH (P=0.001), sperm concentration (P=0.043), total sperm (P=0.002), individual sperm motility (P0.001), total motile sperm (P0.001), and frozen semen production (P=0.004). There was no significant effect (P0.05) of body weight on post-thawing sperm motility and recovery rate of sperm motility. In conclusion, the semen production traits of Pasundan bull are improved with the increase in body weight up to 500 to 600 kg and remain stable at the body weight of ≥600 kg. (Karakteristik produksi semen sapi Pasundan pada bobot badan yang berbeda) ABSTRAK. Sapi Pasundan saat ini diajukan sebagai salah satu ternak potensial untuk mendukung program swasembada daging nasional. Akan tetapi, informasi tentang penampilan reproduksinya masih sangat terbatas. Penelitian ini dilakukan untuk mengevaluasi karakteristik produksi semen sapi pejantan Pasundan pada bobot badan yang berbeda. Sebanyak 178 sampel semen yang dikoleksi dari 1 ekor sapi pejantan Pasundan digunakan pada penelitian ini. Penampungan semen dilakukan selama 31 bulan dan pada periode tersebut, bobot badan sapi pejantan Pasundan diklasifikasikan menjadi 4 kategori yaitu 400 kg, 400 to 500 kg, 500 to 600 kg, and ≥600 kg. Hasil penelitian menunjukkan bahwa rata-rata volume semen, pH semen, konsentrasi sperma, total sperma, motilitas individu sperma, total sperma motil, motilitas sperma post-thawing, nilai recovery rate, dan produksi semen beku adalah 5,89 ml/ejakulat, 6,69, 1,04 miliar/ml, 6,04 miliar/ejakulat, 55,75%, 3,40 miliar/ejakulat, 40,91%, 58,20%, dan 265,11 dosis/ejakulat. Perbedaan bobot badan memberikan pengaruh terhadap volume semen (P=0,001), pH semen (P=0,001), konsentrasi sperma (P=0,043), total sperma (P=0,002), motilitas individu sperma (P0,001), total sperma motil (P0,001), dan produksi semen beku (P=0,004). Di sisi lain, bobot badan tidak memberikan pengaruh (P0,05) terhadap motilitas sperma post-thawing dan nilai recovery rate. Kesimpulannya, karakteristik produksi semen sapi pejantan Pasundan meningkat seiring dengan peningkatan bobot badan hingga 500 sampai 600 kg dan tetap stabil hingga ≥600 kg.

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