scholarly journals Mozart Memberikan Hasil Indeks Apoptosis Lebih Rendah daripada Musik Pop, Religi dan Tanpa Pemberian Musik

2019 ◽  
Vol 11 (1) ◽  
pp. 28
Author(s):  
Dian Sukmawati ◽  
Hermanto Tri Juwono ◽  
Widjiati Widjiati
Keyword(s):  
Rattus Norvegicus ◽  
Classical Music ◽  
Fetal Brain ◽  
Control Group ◽  
Pop Music ◽  
Religious Music ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Post Test ◽  
Neuron Apoptosis

Background: Brain growth requires genetic potential for a conducive environment, low stress levels, stimulation and nutrition. Brain development during the fetal period in the two years of life firstly requires proper stimulation to increase intelligence. Classical music stimulation has been proven to be able to optimally improve brain function and human intellect. Other music that Indonesian people like is pop and religion. Maybe it is possible to pop and religious music can affect the development and growth of the fetal brain. Objective: Analyze the differences of the index of apoptosis Cerebrum and Cerebellum Rattus norvegicus newborn between those stimulated by Mozart, pop, religious music and not stimulated by music during pregnancy. Method: This study was post test only control group design. groups were randomly divided according to the treatment as stimulation of Mozart, pop, religious music and no music was given from the 10th day-gestation with an intensity of 65 dB in a soundproof box for one hour. When 19th day-gestation, Rattus norvegicus mothers were sacrificed then 3 Rattus norvegicus newborn from each mothers were selected and brain was taken to be made immunohistochemical preparations and counted the number of neuron cells apoptosis index. data were analyzed by comparison test with p <0.05. Results: There was a significant difference in the cerebrum neuron apoptosis index newborn between groups p = 0.001 but there was no difference between without exposure and pop music groups p = 0.063 (the lowest mean was the mozart group 2.40 IRS) and there was also difference in the apoptosis index in the cerebellum p = 0,000 but there was no difference between pop music and without exposure group p = 0.151 (the lowest mean was the mozart group 2.34 IRS) Conclusion: Mozart gives a lower apoptotic index than pop music, religion and without exposure music group. Keywords: neuron apoptosis index; mozart mucic; pop music; religion music ABSTRAK Latar Belakang: Tumbuh kembang otak membutuhkan potensi genetik lingkungan yang kondusif, tingkat stres yang rendah, stimulasi dan nutrisi. Perkembangan otak selama periode janin dan dua tahun pertama kehidupan memerlukan stimulasi yang tepat untuk meningkatkan kecerdasan. Stimulasi musik klasik terbukti dapat meningkatkan fungsi otak dan intelektual manusia secara optimal. Musik lain yang suka didengarkan oleh masyarakat Indonesia adalah pop dan religi. Kemungkinan musik pop dan religi juga dapat mempengaruhi perkembangan dan pertumbuhan otak janin. Tujuan: Menganalisis perbedaan indeks apoptosis Cerebrum dan Cerebellum Rattus norvegicus baru lahir antara yang di stimulasi musik Mozart, pop, religi dan tidak di stimulasi musik selama kebuntingan. Metode: Penelitian ini adalah post test only control grup design. kelompok dibagi secara acak disesuaikan dengan perlakuan yaitu pemberian musik Mozart, pop, religi dan tidak diberikan musik sejak hari ke-10 kebuntingan dengan intensitas 65 dB dalam kotak kedap suara selama satu jam. Kehamilan hari ke 19 tikus bunting dikorbankan, anak tikus dipilih 3 ekor tiapinduk diambil otaknya, dibuat sediaan imunohistokimia dan dihitung jumlah sel neuron yang mengalami apoptosis. Hasil dianalisis uji perbandingan dengan komparasi p<0,05. Hasil: Terdapat perbedaan bermakna indeks apoptosis cerebrum Rattus norvegicus baru lahir antar kelompok p=0,001 namun tidak ada perbedaan kelompok tanpa paparan dengan musik pop p=0,063 (mean terendah adalah kelompok mozart 2,40 IRS) dan terdapat perbedaan indeks apoptosis di cerebellum dengan nilai p=0,000 namun tidak ada perbedaan kelompok tanpa paparan dengan musik pop p=0,151 (mean terendah adalah kelompok mozart 2,34 IRS) Kesimpulan : Mozart memberikan hasil indeks apoptosis lebih rendah dari musik pop, religi dan tanpa pemberian musik. Kata kunci: Indeks apoptosis; musikmozart; musik pop; musik religi

scholarly journals Synapsis Expression Increased By Mozart’s Music Stimulation Compared With Indonesian Pop And Religious Music

2020 ◽  
Vol 16 (1) ◽  
pp. 28
Author(s):  
Ukhti Mukminah ilmi Amila ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Rattus Norvegicus ◽  
Classical Music ◽  
Control Group ◽  
Pop Music ◽  
Religious Music ◽  
Significant Difference ◽  
Soundproof Chamber ◽  
The Difference ◽  
Experimental Treatments ◽  
The Brain

Classical music stimulation is a proven method that affect intelligence as well as brain function. Mozart’s classical music is classical music that could stimulate the growth of synaptic networks. To analyze the difference of synapsin expression of the brain of Rattus norvegicus  offspring on Mozart’s, pop, religious and without music stimulation during pregnancy. Experimental. Treatments were subjected to Rattus norvegicusas a subject organism, initiated on the 10th day of pregnancy, in 1 hour duration in a soundproof chamber, intensity of 65 dB. On the 19th day of pregnancy the subjects were sacrificed and three offsprings were chosen. The head of the chosen Rattus norvegicusoffsprings were decapitated and the brain subsequently sectioned to calculate synapsin expression by immunohistochemistry method with 400 magnification strength microscope on the 5 field of view. Significant difference of synapsin expression of the brain of Rattus norvegicus offspring was observed between Mozart’s and control group. Significant differences of synapsin expressions were observed in the cerebellum of the newly born Rattus novergicus between Mozart and pop music groups (p = 0,007), Mozart and religious music groups (p = 0,008), Mozart and without music groups (p = 0,004).Synapsin expression of Rattus norvegicus brain offsprings were higher in Mozart music stimulation compared with pop and religious music stimulation

scholarly journals Jumlah Sel Neuron Cerebrum pada Paparan Musik Mozart Lebih Tinggi Dibandingkan Dengan Paparan Musik Indonesia

2019 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Agustina Mar'atus Sholichah ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Fetal Brain ◽  
Control Group ◽  
Pop Music ◽  
Test Results ◽  
Control Group Design ◽  
Religious Music ◽  
Group Design ◽  
Post Test ◽  
Music Stimulus ◽  
Hematoxylin Eosin

Background: Intelligence management can produce superior human resources with integrated brain. Mozart music stimulus during pregnancy has been shown to increase the number of neuron of the fetal brain. The study of pop and religious music needs to be improve because they are more popular in Indonesia. Objective: To analyze the differences of number of Neuron in the Cerebrum Rattus norvegicusoffspring that exposed to Mozart, Indonesian pop music and Indonesian religious music during pregnancy. Methods: An experimental study with a post-test only control group design. Groups divide into treatment music groups: Mozart, pop and religious. Treatment in a soundproof room for 1 hour, starting the 10th-day of pregnancy, intensity of 65 dB with a distance of 25 cm from the cage. The number of neuron was counted from HE brain preparations of the head Rattus norvegicusoffspring and analyzed using appropriate statistics test. Results: There were significant differences in the number of neuron of Rattus norvegicusoffspring in cerebrum between groups with p = 0,000 (mean Mozart music group 28.14 ± 3.02, Indonesian pop music 19.71 ± 1.80, Indonesian religious music 24.14 ± 2.91) and Mozart gave a higher number of neuron than Indonesia religious music and Indonesian pop music. Conclusion: Mozart music gave a higher number of neuron in the Cerebrum than Indonesian religious music and Indonesian pop music. Keywords: neuron; cerebrum; Mozart music; Indonesian music ABSTRAK Latar belakang: Pengelolaan kecerdasan otak yang terintegrasi akan menghasilkan SDM yang unggul. Stimulus musik Mozart selama kehamilan terbukti meningkatkan jumlah sel neuron di otak janin. Musik pop dan religi perlu dilakukan penelitian karena lebih populer di Indonesia. Tujuan: Menganalisis perbedaan jumlah sel neuron di Cerebrum Rattus norvegicusbaru lahir antara yang mendapat paparan musik Mozart, musik pop Indonesia dan musik religi Indonesia selama kebuntingan. Metode: Studi eksperimental dengan desain post test only control group. Kelompok perlakuan dibagi menjadi kelompok musik Mozart, musik pop Indonesia dan musik religi Indonesia. Perlakuan di ruang kedap suara selama 1 jam pada malam hari mulai hari ke-10 kebuntingan, intensitas 65 dB dengan jarak 25 cm antara kandang dan speaker. Jumlah sel neuron dihitung dari preparat pewarnaan Hematoxylin-Eosin otak anak Rattus norvegicusdan dianalisis dengan statistik yang sesuai. Hasil: Terdapat perbedaan yang signifikan pada jumlah sel neuron cerebrum Rattus norvegicus baru lahir antar kelompok dengan nilai p=0,000 (rerata kelompok musik Mozart 28,14±3,02, musik pop Indonesia 19,71±1,80 dan musik religi Indonesia 24,14±2,91) dan musik Mozart memiliki jumlah sel neuron lebih tinggi daripada musik religi Indonesia dan musik pop Indonesia. Kesimpulan: Kelompok musik Mozart memiliki jumlah sel neuron di cerebrum yang lebih tinggi dibandingkan kelompok musik Indonesia. Kata kunci: neuron; cerebrum; musik Mozart; musik Indonesia

scholarly journals Apoptosis index of cerebrum and cerebellum neuronal cells in Rattus norvegicus neonates born from mothers treated with 50% food restriction during gestation

2018 ◽  
Vol 26 (2) ◽  
pp. 48
Author(s):  
Andita Hapsari ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Rattus Norvegicus ◽  
Food Restriction ◽  
Apoptotic Index ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Post Test ◽  
The Difference ◽  
And Control ◽  
Veterinary Faculty

Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.

scholarly journals Effect of per oral sipermetrin exposure on serum 17 estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

scholarly journals Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

2018 ◽  
Vol 2 (1) ◽  
pp. 24-40
Author(s):  
Adi Wijayanto
Keyword(s):  
Rattus Norvegicus ◽  
Exercise Program ◽  
The Body ◽  
Control Group ◽  
Regular Exercise ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

scholarly journals Mozart Music Increases The Number Of Glial Cells Compared To Indonesia Pop And Religious Music

2019 ◽  
Vol 15 (2) ◽  
pp. 133
Author(s):  
Pipit Sri Estuning Rahayu ◽  
Hermanto Hermanto ◽  
Widjiati Widjiati
Keyword(s):  
Glial Cells ◽  
Rattus Norvegicus ◽  
P Value ◽  
Pop Music ◽  
Religious Groups ◽  
Religious Music ◽  
Significant Difference ◽  
Soundproof Chamber ◽  
Exposure During Pregnancy ◽  
The Brain

Music stimulation is an important component for prenatal fetal development. Both pop and religious music are easy to listen and widely accepted in Indonesia. This study was to analyze the effect of Mozart, pop, and religious music exposure during pregnancy to the number of glial cells in the brain of Rattus norvegicus offspring. The samples were divided into three groups based on the exposure for each group, namely Mozart, pop, and religious music, duration of 60 minutes with 65dB intensity, initiated on the 10th day of pregnancy for 9 days in the soundproof chamber. Three brains of the offsprings were dissected and prepared for Hematoxylin-Eosin staining counted on 5 fields of view and 400 magnification strength.Different glial cells number of Rattus norvegicus brain between groups were observed. Mozart music (28,29) showed a highest mean and pop music (18,67)  showed the lowest mean. Significant difference of the number of brain glial cells between Mozart music compared to pop and religious music groups were observed, with p value <0,005.The number of brain glial cells of Rattus norvegicus offsprings in the Mozart group were significantly higher than those in pop and religious groups.

scholarly journals Default sequence of Mozart’s compositions during pregnancy gave higher dendritic density in the cerebrum and cerebellum of Rattus norvegicus offsprings compared with reversed sequence and control

2019 ◽  
Vol 27 (1) ◽  
pp. 5
Author(s):  
Harry Mangasi Binsar Panjaitan ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati
Keyword(s):  
Experimental Study ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Exposure Group ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Objectives: This study aim to determine sequence of Mozart songs in the analyzing differences in dendritic density of cerebellum and cerebellum of new born Rattus novergicusMaterials and Methods: Experimental study randomized post test only control group design using Rattus norvegicus. Animal subjects were divided into three groups which were control group, and the treatment group that were given exposure to default sequence and reversed sequence of Mozart's music from gestation day 10. We used a comparison test in the analysis expression of BDNF.Results: In the cerebrum there was a significant difference in the Mozart group in reverse rather than with Mozart standard sequence and in standard Mozart group with no exposure group, with p=0.003 and p=0.000. In the cerebellum there was a significant difference in the group in reverse rather than with standard Mozart and in the standard Mozart group with the control group, with p=0.000 and p=0.000. However, there was no significant difference between control group and Mozart group upside down in cerebrum and cerebellum with p=0.109 and p=0.077Conclusion: The density of dendrites in the cerebrum and cerebellum of Rattus norvegicus newborn exposed to Mozart's music during pregnancy with standard Mozart sequence was higher than that in those receiving reverse order and without exposure. There were no significant differences between the density of the cerebrum and cerebellum dendrites between groups exposed to reversed sequence of Mozart composition and those without exposure.

scholarly journals Effect of per oral sipermetrin exposure on serum 17-beta estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

2018 ◽  
Vol 26 (1) ◽  
pp. 20
Author(s):  
Hesty Widowati ◽  
Hidayat Sujuti ◽  
Karyono Mintaroem
Keyword(s):  
Rattus Norvegicus ◽  
Serum Levels ◽  
Female Rats ◽  
Control Group ◽  
Serum Estradiol ◽  
Opposite Pattern ◽  
Wistar Strain ◽  
Significant Difference ◽  
Post Test

Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).

scholarly journals Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons

2018 ◽  
Vol 25 (3) ◽  
pp. 97
Author(s):  
Eka Nasrur Maulana ◽  
Hermanto Tri Joewono ◽  
Widjiati Widjiati ◽  
Windhu Purnomo
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Apoptotic Index ◽  
Assay Method ◽  
Optimal Time ◽  
Control Group ◽  
Apoptosis Index ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control group's neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found  the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.

scholarly journals Fifty percent of food restriction during gestation reduced the dendritic density of cerebrum and cerebellum of Rattus norvegicus newborn

2019 ◽  
Vol 26 (3) ◽  
pp. 112
Author(s):  
Anwar Fauzi ◽  
Widjiati Widjiati ◽  
Hermanto T Joewono
Keyword(s):  
Rattus Norvegicus ◽  
Food Restriction ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Group Design ◽  
Treatment Models ◽  
Significant Difference ◽  
Post Test ◽  
Single Blind

Objectives: To analyze the influence of 50 percent food  restriction during pregnancy to the dendritic density of cerebellum and cerebellum of newborn Rattus norvegicus.Materials and Methods: Laboratory experimental study with single blind randomized post-test only control group design using animal model; pregnant Rattus norvegicus as treatment models. Subjects were divided into two groups: control group and treatment group which was exposed to 50% food restriction (FR 50%). At day 21, both group sacrificed and the cerebrum and cerebelum of the offsprings were prepared and stained with silver impregnation. We used parametric independent t-test in analyzing dendritic density.Results: In the cerebrum there was a significant difference in dendritic density between control (4.98+2.17) and treatment (2.69+0.76) groups with p=0.001 (p<0.05). In the cerebellum there was ALSO a significant difference in dendritic density between control (7.37+2.23) and treatment groups (3.01+0.64) with p=0.000 (p<0.05).Conclusions: The dendritic density of cerebrum and cerebellum of newborn Rattus norvegicusexposed to 50 percent of food restriction during pregnancy  were lower than control. 

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