scholarly journals Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line

2021 ◽  
Vol 9 ◽  
Author(s):  
Neelam Mishra ◽  
Kavita Rana ◽  
Siva Deepthi Seelam ◽  
Rakesh Kumar ◽  
Vijyendra Pandey ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Vital Role ◽  
Cancer Targeting ◽  
Dpph Assay ◽  
Ncbi Accession ◽  
Diphenyl Tetrazolium Bromide

A biosurfactant producing bacterium was identified as Pseudomonas aeruginosa DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-C10, Rha-C10-C12:1, Rha-C16:1, Rha-C16, Rha-C17:1, Rha-Rha-C10:1-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C8, Rha-Rha-C10-C8:1, and Rha-Rha-C8-C8. The radical scavenging activity of rhamnolipid (DNM50RL) was determined by 2, 3-diphenyl-1-picrylhydrazyl (DPPH) assay which showed an IC50 value of 101.8 μg/ ml. The cytotoxic activity was investigated against MDA-MB-231 breast cancer cell line by MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay which showed a very low IC50 of 0.05 μg/ ml at 72 h of treatment. Further, its activity was confirmed by resazurin and trypan blue assay with IC50 values of 0.01 μg/ml and 0.64 μg/ ml at 72 h of treatment, respectively. Thus, the DNM50RL would play a vital role in the treatment of breast cancer targeting inhibition of p38MAPK.

scholarly journals Antioxidant and Anticancer Potential of Methanolic Leaf Extract of Moringa concanensis Nimmo Against Human Breast Cancer Cell Line MCF-7

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
S. Vijayakumar ◽  
V. Bhuvaneshwari ◽  
A. Sumathi
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Plant Sample ◽  
Crude Leaf Extract ◽  
Mcf 7

Breast cancer is one of the major health problems among women in both developed and developing countries. The objective of the present investigation is focused on the DPPH Radical Scavenging Activity and anticancer effect of the methanolic extract of Moringa concanensis Nimmo leaves against Breast cancer (MCF-7) cell line. The study was facilitated by collecting the plant sample and subjected to crude extraction. The anticancer activity of the crude methanolic leaf extract of M. concanensis against MCF- 7 cell line was examined by MTT assay. The present study confirms that the crude leaf extract of M. concanensis has potential anticancer activity against MCF- 7 cell lines while compared to the control. M. concanensis leaves possess remarkable anticancer property which may lead to development of novel compounds as natural phytomedicine.

scholarly journals Antioxidative, Anti-Inflammatory, and Anticancer Effects of Purified Flavonol Glycosides and Aglycones in Green Tea

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 278 ◽  
Author(s):  
Chan-Su Rha ◽  
Hyun Woo Jeong ◽  
Saitbyul Park ◽  
Siyoung Lee ◽  
Young Sung Jung ◽  
...  
Keyword(s):  
Cell Line ◽  
Green Tea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Flavonol Glycosides ◽  
Dependent Manner ◽  
Anticancer Effects ◽  
Anti Inflammatory

(1) Background: Extensive research has focused on flavan-3-ols, but information about the bioactivities of green tea flavonols is limited. (2) Methods: In this study, we investigated the antioxidative, anti-inflammatory, and anticancer effects of flavonol glycosides and aglycones from green tea using in vitro cell models. The fractions rich in flavonol glycoside (FLG) and flavonol aglycone (FLA) were obtained from green tea extract after treatment with tannase and cellulase, respectively. (3) Results: FLG and FLA contained 16 and 13 derivatives, respectively, including apigenin, kaempferol, myricetin, and quercetin, determined by mass spectrometry. FLA exhibited higher radical-scavenging activity than that of FLG. FLG and FLA attenuated the levels of intracellular oxidative stress in neuron-like PC-12 cells. The treatment of RAW 264.7 murine macrophages with FLG and FLA significantly reduced the mRNA expression of inflammation-related genes in a dose-dependent manner. Furthermore, FLG and FLA treatments decreased the viability of the colon adenoma cell line DLD-1 and breast cancer cell line E0771. Moreover, the treatment with FLG or FLA combined with paclitaxel had synergistic anticancer effects on the DLD-1 cell line. (4) Conclusions: Flavonols from green tea exerted beneficial effects on health and may be superior to flavan-3-ols.

Synthesis, Characterization and Biological Evaluation of Indole-Pyrazole Amalgamated α-Cyano Substituted Chalcones

2021 ◽  
Vol 21 ◽  
Author(s):  
Pravin S. Bhale ◽  
Sadanand N. Shringare ◽  
Amol B. Khade ◽  
Hemant V. Chavan
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Safety Margin ◽  
Biological Evaluation ◽  
Biologically Active ◽  
Monkey Cell ◽  
Mcf 7

Background: Indole and pyrazole constitute a major class of biologically active scaffolds. The amalgamation of two or more pharmacophores would generate novel molecular templates that are likely to unveil remarkable biological properties. Objective: An efficient and high yielding synthesis of indole-pyrazole integrated α-cyano substituted chalcones and their in vitro antibreast cancer and antioxidant evaluation. Methods: The synthesis of a series of indole-pyrazole amalgamated α-cyano substituted chalcones (6a-o) was achieved by reacting substituted 3-cyanoacetyl indole 2 with substituted pyrazole aldehyde 5 in the presence of piperidine. All the newly synthesized compounds have been characterized by IR, 1H NMR and HRMS spectroscopy. Results: Anti-breast cancer evaluation of the synthesized compounds in vitro against MCF-7 cell line revealed high anti-breast cancer activities. Amongst the compounds screened 6f, 6g, 6h, 6c, 6d, 6e, 6i and 6k unveiled excellent activity against breast carcinoma (GI50 <0.1µM) as good as adriamycin (GI50 <0.1µM). The compounds were also screened against the normal Vero monkey cell line and the results demonstrated more selectivity against MCF-7. In other hand, compounds, 6b, 6c, 6d, 6h and 6i have shown moderate DPPH and NO radical scavenging activity. Conclusion: Most of the synthesized compounds exhibited significant antitumor activities. These results further support its safety margin by studying the activity on normal Vero monkey cell line. These results acclaim the possible use of these compounds for the design and development of potent anti-breast cancer agents.

Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Jayasri Gunasekaran ◽  
Mirunalini Sankaran
Keyword(s):  
Cell Line ◽  
Mammary Cancer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Scavenging Activity ◽  
Mammary Cancer Cell ◽  
Underlying Mechanisms ◽  
Mcf 7 ◽  
Human Mammary Cancer

Lupeol, a dietary triterpene, present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including anti-cancer activities both in vitro.The present study is conducted to investigate the anticancer activity and the underlying mechanisms of Lupeol analogue (oxime) on human mammary cancer (MCF-7).MTT assay showed that the lupeol analogue had marked cytotoxic activity in MCF-7 cancer cells.We observed increased levels of Antioxidants (ABTS, DPPH,Superoxide anions radical scavenging activity,Hydroxyl radical scavenging activity) in MCF-7 cell line, because of anti oxidant mechanism of lupeol analogue (oxime) .We observed the higher concentrations ( 20,30,40,50 and 70 µM/mL) of lupeol analogue  treatment significantly showed increased cytotoxicity in MCF-7 cells. The IC50 value was found to be 40 µM/mL of lupeol analogue (oxime) could greatly inhibit the cell growth. So, we have chosen at 40 and 320 µM/mL concentration of lupeol analogue (oxime) for further experiments. We observed increased levels of Lipid peroxidation and decreased levels of Antioxidants (SOD,CAT,GPx,GSH) in MCF-7 cell line  because of the pro oxidant mechanism of lupeol analogue (oxime) . These observations revealed the cytotoxic potential of lupeol, which could be attributed to their prooxidant property on the MCF-7 cells. It is evident from the observation made in the present study that the lupeol analogue (oxime)has potential anticancer effect compared to Lupeol.

Biological Evaluation of Curcumin and Related Diarylheptanoids

2006 ◽  
Vol 61 (9-10) ◽  
pp. 625-631 ◽  
Author(s):  
Faridah Abas ◽  
Lim Siang Hui ◽  
Syahida Ahmad ◽  
Johnson Stanslas ◽  
D. A. Israf ◽  
...  
Keyword(s):  
Free Radical ◽  
Cell Line ◽  
Cancer Cell ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Biological Evaluation ◽  
Free Radical Scavenging ◽  
Lung Cancer Cell Line ◽  
Cytotoxic Activities

AbstractNine derivatives of three natural diarylheptanoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin, were prepared. Their antioxidant, free radical scavenging, nitric oxide (NO) inhibitory and cytotoxic activities were evaluated and compared with those of the respective natural compounds. Curcumin (1), demethoxycurcumin (2), demethyldemethoxycurcumin (C3), diacetyldemethoxycurcumin (AC2) and triacetyldemethylcurcumin (AC5) exhibited higher antioxidant activity than quercetin while products from demethylation of 1 and 2 exhibited higher free radical scavenging activity. Compounds AC2 and AC5 were found to be most active in inhibiting breast cancer cells (MCF-7) proliferation with IC50 values of 6.7 and 3.6 μм, respectively. The activity of AC2 is almost doubled and of AC5 almost tripled as compared to curcumin. Their selectivity towards different cell lines is also more noticeable. Compounds AC2 and AC5 also showed increased activity against a human prostate cancer cell line (DU-145) and non-small lung cancer cell line (NCI-H460) with IC50 values of 20.4, 16.3 and 18.3, 10.7 μм, respectively.

scholarly journals ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

2017 ◽  
Vol 9 (2) ◽  
pp. 279 ◽  
Author(s):  
Ahmed R. Abu-raghif ◽  
Ghaith A. Jasim ◽  
Muneer M. Hanoon
Keyword(s):  
Antioxidant Activity ◽  
Cell Line ◽  
Proliferative Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Extraction Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity

<p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p>

ANTIOXIDANT AND ANTI-CANCER ACTVITY OF STANDARDIZED EXTRACTS OF THREE VARIETIES OF Ficus deltoidea’s LEAVES

2015 ◽  
Vol 77 (3) ◽  
Author(s):  
Husnul Hanani Soib ◽  
Ismail Ware ◽  
Harisun Yaakob ◽  
Hanafi Mukrish ◽  
Mohamad Roji Sarmidi
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Total Phenolic ◽  
Ic50 Value ◽  
Anti Cancer

The present study was designed to evaluate the antioxidant and anti-cancer activities of aqueous extracts of three varieties of Ficus deltoidea (var. angustifolia, var. deltoidea, var. kunstleri) on prostate cancer DU145 cell line. The results showed that, F. deltoidea var. kunstleri contained the highest total phenolic (44.7 ± 0.022 mg GAE/10 g sample) and flavonoid contents (23.1 ± 0.005 mg CE/10 g sample) as well as the highest radical scavenging activity (IC50 0.039 mg/mL) followed by F. deltoidea var. deltoidea and F. deltoidea var. angustifolia. Similarly, based on anti-cancer activities, F. deltoidea var. kunstleri demonstrated the lowest IC50 value (93.11 mg/mL) followed by F. deltoidea var. deltoidea (204.17 mg/mL) and F. deltoidea var. angustifolia (429.54 mg/mL). Other than that, vitexin which is a bioactive marker was appeared to be the highest in F. deltoidea var. kunstleri compared to others. Hence, the results suggested that there might be an association between antioxidant activities and bioactive markers against prostate cancer cell line (DU145).

scholarly journals LC-ToF-ESI-MS Patterns of Hirsutinolide-like Sesquiterpenoids Present in the Elephantopus mollis Kunth Extract and Chemophenetic Significance of Its Chemical Constituents

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4810
Author(s):  
Gabin Thierry M. Bitchagno ◽  
Jean Garba Koffi ◽  
Ingrid Konga Simo ◽  
Donald Ulrich K. Kagho ◽  
Augustin Silvere Ngouela ◽  
...  
Keyword(s):  
Cell Line ◽  
Cancer Cell ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Sesquiterpene Lactones ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Flash Chromatography ◽  
Ongoing Research ◽  
Esi Ms

A total of nine sesquiterpenoid lactones together with phenolic compounds and other terpenes were identified from the crude methanol extract of Elephantopus mollis Kunth. Compounds were isolated using different chromatographic techniques and their structures were determined by NMR and IR spectroscopy as well as mass spectrometry. The structures of some detected compounds were assigned based on LC-ToF-ESI-MS screening of main fractions/subfractions from flash chromatography and comparison with isolated analogues as standards. The findings revealed not only the in-source loss of water as the base peak in hirsutinolides but also the in-source loss of corresponding alcohol when the oxygen at position 1 is alkylated. The present study also draws up a complement of data with respect to hirsutinolide-like sesquiterpene lactones whose LC-MS characteristics are not available in the literature. The chemophenetic significance is also discussed. Some of the isolated compounds were reported for the first time to be found in the species, the genus as well as the plant family. The medium-polar fractions of the crude extract, also containing the larger amount of sesquiterpenoid lactones, exhibited activity both against a cancer cell line and bacterial strains. Isolated lactones were also active against the cancer cell line, while the chlorogenic derivatives also valuable in Elephantopus genus showed potent radical scavenging activity. This is the first report of cytotoxic and antibacterial activities of our samples against the tested strains and cell line. The present study follows the ongoing research project dealing with the characterization of taxa with antibacterial and antiparasitic activities from Cameroonian pharmacopeia.

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