scholarly journals A Novel Conductive Antibacterial Nanocomposite Hydrogel Dressing for Healing of Severely Infected Wounds

2021 ◽  
Vol 9 ◽  
Author(s):  
Lizhi Xiao ◽  
Fang Hui ◽  
Tenghui Tian ◽  
Ruyue Yan ◽  
Jingwei Xin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Conductive Polymer ◽  
Antibacterial Properties ◽  
Inhibitory Effect ◽  
Ag Nps ◽  
Host Matrix ◽  
Infected Wounds ◽  
The Matrix ◽  
Infection And Inflammation

Wound infections are serious medical complications that can endanger human health. Latest researches show that conductive composite materials may make endogenous/exogenous electrical stimulation more effective, guide/comb cell migration to the wound, and subsequently promote wound healing. To accelerate infected wound healing, a novel medical silver nanoparticle-doped conductive polymer-based hydrogel system (Ag NPs/CPH) dressing with good conductivity, biocompatibility, and mechanical and antibacterial properties was fabricated. For the hydrogel dressing, Ag NPs/CPH, polyvinyl alcohol (PVA), and gelatin were used as the host matrix materials, and phytic acid (PA) was used as the cross-linking agent to introduce conductive polyaniline into the matrix, with antibacterial Ag NPs loaded via impregnation. After a series of analyses, the material containing 5 wt% of PVA by concentration, 1.5 wt% gelatin, 600 μL of AN reactive volume, and 600 μL of PA reactive volume was chosen for Ag NPs/CPH preparation. XPS and FTIR analysis had been further used to characterize the composition of the prepared Ag NPs/CPH. The test on the swelling property showed that the hydrogels had abundant pores with good water absorption (≈140% within 12 h). They can be loaded and continuously release Ag NPs. Thus, the prepared Ag NPs/CPH showed excellent antibacterial property with increasing duration of immersion of Ag NPs. Additionally, to evaluate in vivo safety, CCK-8 experiments of HaCat, LO2 and 293T cells were treated with different concentrations of the Ag NPs/CPH hydrogel soaking solution. The experimental results showed the Ag NPs/CPH had no significant inhibitory effect on any of the cells. Finally, an innovative infection and inflammation model was designed to evaluate the prepared Ag NPs/CPH hydrogel dressing for the treatment of severely infected wounds. The results showed that even when infected with bacteria for long periods of time (more than 20 h), the proposed conductive antibacterial hydrogel could treat severely infected wounds.

Preparation and evaluation of heparinized sponge based on collagen and chitosan for wound healing

2020 ◽  
Vol 35 (4-5) ◽  
pp. 314-327
Author(s):  
Kun Wu ◽  
Dan Zhao ◽  
Huifei Cui
Keyword(s):  
Wound Healing ◽  
Antibacterial Properties ◽  
Protective Role ◽  
Effect Analysis ◽  
Enhanced Expression ◽  
Infection And Inflammation ◽  
Infrared Spectrometer ◽  
Skin Healing

The wound dressing can temporarily replace the skin and play a protective role in the process of wound healing, preventing wound infection and inflammation, and providing a favorable environment for wound healing. In this study, a mixture of collagen and chitosan was lyophilized to be the host material of the sponge. This sponge was soaked into 1-ethyl-(dimethylaminopropyl) carbodiimide/N-hydroxy sulfosuccinimide cross-linking solution containing heparin and experienced secondary lyophilization to prepare the heparinized sponge (CT-CL/Hp). The surface morphology and structural characterization of the sponge was characterized by scanning electron microscope and Fourier transform infrared spectrometer, respectively. Relatively favorable water absorption capability were observed by measuring the physical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The sustained-release property of heparin from the sponges was measured using Alcian Blue assay. Experiments in vitro and in vivo showed that the sponges had satisfactory biocompatibility and lower sensitization. Moreover, the effect of sponge on early stages of wound healing was evaluated by guinea pigs wound healing models. Analysis of wound healing rates and histological examination showed satisfactory results. CT-CL/Hp enhanced expression of growth factors, particularly VEGF and EGF at day 7. These results demonstrated that CT-CL/Hp–treated sponges benefit to wound skin healing and regeneration.

Preparation of biocompatible wound dressings with dual release of antibiotic and platelet-rich plasma for enhancing infected wound healing

2021 ◽  
pp. 088532822199601
Author(s):  
Linying Shi ◽  
Fang Lin ◽  
Mou Zhou ◽  
Yanhui Li ◽  
Wendan Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Wound Dressings ◽  
Inflammatory Factors ◽  
Gelatin Microspheres ◽  
Infected Wounds ◽  
Dual Release

The ever-growing threats of bacterial infection and chronic wound healing have provoked an urgent need for novel antibacterial wound dressings. In this study, we developed a wound dressing for the treatment of infected wounds, which can reduce the inflammatory period (through the use of gentamycin sulfate (GS)) and enhance the granulation stage (through the addition of platelet-rich plasma (PRP)). Herein, the sustained antimicrobial CMC/GMs@GS/PRP wound dressings were developed by using gelatin microspheres (GMs) loading GS and PRP, covalent bonding to carboxymethyl chitosan (CMC). The prepared dressings exhibited high water uptake capability, appropriate porosity, excellent mechanical properties, sustain release of PRP and GS. Meanwhile, the wound dressing showed good biocompatibility and excellent antibacterial ability against Gram-negative and Gram-positive bacteria. Moreover, in vivo experiments further demonstrated that the prepared dressings could accelerate the healing process of E. coli and S. aureus-infected full-thickness wounds i n vivo, reepithelialization, collagen deposition and angiogenesis. In addition, the treatment of CMC/GMs@GS/PRP wound dressing could reduce bacterial count, inhibit pro-inflammatory factors (TNF-α, IL-1β and IL-6), and enhance anti-inflammatory factors (TGF-β1). The findings of this study suggested that biocompatible wound dressings with dual release of GS and PRP have great potential in the treatment of chronic and infected wounds.

scholarly journals Antimicrobial and anti-biofilm potencies of dermcidin-derived peptide DCD-1L against Acinetobacter baumannii: an in vivo wound healing model

2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Zahra Farshadzadeh ◽  
Maryam Pourhajibagher ◽  
Behrouz Taheri ◽  
Alireza Ekrami ◽  
Mohammad Hossein Modarressi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Histopathological Examination ◽  
Inhibitory Effect ◽  
Bacterial Attachment ◽  
Burn Wound ◽  
Conventional Antibiotics

Abstract Background The global emergence of Acinetobacter baumannii resistance to most conventional antibiotics presents a major therapeutic challenge and necessitates the discovery of new antibacterial agents. The purpose of this study was to investigate in vitro and in vivo anti-biofilm potency of dermcidin-1L (DCD-1L) against extensively drug-resistant (XDR)-, pandrug-resistant (PDR)-, and ATCC19606-A. baumannii. Methods After determination of minimum inhibitory concentration (MIC) of DCD-1L, in vitro anti-adhesive and anti-biofilm activities of DCD-1L were evaluated. Cytotoxicity, hemolytic activity, and the effect of DCD-1L treatment on the expression of various biofilm-associated genes were determined. The inhibitory effect of DCD-1L on biofilm formation in the model of catheter-associated infection, as well as, histopathological examination of the burn wound sites of mice treated with DCD-1L were assessed. Results The bacterial adhesion and biofilm formation in all A. baumannii isolates were inhibited at 2 × , 4 × , and 8 × MIC of DCD-1L, while only 8 × MIC of DCD-1L was able to destroy the pre-formed biofilm in vitro. Also, reduce the expression of genes involved in biofilm formation was observed following DCD-1L treatment. DCD-1L without cytotoxic and hemolytic activities significantly reduced the biofilm formation in the model of catheter-associated infection. In vivo results showed that the count of A. baumannii in infected wounds was significantly decreased and the promotion in wound healing by the acceleration of skin re-epithelialization in mice was observed following treatment with 8 × MIC of DCD-1L. Conclusions Results of this study demonstrated that DCD-1L can inhibit bacterial attachment and biofilm formation and prevent the onset of infection. Taking these properties together, DCD-1L appears as a promising candidate for antimicrobial and anti-biofilm drug development.

scholarly journals CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling

2007 ◽  
Vol 178 (4) ◽  
pp. 701-711 ◽  
Author(s):  
Chloé C. Féral ◽  
Andries Zijlstra ◽  
Eugene Tkachenko ◽  
Gerald Prager ◽  
Margaret L. Gardel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Membrane Protein ◽  
Small Gtpase ◽  
Integrin Signaling ◽  
Vertebrate Development ◽  
Matrix Assembly ◽  
Fibronectin Matrix ◽  
The Matrix

Integrin-dependent assembly of the fibronectin (Fn) matrix plays a central role in vertebrate development. We identify CD98hc, a membrane protein, as an important component of the matrix assembly machinery both in vitro and in vivo. CD98hc was not required for biosynthesis of cellular Fn or the maintenance of the repertoire or affinity of cellular Fn binding integrins, which are important contributors to Fn assembly. Instead, CD98hc was involved in the cell's ability to exert force on the matrix and did so by dint of its capacity to interact with integrins to support downstream signals that lead to activation of RhoA small GTPase. Thus, we identify CD98hc as a membrane protein that enables matrix assembly and establish that it functions by interacting with integrins to support RhoA-driven contractility. CD98hc expression can vary widely; our data show that these variations in CD98hc expression can control the capacity of cells to assemble an Fn matrix, a process important in development, wound healing, and tumorigenesis.

scholarly journals Eradication of Mature Bacterial Biofilms with Concurrent Improvement in Chronic Wound Healing Using Silver Nanoparticle Hydrogel Treatment

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1182
Author(s):  
Hanif Haidari ◽  
Richard Bright ◽  
Sanjay Garg ◽  
Krasimir Vasilev ◽  
Allison J. Cowin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Wound Closure ◽  
In Vivo Studies ◽  
Release Mechanism ◽  
Impaired Wound Healing ◽  
Biofilm Model ◽  
Infected Wounds ◽  
Delivery Platform

Biofilm-associated infections are a major cause of impaired wound healing. Despite the broad spectrum of anti-bacterial benefits provided by silver nanoparticles (AgNPs), these materials still cause controversy due to cytotoxicity and a lack of efficacy against mature biofilms. Herein, highly potent ultrasmall AgNPs were combined with a biocompatible hydrogel with integrated synergistic functionalities to facilitate elimination of clinically relevant mature biofilms in-vivo combined with improved wound healing capacity. The delivery platform showed a superior release mechanism, reflected by high biocompatibility, hemocompatibility, and extended antibacterial efficacy. In vivo studies using the S. aureus wound biofilm model showed that the AgNP hydrogel (200 µg/g) was highly effective in eliminating biofilm infection and promoting wound repair compared to the controls, including silver sulfadiazine (Ag SD). Treatment of infected wounds with the AgNP hydrogel resulted in faster wound closure (46% closure compared to 20% for Ag SD) and accelerated wound re-epithelization (60% for AgNP), as well as improved early collagen deposition. The AgNP hydrogel did not show any toxicity to tissue and/or organs. These findings suggest that the developed AgNP hydrogel has the potential to be a safe wound treatment capable of eliminating infection and providing a safe yet effective strategy for the treatment of infected wounds.

scholarly journals Antibacterial Ferroelectric Hybrid Membranes Fabricated via Electrospinning for Wound Healing

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 986
Author(s):  
Ivan V. Lukiev ◽  
Ludmila S. Antipina ◽  
Semen I. Goreninskii ◽  
Tamara S. Tverdokhlebova ◽  
Dmitry V. Vasilchenko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Vinylidene Fluoride ◽  
Antibacterial Properties ◽  
Oxide Nanoparticles ◽  
Hybrid Membranes ◽  
Electrospinning Technique ◽  
Β Phase

In the present study, wound healing ferroelectric membranes doped with zinc oxide nanoparticles were fabricated from vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone using the electrospinning technique. Five different ratios of vinylidene fluoride-tetrafluoroethylene to polyvinylpyrrolidone were used to control the properties of the membranes at a constant zinc oxide nanoparticle content. It was found that an increase of polyvinylpyrrolidone content leads to a decrease of the spinning solution conductivity and viscosity, causing a decrease of the average fiber diameter and reducing their strength and elongation. By means of X-ray diffraction and infrared spectroscopy, it was revealed that increased polyvinylpyrrolidone content leads to difficulty in crystallization of the vinylidene fluoride-tetrafluoroethylene copolymer in the ferroelectric β-phase in membranes. Changing the ratio of vinylidene fluoride-tetrafluoroethylene copolymer and polyvinylpyrrolidone with a constant content of zinc oxide nanoparticles is an effective approach to control the antibacterial properties of membranes towards Staphylococcus aureus. After carrying out in vivo experiments, we found that ferroelectric hybrid membranes, containing from five to ten mass percent of PVP, have the greatest wound-healing effect for the healing of purulent wounds.

scholarly journals Integrated endotoxin-adsorption and antibacterial properties of platelet-membrane-coated copper silicate hollow microspheres for wound healing

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Zaihui Peng ◽  
Xiaochun Zhang ◽  
Long Yuan ◽  
Ting Li ◽  
Yajie Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bacterial Infections ◽  
Antibacterial Properties ◽  
Platelet Membrane ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
The Impact ◽  
Copper Silicate

AbstractSerious infection caused by drug-resistant gram-negative bacteria and their secreted toxins (e.g., lipopolysaccharide) is a serious threat to human health. Thus, treatment strategies that efficiently kill bacteria and reducing the impact of their toxins simultaneously are urgently required. Herein, a novel antibacterial platform composed of a mesoporous copper silicate microsphere (CSO) core and a platelet membrane (PM) shell was prepared (CSO@PM). CSO@PM specifically targets bacteria owing to formyl peptide receptors on the PM and, combined with photothermal therapy (PTT), exhibits highly effective bacter icidal activity. Importantly, CSO@PM can adsorb lipopolysaccharide secreted by gram-negative bacteria, resulting in inflammation reduction. Thus, CSO@PM stimulates re-epithelialization and granulation-tissue formation, promoting wound healing. Moreover, this antibacterial platform exhibits no obvious toxicity at all the test concentrations in vitro and in vivo. Thus, CSO@PM exhibits a robust antibacterial effect and a strong toxin-adsorption capacity, facilitating the clinical treatment of many bacterial infections and the development of next-generation antibacterial nanoagents. Graphical Abstract

Alginate/Gelatin Sponges Composited with ZnO Sponge Effective Extensibility and Compressibility as a Wound Dressing for the Care of Fracture Surgery

2021 ◽  
Vol 11 (10) ◽  
pp. 1873-1880
Author(s):  
Yumei Wang ◽  
Huichao Fu ◽  
Ying Lin
Keyword(s):  
Wound Healing ◽  
Human Fibroblasts ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Surface Characteristics ◽  
Future Application ◽  
Ft Ir ◽  
Cell Construction ◽  
Ft Ir Spectroscopy

We designed and synthesized highly porous alginate and gelatin hydrogels with zinc oxide nanoparticles (AGZNPs) as dressings using sol–gel methods. The presence of functional groups and the surface characteristics of the as-synthesised dressings were analyzed via Fourier transforminfrared (FT-IR) spectroscopy, while their morphology was studied via scanning electron microscopy (SEM). Additionally, the mechanical, inflammatory, and antibacterial properties and biocompatibility of the AGZNPs were evaluated to determine the efficiency of these bandages for wound healing applications. The AGZNPs demonstrated enhanced inflammatory and antibacterial properties. The biocompatibility of the nanocomposites was investigated in noncancerous NIH3T3 human fibroblasts. Furthermore, in-vivo examinations showed that the composition of the synthesized AGZNPs enhanced wound healing and promoted rapid cell construction and growth. Therefore, the AGZNP strategy promotes the future application of these nanoformulation hydrogels for wound bandaging in fracture surgeries.

In vivo wound healing efficiency of curcumin-incorporated pectin-chitosan biodegradable films

2021 ◽  
Author(s):  
V. Muthulakshmi ◽  
G. R. Rajarajeswari
Keyword(s):  
Thin Films ◽  
Wound Healing ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
Biodegradable Films ◽  
Healing Efficiency ◽  
Mechanical Barrier

Curcumin incorporated pectin/chitosan thin films application as a potential wound dressing material with good mechanical, barrier and antibacterial properties.

scholarly journals Copper/Zinc-Modified Palygorskite Protects Against Salmonella Typhimurium Infection and Modulates the Intestinal Microbiota in Chickens

2021 ◽  
Vol 12 ◽  
Author(s):  
Chaozheng Zhang ◽  
Dawei Yao ◽  
Zenan Su ◽  
Huan Chen ◽  
Pan Hao ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Intestinal Inflammation ◽  
Antibacterial Properties ◽  
Inhibitory Effect ◽  
Mucosal Barrier ◽  
Serovar Typhimurium ◽  
Copper Zinc ◽  
Modified Palygorskite

Palygorskite (Pal), a clay nanoparticle, has been demonstrated to be a vehicle for drug delivery. Copper has antibacterial properties, and zinc is an essential micronutrient for intestinal health in animals and humans. However, whether copper/zinc-modified Pal (Cu/Zn-Pal) can protect chickens from Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) infection remains unclear. In this study, three complexes (Cu/Zn-Pal-1, Cu/Zn-Pal-2, and Cu/Zn-Pal-3) were prepared, and Cu/Zn-Pal-1 was shown to be the most effective at inhibiting the growth of S. Typhimurium in vitro, whereas natural Pal alone had no inhibitory effect. In vivo, Cu/Zn-Pal-1 reduced S. Typhimurium colonization in the intestine of infected chickens and relieved S. Typhimurium-induced organ and intestinal mucosal barrier damage. Moreover, this reduction in Salmonella load attenuated intestinal inflammation and the oxidative stress response in challenged chickens. Additionally, Cu/Zn-Pal-1 modulated the intestinal microbiota in infected chickens, which was characterized by the reduced abundance of Firmicutes and the increased abundance of Proteobacteria and Bacteroidetes. Our results indicated that the Cu/Zn-Pal-1 complex may be an effective feed supplement for reducing S. Typhimurium colonization of the gut.

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