scholarly journals Microbial Signatures in Deep CO2-Saturated Miocene Sediments of the Active Hartoušov Mofette System (NW Czech Republic)

2020 ◽  
Vol 11 ◽  
Author(s):  
Qi Liu ◽  
Karsten Adler ◽  
Daniel Lipus ◽  
Horst Kämpf ◽  
Robert Bussert ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Dna Analysis ◽  
Quantitative Polymerase Chain Reaction ◽  
Lacustrine Sediments ◽  
Deep Biosphere ◽  
Upward Migration ◽  
Eger Rift ◽  
And Migration ◽  
Cheb Basin ◽  
The Impact

The Hartoušov mofette system is a natural CO2 degassing site in the central Cheb Basin (Eger Rift, Central Europe). In early 2016 a 108 m deep core was obtained from this system to investigate the impact of ascending mantle-derived CO2 on indigenous deep microbial communities and their surrounding life habitat. During drilling, a CO2 blow out occurred at a depth of 78.5 meter below surface (mbs) suggesting a CO2 reservoir associated with a deep low-permeable CO2-saturated saline aquifer at the transition from Early Miocene terrestrial to lacustrine sediments. Past microbial communities were investigated by hopanoids and glycerol dialkyl glycerol tetraethers (GDGTs) reflecting the environmental conditions during the time of deposition rather than showing a signal of the current deep biosphere. The composition and distribution of the deep microbial community potentially stimulated by the upward migration of CO2 starting during Mid Pleistocene time was investigated by intact polar lipids (IPLs), quantitative polymerase chain reaction (qPCR), and deoxyribonucleic acid (DNA) analysis. The deep biosphere is characterized by microorganisms that are linked to the distribution and migration of the ascending CO2-saturated groundwater and the availability of organic matter instead of being linked to single lithological units of the investigated rock profile. Our findings revealed high relative abundances of common soil and water bacteria, in particular the facultative, anaerobic and potential iron-oxidizing Acidovorax and other members of the family Comamonadaceae across the whole recovered core. The results also highlighted the frequent detection of the putative sulfate-oxidizing and CO2-fixating genus Sulfuricurvum at certain depths. A set of new IPLs are suggested to be indicative for microorganisms associated to CO2 accumulation in the mofette system.

scholarly journals Weak Influence of Paleoenvironmental Conditions on the Subsurface Biosphere of Lake Ohrid over the Last 515 ka

2020 ◽  
Vol 8 (11) ◽  
pp. 1736
Author(s):  
Camille Thomas ◽  
Alexander Francke ◽  
Hendrik Vogel ◽  
Bernd Wagner ◽  
Daniel Ariztegui
Keyword(s):  
Microbial Communities ◽  
Lacustrine Sediments ◽  
Environmental Parameters ◽  
Strong Relationship ◽  
Low Energy ◽  
Rrna Gene ◽  
Deep Biosphere ◽  
Lake Ohrid ◽  
Metabolic Versatility ◽  
The Impact

Lacustrine sediments are widely used to investigate the impact of climatic change on biogeochemical cycling. In these sediments, subsurface microbial communities are major actors of this cycling but can also affect the sedimentary record and overprint the original paleoenvironmental signal. We therefore investigated the subsurface microbial communities of the oldest lake in Europe, Lake Ohrid (North Macedonia, Albania), to assess the potential connection between microbial diversity and past environmental change using 16S rRNA gene sequences. Along the upper ca. 200 m of the DEEP site sediment record spanning ca. 515 thousand years (ka), our results show that Atribacteria, Bathyarchaeia and Gammaproteobacteria structured the community independently from each other. Except for the latter, these taxa are common in deep lacustrine and marine sediments due to their metabolic versatility adapted to low energy environments. Gammaproteobacteria were often co-occurring with cyanobacterial sequences or soil-related OTUs suggesting preservation of ancient DNA from the water column or catchment back to at least 340 ka, particularly in dry glacial intervals. We found significant environmental parameters influencing the overall microbial community distribution, but no strong relationship with given phylotypes and paleoclimatic signals or sediment age. Our results support a weak recording of early diagenetic processes and their actors by bulk prokaryotic sedimentary DNA in Lake Ohrid, replaced by specialized low-energy clades of the deep biosphere and a marked imprint of erosional processes on the subsurface DNA pool of Lake Ohrid.

scholarly journals Shaping of the Present-Day Deep Biosphere at Chicxulub by the Impact Catastrophe That Ended the Cretaceous

2021 ◽  
Vol 12 ◽  
Author(s):  
Charles S. Cockell ◽  
Bettina Schaefer ◽  
Cornelia Wuchter ◽  
Marco J. L. Coolen ◽  
Kliti Grice ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Amplicon Sequencing ◽  
Granitic Rocks ◽  
Rrna Gene ◽  
Deep Biosphere ◽  
Deep Subsurface ◽  
Cell Biomass ◽  
Colonization Potential ◽  
The Impact

We report on the effect of the end-Cretaceous impact event on the present-day deep microbial biosphere at the impact site. IODP-ICDP Expedition 364 drilled into the peak ring of the Chicxulub crater, México, allowing us to investigate the microbial communities within this structure. Increased cell biomass was found in the impact suevite, which was deposited within the first few hours of the Cenozoic, demonstrating that the impact produced a new lithological horizon that caused a long-term improvement in deep subsurface colonization potential. In the biologically impoverished granitic rocks, we observed increased cell abundances at impact-induced geological interfaces, that can be attributed to the nutritionally diverse substrates and/or elevated fluid flow. 16S rRNA gene amplicon sequencing revealed taxonomically distinct microbial communities in each crater lithology. These observations show that the impact caused geological deformation that continues to shape the deep subsurface biosphere at Chicxulub in the present day.

scholarly journals Deciphering the Influence of Multiple Anthropogenic Inputs on Taxonomic and Functional Profiles of the Microbial Communities in Yitong River, Northeast China

2021 ◽  
Author(s):  
Ying Zhang ◽  
Yang Huo ◽  
Zhiruo Zhang ◽  
Suiyi Zhu ◽  
Wei Fan ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Northeast China ◽  
Environmental Changes ◽  
Quantitative Polymerase Chain Reaction ◽  
Amplicon Sequencing ◽  
Fecal Indicator ◽  
16S Rrna Amplicon Sequencing ◽  
Anthropogenic Inputs ◽  
Wwtp Effluent ◽  
The Impact

Abstract We conducted physicochemical parameters analysis, 16S rRNA amplicon sequencing and real-time quantitative polymerase chain reaction to explore the impact of human inputs on the bacterioplankton communities within a tributary of the largest river flowing through a megacity in northeast China. Agriculture largely accounted for the alteration of diversity and functions of the microbial communities. Furthermore, nutrients were significantly declined at the reservoir outlet, and WWTP effluent discharge caused changes in the river microbial community. NH3-N and NO3--N were the main environmental factors that drive the shift of the bacteria community, and rare taxa played a more important role in the response to environmental changes compared with the abundant ones. The occurrence of the human-specific fecal indicator was mostly derived from agriculture, and its increase in relative abundance was observed in the effluent. Thus, our study provides guidance for ecological assessment and management of rivers by revealing the response pattern of river bacterioplankton to multiple types of anthropogenic stressors.

Variations of gas compositions during a drilling process: A key study on the Hartoušov Mofette, Czech Republic

2020 ◽  
Author(s):  
Kyriaki Daskalopoulou ◽  
Heiko Woith ◽  
Martin Zimmer ◽  
Samuel Niedermann ◽  
Cemile D. Bağ ◽  
...  
Keyword(s):  
Czech Republic ◽  
Isotope Composition ◽  
Earthquake Swarms ◽  
Earthquake Activity ◽  
Drilling Process ◽  
Deep Biosphere ◽  
Eger Rift ◽  
Environmental Air ◽  
A Minor ◽  
Cheb Basin

<p>The Eger Rift (Czech Republic) is an intraplate region without active volcanism but with emanations of magma-derived gases and the recurrence of mid-crustal earthquake swarms with small to intermediate magnitudes (M<sub>L</sub> < 5) in the Cheb Basin. To understand the anomalous earthquake activity and CO<sub>2</sub> degassing, an interdisciplinary well-based observatory is built up for continuous fluid and earthquake monitoring at depth.</p><p>The fluid observatory is located at the Hartoušov Mofette (Cheb Basin), an area characterized by intense mantle degassing with a subcontinental lithospheric mantle (SCLM) contribution of He that increased from 38% in 1993 to 89% in 2016. Two drillings with depths of 30 and 108 m (F1 and F2, respectively) are being monitored since August 2019 for the composition of ascending fluids. Additionally, the environmental air composition is monitored. Gas concentrations were determined in-situ at 1-min intervals, while direct sampling campaigns took place periodically and samples were analyzed for their chemical and isotope composition. Samples of gases emerging in the mofette were also collected. During this period, a third borehole (F3) with a depth of 238 m was drilled.</p><p>At Hartoušov, carbon dioxide is the prevailing gas component (concentrations above 99.5%), with helium presenting a mantle origin (up to 90% considering a SCLM-type source). The atmospheric contribution is negligible, even though during drilling of F3 enrichments in atmospheric components such as Ar and N<sub>2</sub> have been observed. An increase in both CH<sub>4</sub> and He has been noticed in F2 (108 m borehole) at 40 m depth, whilst a decrease in He has been observed at 193 m depth in both F1 and the natural mofette. Enrichments in less soluble gases (eg. He and N<sub>2</sub>) at various depths accompanied by a minor CO<sub>2</sub> decrease have also been noticed. Such variations may have been caused by the different solubilities of gases in aquatic environments. Moreover, a decrease in CO<sub>2</sub> followed by a subsequent enrichment of CH<sub>4</sub> and C<sub>x</sub>H<sub>y</sub> during the first days after the initial drilling could promote the hypothesis of the generation of microbialy derived CH<sub>4</sub>. Diurnal variations were observed for the majority of the gas components during the last phase of the F3 drilling, when the well reached a depth >200 m.</p><p>This research is a part of the MoRe - “Mofette Research” project, which is included in the ICDP project “Drilling the Eger Rift: Magmatic fluids driving the earthquake swarms and the deep biosphere”). This work was supported by the DFG grant# WO 855/4-1 and BA 2207/19-1.</p>

scholarly journals Drilling into an active mofette: pilot-hole study of the impact of CO<sub>2</sub>-rich mantle-derived fluids on the geo–bio interaction in the western Eger Rift (Czech Republic)

2017 ◽  
Vol 23 ◽  
pp. 13-27 ◽  
Author(s):  
Robert Bussert ◽  
Horst Kämpf ◽  
Christina Flechsig ◽  
Katja Hesse ◽  
Tobias Nickschick ◽  
...  
Keyword(s):  
Czech Republic ◽  
Active Faults ◽  
Pumping Test ◽  
Mineral Waters ◽  
Deep Biosphere ◽  
Pilot Hole ◽  
Microbial Life ◽  
Core Recovery ◽  
Eger Rift ◽  
The Impact

Abstract. Microbial life in the continental deep biosphere is closely linked to geodynamic processes, yet this interaction is poorly studied. The Cheb Basin in the western Eger Rift (Czech Republic) is an ideal place for such a study because it displays almost permanent seismic activity along active faults with earthquake swarms up to ML 4.5 and intense degassing of mantle-derived CO2 in conduits that show up at the surface in form of mofettes. We hypothesize that microbial life is significantly accelerated in active fault zones and in CO2 conduits, due to increased fluid and substrate flow. To test this hypothesis, pilot hole HJB-1 was drilled in spring 2016 at the major mofette of the Hartoušov mofette field, after extensive pre-drill surveys to optimize the well location. After drilling through a thin caprock-like structure at 78.5 m, a CO2 blowout occurred indicating a CO2 reservoir in the underlying sandy clay. A pumping test revealed the presence of mineral water dominated by Na+, Ca2+, HCO3−, SO42− (Na-Ca-HCO3-SO4 type) having a temperature of 18.6 °C and a conductivity of 6760 µS cm−1. The high content of sulfate (1470 mg L−1) is typical of Carlsbad Spa mineral waters. The hole penetrated about 90 m of Cenozoic sediments and reached a final depth of 108.50 m in Palaeozoic schists. Core recovery was about 85 %. The cored sediments are mudstones with minor carbonates, sandstones and lignite coals that were deposited in a lacustrine environment. Deformation structures and alteration features are abundant in the core. Ongoing studies will show if they result from the flow of CO2-rich fluids or not.

Syrian Crisis and Migration

2015 ◽  
Vol 12 (3) ◽  
pp. 181-192 ◽  
Author(s):  
Pinar Yazgan ◽  
Deniz Eroglu Utku ◽  
Ibrahim Sirkeci
Keyword(s):  
Human Mobility ◽  
Special Issue ◽  
Host Countries ◽  
Syrian Crisis ◽  
Mass Migration ◽  
Large Numbers ◽  
And Migration ◽  
Europe Today ◽  
The Impact ◽  
Near Future

With the growing insurrections in Syria in 2011, an exodus in large numbers have emerged. The turmoil and violence have caused mass migration to destinations both within the region and beyond. The current "refugee crisis" has escalated sharply and its impact is widening from neighbouring countries toward Europe. Today, the Syrian crisis is the major cause for an increase in displacement and the resultant dire humanitarian situation in the region. Since the conflict shows no signs of abating in the near future, there is a constant increase in the number of Syrians fleeing their homes. However, questions on the future impact of the Syrian crisis on the scope and scale of this human mobility are still to be answered. As the impact of the Syrian crisis on host countries increases, so does the demand for the analyses of the needs for development and protection in these countries. In this special issue, we aim to bring together a number of studies examining and discussing human mobility in relation to the Syrian crisis.

Race, Gender, and Border Control in the Western Balkans

2018 ◽  
Author(s):  
Sanja Milivojević
Keyword(s):  
Knowledge Production ◽  
Asylum Seekers ◽  
Former Yugoslavia ◽  
Border Control ◽  
Western Balkans ◽  
Irregular Migrants ◽  
Criminological Inquiry ◽  
Gender And Migration ◽  
And Migration ◽  
The Impact

This chapter looks at the intersection of race, gender, and migration in the Western Balkans. Immobilizing mobile bodies from the Global South has increasingly been the focus of criminological inquiry. Such inquiry, however, has largely excluded the Western Balkans. A difficult place to research, comprising countries of the former Yugoslavia and Albania, the region is the second-largest route for irregular migrants in Europe (Frontex 2016). Indeed, EU expansion and global developments such as wars in Syria, Afghanistan, and Iraq have had a major impact on mobility and migration in the region. The chapter outlines racialized hierarchies in play in contemporary border policing in the region, and how these racialized and gendered practices target racially different Others and women irregular migrants and asylum seekers. Finally, this chapter maps the impact of such practices and calls for a shift in knowledge production in documenting and addressing such discriminatory practices.

scholarly journals Does Emancipation Matter? Fertility of Chinese International Migrants to the United States and Nonmigrants during China’s One-child Policy Period

2021 ◽  
pp. 019791832199478
Author(s):  
Wanli Nie ◽  
Pau Baizan
Keyword(s):  
United States ◽  
International Migration ◽  
Chinese Women ◽  
The United States ◽  
Selection Effects ◽  
Birth Cohorts ◽  
Family Policies ◽  
Chinese Migrants ◽  
And Migration ◽  
The Impact

This article investigates the impact of international migration to the United States on the level and timing of Chinese migrants’ fertility. We compare Chinese women who did not leave the country (non-migrants) and were subject to restrictive family policies from 1974 to 2015 to those who moved to the United States (migrants) and were, thus, “emancipated” from these policies. We theoretically develop and empirically test the emancipation hypothesis that migrants should have a higher fertility than non-migrants, as well as an earlier timing of childbearing. This emancipation effect is hypothesized to decline across birth cohorts. We use data from the 2000 US census, the 2005 American Community Survey, the 2000 Chinese census, and the 2005 Chinese 1 percent Population Survey and discrete-time event history models to analyze first, second, and third births, and migration as joint processes, to account for selection effects. The results show that Chinese migrants to the United States had substantially higher childbearing probabilities after migration, compared with non-migrants in China, especially for second and third births. Moreover, our analyses indicate that the migration process is selective of migrants with lower fertility. Overall, the results show how international migration from China to the United States can lead to an increase in migrant women’s fertility, accounting for disruption, adaptation, and selection effects. The rapidly increased fertility after migration from China to the United States might have implications on other migration contexts where fertility in the origin country is dropping rapidly while that in the destination country is relatively stable.

scholarly journals Benchmarking microbiome transformations favors experimental quantitative approaches to address compositionality and sampling depth biases

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Verónica Lloréns-Rico ◽  
Sara Vieira-Silva ◽  
Pedro J. Gonçalves ◽  
Gwen Falony ◽  
Jeroen Raes
Keyword(s):  
Microbial Communities ◽  
Quantitative Methods ◽  
Microbial Load ◽  
Metagenomic Sequencing ◽  
Sampling Depth ◽  
Microbiome Research ◽  
Microbiome Data ◽  
The Impact ◽  
Analytical Approaches ◽  
Quantitative Approaches

AbstractWhile metagenomic sequencing has become the tool of preference to study host-associated microbial communities, downstream analyses and clinical interpretation of microbiome data remains challenging due to the sparsity and compositionality of sequence matrices. Here, we evaluate both computational and experimental approaches proposed to mitigate the impact of these outstanding issues. Generating fecal metagenomes drawn from simulated microbial communities, we benchmark the performance of thirteen commonly used analytical approaches in terms of diversity estimation, identification of taxon-taxon associations, and assessment of taxon-metadata correlations under the challenge of varying microbial ecosystem loads. We find quantitative approaches including experimental procedures to incorporate microbial load variation in downstream analyses to perform significantly better than computational strategies designed to mitigate data compositionality and sparsity, not only improving the identification of true positive associations, but also reducing false positive detection. When analyzing simulated scenarios of low microbial load dysbiosis as observed in inflammatory pathologies, quantitative methods correcting for sampling depth show higher precision compared to uncorrected scaling. Overall, our findings advocate for a wider adoption of experimental quantitative approaches in microbiome research, yet also suggest preferred transformations for specific cases where determination of microbial load of samples is not feasible.

scholarly journals The DEAD/DEAH Box Helicase, DDX11, Is Essential for the Survival of Advanced Clear Cell Renal Cell Carcinoma and Is a Determinant of PARP Inhibitor Sensitivity

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2574
Author(s):  
Jee Soo Park ◽  
Myung Eun Lee ◽  
Won Sik Jang ◽  
Koon Ho Rha ◽  
Seung Hwan Lee ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Quantitative Polymerase Chain Reaction ◽  
Parp Inhibitor ◽  
Renal Tumors ◽  
Low Grade ◽  
Normal Kidney ◽  
The Dead ◽  
The Impact

Genes associated with the DEAD-box helicase DDX11 are significant biomarkers of aggressive renal cell carcinoma (RCC), but their molecular function is poorly understood. We analyzed the molecular pathways through which DDX11 is involved in RCC cell survival and poly (ADP-ribose) polymerase (PARP) inhibitor sensitivity. Immunohistochemistry and immunoblotting determined DDX11 expression in normal kidney tissues, benign renal tumors, and RCC tissues and cell lines. Quantitative polymerase chain reaction validated the downregulation of DDX11 in response to transfection with DDX11-specific small interfering RNA. Proliferation analysis and apoptosis assays were performed to determine the impact of DDX11 knockdown on RCC cells, and the relevant effects of sunitinib, olaparib, and sunitinib plus olaparib were evaluated. DDX11 was upregulated in high-grade, advanced RCC compared to low-grade, localized RCC, and DDX11 was not expressed in normal kidney tissues or benign renal tumors. DDX11 knockdown resulted in the inhibition of RCC cell proliferation, segregation defects, and rapid apoptosis. DDX11-deficient RCC cells exhibited significantly increased sensitivity to olaparib compared to sunitinib alone or sunitinib plus olaparib combination treatments. Moreover, DDX11 could determine PARP inhibitor sensitivity in RCC. DDX11 could serve as a novel therapeutic biomarker for RCC patients who are refractory to conventional targeted therapies and immunotherapies.

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