scholarly journals Impact of Tellurite on the Metabolism of Paenibacillus pabuli AL109b With Flagellin Production Explaining High Reduction Capacity

2021 ◽  
Vol 12 ◽  
Pedro Farias ◽  
Romeu Francisco ◽  
Lorrie Maccario ◽  
Jakob Herschend ◽  
Ana Paula Piedade ◽  

Tellurium (Te) is a metalloid with scarce and scattered abundance but with an increased interest in human activity for its uses in emerging technologies. As is seen for other metals and metalloids, the result of mining activity and improper disposal of high-tech devices will lead to niches with increased abundance of Te. This metalloid will be more available to bacteria and represent an increasing selective pressure. This environmental problem may constitute an opportunity to search for microorganisms with genetic and molecular mechanisms of microbial resistance to Te toxic anions. Organisms from Te-contaminated niches could provide tools for Te remediation and fabrication of Te-containing structures with added value. The objective of this study was to determine the ability of a high metal-resistant Paenibacillus pabuli strain ALJ109b, isolated from high metal content mining residues, to reduce tellurite ion, and to evaluate the formation of metallic tellurium by cellular reduction, isolate the protein responsible, and determine the metabolic response to tellurite during growth. P. pabuli ALJ109b demonstrated to be resistant to Te (IV) at concentrations higher than reported for its genus. It can efficiently remove soluble Te (IV) from solution, over 20% in 8 h of growth, and reduce it to elemental Te, forming monodisperse nanostructures, verified by scattering electron microscopy. Cultivation of P. pabuli ALJ109b in the presence of Te (IV) affected the general protein expression pattern, and hence the metabolism, as demonstrated by high-throughput proteomic analysis. The Te (IV)-induced metabolic shift is characterized by an activation of ROS response. Flagellin from P. pabuli ALJ109b demonstrates high Te (0) forming activity in neutral to basic conditions in a range of temperatures from 20°C to 37°C. In conclusion, the first metabolic characterization of a strain of P. pabuli response to Te (IV) reveals a highly resistant strain with a unique Te (IV) proteomic response. This strain, and its flagellin, display, all the features of potential tools for Te nanoparticle production.

2017 ◽  
Vol 71 (2) ◽  
pp. 155-165 ◽  
Aleksandar Dosic ◽  
Dragana Tomasevic-Pilipovic ◽  
Miladin Gligoric ◽  
Bozo Dalmacija ◽  
Djurdja Kerkez ◽  

Increasing amounts of residues and waste materials coming from industrial activities in different processes have become an increasingly urgent problem for the future. The paper presents the problem of mine tailings generated in mine ?Sase? (Republic of Srpska, Bosnia and Herzegovina) with high metal content (Pb, Cu and Zn). Dumpsite of this tailing represents potential risk for water bodies in the vicinity of this location. Chosen treatment process was stabilization/solidification (S/S). Inorganic agents used in this study were fly ash and red mud that represent secondary industrial waste generated on locations relatively near the mine. Therefore, their application can be used as an example of a sustainable solution of regional environmental problem. Further investigations are related to the impact of various factors on metals leaching from mine tailings solidified/stabilized material using the above mentioned immobilization agents. The performance of the immobilizing procedures was examined using several leaching tests: ANS 16.1, TCLP, DIN, MWLP. The results indicated that all S/S samples can be considered as non-hazardous waste, as all leached metal concentrations met the set criteria. These results will further enable the modelling of metals behaviour during long-term leaching from treated mine tailing. The data are invaluable in terms of economically and environmentally sound management of mine tailing.

2020 ◽  
Vol 12 (4-2) ◽  
pp. 277-290
Natalia Bozo ◽  
Ekaterina Malysheva ◽  
Nadezhda Filatyeva ◽  

Machine building is a leading industry in advanced countries. It provides many industries with machines and equipment as it is the main link in the system of intersectoral linkages. Innovations in engineering create the basis for increasing production and qualitative changes in the structure of industry, that creates a synergistic effect for the economy as a whole. Thus, it secures full employment for highly skilled employees, promotes the growth of the level of educational background of the population and contributes to the development of the scientific and technological potential of the country. However, underinvestment in innovation technologies and processes, a limited character of the import substitution model and an insufficient support of the export model greatly hinder rapid and effective development of the machine-building industry. Specific peculiarities of the machine building development such as R&D intensity, labor intensity, and the intensity of metal use, the need in cooperation and consumer orientation influence their geographic location. Despite the decline in the number of enterprises and employment in the machine-building industry, the industry’s contribution to the GVA is stable. At the same time, the state’s interest in the development of all sectors with high added value will influence the future of engineering. In statistics innovative activity in mechanical engineering began to be considered relatively recently. The highest rates of innovation are high-tech engineering. The state’s share in supporting innovation in both manufacturing and engineering is increasing. The coefficients of specialization are calculated for all Federal districts and each of the 85 subjects of the Federation for the period from 2007 to 2017. Calculations were carried out for all types of economic activity, according to the classifier of OKVED. The specialization industries for each of the regions are identified. The group of regions where engineering is concentrated is defined. These 19 regions did not change their industry of specialization during the analyzed period from 2008 to 2017.

2020 ◽  
Vol 34 (3) ◽  
pp. 2861-2869 ◽  
Xu-Quan Tang ◽  
Jing-Cao Pu ◽  
Hong-Bing Zheng ◽  
Xu-De Yu ◽  
Xue-Feng Chen ◽  

2018 ◽  
Vol 792 ◽  
pp. 133-139 ◽  
Toapanta Germania ◽  
Caterine Donoso ◽  
María José Cárdenas ◽  
Amón Bolívar ◽  
Vladimir Ortiz

Spent catalysts contain metals that have a high added value. From all metals, lanthanum has attracted a lot of attention due to the growing demand in the high-tech. The spent catalyst of the hydrotreatment unit is a material composed of lanthanum-enriched matrix of amorphous aluminosilicates. The experiment was carried out with a spent catalyst with a constant particle size of 90 μm. The treatments were obtained applying of two level factorial design to investigate the effect of following factors: temperature (20 - 60 °C), nitric acid concentration (3 - 6 M), leaching time (1 - 4 h) and percent solids (10 - 20 %). The research is carried out in two steps process: pretreatment of the catalyst and leaching with nitric acid. The leaching results show a yield of lanthanum of 99.44% using the following conditions: temperature (20 °C), nitric acid concentration (3M), leaching time (1 h), percent solids (20%) and 300 rpm. The principal analysis of the spent catalyst was carried out using the X-Ray Fluorescence (XRF) technique, 3.08%, while the percentage of lanthanum recovery in the extract, washing and refining was carried out using the Inductive Coupling Plasma (ICP) technique.

1995 ◽  
Vol 10 (7) ◽  
pp. 1700-1709 ◽  
Steven W. Webb ◽  
W.E. Jackson

High-pressure, high temperature (HPHT) annealing of synthetic type I diamond crystals at 1200–1700 °C and 50–60 kbar was found to induce aggregate-nitrogen dissociation and metal coalescence as well as heal diamond lattice dislocations. For crystals with low levels of metal inclusions, HPHT annealing was observed to increase the average compressive fracture strength of the crystals by apparently strengthening the strongest crystals of the population. Crystals with high metal-content, or otherwise of low quality, are weakened by anncaling. Strengthening is believed to occur by locally stabilizing the diamond lattice by healing lattice dislocations as well as dispersing nitrogen within the lattice. A general model is presented that ties together these results with those of other researchers.

2013 ◽  
Vol 115 ◽  
pp. 115-121 ◽  
Miguel Molina-Sabio ◽  
Mateus Carvalho Monteiro de Castro ◽  
Manuel Martinez-Escandell ◽  
Francisco Rodríguez-Reinoso

2002 ◽  
Vol 16 (2) ◽  
pp. 97-104 ◽  
P.C. van der Sijde ◽  
G. Vogelaar ◽  
A. Hoogeveen ◽  
H. Ligtenberg ◽  
M. van Velzen

From 1998 to 2001 a consortium of regional partners – the University of Twente, the Enschede Foundation Business & Science Park, the Regional Development Agency, and the Municipality of Enschede – carried out a project to attract new business to the area of Twente and to the Business & Science Park in particular. In that period, a well-functioning structure for cooperation among the partners was established in order to attract companies in sectors related to the principal research areas of the university Cooperation in the consortium is analysed using the ‘4S’ model (scope of cooperation; skills; scale; social networks) to identify the added value of the partners in this endeavour. It emerges that the four parties were complementary to each other. In total, some 13 companies have been attracted to the region, 161 jobs have been created and it is expected that in the next five years those 13 companies will create around 250 new jobs. Interviews were carried out with the companies to assess the value for them of the university's technology research and facilities. The results indicate that the technological expertise of the university was and remains one of the key attractions of the region for the companies.

Liudmyla Mekshun ◽  
Maksym Zabashtanskyi

The article analyzes the current state and prospects of financial development of labor potential in Ukraine. The comparative analysis of financial support of labor stimulation in the leading countries of the world is carried out, the factors of stimulating and limiting influence on a condition of labor potential of the country are grouped. It is determined that the key problems hindering the development of labor potential in the country are the low level of development of the national economy, associated with a significant backlog of technological capabilities, moral and physical depreciation of fixed assets, the inability to create an innovative product with high added value. It is substantiated that in order to develop the national economy, the key condition is the creation of high-tech production that can ensure the competitiveness of its own products on the world market, which can be done only with adequate financial support to stimulate labor potential. It is proved that adequate financial support for labor incentives should be a key condition for the generation and preservation of labor potential necessary for the effective functioning of the national economy.

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