scholarly journals Exogenous DA-6 Improves the Low Night Temperature Tolerance of Tomato Through Regulating Cytokinin

2021 ◽  
Vol 11 ◽  
Author(s):  
Jiazhi Lu ◽  
Pengxiao Guan ◽  
Jiamao Gu ◽  
Xiaolong Yang ◽  
Feng Wang ◽  
...  
Keyword(s):  
Low Temperature ◽  
Plant Growth ◽  
Defense Mechanisms ◽  
Negative Impact ◽  
Temperature Response ◽  
Chlorophyll Synthesis ◽  
Temperature Tolerance ◽  
Photochemical Activity ◽  
Night Temperature ◽  
Cold Response

Low night temperature (LNT) causes environmental stress and has a severe and negative impact on plant growth and productivity. Synthetic elicitors can regulate plant growth and induce defense mechanisms from this type of stress. Here, we evaluated the effect of the exogenous growth regulator diethyl aminoethyl hexanoate (DA-6) in tomato leaf response to LNT stress. Our results showed that exogenous DA-6 activates the expression of chlorophyll synthesis and photosystem-related genes, and results in higher photosynthetic activity and chlorophyll production. Furthermore, DA-6 can regulate the synthesis of endogenous cytokinin (CTK) and the expression of decomposition genes to stabilize chloroplast structure, reduce oxidative damage, and maintain the photochemical activity of tomato leaves under LNT stress. DA-6 maintains a high level of ABA content and induces the expression of CBF genes, indicating that DA-6 may participate in the cold response signaling pathway and induce the expression of downstream low temperature response genes and accumulation of compatible osmolytes. This study unravels a mode of action by which plant growth regulators can improve low temperature tolerance and provides important considerations for their application to alleviate the harmful effects of cold stress.

scholarly journals Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tânia Pinheiro ◽  
Ka Ying Florence Lip ◽  
Estéfani García-Ríos ◽  
Amparo Querol ◽  
José Teixeira ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Temperature Response ◽  
Laboratory Strain ◽  
Temperature Tolerance ◽  
Anaerobic Conditions ◽  
Cold Response ◽  
Proteomic Data ◽  
Response To Temperature

AbstractElucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains in all conditions was performed by sequential window acquisition of all theoretical spectra-mass spectrometry (SWATH-MS), allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.

Temperature Regulation of the Development of Frost Hardiness in Pinus radiata D. Don

1983 ◽  
Vol 10 (6) ◽  
pp. 539 ◽  
Author(s):  
DH Greer
Keyword(s):  
Low Temperature ◽  
Temperature Response ◽  
Frost Hardiness ◽  
Natural Environments ◽  
Response Model ◽  
Night Temperature ◽  
Rate Of Development ◽  
Relative Contribution ◽  
Hardening Process ◽  
Hardening Treatment

The development of frost hardiness in seedlings of P. radiata was followed using a photoperiod of 9 h and a constant day temperature of 12°C. The seedlings were preconditioned to a night temperature of 6.5°C for 35 days and 4°C for 7 days. The temperature was then reduced to -4°C for 66 days in one hardening treatment (designated 12/-4°C) and to 3°C for 136 days in another (designated 12/3°C). At the end of those periods, the night temperature was increased back to 6.5°C for a further 15 days. Frost hardiness was determined at regular intervals by exposing the seedlings to a series of artificial frosts. During the low temperature regime, frost hardiness developed continuously in seedlings from both treatments, from -5 to -9.5°C in the 12/3°C treatment and to -16.5°C in the 12/-4°C treatment. The rate of development of frost hardiness was constant at constant temperatures. Using a temperature response model, specific rates of hardening were estimated for both this and an earlier experiment, and found to be approximately linear against temperature. The analysis establishes that temperature controls the hardening process of P. radiata by regulating the rate of development of frost hardiness. Frost hardiness was also shown to be related to thermal time. Before this relationship could be useful in predicting frost hardiness of field-grown seedlings, the relative contribution of the photoperiod-induced and temperature-induced hardening in natural environments needs to be more clearly defined.

scholarly journals Positive and negative effects of nanoparticles on plants and their applications in agriculture

2019 ◽  
Vol 6 (2) ◽  
pp. 232-242 ◽  
Author(s):  
Pooja Goswami ◽  
Sonali Yadav ◽  
Jyoti Mathur
Keyword(s):  
Plant Growth ◽  
Negative Impact ◽  
Plant Protection ◽  
Growth Promotion ◽  
Chlorophyll Synthesis ◽  
Production Plant ◽  
Negative Effects ◽  
Research Areas ◽  
Nano Medicine ◽  
Global Food

Nanotechnology is the promising field with its wide applications in biotechnology, pharmaceutical science, drug targeting, nano-medicine and other research areas. This review highlights the positive and negative impact of nanoparticles on plants and its wide applications in agricultural sciences. Effect of NPs in terms of seed germination, growth promotion and enhancement of metabolic rate has been evaluated by several scientific researches. However, NPs also exert their negative effects such as suppression of plant growth, inhibition of chlorophyll synthesis, photosynthetic efficiency etc. Effects of NPs can be either positive or negative it depending upon the plant species and type of nanoparticles used & its concentration. Modern nano-biotechnological tools have a great potential to increase food quality, global food production, plant protection, detection of plant and animal diseases, monitoring of plant growth nano-fertilizers, nano-pesticide, nano-herbicides and nano-fungicides.

scholarly journals Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions

2020 ◽  
Author(s):  
Tânia Pinheiro ◽  
Ka Ying Florence Lip ◽  
Estéfani García-Ríos ◽  
Amparo Querol ◽  
José Teixeira ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Temperature Response ◽  
Laboratory Strain ◽  
Temperature Tolerance ◽  
Anaerobic Conditions ◽  
Cold Response ◽  
Proteomic Data ◽  
Response To Temperature

AbstractElucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains was performed by SWATH-MS, allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.

scholarly journals Morpho-Physiological Characterization of Diverse Rice Genotypes for Seedling Stage High- and Low-Temperature Tolerance

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 112
Author(s):  
Kambham Raja Reddy ◽  
Akanksha Seghal ◽  
Salah Jumaa ◽  
Raju Bheemanahalli ◽  
Naqeebullah Kakar ◽  
...  
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Developmental Plasticity ◽  
Temperature Response ◽  
Temperature Tolerance ◽  
Seedling Stage ◽  
Response Index ◽  
High Temperature Tolerance ◽  
Early Seedling ◽  
Rice Genotypes

Extreme temperatures are considered one of the main constraints that limit the growth and development of rice. We elucidated the root and shoot developmental plasticity of 64 rice genotypes during early seedling establishment, using the sunlit plant growth chambers at 22/14 (low), 30/22 (optimum), and 38/30 °C (high) day/night temperatures. Low temperature severely inhibited 23 traits, such as shoot (68%), root (57%), and physiological (35%) attributes. On the contrary, the high temperature positively affected most of the shoot (48%) and root (31%) traits, except root diameter and root/shoot ratio, compared with the optimum. Alternatively, leaf chlorophyll fluorescence-associated parameters declined under low (34%) and high (8%) temperatures. A weak correlation between cumulative high-temperature response index (CHTRI) and cumulative low-temperature response index (CLTRI) indicates the operation of different low- and high-temperature tolerance mechanisms at the early seedling stage. Groups of distinct rice genotypes associated with low or high-temperature tolerance were selected based on CHTRI and CLTRI. The genotypes that commonly performed well under low and high temperatures (IR65600-81-5-2-3, CT18593-1-7-2-2-5, RU1504114, RU1504122, Bowman, and INIA Tacuari) will be valuable genetic resources for breeders in developing early-season high- and low-temperature-tolerant genotypes for a broad range of both tropical and temperate rice-growing environments.

scholarly journals Identification of key gene networks controlling vernalization development characteristics of Isatis indigotica by full-length transcriptomes and gene expression profiles

2021 ◽  
Author(s):  
Pan Wang ◽  
Dong Liu ◽  
Fu-Hong Yang ◽  
Hui Ge ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Gene Networks ◽  
Expression Profiles ◽  
Soluble Sugar ◽  
Differential Expression Analysis ◽  
Temperature Response ◽  
Temperature Tolerance ◽  
Full Length ◽  
Raw Material ◽  
Isatis Indigotica

AbstractIsatis indigotica Fort., as a common Chinese medicinal raw material, will lose its medicinal value if it blooms early, so it is highly valuable to clarify the induction mechanism of the vernalization of I. indigotica at low temperature. In this study, the concentrations of soluble sugar, proline, glutathione and zeatin in two germplasms of I. indigotica with different degrees of low temperature tolerance (Y1 and Y2) were determined at 10 days, 20 days and 30 days of low-temperature treatment, and the full-length transcriptome of 24 samples was sequenced by Nanopore sequencing with Oxford Nanopore Technologies (ONT). After that, the data of transcripts involved in the vernalization of I. indigotica at low temperature were obtained, and these transcripts were identified using weighted gene co-expression network analysis (WGCNA). The results revealed the massive accumulation of soluble sugar and proline in Y1 and Y2 after low temperature induction. A total of 18,385 new transcripts, 6168 transcription factors and 470 lncRNAs were obtained. Differential expression analysis showed that gibberellin, flavonoids, fatty acids and some processes related to low temperature response were significantly enriched. Eight key transcripts were identified by WGCNA, among which ONT.14640.1, ONT.9119.1, ONT.13080.2 and ONT.16007.1 encodes a flavonoid transporter, 9-cis-epoxycarotenoid dioxygenase 3 (NCED3), growth factor gene and L-aspartate oxidase in plants, respectively. It indicated that secondary metabolites such as hormones and flavonoids play an important role in the vernalization of I. indigotica. qRT-PCR proved the reliability of transcriptome results. These results provide important insights on the low-temperature vernalization of I. indigotica, and provide a research basis for analyzing the vernalization mechanism of I. indigotica.

scholarly journals Application of Trehalose and Salicylic Acid Mitigates Drought Stress in Sweet Basil and Improves Plant Growth

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1078
Author(s):  
Faisal Zulfiqar ◽  
Jianjun Chen ◽  
Patrick M. Finnegan ◽  
Adnan Younis ◽  
Muhammad Nafees ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salicylic Acid ◽  
Drought Stress ◽  
Plant Growth ◽  
Defense Mechanisms ◽  
Negative Impact ◽  
Field Capacity ◽  
Antioxidant Responses ◽  
Sweet Basil ◽  
Combined Application

Trehalose (Tre) and salicylic acid (SA) are increasingly used to mitigate drought stress in crop plants. In this study, a pot experiment was performed to study the influence of Tre and SA applied individually or in combination on the growth, photosynthesis, and antioxidant responses of sweet basil (Ocimum basilicum L.) exposed to drought stress. Basil plants were watered to 60% or 100% field capacity with or without treatment with 30 mM Tre and/or 1 mM SA. Drought negatively affected growth, physiological parameters, and antioxidant responses. Application of Tre and/or SA resulted in growth recovery, increased photosynthesis, and reduced oxidative stress. Application of Tre mitigated the detrimental effects of drought more than SA. Furthermore, co-application of Tre and SA largely eliminated the negative impact of drought by reducing oxidative stress through increased activities of antioxidant enzymes superoxide dismutase, peroxidase, and catalase, as well as the accumulation of the protective osmolytes proline and glycine betaine. Combined Tre and SA application improved water use efficiency and reduced the amount of malondialdehyde in drought-stressed plants. Our results suggested that combined application of Tre and SA may trigger defense mechanisms of sweet basil to better mitigate oxidative stress induced by drought stress, thereby improving plant growth.

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