scholarly journals Identifying Molecular Markers of Successful Graft Union Formation and Compatibility

2020 ◽  
Vol 11 ◽  
Author(s):  
Grégoire Loupit ◽  
Sarah Jane Cookson
Keyword(s):  
Current Knowledge ◽  
Genetic Research ◽  
Transcript Abundance ◽  
Redox Status ◽  
Union Formation ◽  
Graft Union ◽  
Expression Of Genes ◽  
Stress Formation ◽  
Graft Compatibility ◽  
Graft Incompatibility

Grafting is a technique used for millennia for vegetative propagation, especially in perennial fruit crops. This method, used on woody and herbaceous plants, can improve several agronomic characteristics, such as yield or vigor, as well as tolerance to biotic and abiotic stresses. However, some scion/rootstock combinations suffer from poor graft compatibility, i.e., they are unable to form and/or sustain a successful graft union. Identifying symptoms of graft incompatibility is difficult because they are not always present in the first years after grafting and in most cases the causes of incompatibility are still poorly understood. Studies of changes in transcript abundance during graft union formation indicate that grafting responses are similar to responses to wounding and include the differential expression of genes related to hormone signaling, oxidative stress, formation of new vascular vessels, cell development, and secondary metabolites, in particular polyphenols. This review summarizes current knowledge of the changes in transcript abundance, redox status and metabolites accumulation during graft union formation and in cases of graft incompatibility. The goal of this review is to discuss the possibility of identifying marker transcripts, enzyme activities and/or metabolites of grafting success and graft compatibility which could be used to score grafting success for genetic research and in breeding programs. We highlight gaps in current knowledge and potential research directions in this field.

scholarly journals Sugars promote graft union development in the heterograft of cucumber onto pumpkin

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Li Miao ◽  
Qing Li ◽  
Tian-shu Sun ◽  
Sen Chai ◽  
Changlin Wang ◽  
...  
Keyword(s):  
Sugar Content ◽  
Healing Process ◽  
Energy Charge ◽  
Sugar Metabolism ◽  
Union Formation ◽  
Graft Union ◽  
Positive Role ◽  
Exogenous Glucose ◽  
Expression Of Genes ◽  
Graft Interface

AbstractThe use of heterografts is widely applied for the production of several important commercial crops, but the molecular mechanism of graft union formation remains poorly understood. Here, cucumber grafted onto pumpkin was used to study graft union development, and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated. Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection, and the scion showed evident callus production compared with the rootstock 3 days after grafting. Consistently, transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development, the cell cycle, and sugar metabolism during both vascular reconnection and healing, indicating distinct mechanisms. Additionally, lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection. Next, we found that the healing process of grafted etiolated seedlings was significantly delayed, and graft success, xylem reconnection, and the growth of grafted plants were enhanced by exogenous glucose. This demonstrates that graft union formation requires the correct sugar content. Furthermore, we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin (TOR) inhibitor AZD-8055, and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment. Taken together, our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future.

scholarly journals Molecular and physiological characterization of the effects of auxin-enriched rootstock on grafting

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Longmei Zhai ◽  
Xiaomin Wang ◽  
Dan Tang ◽  
Qi Qi ◽  
Huseyin Yer ◽  
...  
Keyword(s):  
Callus Formation ◽  
Ethylene Biosynthesis ◽  
Union Formation ◽  
Biosynthetic Gene ◽  
Graft Union ◽  
Specific Expression ◽  
Physiological Characterization ◽  
Graft Healing ◽  
Expression Of Genes ◽  
Or Gene

AbstractsGrafting is a highly useful technique, and its success largely depends on graft union formation. In this study, we found that root-specific expression of the auxin biosynthetic gene iaaM in tobacco, when used as rootstock, resulted in more rapid callus formation and faster graft healing. However, overexpression of the auxin-inactivating iaaL gene in rootstocks delayed graft healing. We observed increased endogenous auxin levels and auxin-responsive DR5::GUS expression in scions of WT/iaaM grafts compared with those found in WT/WT grafts, which suggested that auxin is transported upward from rootstock to scion tissues. A transcriptome analysis showed that auxin enhanced graft union formation through increases in the expression of genes involved in graft healing in both rootstock and scion tissues. We also observed that the ethylene biosynthetic gene ACS1 and the ethylene-responsive gene ERF5 were upregulated in both scions and rootstocks of the WT/iaaM grafts. Furthermore, exogenous applications of the ethylene precursor ACC to the junction of WT/WT grafts promoted graft union formation, whereas application of the ethylene biosynthesis inhibitor AVG delayed graft healing in WT/WT grafts, and the observed delay was less pronounced in the WT/iaaM grafts. These results demonstrated that elevated auxin levels in the iaaM rootstock in combination with the increased auxin levels in scions caused by upward transport/diffusion enhanced graft union formation and that ethylene was partially responsible for the effects of auxin on grafting. Our findings showed that grafting success can be enhanced by increasing the auxin levels in rootstocks using transgenic or gene-editing techniques.

Effects of Rootstock Source on the Size, Cone Production, and Compatibility of Douglas-Fir Grafts

1992 ◽  
Vol 7 (3) ◽  
pp. 73-77
Author(s):  
Ingemar Karlsson ◽  
Jack H. Woods
Keyword(s):  
Pseudotsuga Menziesii ◽  
Douglas Fir ◽  
Fast Growth ◽  
Production Potential ◽  
Graft Union ◽  
Cone Production ◽  
Graft Compatibility ◽  
Graft Incompatibility

Abstract Scions of coastal Douglas-fir (Pseudotsuga menziesii) were grafted on rootstock from coastal, northern interior and southern interior origin. Data on ramet size, cone production, and graft compatibility were collected. Twelve years after grafting significant differences for most crown and stem traits were found among the rootstock sources, with coastal rootstock resulting in the fastest scion growth. Northern interior rootstock sources produced larger grafts than southern interior rootstock. Significant graft union appearance and cone production differences were not detected, but coastal sources consistently had higher graft compatibility and healthier unions. It is concluded that, for coastal Douglas-fir, the use of a vigorous coastal rootstock source that promotes fast growth in the scion, and has high graft compatibility potential, is the most desirable for coastal locations. This will result in fewer losses due to graft incompatibility, faster crown development, more options for crown management and increased cone production potential. West. J. Appl. For. 7(3):73-77.

scholarly journals A DNS-metiláció szerepe és megváltozása az öregedés és a daganatos betegségek kialakulása során

2018 ◽  
Vol 159 (1) ◽  
pp. 3-15 ◽  
Author(s):  
Krisztina Andrea Szigeti ◽  
Orsolya Galamb ◽  
Alexandra Kalmár ◽  
Barbara Kinga Barták ◽  
Zsófia Brigitta Nagy ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Current Knowledge ◽  
Genetic Research ◽  
Regulation Of Transcription ◽  
Gene Promoters ◽  
Defence Mechanisms ◽  
Dna Mutation ◽  
Expression Of Genes ◽  
Initiation Of Replication ◽  
Disease Diagnostics

Abstract: Besides the genetic research, increasing number of scientific studies focus on epigenetic phenomena – such as DNA methylation – regulating the expression of genes behind the phenotype, thus can be related to the pathomechanism of several diseases. In this review, we aim to summarize the current knowledge about the evolutionary appearance and functional diversity of DNA methylation as one of the epigenetic mechanisms and to demonstrate its role in aging and cancerous diseases. DNA methylation is also characteristic/also appear to prokaryotes, eukaryotes and viruses. In prokaryotes and viruses, it provides defence mechanisms against extragenous DNA. DNA methylation in prokaryotes plays a significant role in the regulation of transcription, the initiation of replication and in Dam-directed mismatch repair. In viruses, it participates not only in defence mechanisms, but in the assembly of capsids as well which is necessary for spreading. In eukaryotes, DNA methylation is involved in recombination, replication, X chromosome inactivation, transposon control, regulation of chromatin structure and transcription, and it also contributes to the imprinting phenomenon. Besides the above-mentioned aspects, DNA methylation also has an evolutionary role as it can change DNA mutation rate. Global hypomethylation appearing during aging and in cancerous diseases can lead to genetic instablility and spontaneous mutations through its role in the regulation of transposable elements. Local hypermethylated alterations such as hypermethylation of SFRP1, SFRP2, DKK1 and APC gene promoters can cause protein expression changes, thus contribute to development of cancer phenotype. DNA methylation alterations during aging in cancerous diseases support the importance of epigenetic research focusing on disease diagnostics and prognostics. Orv Hetil. 2018; 159(1): 3–15.

scholarly journals Light quality and quantity affect graft union formation of tomato plants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ahmed Fathy Yousef ◽  
Muhammad Moaaz Ali ◽  
Hafiz Muhammad Rizwan ◽  
Ahmed Gomaa Gad ◽  
Dangdi Liang ◽  
...  
Keyword(s):  
Vascular Tissue ◽  
Red Light ◽  
Anatomical Study ◽  
Union Formation ◽  
Graft Union ◽  
Tomato Plants ◽  
Light Emitting ◽  
Tomato Seedlings ◽  
Expression Of Genes

AbstractIt is already known that there are many factors responsible for the successful formation of a graft union. However, the role of light has been little studied. In an anatomical study, Scanning Electronic Microscope (SEM) was used to explore the effects of different light-emitting diodes (LEDs) on graft union formation in grafted tomato. In addition, the expression genes related to Auxin hormone signaling pathway (SAUR67, AUX1, ARF30, and LAX3) was investigated. The obtained results showed that the concrescence process occurred faster under R7:B3 light conditions, as compared to blue (B) and white fluorescent (WFL) lights. Red light application caused a delay in the vascular tissue differentiation, which may lead to callus development on both sides, causing junctional failure and resulting in ineffective graft junctional arrangement. The expression of genes related to Auxin hormone significantly increased by R7:B3 application. We suggest that LED spectra affects the graft development of tomato plants and can improve the performance of grafted tomato seedlings.

scholarly journals Graft Compatibility among Chestnut (Castanea) Species

1994 ◽  
Vol 119 (6) ◽  
pp. 1127-1132 ◽  
Author(s):  
Hongwen Huang ◽  
J.D. Norton ◽  
G.E. Boyhan ◽  
B.R. Abrahams
Keyword(s):  
Graft Failure ◽  
Graft Union ◽  
Genetic Incompatibility ◽  
Chinese Chestnut ◽  
Growing Seasons ◽  
Peroxidase Isozymes ◽  
Lower Success Rate ◽  
Graft Compatibility ◽  
Graft Incompatibility ◽  
Graft Success

Graft compatibility was investigated for 15 Chinese chestnut (Castanea mollissima Bl.) cultivars, nine American chestnut [C. dentata (Marsh.) Borkh.] selections, six Japanese chestnut (C. crenata Sieb.) cultivars, and two putative Japanese hybrids on two known rootstocks of Chinese chestnut. Intraspecific grafting of Chinese chestnut resulted in 80% success after two growing seasons. An unusual anatomical structure of the chestnut stem had a significant effect on graft success. The phloem fiber bundles related to graft failure are described in the study. Interspecific grafts of seven American and five Japanese chestnut selections resulted in ≥70% success. The putative Japanese hybrids had a significantly lower success rate (<50%) regardless of rootstocks. A marked graft incompatibility was found in one Japanese/Chinese and two American/Chinese combinations. Graft incompatibility related to morphological abnormalities at the graft union was also observed in interspecific grafts. Comparisons of cambial isoperoxidase isozymes between successful and unsuccessful grafts did not support the hypothesis that peroxidase isozymes are indicators of rootstock-scion compatibility. The results suggest that genetic incompatibility is not a major cause of graft failure in Chinese chestnut.

Plant Compounds for the Treatment of Diabetes, a Metabolic Disorder: NF-κB as a Therapeutic Target

2020 ◽  
Vol 26 (39) ◽  
pp. 4955-4969
Author(s):  
Ravi Sahukari ◽  
Jyothi Punabaka ◽  
Shanmugam Bhasha ◽  
Venkata S. Ganjikunta ◽  
Shanmugam K. Ramudu ◽  
...  
Keyword(s):  
Diabetic Complications ◽  
Current Knowledge ◽  
World Population ◽  
Future Studies ◽  
Expression Of Genes ◽  
Treatment Of Diabetes ◽  
Online Library ◽  
And Oxidative Stress ◽  
Stress Activation ◽  
Plant Compounds

Background: The prevalence of diabetes in the world population hás reached 8.8 % and is expected to rise to 10.4% by 2040. Hence, there is an urgent need for the discovery of drugs against therapeutic targets to sojourn its prevalence. Previous studies proved that NF-κB serves as a central agent in the development of diabetic complications. Objectives: This review intended to list the natural plant compounds that would act as inhibitors of NF-κB signalling in different organs under the diabetic condition with their possible mechanism of action. Methods: Information on NF-κB, diabetes, natural products, and relation in between them, was gathered from scientific literature databases such as Pubmed, Medline, Google scholar, Science Direct, Springer, Wiley online library. Results and Conclusion: NF-κB plays a crucial role in the development of diabetic complications because of its link in the expression of genes that are responsible for organs damage such as kidney, brain, eye, liver, heart, muscle, endothelium, adipose tissue and pancreas by inflammation, apoptosis and oxidative stress. Activation of PPAR-α, SIRT3/1, and FXR through many cascades by plant compounds such as terpenoids, iridoids, flavonoids, alkaloids, phenols, tannins, carbohydrates, and phytocannabinoids recovers diabetic complications. These compounds also exhibit the prevention of NF-κB translocation into the nucleus by inhibiting NF-κB activators, such as VEGFR, RAGE and TLR4 receptors, which in turn, prevent the activation of many genes involved in tissue damage. Current knowledge on the treatment of diabetes by targeting NF-κB is limited, so future studies would enlighten accordingly.

scholarly journals Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Duyên Prodhomme ◽  
Josep Valls Fonayet ◽  
Cyril Hévin ◽  
Céline Franc ◽  
Ghislaine Hilbert ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolism ◽  
Fruits And Vegetables ◽  
Cell Formation ◽  
Union Formation ◽  
Primary Metabolism ◽  
Graft Union ◽  
Primary And Secondary Metabolites ◽  
Primary And Secondary Metabolism ◽  
Graft Interface

Abstract Background Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering stems (canes) of grapevine and the graft interface tissues (containing some woody stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. Results A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding woody stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding woody stem tissues. Conclusions This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes.

scholarly journals “Too much guts and not enough brains”: (epi)genetic mechanisms and future therapies of Hirschsprung disease — a review

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Emilie G. Jaroy ◽  
Lourdes Acosta-Jimenez ◽  
Ryo Hotta ◽  
Allan M. Goldstein ◽  
Ragnhild Emblem ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nervous System ◽  
Enteric Nervous System ◽  
Hirschsprung's Disease ◽  
Hirschsprung’S Disease ◽  
Current Knowledge ◽  
Hirschsprung Disease ◽  
Gut Development ◽  
Dna And Rna ◽  
Expression Of Genes

Abstract Hirschsprung disease is a neurocristopathy, characterized by aganglionosis in the distal bowel. It is caused by failure of the enteric nervous system progenitors to migrate, proliferate, and differentiate in the gut. Development of an enteric nervous system is a tightly regulated process. Both the neural crest cells and the surrounding environment are regulated by different genes, signaling pathways, and morphogens. For this process to be successful, the timing of gene expression is crucial. Hence, alterations in expression of genes specific for the enteric nervous system may contribute to the pathogenesis of Hirschsprung’s disease. Several epigenetic mechanisms contribute to regulate gene expression, such as modifications of DNA and RNA, histone modifications, and microRNAs. Here, we review the current knowledge of epigenetic and epitranscriptomic regulation in the development of the enteric nervous system and its potential significance for the pathogenesis of Hirschsprung’s disease. We also discuss possible future therapies and how targeting epigenetic and epitranscriptomic mechanisms may open new avenues for novel treatment.

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