scholarly journals Proteomic Analysis of Fusarium oxysporum-Induced Mechanism in Grafted Watermelon Seedlings

2021 ◽  
Vol 12 ◽  
Author(s):  
Man Zhang ◽  
Jinhua Xu ◽  
Runsheng Ren ◽  
Guang Liu ◽  
Xiefeng Yao ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Fusarium Oxysporum ◽  
Proteomic Analysis ◽  
Disease Incidence ◽  
Two Dimensional Gel Electrophoresis ◽  
Proteomic Approach ◽  
Scavenging Capacity ◽  
Pathogenesis Related ◽  
Functional Implications ◽  
Related Proteins

Grafting can improve the resistance of watermelon to soil-borne diseases. However, the molecular mechanism of defense response is not completely understood. Herein, we used a proteomic approach to investigate the molecular basis involved in grafted watermelon leaf defense against Fusarium oxysporum f.sp. niveum (FON) infection. The bottle gourd rootstock-grafted (RG) watermelon seedlings were highly resistant to FON compared with self-grafted (SG) watermelon plants, with a disease incidence of 3.4 and 89%, respectively. Meanwhile, grafting significantly induced the activity of pathogenesis-related proteases under FON challenge. Proteins extracted from leaves of RG and SG under FON inoculation were analyzed using two-dimensional gel electrophoresis. Thirty-nine differentially accumulated proteins (DAPs) were identified and classified into 10 functional groups. Accordingly, protein biosynthetic and stress- and defense-related proteins play crucial roles in the enhancement of disease resistance of RG watermelon seedlings, compared with that of SG watermelon seedlings. Proteins involved in signal transduction positively regulated the defense process. Carbohydrate and energy metabolism and photosystem contributed to energy production in RG watermelon seedlings under FON infection. The disease resistance of RG watermelon seedlings may also be related to the improved scavenging capacity of reactive oxygen species (ROS). The expression profile of 10 randomly selected proteins was measured using quantitative real-time PCR, among which, 7 was consistent with the results of the proteomic analysis. The functional implications of these proteins in regulating grafted watermelon response against F. oxysporum are discussed.

scholarly journals Molecular Insights into the Role of Cysteine-Rich Peptides in Induced Resistance to Fusarium oxysporum Infection in Tomato Based on Transcriptome Profiling

2021 ◽  
Vol 22 (11) ◽  
pp. 5741
Author(s):  
Marina P. Slezina ◽  
Ekaterina A. Istomina ◽  
Tatyana V. Korostyleva ◽  
Alexey S. Kovtun ◽  
Artem S. Kasianov ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Search Algorithm ◽  
Transcriptome Profiling ◽  
Stress Reactions ◽  
Lipid Transfer ◽  
Solanum Lycopersicum L ◽  
Signaling Peptides ◽  
Pathogenesis Related ◽  
Related Proteins

Cysteine-rich peptides (CRPs) play an important role in plant physiology. However, their role in resistance induced by biogenic elicitors remains poorly understood. Using whole-genome transcriptome sequencing and our CRP search algorithm, we analyzed the repertoire of CRPs in tomato Solanum lycopersicum L. in response to Fusarium oxysporum infection and elicitors from F. sambucinum. We revealed 106 putative CRP transcripts belonging to different families of antimicrobial peptides (AMPs), signaling peptides (RALFs), and peptides with non-defense functions (Major pollen allergen of Olea europaea (Ole e 1 and 6), Maternally Expressed Gene (MEG), Epidermal Patterning Factor (EPF)), as well as pathogenesis-related proteins of families 1 and 4 (PR-1 and 4). We discovered a novel type of 10-Cys-containing hevein-like AMPs named SlHev1, which was up-regulated both by infection and elicitors. Transcript profiling showed that F. oxysporum infection and F. sambucinum elicitors changed the expression levels of different overlapping sets of CRP genes, suggesting the diversification of functions in CRP families. We showed that non-specific lipid transfer proteins (nsLTPs) and snakins mostly contribute to the response of tomato plants to the infection and the elicitors. The involvement of CRPs with non-defense function in stress reactions was also demonstrated. The results obtained shed light on the mode of action of F. sambucinum elicitors and the role of CRP families in the immune response in tomato.

scholarly journals Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

2020 ◽  
Author(s):  
Lei Zhang ◽  
Lina Liu ◽  
Shu Li ◽  
Tingting Bai ◽  
Shengtao Xu ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Rna Binding ◽  
Xylem Sap ◽  
Induced Response ◽  
Soilborne Disease ◽  
Related Proteins ◽  
Race 4

Abstract Background Fusarium wilt is a destructive soilborne disease of banana caused by Fusarium oxysporum f. sp. cubense ( Foc ), especially Tropical Race 4 (TR4), which is a xylem-invading fungus. It was evident that xylem sap contained macromolecules, such as proteins, involved in disease-resistance processes. However, there is no research to analyze changes in banana xylem sap proteins response to TR4 to date. Methods To gain an integrated understanding of differential protein expression in banana xylem sap during TR4 infection, we performed a comparative proteomic analysis of xylem sap in resistant ‘Pahang’ and susceptible ‘Brazilian’ bananas inoculated with TR4. Results A total of 1036 proteins were detected in xylem sap of both bananas, among which some proteins are involved in ‘signal transduction’, ‘environmental adaptation’, ‘biosynthesis of secondary metabolites’ and ‘lipid metabolism’, indicating that xylem sap contained defense-related proteins. A number of 129 differential expression proteins (DEPs) were identified in 4 possible pairs between resistant and susceptible tested combinations. Of these DEPs, hypersensitive-induced response protein 1 (HIR1), E3 ubiquitin ligase (E3) might play negative roles in ‘Pahang’ response to TR4 attack, whereas chalcone isomerase (CHI), glycine-rich RNA-binding protein (GRP), carboxylesterase (CXE) and GDSL lipase (GLIP) might play positive roles in ‘Pahang’ defense against TR4 infection. Conclusions Banana xylem sap contained defense-related proteins, among which HIRP1, E3, CHI, GRP, CXE and GLIP involved in banana defense against TR4. To our knowledge, this is first report to analyze changes in banana xylem sap proteins response to TR4, which help us to explore molecular mechanisms of banana resistant to Fusarium wilt.

scholarly journals 2-DE-based proteomic analysis of protein changes associated with etiolated mesocotyl growth in Zea mays

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Liangjie Niu ◽  
Zhaokun Wu ◽  
Hui Liu ◽  
Xiaolin Wu ◽  
Wei Wang
Keyword(s):  
Zea Mays ◽  
Proteomic Analysis ◽  
Stress Proteins ◽  
Storage Proteins ◽  
Biological Processes ◽  
Protein Patterns ◽  
Two Dimensional Gel Electrophoresis ◽  
Oxidation Reduction ◽  
Shock Proteins ◽  
Related Proteins

Abstract Background The mesocotyl connects the coleoptilar node and the basal part of the seminal root of maize (Zea mays) seedling. The mesocotyl pushes the shoot of the seedling out of the soil during seed germination; thus, its growth is highly related to deep-sowing tolerance. Although many studies on the maize mesocotyl have been carried out at physiological and molecular levels, the proteomic changes associated with cellular and physiological activities during mesocotyl growth are still unknown. Results In the present study, the maize hybrid Zhengdan 958 was used to study mesocotyl growth and accompanying protein changes. The dark-grown etiolated mesocotyls exhibited a slow-fast-slow feature, with significant changes in the levels of indole-3-acetic acid (IAA) and cellulose and the activity of peroxidase (POD). In particular, POD activity increased with mesocotyl growth, showing higher activity at the mature (lower) end of the mesocotyl. For the proteomic analysis, soluble proteins were extracted from etiolated mesocotyls dark-grown for 48 h, 84 h, and 132 h, corresponding to the initial, rapid, and slow growth periods, respectively, and subjected to separation by two-dimensional gel electrophoresis (2-DE). As a result, 88 differentially abundant proteins (DAPs) were identified using MALDI-TOF-TOF analysis. At 48 h, most DAPs were stress proteins, heat shock proteins and storage proteins; at 84 h, oxidation/reduction proteins, carbohydrate biogenesis-related proteins and cytoskeleton-related proteins were highly accumulated; at 132 h, the most striking DAPs were those involved in the synthesis and modification of the cell wall and the biogenesis of carbohydrates. Gene ontology (GO) analysis showed that changes in the abundance and proportion of DAPs were consistent with cellular and physiological activities and biological processes during mesocotyl growth. The accumulation of nine DAPs of interest was verified by immunoblotting and RT-qPCR. Conclusions The present study revealed that the protein patterns in 2-D gels differed greatly with mesocotyl growth. At different growth periods, a specific set of DAPs participate in various biological processes and underlie the cellular and physiological activities of the mesocotyl. These results contributed to the understanding of mesocotyl growth and the cultivation of maize lines with deep-sowing tolerance.

scholarly journals Application of the ProteomeLab™ PF2D protein fractionation system in proteomic analysis for human genetic diseases

2012 ◽  
Vol 10 (3) ◽  
pp. 836-843
Author(s):  
Li Wang ◽  
Nanbert Zhong
Keyword(s):  
Proteomic Analysis ◽  
Biological Fluids ◽  
Genetic Disorders ◽  
Genetic Diseases ◽  
Two Dimensional ◽  
Protein Fractionation ◽  
Two Dimensional Gel Electrophoresis ◽  
Protein Mapping ◽  
Extraction And Separation ◽  
Proteomic Approach

AbstractProteomic analysis has been widely used in elucidating the mechanism of diseases. As a classical proteomic approach, two-dimensional gel electrophoresis (2DGE) has been commonly applied in finding differentially expressed proteins through a first dimension of separation by the isoelectric point (pI) of proteins and a second dimension of separation according to the molecular weight (MW) of proteins. Compared to 2DGE, a recently developed commercial system from Beckman Coulter, the two-dimensional protein fractionation (PF2D), separates proteins according to the pI of proteins in the first dimension followed by a second dimension of separation according to the degree of protein hydrophobicity. As a liquid-based fractionation system, PF2D could facilitate the extraction and separation of broader protein categories and improve reproducibility and quantification as well as be less labor-intensive, which are usually identified as limitations of a gel-based 2DGE platform. This review evaluates the applications of the PF2D system and discusses the perspectives and advantages of PF2D in the investigation of cancer and genetic disorders and in protein mapping in human biological fluids and cell cultures.

Induction of pathogenesis-related proteins in intercellular fluid by cadmium stress in barley (Hordeum vulgareL.) — A proteomic analysis

2011 ◽  
Vol 40 (Supplement 1) ◽  
pp. 164-175 ◽  
Author(s):  
V. Pós ◽  
É. Hunyadi-Gulyás ◽  
R. Caiazzo ◽  
I. Jócsák ◽  
K. Medzihradszky ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Cadmium Stress ◽  
Pathogenesis Related Proteins ◽  
Intercellular Fluid ◽  
Pathogenesis Related ◽  
Related Proteins

scholarly journals Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Yu-Dong Xu ◽  
Jian-Mei Cui ◽  
Yu Wang ◽  
Lei-Miao Yin ◽  
Chang-Ke Gao ◽  
...  
Keyword(s):  
Lavage Fluid ◽  
Specific Protein ◽  
Effector Proteins ◽  
Acupuncture Points ◽  
Two Dimensional Gel Electrophoresis ◽  
Drug Candidates ◽  
Beneficial Effects ◽  
Proteomic Approach ◽  
Altered Proteins ◽  
Related Proteins

Although the beneficial effects of acupuncture in asthma treatment have been well documented, little is known regarding the biological basis of this treatment. Changes in the lung proteome of acupuncture-treated rats with asthma onset were comparatively analyzed using a two-dimensional gel electrophoresis (2DE) and mass-spectrometry- (MS-) based proteomic approach. Acupuncture on specific acupuncture points appeared to improve respiratory function and reduce the total number of leukocytes and eosinophils in bronchoalveolar lavage fluid in OVA-induced asthma onset. Image analysis of 2DE gels revealed 32 differentially expressed acupuncture-specific protein spots in asthma onset; 30 of which were successfully identified as 28 unique proteins using LC-MS/MS. Bioinformatic analyses indicated that these altered proteins are most likely involved in inflammation-related biological functions, and the functional associations of these proteins result in an inflammation signaling pathway. Acupuncture regulates the pathway at different levels by regulating several key nodal proteins, including downregulating of proinflammatory proteins (e.g., S100A8, RAGE, and S100A11) and upregulating of anti-inflammatory proteins (e.g., CC10, ANXA5, and sRAGE). These deregulated inflammation-related proteins may mediate, at least in part, the antiasthmatic effect of acupuncture. Further functional investigation of these acupuncture-specific effector proteins could identify new drug candidates for the prophylaxis and treatment of asthma.

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