scholarly journals A Wild Allele of Pyrroline-5-Carboxylate Synthase1 Leads to Proline Accumulation in Spikes and Leaves of Barley Contributing to Improved Performance Under Reduced Water Availability

2021 ◽  
Vol 12 ◽  
Author(s):  
Felix Frimpong ◽  
Carel W. Windt ◽  
Dagmar van Dusschoten ◽  
Ali A. Naz ◽  
Michael Frei ◽  
...  
Keyword(s):  
Spring Barley ◽  
Water Shortage ◽  
Proline Accumulation ◽  
Introgression Lines ◽  
Reproductive Stage ◽  
Wild Allele ◽  
Intrinsic Water Use Efficiency ◽  
Improved Performance ◽  
Magnetic Resonance Imaging Mri ◽  
Barley Genotypes

Water stress (WS) during spike development strongly affects final grain yield and grain quality in cereals. Proline, an osmoprotectant amino-acid, may contribute to alleviating the effects of cell and tissue dehydration. We studied five spring barley genotypes contrasting in their drought response, including two introgression lines, S42IL-143 and S42IL-141, harboring a Pyrroline-5-carboxylate synthase1- P5cs1 allele originating from the wild barley accession ISR42-8. We tested the hypothesis that barley genotypes harboring a wild allele at P5cs1 locus are comparatively more drought-tolerant at the reproductive stage by inducing proline accumulation in their immature spikes. At the booting stage, we subjected plants to well-watered and WS treatments until physiological maturity. Several morpho-physiological traits had significant genotype by treatment interaction and reduction under WS. Varying levels of genotypic proline accumulation and differences in WS tolerance were observed. Spike proline accumulation was higher than leaf proline accumulation for all genotypes under WS. Also, introgression lines carrying a wild allele at P5cs1 locus had a markedly higher spike and leaf proline content compared with the other genotypes. These introgression lines showed milder drought symptoms compared with elite genotypes, remained photosynthetically active under WS, and maintained their intrinsic water use efficiency. These combined responses contributed to the achievement of higher final seed productivity. Magnetic resonance imaging (MRI) of whole spikes at the soft dough stage showed an increase in seed abortion among the elite genotypes compared with the introgression lines 15 days after WS treatment. Our results suggest that proline accumulation at the reproductive stage contributes to the maintenance of grain formation under water shortage.

scholarly journals Ppd-H1 integrates drought stress signals to control spike development and flowering time in barley

2020 ◽  
Author(s):  
Leonard Gol ◽  
Einar B Haraldsson ◽  
Maria von Korff
Keyword(s):  
Drought Stress ◽  
Developmental Plasticity ◽  
Floral Development ◽  
Spring Barley ◽  
Introgression Lines ◽  
Photoperiod Response ◽  
Severe Drought ◽  
Wild Type ◽  
Spike Development ◽  
Stress Signals

Abstract Drought impairs growth and spike development, and is therefore a major cause of yield losses in the temperate cereals barley and wheat. Here, we show that the photoperiod response gene PHOTOPERIOD-H1 (Ppd-H1) interacts with drought stress signals to modulate spike development. We tested the effects of a continuous mild and a transient severe drought stress on developmental timing and spike development in spring barley cultivars with a natural mutation in ppd-H1 and derived introgression lines carrying the wild-type Ppd-H1 allele from wild barley. Mild drought reduced the spikelet number and delayed floral development in spring cultivars but not in the introgression lines with a wild-type Ppd-H1 allele. Similarly, drought-triggered reductions in plant height, and tiller and spike number were more pronounced in the parental lines compared with the introgression lines. Transient severe stress halted growth and floral development; upon rewatering, introgression lines, but not the spring cultivars, accelerated development so that control and stressed plants flowered almost simultaneously. These genetic differences in development were correlated with a differential down-regulation of the flowering promotors FLOWERING LOCUS T1 and the BARLEY MADS-box genes BM3 and BM8. Our findings therefore demonstrate that Ppd-H1 affects developmental plasticity in response to drought in barley.

Apex and ear development in relation to the number of grains on the main-stem ears in spring barley (Hordeum distichon)

1991 ◽  
Vol 117 (1) ◽  
pp. 39-45 ◽  
Author(s):  
M. B.Garcia del Moral ◽  
M.P.Jimenez Tejada ◽  
L. F.Garcia del Moral ◽  
J. M. Ramos ◽  
F. Roca de Togores ◽  
...  
Keyword(s):  
Spring Barley ◽  
Southern Spain ◽  
Main Stem ◽  
Ear Development ◽  
Positive Correlation ◽  
Final Number ◽  
Three Stages ◽  
Developmental Phases ◽  
Barley Genotypes

SUMMARYApex and ear development in nine spring barley genotypes were studied at two sites in southern Spain in 1988. The genotypes were grouped into medium and early types, depending on the time from sowing to anthesis. Medium genotypes had significantly more spikelet primordia than early ones at both sites. Spikelet mortality was similar between genotypes and sites, resulting in a higher final number of grains/ear in the medium than the early genotypes.There was a positive correlation between the maximum number of spikelet primordia and the duration of three stages of apical development: lemma-, stamen- and awn-primordia. Negative correlations were observed between spikelet mortality and the duration of certain developmental phases of the ear.

scholarly journals DIVERSE STRATEGIES OF RHODODENDRON (Rhododendron sp.) GENOTYPES IN THE WATER SHORTAGE MANAGEMENT

2020 ◽  
Vol 19 (3) ◽  
pp. 159-165
Author(s):  
Peter Ferus ◽  
Dominika Bošiaková ◽  
Jana Konôpková ◽  
Peter Hoťka
Keyword(s):  
Climate Change ◽  
Drought Resistance ◽  
Water Shortage ◽  
Proline Accumulation ◽  
The Other ◽  
Summer Drought ◽  
Free Proline ◽  
Resistance Strategies ◽  
Relative Water ◽  
Leaf Relative Water Content

Rhododendrons in numerous gardens in Central Europe are frequently endangered by adverse summer drought periods associated with the climate change. Therefore, in this work drought-resistance strategies in recent genotypes of these highly aesthetic shrubs were investigated. Dehydrated Rhododendron groenlandicum ‘Helma’, R. obtusum ‘Michiko’ and R. hybridum ‘Polarnacht’ showed high initial stomatal conductances (gS), after few days steeply falling to the stable minimum at ca. 20, 85 and 70% leaf relative water content (RWC), respectively. Except of ‘Polarnacht’, they had relatively large specific leaf area and ‘Michiko’ also free proline accumulation. On the other hand, R. repens ‘Scarlet Wonder’ and R. hybridum ‘Red Jack’ started with half gS values, continuously declining 1.5–2 fold longer compared to the first group of genotypes (RWC of ca. 60 and 75%, respectively). Both produced relatively thick leaves but did not show any osmotic adjustment. Among observed drought-resistance strategies, lower and longer period active transpiration with stomata sensitive to the water loss, as found in R. repens ‘Scarlet Wonder’ and R. × hybridum ‘Red Jack’, were accepted as the most effective for drought-affected rhododendron plantations.

scholarly journals Response of Barley Genotypes to Fusarium Head Blight under Natural Infection and Artificial Inoculation Conditions

2021 ◽  
Vol 37 (5) ◽  
pp. 455-464
Author(s):  
Raja Khanal ◽  
Thin Meiw Choo ◽  
Allen G. Xue ◽  
Bernard Vigier ◽  
Marc E. Savard ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Prince Edward Island ◽  
Natural Infection ◽  
Spring Barley ◽  
Artificial Inoculation ◽  
High Yield ◽  
Conidial Suspension ◽  
Head Blight ◽  
One Year ◽  
Barley Genotypes

Forty-eight spring barley genotypes were evaluated for deoxynivalenol (DON) concentration under natural infection across 5 years at Harrington, Prince Edward Island. These genotypes were also evaluated for Fusarium head blight (FHB) severity and DON concentration under field nurseries with artificial inoculation of <i>Fusarium graminearum</i> by the grain spawn method across 2 years at Ottawa, Ontario, and one year at Hangzhou, China. Additionally, these genotypes were also evaluated for FHB severity under greenhouse conditions with artificial inoculation of <i>F. graminearum</i> by conidial suspension spray method across 3 years at Ottawa, Ontario. The objective of the study was to investigate if reactions of barley genotypes to artificial FHB inoculation correlate with reactions to natural FHB infection. DON concentration under natural infection was positively correlated with DON concentration (<i>r</i> = 0.47, <i>P</i> < 0.01) and FHB incidence (<i>r</i> = 0.56, <i>P</i> < 0.01) in the artificially inoculated nursery with grain spawn method. Therefore, the grain spawn method can be used to effectively screen for low DON. FHB severity, generated from greenhouse spray, however, was not correlated with DON concentration (<i>r</i> = 0.12, <i>P</i> > 0.05) under natural infection and it was not correlated with DON concentration (<i>r</i> = -0.23, <i>P</i> > 0.05) and FHB incidence (<i>r</i> = 0.19, <i>P</i> > 0.05) in the artificially inoculated nursery with grain spawn method. FHB severity, DON concentration, and yield were affected by year, genotype, and the genotype × year interaction. The effectiveness of greenhouse spray inoculation for indirect selection for low DON concentration requires further studies. Nine of the 48 genotypes were found to contain low DON under natural infection. Island barley had low DON and also had high yield.

scholarly journals Alternative screening method for drought tolerance in barley genotypes

2021 ◽  
pp. 1-5
Author(s):  
Javan Omondi Were ◽  
Julius Onyango Ochuodho ◽  
Nicholas Kipkemboi Rop ◽  
Sanjaya Gyawali
Keyword(s):  
Seed Weight ◽  
Spring Barley ◽  
Screening Method ◽  
Physiological Data ◽  
Biochemical Processes ◽  
Drought Tolerant ◽  
Randomized Design ◽  
High Yielding Varieties ◽  
Level Of Significance ◽  
Barley Genotypes

Lack of genetically stable and durable drought tolerant winter and spring barley genotypes is one of the main contributing to low and unpredictable yields in Kenya and other parts of the world despite annual release of new and high yielding varieties. Therefore, the study was set to identify genotypes exhibiting tolerance to drought through physiological and phenotypic approaches. A total of 32 genotypes were planted in split-plot arrangement in completely randomized design replicated thrice. Genotypes were maintained under 20% and 80% field capacities. Phenotypic and physiological data were collected, converted to ratios then analyzed on Genstat version 14.1 VSN International Ltd at a 5% level of significance. Significant differences were observed in winter and spring barley in terms of growth, tillering ability, grains formed per spike, 1000 seed weight and MSI (p < 0.05). Spring barley expressed higher tolerance to drought than winter barley especially in terms of height, number of grains per spike and seed weight. Water deficiency in cells and tissues might have altered and inhibited physiological and biochemical processes. The phenotypic and physiological methods corresponded and confirmed tolerance to drought in most winter and spring genotypes grown in Kenya.

scholarly journals Yield and Grain Quality of Hulless Spring Barley in Field Trials Under Different Nitrogen Management Conditions

2013 ◽  
Vol 67 (3) ◽  
pp. 229-235
Author(s):  
Māra Bleidere ◽  
Sanita Zute ◽  
Linda Brunava ◽  
Natalija Bobere ◽  
Ida Jākobsone
Keyword(s):  
Grain Yield ◽  
Crude Protein ◽  
Grain Quality ◽  
Spring Barley ◽  
Tocopherol Content ◽  
Kernel Weight ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Weight Test ◽  
Barley Genotypes

The aim of the study was to evaluate the yield and grain quality (1000 kernel weight, test weight, crude protein, starch, β-glucans, total phenolic content, antiradical scavenging activity and α-tocopherol content) variation of three hulless spring barley (Hordeum vulgare L.) genotypes under different levels of N, in relation to weather characteristics. The field experiments were carried out at the State Stende Cereal Breeding Institute. Three hulless barley genotypes ‘Kornelija’; ‘ST 1165’; ST 1185’ were studied during two years (2011 and 2012) using three levels of nitrogen (N80; N80+40; N80+80) by split application at the end of the tillering stage. The effects of genotype, N treatment and genotype by N treatment interaction contribute the variation in yield and grain quality traits for hulless barley genotypes. There were considerable differences between crop years in response of hulless genotypes to top-dressing N, with significantly (P < 0.05) higher grain yield, 1000 kernel weight, test weight, starch and β-glucans in the growing season of 2012, when rainfall was optimal at the tillering and stem-elongation growth stages. In 2012, a significant (P < 0.05) response to top-dressing N was recorded for grain yield and 1000 kernel weight (at rate N120), crude protein, starch and β-glucans (at rate N160). The maximum values of total phenolic content, antiradical scavenging activity (in 2011) and -tocopherol content (in 2012) were obtained with application of 160 kg N ha-1. Hulless line ‘1185’ gave the highest grain yield, starch, α-tocopherol content and radical scavenging activity, but variety ‘Kornelija’ formed significantly higher TKW, crude protein and β-glucans content.

scholarly journals The Heritability of Some Qualitative and Quantitative Traits at One Set of Spring Barley Genotypes

2018 ◽  
Vol 75 (2) ◽  
pp. 93-100
Author(s):  
Ioana PORUMB ◽  
Florin RUSSU ◽  
Ioan ROTAR
Keyword(s):  
Quantitative Traits ◽  
Starch Content ◽  
Spring Barley ◽  
Mean Values ◽  
Qualitative And Quantitative ◽  
Chemical Trait ◽  
The Subject ◽  
The Mean ◽  
Barley Genotypes ◽  
Collection Level

Work collections are subjected to a renewal process, therefore a regular evaluation is needed inorder to appreciate the genetic advance of the existing variability at collection level and further to identify valuable genotypes in terms of morphoproductive and qualitative traits (protein, starch). The barley intended for brewing must correspond to some qualitative parameters, of which proteins and starch content are of major importance. For this purpose, a study was conducted to assess the variability and heritability indicators corresponding to the two traits at 48 barley genotypes. The genotypes that were the subject of this study were sown in three experimental years 2013, 2014 and 2016. The mean values of 2013 year was 13.71 compared with only 10.77, and 11.27 in 2014 and 2016. The significant value of the heritability coefficient along with the genetic advantage of 0.81 indicates the success of selection work for this important chemical trait.

scholarly journals Classification of Brain Tumors Using Hybridized Convolutional Neural Network in Brain MRI images

2022 ◽  
Vol 16 ◽  
pp. 561-570
Author(s):  
V Shwetha ◽  
C. H. Renu Madhavi ◽  
Kumar M. Nagendra
Keyword(s):  
Brain Mri ◽  
Morphological Operations ◽  
Hybrid Technique ◽  
Body Images ◽  
Anisotropic Diffusion Filter ◽  
Medical Disorders ◽  
Improved Performance ◽  
Magnetic Resonance Imaging Mri ◽  
The Brain ◽  
Mri Image

In this research article, we have proposed a novel technique to operate on the Magnetic Resonance Imaging (MRI) data images which can be classified as image classification, segmentation and image denoising. With the efficient utilization of MRI images the medical experts are able to identify the medical disorders such as tumors which are correspondent to the brain. The prime agenda of the study is to organize brain into healthy and brain with tumor in brain with the test MRI data as considered. The MRI based technique is an methodology to study brain tumor based information for the better detailing of the internal body images when compared to other technique such as Computed Tomography (CT).Initially the MRI image is denoised using Anisotropic diffusion filter, then MRI image is segmented using Morphological operations, to classify the images for the disorder CNN based hybrid technique is incorporated, which is associated with five different set of layers with the pairing of pooling and convolution layers for the comparatively improved performance than other existing technique. The considered data base for the designed model is a publicly available and tested KAGGLE database for the brain MRI images which has resulted in the accuracy of 88.1%.

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