scholarly journals Alternative screening method for drought tolerance in barley genotypes

Author(s):  
Javan Omondi Were ◽  
Julius Onyango Ochuodho ◽  
Nicholas Kipkemboi Rop ◽  
Sanjaya Gyawali

Lack of genetically stable and durable drought tolerant winter and spring barley genotypes is one of the main contributing to low and unpredictable yields in Kenya and other parts of the world despite annual release of new and high yielding varieties. Therefore, the study was set to identify genotypes exhibiting tolerance to drought through physiological and phenotypic approaches. A total of 32 genotypes were planted in split-plot arrangement in completely randomized design replicated thrice. Genotypes were maintained under 20% and 80% field capacities. Phenotypic and physiological data were collected, converted to ratios then analyzed on Genstat version 14.1 VSN International Ltd at a 5% level of significance. Significant differences were observed in winter and spring barley in terms of growth, tillering ability, grains formed per spike, 1000 seed weight and MSI (p < 0.05). Spring barley expressed higher tolerance to drought than winter barley especially in terms of height, number of grains per spike and seed weight. Water deficiency in cells and tissues might have altered and inhibited physiological and biochemical processes. The phenotypic and physiological methods corresponded and confirmed tolerance to drought in most winter and spring genotypes grown in Kenya.

2016 ◽  
Vol 20 (2) ◽  
pp. 357-361
Author(s):  
J.O. Oyedeji ◽  
G.O. Imagbenikaro

The study examined the effect of cooling on the reproductive performance of gilts using 27 Large White Experimental Gilts on a Completely Randomized Design (CRD) with 3 systems of cooling, namely Wallow Cooling (WC), Sprinkler Cooling (SC) and Zero Cooling (ZC). Gilts were weighed at the commencement of the experiment and thereafter fed ad libitum from service to farrowing. Feed intake till farrow, feed cost, gilt weight at farrow, date of farrow, litter size and weight were recorded. The feed/piglet weight, feed intake/litter, feed cost/litter and birth weight/piglet were derived. The results showed that feed intake per litter and feed per piglet weight were highest for gilts placed on WC(P< 0.05), followed by SC and least for sow placed on ZC; gilt weight before farrow was highest for sow placed on SC (69.78±0.92), followed by ZC (69.33±0.94) and least for gilts placed on WC (67.33±0.87); litter sizes of gilts placed under the WC and SC systems were similar (5.6±0.41) while that under the ZC system was comparatively smaller (5.4±0.40);while the date of farrow was lowest in the ZC system (113.8±0.32) followed by SC (114.0±0.33) and highest under the WC (114.1±0.33). However, the feed intake per litter, feed per piglet weight, litter sizes of gilts, birth weight per piglet of gilts and the average date of farrow among gilts placed on WC, SC and ZC were not statistically different at 5% level of significance. Though, there is no significant difference in the performance among gilts placed on WC, SC and ZC, temperature regulation through provision of efficient cooling system in piggery would help to increase litter size, improve feed intake per litter and reduce the average cost of feeding gilt per day in maximizing reproductive performances in pigs.Keywords: Cooling, gilts, service, farrow, piggery, litter size, piglet and birth weight


Author(s):  
Amir Husein ◽  
Rudy Sutrisna ◽  
Dian Septinova

This research aimed to know the influence of giving a commercial ration with addition of local feed fermented with ammonium sulphate and urea and to find out the best ration on live weight, carcass weight, and giblet weight of male hybrid ducks. This research was carried out in July 2018--August 2018 in the cage of ducks, Department of Animal Husbandry, Faculty of Agriculture, Lampung University. Research material used consisted of 40 male hybrid ducks. This study used a Completely Randomized Design (CRD). Data was analysed using analysis of variance at 5% level of significance. The results showed that there was no different effect of treatments on live weight (1,552--1,717 g / duck), carcass weight (805,6--1.016 g / duck), and giblet weight (106.8--118.45 g /duck) of male hybrid ducks. Keywords: Carcass weight, Giblet weight, Live weight, Male hybrid duck.


2021 ◽  
Author(s):  
Jamal Abu-Elenein ◽  
Rabea Al-Sayaydeh ◽  
Zahera Akkeh ◽  
Zakaria Al-Ajlouni ◽  
AbdRaheem A. Al-Bawalize ◽  
...  

Abstract Background In barley, flowering behavior is a highly regulated and complex process where the appropriate matching of reproductive development with seasonal variation in water availability confer barley adaptation to different environments. In this study, the role of variation in flowering time and drought tolerance in four selected barley genotypes was studied under field and controlled conditions. For this purpose, field trials were conducted for two consecutive seasons at three diverse environments where the studied genotypes were subjected to either rainfed conditions or rainfed plus supplementary irrigation under two different sowing dates. Furthermore, reproductive meristem development in two selected barley genotypes, Rum (drought tolerant) and Steptoe (drought-sensitive) was also assessed in response to both vernalization and water stress under two different photoperiod conditions.Results Variation in the number of days to heading was more pronounced under rainfed conditions than under well water conditions. For agronomic performance, Rum was superior under all tested environments, which assure its general adaptability to multiple environments, while Steptoe was the poorest. The transition to reproductive meristem was faster under vernalized long-day conditions as compared to vernalized short-day conditions. The progress of shoot apical meristem development and heading under long-day conditions was significantly faster in Rum than that of Steptoe. A clear effect of drought stress was observed on shoot apical meristem development in Steptoe. Under short-day conditions, vernalized Rum plants subjected to water deficit showed an advanced meristem development stage a significant earlier HD when compared with non-stressed plants. This early flowering behavior in stressed Rum plants under short-day conditions was accompanied by higher gene expression of the Vrn-H1 gene. Conclusion In conclusion, the integration of vernalization and photoperiod signals in drought-tolerant barley genotypes is associated with early flowering behavior and higher productivity in dry environments.


2020 ◽  
Vol 22 (2) ◽  
pp. 64
Author(s):  
Krisna Cahya Nurani ◽  
Susilo Budiyanto ◽  
Endang Dwi Purbajanti

<p>Green beans are legume plants that are in great demand because of their high nutrition content and include plants that require boron to support growth from the vegetative to generative phase. The study aims to assess the growth and production of green beans due to influence of the dose and time of boron application. The research was carried out using completely randomized design with factorial pattern 3×3 with 3 replications. The first factor was the treatment of boron doses of 1, 1.5, and 2 kg/ha was given once at 7 Day After Planting (DAP), fertilizer application twice at 7 and 28 DAP, and once given at 28 DAP. The results showed that dose of boron at a dose of 1 kg/ha was able to produce the number of affective root nodules and the best pod weight. The treatment of boron once at 7 DAP can increase plant height, flowering age, pod weight, and seed weight per pod. The interaction between dose and time of boron application affects the number of affective root nodules and seed weight per pod.</p>


2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>


2021 ◽  
Vol 25 (8) ◽  
pp. 1547-1550
Author(s):  
O.A. Majekodunmi ◽  
I.O. Abiola ◽  
A.M. Aderemi ◽  
J.O. Adedipe ◽  
O.G. Ogunwale ◽  
...  

This study investigated the effect of sowing media on the seed germination and subsequent seedling growth of Senna fistula L. Treatments were T1 (Top soil), T2 (River sand), T3 (Grinded coconut husk) and T4 (weathered sawdust), replicated two times for seed germination. The treatments were arranged in a Completely Randomized Design (CRD) and the data generated was subjected to analysis of variance (ANOVA) at 5% level of significance, while mean separation where appropriate was done using Least Significant Difference (LSD). The different sowing media used had positive effect on the seed emergence, the seeds planted in river sand had the highest emergence percentage of 70%. The different sowing media used did not have any significant effect on the vegetative growth parameters, statistically, they had same effect on the parameters measured. Nonetheless, seedlings from river sand produced highest number of leave (20.4), seedlings from topsoil produced highest plant height and largest leaf area of l0.62 cm and 37.l4 cm2 respectively. The seedlings from weathered sawdust produced the highest stem diameter of 0.79 mm, while seedlings from grinded coconut husk had lowest values for all the growth parameters measured. Therefore, it is recommended that river sand or weathered sawdust can be used to propagate Senna fistula seeds.


2011 ◽  
Vol 9 (2) ◽  
pp. 38-44
Author(s):  
HADI WIYOTO ◽  
M. A.M. ANDRIANI ◽  
NUR HER RIYADI PARNANTO

Wiyoto H, Andriani MAM, Parnanto NHR. 2011. Study of antioxidant activity and anti-cholesterol content on red yeast rice with substrates variation (rice, corn and dried cassava). Biofarmasi 9: 38-44. Red yeast rice is one of fermented rice product by Monascus purpureus. Traditionally, the substrate used to produce red yeast rice is rice. Usually, the rice with high amylose content is proper to produce red yeast rice than low amylose. The other substrates that be used to produce red yeast rice are corn and dried cassava. The purposes of this research were to determine the effect of substrates variation (rice, corn and dried cassava) on antioxidant activity and anti-cholesterol content in red yeast rice, and to determine the substrate(s) that produce the highest antioxidant activity and anticholesterol content. The design of this research was a Completely Randomized Design with one factor, i.e. the kind of substrates: rice, corn and dried cassava, with three replications. Then, the data were analyzed with ANOVA at a level of significance α=0.05, and continued with DMRT at the same level. This results showed that the effect of substrates kind to antioxidant activity and anti-cholesterol content on red yeast rice. The rice substrate had higher antioxidant activity and anti-cholesterol content than corn and dried cassava substrates. The antioxidant activity and the anti-cholesterol content on red yeast rice from rice substrate were 45.6100% and 0.026600%, respectively. The antioxidant activity and the anti-cholesterol content on red yeast rice from corn substrate were 44.0500% and 0.022833%, respectively, while the antioxidant activity and the anti-cholesterol content on red yeast rice from dried cassava substrate were 42.8333% and 0.013200%, respectively.


2018 ◽  
Vol 98 (4) ◽  
pp. 619-630 ◽  
Author(s):  
D. Kumar ◽  
C. Raginski ◽  
K. Schwean-Lardner ◽  
H.L. Classen

Ideally balanced dietary protein is critical for laying hen egg production and feed efficiency, but also affects other important characteristics. This research was designed to study the nonegg production and feed intake response of Lohmann-LSL Lite hens to 550, 625, 700, 775, and 850 mg d−1 of amino acid balanced digestible lysine (Dlys) from 27 to 66 wk of age. Data collection included hen weight (HW), feather scoring, tissue weights, egg specific gravity, egg component weights, and excreta nitrogen (N) content. The experiment was a completely randomized design and level of significance was fixed at P ≤ 0.05. Hen weight (quadratic, Q), pectoralis muscle (absolute, % – Q), and abdominal fat (absolute – linear, L; % – Q) increased with increasing Dlys intake. Although gastrointestinal segment weights and lengths were affected by Dlys intake, interpretation of results was confounded by other aspects of diet composition. Hen feather score (L) and excreta N content (Q) increased, and egg shell quality (L) decreased with increasing Dlys intake. Absolute egg component weights increased with Dlys intake, but effects on proportional weights were Q and relatively minor. In conclusion, balanced Dlys intake affected a variety of practical nonproduction characteristics in laying hens.


2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 192-192
Author(s):  
Ludovic Lahaye ◽  
Glenmer Tactacan ◽  
Seung-Yeol Cho ◽  
Jinho Cho ◽  
In Ho Kim ◽  
...  

Abstract Volatility in feed ingredient prices prompts animal nutritionists to evaluate alternative methods to control feed costs. The objective of this study was to evaluate the effects of protease in growing pigs fed either high or low-density diets. A total of 140 pigs [(Landrace×Yorkshire)×Duroc] were used in a 6-wk study with an initial BW of 24.1 ± 0.02 kg equally distributed in 7 pens per treatment fed one of the following treatments: High-density diet with 3400 kcal ME/kg, 19.5% CP, and 0.85% SID Lys; High-density diet + 125 g/t Jefo Protease (Jefo, Canada); Low-density diet with 3300 kcal ME/kg, 17.6% CP, and 0.83% SID Lys; and Low-density diet + 125 g/t Jefo Protease. Diets were corn, soybean meal-based with 12% rice bran and 8% wheat bran. Data were subjected to statistical analyses as a completely randomized design using a 2 × 2 factorial arrangement with pen as the experimental unit. Differences among treatment means were determined using Duncan’s multiple range test with level of significance at P ≤ 0.05. High-density diets (P = 0.01) and protease supplementation (P = 0.05) significantly improved G:F in pigs (Table 1) compared to low-density diets and no protease supplementation. NH3 and H2S gas emission tended to be lower (P ≤ 0.10) in diets supplemented with protease. There were no statistical differences (P > 0.10) in initial weight, final weight, ADG, and ADFI. In conclusion, protease supplementation and high density diets improved G:F in 25- to 55-kg pigs.


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