scholarly journals Quantitative Analysis of UV-B Radiation Interception and Bioactive Compound Contents in Kale by Leaf Position According to Growth Progress

2021 ◽  
Vol 12 ◽  
Author(s):  
Hyo In Yoon ◽  
Hyun Young Kim ◽  
Jaewoo Kim ◽  
Jung Eek Son
Keyword(s):  
Bioactive Compounds ◽  
Bioactive Compound ◽  
Flavonoid Compound ◽  
Total Phenolic ◽  
Photosynthetic Parameters ◽  
Leaf Position ◽  
Plant Structure ◽  
Radiation Interception ◽  
Structure Changes ◽  
Controlled Environments

UV-B (280–315 nm) radiation has been used as an effective tool to improve bioactive compound contents in controlled environments, such as plant factories. However, plant structure changes with growth progress induce different positional distributions of UV-B radiation interception, which cause difficulty in accurately evaluating the effects of UV-B on biosynthesis of bioactive compounds. The objective of this study was to quantitatively analyze the positional distributions of UV-B radiation interception and bioactive compound contents of kales (Brassica oleracea L. var. acephala) with growth progress and their relationships. Short-term moderate UV-B levels did not affect the plant growth and photosynthetic parameters. Spatial UV-B radiation interception was analyzed quantitatively by using 3D-scanned plant models and ray-tracing simulations. As growth progressed, the differences in absorbed UV-B energy between leaf positions were more pronounced. The concentrations of total phenolic compound (TPC) and total flavonoid compound (TFC) were higher with more cumulative absorbed UV-B energy. The cumulative UV energy yields for TFC were highest for the upper leaves of the older plants, while those for TPC were highest in the middle leaves of the younger plants. Despite the same UV-B levels, the UV-B radiation interception and UV-B susceptibility in the plants varied with leaf position and growth stage, which induced the different biosynthesis of TFC and TPC. This attempt to quantify the relationship between UV-B radiation interception and bioactive compound contents will contribute to the estimation and production of bioactive compounds in plant factories.

Improvement of bioactive compounds content in granadilla (Passiflora ligularis) seeds after solid-state fermentation

2020 ◽  
pp. 108201322094400
Author(s):  
Tacila RJ Santos ◽  
Paula RB Feitosa ◽  
Nayjara C Gualberto ◽  
Narendra Narain ◽  
Luciana CLA Santana
Keyword(s):  
Solid State ◽  
Gallic Acid ◽  
Bioactive Compounds ◽  
Solid State Fermentation ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Seed Flour ◽  
Pharmaceutical Industries ◽  
Acetone Extracts ◽  
Gallic Acid Equivalent

Fermentation improves the bioactivity of fruit by-products; therefore, this study aimed to increase the bioactive compound content in granadilla ( Passiflora ligularis) seed flour (with 50% and 70% initial moisture) through solid-state fermentation using the fungus Aspergillus niger. The extracts were obtained with distilled water, 40% acetone, 80% acetone, 40% ethanol, or 80% ethanol. The highest total phenolic (4713.3 of gallic acid equivalent/100 g of granadilla seed flour in dry basis) and total flavonoid (1910.4 mg of quercetin/100 g of granadilla seed flour in dry basis) contents were obtained with granadilla flour at 50% initial moisture fermented for 48 h with 80% acetone extractor solvent. The highest antioxidant activity was obtained with 80% acetone from flour fermented for 168 h. The chromatographic analysis showed 10 compounds identified in the 80% acetone extracts of fermented flour; gallic acid and epigallocatechin were the major compounds. Gallic acid, catechin, 6,2′-di-hydroxyflavone, ethyl gallate and coumarin had higher concentrations in extracts of fermented flours when compared with unfermented ones. Only fermented flours showed the presence of protocatechuic acid compound. The solid-state fermentation was efficient to obtain extracts of granadilla seed flour enrichment of antioxidant bioactive compounds with potential of application in food, cosmetic and pharmaceutical industries.

Effects of thermal pasteurization on jambolan tropical juice bioactive compounds

2019 ◽  
Vol 121 (11) ◽  
pp. 2821-2834
Author(s):  
Gilciane Américo Albuquerque ◽  
Ana Vânia Carvalho ◽  
Lênio José Guerreiro de Faria ◽  
Renan Campos Chisté ◽  
Luiza Helena da Silva Martins ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Statistical Significance ◽  
Bioactive Compound ◽  
Enzymatic Browning ◽  
Total Phenolic ◽  
Optimal Point ◽  
Total Anthocyanin ◽  
Content Type ◽  
Activity Of Enzymes

Purpose Jambolan has be used as a diabetes-treatment aid, and it has become quite popular; studies have been carried out over the years on this fruit because it presents high activity against superoxide anion and hydroxyl radicals due to the bioactive compounds that may play an important role against oxidative stress. No studies have been reported on the effects of pasteurization on the bioactive compounds of jambolan tropical juice. The paper aims to discuss these issues. Design/methodology/approach For evaluation of the effects of pasteurization on jambolan juice bioactive compounds, a complete compound and rotational experimental design was carried out by varying temperature (80–90°C) and time (45–75 s). The bioactive compounds (total phenolic, total anthocyanin and antioxidant activity) and the enzymatic activity of enzymes (enzymatic browning) were assessed. Findings The study of bioactive compounds in the pasteurized juice of jambolan, showed that the total phenolics (TP) and ABTS antioxidant activity presented statistical significance (p<0.05). The action of enzymes causing enzymatic browning was only observed in the standard sample. The optimization of the pasteurization process with the maximum bioactive compound retention was 91.65°C for 69.45 s. The optimal point found is in agreement with studies on thermal treatment that recommend using short times at high temperatures for better retention of food constituents. Originality/value The incidence of chronic diseases in humans has increased significantly over the years, some bioactive phytochemicals present in fruits may help against these diseases, so the production of tropical jambolana juices can help to nourish and regulate the consumer health. Therefore, studying the maintenance of bioactive compounds present in this product could be an alternative for the production of healthier foods.

Bioactive compounds by UPLC-PDA in different cocoa clones (Theobroma cacao L.) developed in the Southern region of Bahia, Brazil

2017 ◽  
Vol 119 (9) ◽  
pp. 2117-2127 ◽  
Author(s):  
Leonardo Fonseca Maciel ◽  
Ana Lúcia de Souza Madureira Felício ◽  
Elisa Yoko Hirooka
Keyword(s):  
Bioactive Compounds ◽  
Phenolic Content ◽  
Principal Component ◽  
Bioactive Compound ◽  
Ultra Performance Liquid Chromatography ◽  
Southern Region ◽  
Cocoa Bean ◽  
Total Phenolic ◽  
Array Detector ◽  
Content Type

Purpose Many factors can influence the bioactive compounds in cocoa beans. The purpose of this paper is to characterize the bioactive compound profile of 12 cocoa bean clones produced in Bahia’s Southern region. Design/methodology/approach Total phenolic content, total flavonoids and total anthocyanins were determined using spectrophotometric methods. Caffeic acid, caffeine, theobromine, catechin and epicatechin contents were determined using Ultra Performance Liquid Chromatography with a Photodiode Array Detector. Principal component analysis (PCA) was carried out using to determine the variability of the results found in relation to the clones studied. Findings The authors observed variations in the bioactive compound content between the analyzed cocoa clones. The SRN clone showed the highest levels of phenolic content, flavonoids, caffeine, catechin, and epicatechin. PCA showed that all the clones had unique characteristics related to the composition of their bioactive compounds content, classifying each clone in a well-defined manner. Originality/value This work is an important contribution for the characterization of new cocoa bean clones in relation to their content of bioactive compounds. The information reported in this paper can be decisive in the choice of the variety of cocoa in the production of chocolate with high bioactive compounds content and claim of functional property.

scholarly journals The Chemical Fermentation Process Properties, Bioactive Compounds, and Health Benefits of Fruit Vinegars in Pilot-Scale in Thailand

2021 ◽  
Vol 18 (4) ◽  
Author(s):  
Wilawan BOONSUPA
Keyword(s):  
Acetic Acid ◽  
Bioactive Compounds ◽  
Fermentation Process ◽  
Raw Materials ◽  
Health Benefits ◽  
Liquid Product ◽  
Chemical Properties ◽  
Bioactive Compound ◽  
Pilot Scale ◽  
Total Phenolic

Vinegar is a liquid product produced from alcoholic and acetous fermentation. Vinegar contains acetic acid and bioactive compounds, which are brewed by liquid-state and solid-state fermentation techniques. This study reviews chemical fermentation process properties, bioactive compounds, and health benefits of fruit vinegars in Thailand. Chemical properties are the alcohol percentage and acetic acid percentage of vinegar. Bioactive compounds include antioxidant activity and total phenolic content, which have the role of antioxidative activity, blood pressure and glucose control, and anti-tumor. However, further studies are needed to find the new fruit raw materials to produce fruit vinegar which have more bioactive compound and more good taste.

scholarly journals Impact of refrigerated storage on the bioactive compounds and antioxidant capacity of two Algerian carrot varieties (Daucus carota L.)

2020 ◽  
Vol 13 (1) ◽  
pp. 5-31
Author(s):  
L. Arkoub-Djermoune ◽  
H. Louaileche ◽  
F. Benmeziane ◽  
K. Madani ◽  
L. Boulekbache-Makhlouf
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Daucus Carota ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Daucus Carota L

Abstract Carrot (Daucus carota L.) is one of the main root vegetables rich in bioactive compounds with appreciable health-promoting properties, largely consumed in Algeria. In the current study, the storage effect (at 4 °C throughout 12 days) on bioactive compound stability and the antioxidant activity of two Algerian orange carrot varieties (Supermuscade and Touchon) were investigated. Total phenolic content of samples was determined by the Folin–Ciocâlteu method. Antioxidant capacity was determined spectrophotometrically, based on the evaluation of Free Radical Scavenging Activity (FRSA) using DPPH radical and Ferric Reducing Power (FRP). The results showed that the Touchon variety is richer in phenolics, flavonoids, and carotenoids and presents higher antioxidant activity in comparison with the Supermuscade variety. At the end of storage, the bioactive compound content and antiradical activity increased significantly (p < 0.05). Also, an extremely significant correlation (p < 0.001) was observed between the antioxidant contents and the antioxidant capacities of aqueous carrot extracts.

scholarly journals Determination of Phenolic Composition and Antioxidant Activity in Flowers and Stems of Rumex Tunetanus Using RP-UHPLC-ESI-QTOF-MS

2018 ◽  
Author(s):  
Jouda Abidi ◽  
Sonda Ammar ◽  
Mohamed Bouaziz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Selective Extraction ◽  
Total Phenolic ◽  
Negative Ionization Mode ◽  
Negative Ionization

The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in Gar&acirc;aSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r &gt; 0.86. The compounds displaying significant (P &lt; 0.01) and good correlations with the antioxidant activity (r &gt; 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus. Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.

Structural Modification of Styrene Maleic Anhydride Copolymers for Plant Bioactive Compound Extraction

2019 ◽  
Vol 798 ◽  
pp. 351-357
Author(s):  
P. Punyamoonwongsa ◽  
Pattana Kakumyan ◽  
Natsaran Saichana ◽  
Brian Tighe
Keyword(s):  
Maleic Anhydride ◽  
Phase Diagrams ◽  
Bioactive Compounds ◽  
Ternary Phase ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Ph Change ◽  
Extraction Technology ◽  
Membrane Solubilization ◽  
Ternary Phase Diagrams

Ability of poly (styrene-alt-maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α-helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive compounds are recovered from plant tissues for different reasons. One of the most important reasons is due to the increased demand in nutraceuticals market and modern therapeutics. Despite this, aqueous-based extraction of these compounds has been reported to give low extraction yield. A development of new green extraction protocol is still a challenging task for all researchers nowadays. This study demonstrated, for the first time, possible use of PSMA as a lysis agent for plant bioactive compound extraction. To enhance its membrane affinity at physiological pH, the polymer was esterified with methanol. Both PSMA and its derivative (ePSMA) were characterized in terms of their membrane binding affinity through a combined use of both surface characterization and physical techniques. Analysis of the ternary phase diagrams suggested that ePSMA could facilitate stronger hydrophobically-driven interactions with the lipid. This was convinced by the reduced critical PSMA/lipid mass ratio from 7:1 (PSMA) to 1:1 (ePSMA), as observed in the ternary phase diagrams. Last but not the least, the crude extracts of Coffea robusta leaves obtained from ePSMA-based extraction showed a total phenolic content of 20.32±0.75 mg/g sample, significantly higher than that from the PSMA- (14.24±1.27 mg/g sample) and aqueous-based (16.33±1.03 mg/g sample) extractions. A structural manipulation of PSMA is thus a key to tailor its membrane solubilization and so, the extraction efficacy of bioactive compounds from plant cells.

scholarly journals Enhancement of antioxidant activity and bioactive compound contents in yellow soybean by plant-extract-based products

2019 ◽  
Vol 71 (3) ◽  
pp. 425-434
Author(s):  
Sanja Djurovic ◽  
Vesna Dragicevic ◽  
Hadi Waisi ◽  
Maja Pagnacco ◽  
Nevena Lukovic ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Plant Extracts ◽  
Technological Development ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Ministry Of Education ◽  
Cell Organelles ◽  
Education Science

Polyphenols present in different plant cell organelles increase the resistance of plants to various types of environmental stresses. We investigated the possibility of increasing the content of bioactive compounds in the seed of yellow soybean variety Laura. The soybean was treated during vegetation with five products based on plant extracts, on the assumption of enrichment of plants with various nutrients. Soybean flour extracts were screened spectrophotometrically for total phenolic content and antioxidant activity. The antioxidant activity was evaluated using three methods. The content of phenolic acids was determined by HPLC, and the raw protein content was estimated by the Kjeldahl method. Depending on the treatment, variations in the quantity of individual phenolic acids with up to 90% higher concentration as compared to the control were observed. Controlled usage of certain plant extracts can increase the concentration of the target group of bioactive compounds in the samples. The synergistic effect of proteins and phenolic compounds on the antioxidant activity of extracts was detected. The results of this study are not only important from the aspect of plant resistance to various types of stress, but also when considering soybean as a functional food. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR 31018, Grant no. 31037, Grant no. 37021 and Grant no. 172015]

scholarly journals Bioactive Compound Contents and Antioxidant Activity of of Fermented Lead Tree (Leucaena leucocephala (lmk.) de Wit) Seeds

Molekul ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 194
Author(s):  
Venty Suryanti ◽  
Soerya Dewi Marliyana ◽  
Gracia Lasma Rohana ◽  
Elyna Wahyu Trisnawati ◽  
Widiyanti Widiyanti
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Petroleum Ether ◽  
Bioactive Compounds ◽  
Leucaena Leucocephala ◽  
Bioactive Compound ◽  
Total Phenolic ◽  
Tree Seeds ◽  
Β Carotene ◽  
Tree Seed

Leucaena leucocephala (lmk.) de Wit) (leed tree) seeds have been fermented by Indonesian as a traditional food which is called tempeh. The fermentation products of legume seeds are good functional foods, since they contain many bioactive compounds. The objective of this study was to determine the bioactive compound contents of fermented lead tree seed, such as total phenolic, ascorbic acid, β-carotene and a-tocopherol. Antioxidant activity testing was carried out using the thiobarbituric acid reactive substances (TBARS) method. Analysis of total phenolics, ascorbic acid, β-carotene and a-tocopherol were carried out using the folin-ciocalteu method, iodine titration, spectrophotometry and iron (III) chloride-thiocyanate, respectively. Rhizopus oligosporus was used to ferment lead tree seeds for 0, 1, 2, 3 and 4 days. Fermented leed tree seed powder was extracted with petroleum ether followed by ethanol. In comparison to unfermented leed tree seed powder, the β-carotene and a-tocopherol of the fermented seed powder increased. The greatest enhancement of β-carotene and a-tocopherol contents were observed at day 2 of fermentation. The highest antioxidant activities of fermented leed tree seeds were also obained at day 2 of fermentation with 55.55±4.59 and 74.46±3.49% inhibition in petroleum ether and ethanol extracts, respectively. The results show that lead tree seed tempeh has high antioxidant activity due to the bioactive compounds contains, such as  phenolics, β-carotene and a-tocopherol. Therefore, leed tree seed tempeh could be used for possible commercial production of functional food to alleviate oxidative stress.

scholarly journals Effect of lactic fermentation, microwave, and ultrasonic extraction on the bioactive compounds from Anoectochilus formosanus Hayata

Food Research ◽  
2021 ◽  
Vol 5 (5) ◽  
pp. 287-293
Author(s):  
M.D. Lieu ◽  
T.K.T. Dang
Keyword(s):  
Bioactive Compounds ◽  
Fermentation Process ◽  
Bioactive Compound ◽  
Perennial Herb ◽  
Total Phenolic ◽  
Individual Treatment ◽  
Fermentation Time ◽  
Lactic Fermentation ◽  
Significant Difference ◽  
Anoectochilus Formosanus

Anoectochilus formosanus (family Orchidaceae) is a perennial herb that contains many bioactive valuable. In this study, the extract efficiency of bioactive compounds from Anoectochilus formosanus Hayata by microwave-assisted extraction (MAE), ultrasonicassisted extraction (UAE) treatments, and lactic fermentation in individual and combined impacts were evaluated through the total phenolic content, total polysaccharide content, and antioxidant activity. The results showed that MAE and UAE treatments and lactic fermentation enhanced the effective extraction of bioactive compounds compared to the control samples. The bioactive compound contents from A. formosanus fluid trend differences in individual treatment factors. However, there was no significant difference between these treatment factors in which the fermentation process requires more time to reach the expected extraction efficiency. The ultrasonic pretreatment combined lactic fermentation process would bring many benefits; first, no need for more the MAE treatment; second, reduce fermentation time; third, bring a probiotic source as well as valuable metabolic products from the lactic fermentation process.

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