scholarly journals Profiling of Phenolic Compounds Composition, Morphological Traits, and Antioxidant Activity of Miscanthus sacchariflorus L. Accessions

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 243 ◽  
Author(s):  
Bimal Kumar Ghimire ◽  
Erik J. Sacks ◽  
Seung Hyun Kim ◽  
Chang Yeon Yu ◽  
Ill Min Chung

Miscanthus sacchariflorus is a potential source of sustainable biofuel and other bioactive compounds. The high adaptive range of M. sacchariflorus may cause variation in its morphological traits and phytochemical composition. Although some metabolites have been reported from M. sacchariflorus, little is known about its phenolic compound composition and antioxidant or oxidant properties. This study evaluated the morphological traits, antioxidant properties, and phenolic compound profile of M. sacchariflorus collected from various regions of China, Korea, Japan, and Russia. The antioxidant potential of the leaf extracts of various accessions of M. sacchariflorus was estimated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS). An extensive study of the antioxidant activity and phenolic compounds of M. sacchariflorus obtained from different locations in four different countries could provide a comprehensive catalogue of the phytoconstituents and antioxidant properties of M. sacchariflorus accessions to consumers and nutraceutical industries. A total of 22 phenolic compounds were identified and quantified, among which p-coumaric acid, ferulic acid, p-hydroxybenzoic acid, chlorogenic acid, vitexin, and luteolin were the most dominant phytochemical compounds detected in the majority of the accessions. The antioxidant potential (DPPH) of the leaf extracts of all of the accessions ranged from 28.85 ± 1.53 µg mL−1 in MS-447 to 99.25 ± 1.63 µg mL−1 in MS-190. The antioxidant properties (ABTS) of the leaf extracts of all accessions ranged from 25.65 ± 2.06 in MS-258 to 83.62 ± 2.02 in MS-271. Pearson’s correlation analysis showed a significant and positive correlation between antioxidant activity and total phenolic content, and total flavonoid content varied widely among M. sacchariflorus accessions from the four geographical study regions. A strong and positive association was observed between DPPH with total phenolic content and total flavonoid content. Moderately positive correlations were observed between DPPH scavenging activity with gentisic acid, p-hydroxybenzoic acid, chlorogenic acid, p-coumaric acid, rutin, and quercetin (r = 0.385, r = 0.379, r = 0.362, r = 0.353, r = 0.490, and r = 0.372, respectively), suggesting that phenolic compounds are major contributors to the antioxidant potential of M. sacchariflorus. Thirty-two accessions collected from four different countries (China, Korea, Japan, and Russia) were characterized for 17 quantitative morphological traits. A wide range of diversity was observed in the morphological traits, with plant height ranging from 18.00 ± 1.00 cm to 163.20 ± 4.00 cm. Plant height was significantly correlated with biomass yield (fresh weight; r = 0.439, p < 0.05) and also had moderately positive correlations with culm length (r = 0.356, p < 0.05). Culm length was moderately correlated with the biomass yield fresh weight (r = 0.419*, p < 0.05) and the biomass yield dry weight (r = 0.425*, p < 0.05); however, it exhibited weak and negative correlations with compressed plant circumference (CCirc) (r = −0.374, p < 0.05) and total culm node number (TCmN) (r = −0.440, p < 0.05). Principal components analysis was performed to assess the variation in 17 morphological traits in 32 accessions of M. sacchariflorus. The first two principal components explained 51.24% of the morphological variations. A dendrogram generated from unweighted pair group method with arithmetic mean (UPGMA) clustering based on morphological characters was not found to be consistent with another dendrogram based on phytochemicals. In both cases, the number of studied accessions collected from different geographical regions grouped into two major groups. However, no clear correlation between these two different approaches was found. The substantial variation in the morphological traits, bioactive properties, and phenolic compounds among the accessions may provide useful information for breeding programs attempting to obtain M. sacchariflorus varieties with improved phenolic compounds traits and improved bioactive properties.

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Francisco Erik González-Jiménez ◽  
Juan Alfredo Salazar-Montoya ◽  
Graciano Calva-Calva ◽  
Emma Gloria Ramos-Ramírez

Due to their antioxidant properties, polyphenolic compounds are considered beneficial for human health. In this work, we investigated the polyphenol profile and antioxidant activity of edible tejocote (Crataegus pubescens) fruit extracts by micellar electrokinetic chromatography (MEKC) and HPLC/UV. The major phenolic compounds in the pulp extracts were (+)-catechin (9.17 ± 0.20 mg/100 mg dry fruit), (−)-epicatechin (4.32 ± 0.11 mg/100 mg dry fruit), and chlorogenic acid (5.60 ± 0.24 mg/100 mg dry fruit). The total phenolic content was 168.6 ± 0.9 mg gallic acid equivalent/g dry fruit; the total proanthocyanidin content was 84.6 ± 1.4 mg cyanidin/100 g dry fruit; and the total flavonoid content was 55.89 ± 1.43 mg quercetin/g dry fruit. Interestingly, procyanidins (dimers, trimers, and tetramers of (−)-epicatechin and (+)-catechin) were detected in the extract. This is the first study reporting the presence of polymeric polyphenols in Crataegus pubescens fruit. Accordingly, these fruits demonstrate great potential as a natural source of antioxidant phenolic compounds and could therefore be used as a nutraceutical and functional food.


2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammad Hosein Farzaei ◽  
Mahnaz Khanavi ◽  
Ghazaleh Moghaddam ◽  
Farzaneh Dolatshahi ◽  
Roja Rahimi ◽  
...  

Tragopogon graminifoliusDC. (TG), Compositae family, is traditionally used for the treatment of various diseases like gastrointestinal and hepatic disorders. The aim of the present study is to standardize extracts from TG used for preparation of different dosage forms in traditional Iranian medicine (TIM) based on phenolic compounds. For this purpose, total phenolic content and some phenolic compounds were determined in ethanolic extracts from aerial part and root of TG by HPLC method. Furthermore, antioxidant activity was evaluated using DPPH-HPLC methods. Caffeic acid, gallic acid,ρ-coumaric acid, ferulic acid, and catechin were detected in root and aerial part of TG.ρ-Coumaric acid (6.357 ± 0.014 mg·g−1) was dominant phenolic compound in aerial part followed by ferulic acid (1.24 ± 0.018 mg·g−1). Also,ρ-coumaric acid (2.685 ± 0.031 mg·g−1) was highly abundant in root, followed by catechin (2.067 ± 0.021 mg·g−1). Antioxidant activity of root extract (460.45 ± 0.78 µg Vit.E.E·mL−1) was better than that of aerial part. Generally, phenolic compounds are one of the major constituents of TG and could be used as markers for standardization of dosage forms prepared from this plant. Also, TG demonstrated significant antioxidant activity using DPPH-HPLC method. Phenolic compounds of TG may be responsible for its marked antioxidant properties.


Author(s):  
Jouda Abidi ◽  
Sonda Ammar ◽  
Mohamed Bouaziz

The present study was designed to investigate the bioactive compound in Rumextunetanus extracts (polygonaceae), a plant growing in Gar&acirc;aSejnane region (NW-Tunisia). Hydro-methanol extracts of flowers and stems of Rumextunetanus were analyzed by RP-UHPLC-ESI-QTOF-MS in the negative mode to identify the maximum of bioactive compounds. Applied the aforementioned method, a total of 60 bioactive compounds were characterized for the first time in Rumextunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. Quantification of the identified compounds revealed that quercetin-3-O-glucuronide and (-)-epicatechingallate were the most abundant phenolic compounds in flowers and stems, respectively. Moreover, positive correlations were found between the antioxidant activity measured by DPPH and FRAP assays with the total phenolic compounds (r = 0.98; r = 0.99, respectively) and the abundance of some phenolic subfamilies such as hydroxycinnamic acids, hydroxybenzoic acids, flavonols and flavones with r &gt; 0.86. The compounds displaying significant (P &lt; 0.01) and good correlations with the antioxidant activity (r &gt; 0.93) were hydroxybenzoic acid, rutin, quercetin-3-O-glucuronide, quercetin-3-O-glucoside, quercetin and luteolin-7-O-rutinoside. In addition, the flowers and stems of Rumex tunetanus showed different bioactive compound profiles and significant antioxidant properties of extracts. These results highlight the potential of the RP-UHPLC-ESI-QTOF-MS and MS/MS system to identify untargeted metabolic profiling of Rumex tunetanus. Overall, these results contribute to the clear explanation of the past and current usage of genus Rumex in folk medicine. Future investigations are necessary to develop purified antioxidant extracts, with the application of more selective extraction techniques.


2023 ◽  
Vol 83 ◽  
Author(s):  
T. S. Neri ◽  
K. W. L. Silva ◽  
L. P. S. Maior ◽  
S. K. Oliveira-Silva ◽  
P. V. M. Azevedo ◽  
...  

Abstract Croton argyrophylloides Muell. Arg., from the Euphorbiaceae family, popularly known as marmeleiro prateado or sacatinga, is a plant from the Caatinga biome commonly found in Brazil’s northeastern region. The present study aimed to evaluate the antioxidant activity of the species. The phytochemical study was performed through qualitative analysis of chemical constituents and quantitative determination of the total phenol content through the Folin-Ciocalteu test. The qualitative and quantitative antioxidant tests were performed using the DPPH method (2.2 diphenyl-1-picryl hydrazil) and ferric reducing antioxidant power (FRAP). The minimum inhibitory concentration (MIC) was determined by microdilution in 96-well plates. The ethanolic extract of the leaves of C. argyrophylloides manifested antioxidant action in the quantitative DPPH test with a significant bioactivity of 84.70 AAO% in 500 µg/mL, with an EC50 of 236.79. The content of total phenolic compounds was 946.06 mg of gallic acid equivalents/g of sample, and total flavonoids was 58.11 mg of quercetin equivalents/g of sample, the result obtained for FRAP was 15294.44 µM Trolox/g of sample and ABTS was 718 μM Trolox of sample. The prospecting of the chemical constituents of the leaves of C. argyrophylloides revealed the presence of the main compounds that manifests the antioxidant activity and it was proven by the DPPH method that there is antioxidant activity in the analyzed sample, in addition to demonstrating a significant content of phenolic compounds and total flavonoid content in the species, which corroborates the antioxidant activity of the plant sample. The leaf extracts presented growth inhibition halos of 10 and 12 mm upon Staphylococcus aureus ATCC 25923.


Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3082 ◽  
Author(s):  
Milena Polumackanycz ◽  
Tomasz Sledzinski ◽  
Elzbieta Goyke ◽  
Marek Wesolowski ◽  
Agnieszka Viapiana

Morus alba L. (white mulberry) has been commonly used as a functional food and for medicinal purposes. Hence, the aim of the study was to compare the phenolic profile of white mulberry commercial samples in relation to their antioxidant potential and acetylcholinesterase (AChE) inhibitory activity. It is of interest to determine whether herbal products originating from different commercial sources differ in their phenolic profiles. For this purpose, a simple and rapid high-performance liquid chromatography (HPLC) method was used for the separation and determination of ten major phenolic compounds. Total phenolic (TPC), total flavonoid (TFC), and total phenolic acid contents (TPAC), as well as l(+)-ascorbic acid (ASA) contents, were determined. The antioxidant potential was assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl radical) scavenging activity and ferric-reducing/antioxidant power (FRAP) assay, while the AChE inhibitory activity was determined by the Ellman assay for water extracts. The study revealed that excluding two herbal products containing fruits and a sample containing leaves of white mulberry, yerba mate and lemon, the remaining samples were generally consistent in terms of phenolic composition as well as antioxidant potential and AChE inhibitory activity. This reflects the health-promoting properties of the samples under study. Moreover, the results showed that the water extracts of white mulberry were richer in phenolic compounds and presented higher antioxidant activity than the hydromethanolic extracts. However, the water extracts showed low inhibitory activity against AChE. Moreover, the correlation analysis indicated a high positive relationship between phenolic composition and antioxidant activity in extracts of white mulberry. Overall, the obtained results may be useful in the evaluation of new dietary supplements and food products. The water extracts of white mulberry could be used for antioxidant purposes, while the hydromethanolic extracts could be incorporated in antioxidant formulations.


2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mohd Aaqib Sheikh ◽  
Charanjiv Singh Saini ◽  
Harish Kumar Sharma

PurposeThe study was aimed to explore the potential impact of microwave heating (450 W for 2, 4, 6 and 8 min) on antioxidant activity, anti-nutritional factors, volatile and phenolic compounds of the plum kernels.Design/methodology/approachPlum kernels are rich in proteins, lipids and bioactive compounds that are mostly underused and undervalued.FindingsThe results showed that microwave heating at 450 W for 6 min significantly (p < 0.05) increased the antioxidant activity, total phenolic and flavonoid content, while the longer treatment time (450 W for 8 min) adversely affected the phenolic compounds. Most importantly, the anti-nutritional factors like amygdalin, hydrocyanic acid, phytic acid and tannin content were reduced up to 87.1, 84.7, 20.9 and 46.2%, respectively at 450 W for 6 min treatment conditions, which was confirmed from the larger shifts observed in FT-IR spectra near 1,157 cm−1. Microwave heating at 450 W for 6 min also proved beneficial in improving the bioavailability of volatile and phenolic compounds including chlorogenic acid, gallic acid, syringic acid, (+)-catechin, caffeic acid, ß-carotene, trans-ferulic acid, rutin trihydrate, 3,4-dihydroxybenzoic acid, tannic acid and quercetin by liberating them from the plant matrix.Originality/valueThe results thus indicate that controlled microwave heating could be an effective approach for the reduction of anti-nutritional factors besides leading to an overall improvement in antioxidant potential and volatile and phenolic compounds. This novel technological approach can proliferate the use of plum kernels in different diversified food formulations.


Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 715
Author(s):  
Cindy Dias ◽  
Alexandre M. A. Fonseca ◽  
Ana L. Amaro ◽  
Ana A. Vilas-Boas ◽  
Ana Oliveira ◽  
...  

Fruit enzymatic browning (EB) inhibition continues to be a challenge in the Food Industry. This physiological disorder results mainly from the oxidation of natural phenolic compounds by polyphenoloxidase (PPO) and peroxidase (POX) leading to the formation of brown pigments. EB can be controlled with the application of antioxidants, reducing/inhibiting the activity of these oxidative enzymes. In this study, strawberry tree (leaves and branches) and apple byproduct were the natural-based extracts (NES) selected, as potential tissue browning inhibitors, within a first screening of fifteen natural-based extracts with antioxidant properties. Phenolic profile, total phenolic content and antioxidant activity of the selected extracts were also performed as well as their depletion effect on the oxidative enzyme’s activity and browning inhibiton in fresh-cut pears. Strawberry tree extracts (leaves and branches) revealed higher total phenolic content (207.97 ± 0.01 mg GAE.gNES−1 and 104.07 ± 16.38 mg GAE.gNES−1, respectively), confirmed by the plethora of phenolic compounds identified by LC-ESI-UHR-QqTOF-HRMS and quantified by HPLC. This phytochemical composition was reflected in the low IC50 against PPO and POX obtained. Despite the lower phenolic content (6.76 ± 0.11 mg GAE.gNES−1) and antioxidant activity (IC50 = 45.59 ± 1.34 mg mL−1), apple byproduct extract showed potential in delaying browning. This study highlights the opportunity of byproducts and agricultural wastes extracts as novel anti-browning agents.


2012 ◽  
Vol 7 (6) ◽  
pp. 1092-1100 ◽  
Author(s):  
Renata Grubešić ◽  
Dario Kremer ◽  
Marijana Končić ◽  
Jadranka Rodríguez ◽  
Marko Randić

AbstractThe content of biologically active phenolic compounds (total polyphenols, tannins, flavonoids, and phenolic acids) were determined using spectrophotometry in four wild Croatian species of Daphne L. in the family Thymelaeaceae (Daphne alpina, D. cneorum, D. laureola, and D. mezereum). The concentration of total flavonoids (TF) was highest in the leaves of these Daphne species (0.12–0.51% dry herb weight, DW) whereas the content of other phenolic compounds analyzed were highest in the roots, including total polyphenols (TP; 2.71–19.03% DW), tannins (T; 1.14–7.39% DW), and total phenolic acids (TPA; 0.12–0.87% DW). D. alpina contained the highest amount of polyphenols, with the exception of flavonoids, where maximum concentrations were found in D. laureola. We also examined the antioxidant activity of leaf, stem, and root extracts. All extracts analyzed demonstrated high free radical scavenging activity with the highest concentration in the leaf extracts of D. alpina. Leaf extracts of D. cneorum showed the highest antioxidant activity in a β-carotene bleaching assay.


2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.


Author(s):  
Rodrigo Zunta Raia ◽  
Ana Paula De Souza ◽  
Beatriz Cervejeira Bolanho Barros

The effect of three different drying temperatures (60, 70, 80 ºC) was evaluated in effective diffusivity, colorimetric parameters, antioxidant activity, and betalain content of pulp (P) and mesocarp (M) of pitaya (Hylocereus costaricensis). The increase in drying temperatures had a direct influence on drying constants, showing the drying rate is faster when drying temperature of 80 ºC was used. Modified Page model described the drying behaviour of pitaya pulp and mesocarp. In the colorimetric parameters, the higher drying temperatures decreased the luminosity and the reddish tonality and increased the color saturation. The drying promoted a concentration of antioxidants compounds in relation to in natura samples. The betalain content decreased in the pulp and it was maintained in the mescocarp samples, while the total phenolic compounds and the antioxidant activity increased in both pitaya parts when higher temperatures were used in the drying process. For these reasons the temperature of 60 ºC was indicating the most appropriate temperature for temperature for pitaya pulp end mesocarp drying process.


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