scholarly journals Laser Light Treatment of Seeds for Improving the Biomass Photosynthesis, Chemical Composition and Biological Activities of Lemongrass Sprouts

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 478
Author(s):  
Mohammad K. Okla ◽  
Mohamed A. El-Tayeb ◽  
Ahmed Ali Qahtan ◽  
Mostafa A. Abdel-Maksoud ◽  
Yahya B. Elbadawi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Organic Acids ◽  
Essential Oils ◽  
Laser Treatment ◽  
Laser Light ◽  
Light Treatment ◽  
Chemical Compositions ◽  
Fresh Weight ◽  
Primary And Secondary Metabolites

Compared to seeds and mature plants, sprouts are well characterized based on their nutritive values and biological properties. Moreover, laser light application is known to be a promising approach to improving plant growth, photosynthesis, and nutraceutical values. However, no studies have investigated the phytochemicals and biological activity of lemongrass (Cymbopogon proximus (Hochst. ex A.Rich.) Chiov.) sprouts or the further improvement of their quality by applying laser light treatment. We carried out a preliminary experiment for the optimization of laser treatment conditions, finding that a helium neon (He–Ne) laser at 632 nm and 5 mW for 5 min provided the most favorable conditions. We then investigated fresh weight, photosynthetic reactions, and primary and secondary metabolites, including sugars, amino acids, organic acids, essential oils, and phenolic compounds. Moreover, we studied the effect of laser light-induced changes in chemical compositions on the antioxidant, anti-diabetic, and anti-cholesterol activities of Cymbopogon proximus sprouts grown from laser-treated seeds. Laser light treatment increased the photosynthesis and respiration and hence the fresh weight of Cymbopogon proximus sprouts. Overall, sprouting increased most bioactive primary and secondary metabolites as compared to seeds. Increased photosynthesis by laser light improved carbon allocation and raised non-structural carbohydrates, which in turn led to improved synthesis of amino acids, organic acids, and essential oils, as well as phenolic and flavonoid compounds. As a result, laser light significantly improved the antioxidant capacity in terms of increasing the levels of ferric reducing antioxidant power (FRAP) (from 9.5 to 21 µmole trolox/g fresh weight (FW)), oxygen radical absorbance (ORAC) (from 400 to 1100 µmole trolox/100 g FW), and DPPH (from 5% to 25% of inhibation) and enhanced the hypocholesterolemic and antidiabetic activity through increasing the percentage of cholesterol micellar solubility (CMS) inhibition (from 42% to 62%) and glycemic index (from 33 to 17 µmole/g) over sprouts and seeds. In conclusion, the synergism of seed laser treatment and sprouting induced the health-promoting bioactive compounds in Cymbopogon proximus as compared to seeds, which can be applied at a large scale to improve the biochemical, physiological, and nutraceutical values of medicinal and crop sprouts.

scholarly journals Profiles of Secondary Metabolites (Phenolic Acids, Carotenoids, Anthocyanins, and Galantamine) and Primary Metabolites (Carbohydrates, Amino Acids, and Organic Acids) during Flower Development in Lycoris radiata

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 248
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Ye Jin Kim ◽  
Bao Van Nguyen ◽  
Ye Eun Park ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Organic Acids ◽  
Phenolic Acids ◽  
Flower Development ◽  
Developmental Stages ◽  
Tca Cycle ◽  
Primary And Secondary Metabolites ◽  
Hydrophilic Compounds ◽  
Tca Cycle Intermediates

This study aimed to elucidate the variations in primary and secondary metabolites during Lycorisradiata flower development using high performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). The result showed that seven carotenoids, seven phenolic acids, three anthocyanins, and galantamine were identified in the L. radiata flowers. Most secondary metabolite levels gradually decreased according to the flower developmental stages. A total of 51 metabolites, including amines, sugars, sugar intermediates, sugar alcohols, amino acids, organic acids, phenolic acids, and tricarboxylic acid (TCA) cycle intermediates, were identified and quantified using GC-TOFMS. Among the hydrophilic compounds, most amino acids increased during flower development; in contrast, TCA cycle intermediates and sugars decreased. In particular, glutamine, asparagine, glutamic acid, and aspartic acid, which represent the main inter- and intracellular nitrogen carriers, were positively correlated with the other amino acids and were negatively correlated with the TCA cycle intermediates. Furthermore, quantitation data of the 51 hydrophilic compounds were subjected to partial least-squares discriminant analyses (PLS-DA) to assess significant differences in the metabolites of L. radiata flowers from stages 1 to 4. Therefore, this study will serve as the foundation for a biochemical approach to understand both primary and secondary metabolism in L. radiata flower development.

scholarly journals Unani Perspective and New Researches of Sa’ad ku’fi (Cyperus rotundus): A Review

2018 ◽  
Vol 8 (6) ◽  
pp. 378-381
Author(s):  
Hana Abbasi ◽  
Hifzul Kabir
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Cyperus Rotundus ◽  
Nootropic Agent ◽  
Major Chemical ◽  
Important Medicinal Plant ◽  
Different Parts

Cyperus rotundus L. (cyperaceae) is an important medicinal plant which is used in traditional medicine particularly in Unani system of medicine. It is commonly known as motha which is a lawn grass and grows easily in tropical, subtropical and temperate regions. Major Chemical constituents enclosed with this herb are flavonoids, tannins, glycosides, furochromones, monoterpenes, sesquiterpenes, sitosterol, alkaloids, saponins, terpenoids, essential oils, starch, carbohydrates, protein, separated amino acids and many other secondary metabolites. Different parts of cyperus rotundus have been reported to possess multiple pharmacological actions like antibacterial, anticancerous, anticonvulsant, antidiabetic, antidiarrheal, antigenotoxic, anti-inflammatory, antilipidemic, antimalarial, antimutagenic, antiobesity, antioxidant, anti-uropathogenic, hepatoprotective, cardioprotective, neuroprotective, and nootropic agent. Keywords: Unani, sa’ad, flavonoids, sesquiterpenes, antibacterial, antilipidemic.

Microbial Products and Their Role in Soil Health and Sustainable Agriculture

2021 ◽  
pp. 181-204
Author(s):  
Amrendra Kumar ◽  
Swati Agarwal
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Organic Acids ◽  
Sustainable Agriculture ◽  
Single Cell ◽  
Enzyme Inhibitors ◽  
Soil Health ◽  
Commercial Production ◽  
Economic Sector ◽  
Microbial Products

Microbial products are being used from ages in known as well as unknown forms. Some common products harvested from microbes include proteins, amino acids, antibiotics, antibodies, secondary metabolites, organic acids, lipids, and so on. It also includes antivirals, polymers, surfactants, enzyme inhibitors, nutraceuticals, and many industrial and agricultural products. Moreover, sometimes the whole single celled microbes are harvested as a rich source of protein called single cell proteins. In a nutshell, all these products cover almost every economic sector like food, feed, agriculture, healthcare, fuel, textile, and pharmaceutical. Hence, these microbial products have serious socio-economic impressions and have unleashed enormous possibilities in terms of commercial production. However, only a small fraction of microbial products are exploited, and a larger chest remains to be achieved. In the chapter, the importance of microbes in the production of proteins, enzymes, and secondary metabolites are discussed in detail with special emphasis on sustainable agriculture.

scholarly journals Comparative Metabolic Profiling of Grape Pulp during the Growth Process Reveals Systematic Influences under Root Restriction

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 377
Author(s):  
Feng Leng ◽  
Shuyan Duan ◽  
Shiren Song ◽  
Liping Zhao ◽  
Wenping Xu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
High Performance ◽  
Developmental Stages ◽  
Grape Berry ◽  
Vitis Vinifera L ◽  
Pulp Tissue ◽  
Primary And Secondary Metabolites ◽  
Amino Acids And Derivatives ◽  
Root Restriction

The compositions and contents of metabolites in the pulp tissue play critical roles in the fruit quality for table grape. In this study, the effects of root restriction (RR) on the primary and secondary metabolites of pulp tissue at five developmental stages were studied at the metabolomics level, using “Red Alexandria” grape berry (Vitis vinifera L.) as materials. The main results were as follows: 283 metabolites were annotated by using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS); 28 and 16 primary metabolites contents were increased and decreased, and 11 and 19 secondary metabolites contents were increased and decreased, respectively, along the berry development; RR significantly decreased 12 metabolites (four amino acids and derivatives, three organic acids, four flavonoids and one other compound) contents, and improved 40 metabolites (22 amino acids and derivatives, six nucleotides, four carbohydrates, four cofactors, three cinnamic acids and one other compound) accumulation at the different developmental stages. Altogether, our study would be helpful to increase our understanding of grape berry’s responses to RR stress.

scholarly journals The Effect of Different Drying Methods on Primary and Secondary Metabolites in Korean Mint Flower

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 698
Author(s):  
Chang Ha Park ◽  
Hyeon Ji Yeo ◽  
Chanung Park ◽  
Yong Suk Chung ◽  
Sang Un Park
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
High Performance ◽  
Freeze Drying ◽  
Sinapic Acid ◽  
Drying Methods ◽  
Oven Drying ◽  
Primary And Secondary Metabolites ◽  
Freeze Dried ◽  
Β Carotene

Edible flowers have been used in the food and beverage industries because of their high nutritional value, flavor, and scent. For the storage of edible flowers used in these industries, drying is a necessity to store the materials more easily and prevent the damage of metabolites in the flowers. However, drying may affect metabolite retention because drying conditions can differ according to the various methods. In this study, Agastache rugosa flowers were dried using four different methods (oven drying at 25 ± 1 °C, 50 ± 1 °C, 80 ± 1 °C, and freeze drying) and primary and secondary metabolites were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography time-of-flight mass spectrometry (GC-TOF/MS). Freeze-dried flower samples contained higher levels of carotenoids (lutein, 13Z-β-carotene, β-carotene, and 9Z-β-carotene) and phenolics (rosmarinic acid, ferulic acid, and sinapic acid). Contrarily, the 80 °C oven-dried flower samples contained higher levels of most amino acids and flavonoids (including acacetin and tilianin) and at 25 °C and 50 °C contained higher levels of carbohydrates. Therefore, freeze-drying is a suitable method for retaining carotenoids and phenolics. In contrast, oven drying at 50 °C was highly recommended to retain amino acids and flavonoids.

scholarly journals Seaweeds as Source of Bioactive Substances and Skin Care Therapy—Cosmeceuticals, Algotheraphy, and Thalassotherapy

Cosmetics ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 68 ◽  
Author(s):  
Leonel Pereira
Keyword(s):  
Amino Acids ◽  
Secondary Metabolites ◽  
Sea Water ◽  
Environmental Pollutants ◽  
Normal Growth ◽  
Bioactive Substances ◽  
Temperature Changes ◽  
Primary And Secondary Metabolites ◽  
First Results ◽  
Bioactive Agents

Riverine, estuarine, and coastal populations have always used algae in the development of home remedies that were then used to treat diverse health problems. The empirical knowledge of various generations originated these applications, and their mechanism of action is, in most cases, unknown, that is, few more scientific studies would have been described beyond simple collection and ethnographic recording. Nevertheless, recent investigations, carried out with the purpose of analyzing the components and causes that alter the functioning and the balance of our organism, are already giving their first results. Water, and especially sea water is considered as essential to life on our planet. It sings all the substances necessary and conducive to the development of the living being (minerals, catalysts, vitamins, amino acids, etc.). Oceans cover over 70% of Earth, being home to up to 90% of the organisms in the planet. Many rich resources and unique environments are provided by the ocean. Additionally, bioactive compounds that multiple marine organisms have a great potential to produce can be used as nutraceuticals, pharmaceuticals, and cosmeceuticals. Both primary and secondary metabolites are produced by algae. The first ones are directly implicated in development, normal growth, or reproduction conditions to perform physiological functions. Stress conditions, like temperature changes, salinity, environmental pollutants, or UV radiation exposure cause the performance of secondary metabolites. In algae, proteins, polysaccharides, fatty acids, and amino acids are primary metabolites and phenolic compounds, pigments, vitamins, sterols, and other bioactive agents, all produced in algae tissues, are secondary metabolites. These algal active constituents have direct relevance in cosmetics.

scholarly journals Profiles of Essential Oils and Correlations with Phenolic Acids and Primary Metabolites in Flower Buds of Magnolia heptapeta and Magnolia denudata var. purpurascens

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 221
Author(s):  
Hyejin Hyeon ◽  
Ho Bong Hyun ◽  
Boram Go ◽  
Sung Chun Kim ◽  
Yong-Hwan Jung ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Essential Oils ◽  
Phenolic Acids ◽  
Herbal Medicines ◽  
White Flower ◽  
Flower Buds ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Metabolic Systems ◽  
Magnolia Denudata

Magnolia flower buds are a source of herbal medicines with various active compounds. In this study, differences in the distribution and abundance of major essential oils, phenolic acids, and primary metabolites between white flower buds of Magnolia heptapeta and violet flower buds of Magnolia denudata var. purpurascens were characterised. A multivariate analysis revealed clear separation between the white and violet flower buds with respect to primary and secondary metabolites closely related to metabolic systems. White flower buds contained large amounts of monoterpene hydrocarbons (MH), phenolic acids, aromatic amino acids, and monosaccharides, related to the production of isoprenes, as MH precursors, and the activity of MH synthase. However, concentrations of β-myrcene, a major MH compound, were higher in violet flower buds than in white flower buds, possibly due to higher threonine levels and low acidic conditions induced by comparatively low levels of some organic acids. Moreover, levels of stress-related metabolites, such as oxygenated monoterpenes, proline, and glutamic acid, were higher in violet flower buds than in white flower buds. Our results support the feasibility of metabolic profiling for the identification of phytochemical differences and improve our understanding of the correlated biological pathways for primary and secondary metabolites.

scholarly journals Assessment of phytochemicals and quantification of primary and secondary metabolites of Artabotrys hexapetalus (L.f.) Bhandari leaves

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2099-2103
Author(s):  
Kousalya P ◽  
Doss VA
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Secondary Metabolites ◽  
Petroleum Ether ◽  
Primary Metabolites ◽  
Primary And Secondary Metabolites ◽  
Potential Source

The main goal of the research was to explore the existence of phytochemicals, quantification of primary and secondary metabolites of leaves extract of Artabotrys hexapetalus  (L.f.) Bhandari. The phytochemical activity of leaves of Artabotrys hexapetalus was assessed using different solvent extracts like water, ethanol, acetone, chloroform and petroleum ether. Among the different solvent extracts, aqueous leaves extract revealed the high content of phytochemicals. So the aqueous leaves extract was used for further investigations. Aqueous leaves extract of Artabotrys hexapetalus was subjected to quantitative analysis of primary metabolites like carbohydrates, proteins and amino acids. Quantitative analysis of secondary metabolites like flavonoids, tannins and phenols were performed using aqueous leaves extract of Artabotrys hexapetalus. Qualitative screening of phytochemicals reported the existence of carbohydrates, amino acids, proteins, flavonoids, alkaloids, saponins, phenols, glycosides, tannins and diterpenes. Quantitative analysis showed the presence of carbohydrates (43.16±1.0 mg/g extract), proteins (60.4±0.88 mg/g extract), amino acids (19.33 ± 1.30 mg/g extract), flavonoids (28.3 ±0.91 mg/g extract), tannins (24.53±1.02 mg/g extract) and phenols (7.63±0.85 mg/g extract). The present study concluded that aqueous leaves extract of Artabotrys hexapetalus as a potential source of phytochemicals, primary and secondary metabolites.

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