Response of Lawn Grasses to Salinity Stress and Protective Potassium Effect

The salinity effects on lawn grasses caused by mine salts (halite and carnallitite) due to road de-icing processes was the aim of this study. Biometric and physiological parameters were evaluated after salt dosage of 50 and 100 g m−2 applied to a lawn surface twice and four times, in weekly intervals. The alleviating effect to the salinity on the grasses from potassium enriched soil was also evaluated. Protective effect of potassium included mostly plasma membrane integrity and an increase in the level of photosynthetic pigments. This probably resulted in more efficient photosynthesis and thus increased lawn growth. Simultaneously, only a slight reduction in relative water content (RWC) was noted, so the recorded increase in proline level may indicate its participation in osmotic adjustment. Our results confirm the importance of proper, and even over-optimal, potassium fertilization of lawn grasses exposed to salinity. Moreover, it is advisable to use other fossil salts instead of halite for the de-icing of near-green areas. The mined salt carnallitite which, besides NaCl, contains about 30% of carnalite (KCl·MgCl2·6H2O) could be such a substance.

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Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

Reproduction Abstracts ◽

10.1530/repabs.1.p248 ◽

2014 ◽

Author(s):

Mello Papa Patricia de ◽

Carlos Ramires Neto ◽

Priscilla Nascimento Guasti ◽

Rosiara Rosaria Dias Maziero ◽

Yame F R Sancler-Silva ◽

...

Keyword(s):

Plasma Membrane ◽

Membrane Integrity ◽

Plasma Membrane Integrity ◽

Frozen Semen

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Plasma membrane integrity in health and disease: significance and therapeutic potential

Cell Discovery ◽

10.1038/s41421-020-00233-2 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Catarina Dias ◽

Jesper Nylandsted

Keyword(s):

Plasma Membrane ◽

Therapeutic Potential ◽

Calcium Influx ◽

Membrane Integrity ◽

Cell Types ◽

Physical Parameters ◽

Membrane Repair ◽

Plasma Membrane Integrity ◽

Repair Mechanisms ◽

And Function

AbstractMaintenance of plasma membrane integrity is essential for normal cell viability and function. Thus, robust membrane repair mechanisms have evolved to counteract the eminent threat of a torn plasma membrane. Different repair mechanisms and the bio-physical parameters required for efficient repair are now emerging from different research groups. However, less is known about when these mechanisms come into play. This review focuses on the existence of membrane disruptions and repair mechanisms in both physiological and pathological conditions, and across multiple cell types, albeit to different degrees. Fundamentally, irrespective of the source of membrane disruption, aberrant calcium influx is the common stimulus that activates the membrane repair response. Inadequate repair responses can tip the balance between physiology and pathology, highlighting the significance of plasma membrane integrity. For example, an over-activated repair response can promote cancer invasion, while the inability to efficiently repair membrane can drive neurodegeneration and muscular dystrophies. The interdisciplinary view explored here emphasises the widespread potential of targeting plasma membrane repair mechanisms for therapeutic purposes.

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Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm

Fertility and Sterility ◽

10.1016/j.fertnstert.2007.02.003 ◽

2008 ◽

Vol 89 (1) ◽

pp. 223-227 ◽

Cited By ~ 8

Author(s):

Man Mohan Misro ◽

Sankar Prasad Chaki

Keyword(s):

Plasma Membrane ◽

Laboratory Test ◽

Membrane Integrity ◽

Human Sperm ◽

Plasma Membrane Integrity ◽

Test Kit

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Plasma membrane integrity: implications for health and disease

BMC Biology ◽

10.1186/s12915-021-00972-y ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Dustin A. Ammendolia ◽

William M. Bement ◽

John H. Brumell

Keyword(s):

Plasma Membrane ◽

Membrane Damage ◽

Membrane Integrity ◽

Whole Body ◽

Plasma Membrane Integrity ◽

Plasma Membrane Damage ◽

Cellular Environment ◽

Health And Disease ◽

Repair Pathways ◽

The Impact

AbstractPlasma membrane integrity is essential for cellular homeostasis. In vivo, cells experience plasma membrane damage from a multitude of stressors in the extra- and intra-cellular environment. To avoid lethal consequences, cells are equipped with repair pathways to restore membrane integrity. Here, we assess plasma membrane damage and repair from a whole-body perspective. We highlight the role of tissue-specific stressors in health and disease and examine membrane repair pathways across diverse cell types. Furthermore, we outline the impact of genetic and environmental factors on plasma membrane integrity and how these contribute to disease pathogenesis in different tissues.

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Arabidopsis Synaptotagmin1 Maintains Plasma Membrane Integrity

The Plant Cell ◽

10.1105/tpc.108.201211 ◽

2008 ◽

Vol 20 (12) ◽

pp. 3182-3182 ◽

Cited By ~ 1

Author(s):

Nancy A. Eckardt

Keyword(s):

Plasma Membrane ◽

Membrane Integrity ◽

Plasma Membrane Integrity

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Assessment of the Functional Integrity of Hepatocytes: A Brief Review

Alternatives to Laboratory Animals ◽

10.1177/026119299302100303 ◽

1993 ◽

Vol 21 (3) ◽

pp. 324-329

Author(s):

Jeffrey R. Fry ◽

Alison H. Hammond

Keyword(s):

Plasma Membrane ◽

Trypan Blue ◽

Membrane Integrity ◽

Blue Dye ◽

Plasma Membrane Integrity ◽

Functional Integrity ◽

Cellular Integrity

A variety of approaches to assessment of cellular integrity exist, based on tests of integrity of the plasma membrane, tests of metabolic competence, and asessment of morphology. By definition, such approaches address different aspects of cellular integrity and hence are not interchangeable indices of cellular integrity. Accordingly, it would be most appropriate to characterise hepatocyte preparations on the basis of more than just trypan blue dye exclusion (a test of plasma membrane integrity) as is customary. A scheme for the choice of the most appropriate mix of tests of cellular integrity is presented.

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Toxic and osmotic effects of glycerol on human granulocytes

AJP Cell Physiology ◽

10.1152/ajpcell.1984.247.5.c382 ◽

1984 ◽

Vol 247 (5) ◽

pp. C382-C389 ◽

Cited By ~ 28

Author(s):

W. J. Armitage ◽

P. Mazur

Keyword(s):

Plasma Membrane ◽

Osmotic Stress ◽

Cell Volume ◽

Membrane Integrity ◽

Fluorescein Diacetate ◽

Major Fraction ◽

Plasma Membrane Integrity ◽

Human Granulocytes ◽

Osmotic Effects

Human granulocytes are damaged by exposure to concentrations of glycerol as low as 0.5 M. We therefore investigated the addition of glycerol to granulocytes and its subsequent dilution under various conditions to try to distinguish between toxic and harmful osmotic effects of glycerol. The lesion caused by glycerol at 0 degree C was expressed as a loss of plasma membrane integrity (as visualized by fluorescein diacetate) only after incubation (greater than or equal to 1 h) at 37 degrees C. This damage was not ameliorated when osmotic stress was lessened by reducing the rates of addition and dilution of glycerol to keep the computed cell volume within 80-170% of isotonic cell volume. However, when osmotic stress was reduced further by increasing the temperature of addition and dilution of glycerol from 0 degree C to 22 degrees C, the tolerance of the cells to 1 M glycerol increased somewhat. Reducing exposure to glycerol to 3 min or less at 0 degree C greatly increased survival, but this time was too short to allow glycerol to equilibrate intracellularly. Finally, the presence of extra impermeant solute (NaCl or sucrose) in the medium to reduce the equilibrium cell volume to 60% of isotonic cell volume enabled granulocytes to survive 30-min exposure to 1 M glycerol at 0 degree C, but cells had to remain shrunken during the 37 degrees C incubation to prevent the loss of membrane integrity. Suspensions that contained damaged granulocytes formed aggregates when incubated at 37 degrees C, and these aggregates were responsible for a major fraction of the observed loss in viability.

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Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

Acta Biologica Hungarica ◽

10.1556/018.67.2016.2.1 ◽

2016 ◽

Vol 67 (2) ◽

pp. 125-132 ◽

Cited By ~ 2

Author(s):

Szabolcs Tamás Nagy ◽

Balázs Kakasi ◽

László Pál ◽

Máté Havasi ◽

Miklós Bercsényi ◽

...

Keyword(s):

Plasma Membrane ◽

Ambient Temperature ◽

Membrane Integrity ◽

High Ambient Temperature ◽

Mitochondrial Activity ◽

Plasma Membrane Integrity ◽

Flow Cytometric ◽

Fish Sperm ◽

Sperm Plasma Membrane ◽

Flow Cytometric Study

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Efek Penambahan Vitamin E dalam Pengencer Glukosa Fosfat terhadap Daya Tahan Hidup Spermatozoa Domba pada Suhu 5 °C

Sains Peternakan ◽

10.20961/sainspet.9.1.25-31 ◽

2017 ◽

Vol 9 (1) ◽

pp. 25

Author(s):

Nilawati Widjaya

Keyword(s):

Plasma Membrane ◽

Vitamin E ◽

Analysis Of Variance ◽

Membrane Integrity ◽

Plasma Membrane Integrity ◽

Glucose Phosphate ◽

Randomized Design ◽

Completely Randomized Design

The purpose of this study was to know the effect of vitamin E addition in glucose phosphate diluent on the survival of sheep spermatozoa at 5 °C. The xperiment used 3 rams. The design used was Completely Randomized Design (CRD) with 5 treatments and 4 replicates, each replicate with 3 ejaculate cement of sheep. The treatments were the dose of Vitamin E with E0 = 0 μg/ml, E1 = 1 μg/ml, E2 = 4 μg/ml, E3 = 7 μg/ml, and E4 = 10 μg/ml. Variables measured were motility, live spermatozoa and plasma membrane integrity. Data were analyzed by using analysis of variance according to Steel and Torrie (1981). The result showed that treatment of vitamin E with a dose of 1 μg/ml - 10 μg/ml l had no significant effect on motility, percentage live spermatozoa and membrane integrity of sheep spermatozoa on day two (P> 0.05).

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