scholarly journals Chemical and Microbial Characterization of Washed Rice Water Waste to Assess Its Potential as Plant Fertilizer and for Increasing Soil Health

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2391
Author(s):  
Abba Nabayi ◽  
Christopher Teh Boon Sung ◽  
Ali Tan Kee Zuan ◽  
Tan Ngai Paing ◽  
Nurul Izzati Mat Akhir
Keyword(s):  
Bacterial Species ◽  
Soil Health ◽  
Nutrient Content ◽  
Nutrient Concentrations ◽  
Total N ◽  
Available N ◽  
Microbial Characterization ◽  
Rice Water ◽  
Water Waste

The wastewater from washed rice water (WRW) is often recommended as a source of plant nutrients in most Asian countries, even though most current research on WRW lack scientific rigor, particularly on the effects of rice washing intensity, volumetric water-to-rice ratio (W:R), and condition of the WRW before plant application. This research was thus carried out: (1) to determine how various rice washing intensities, fermentation periods (FP), and W:R would affect the nutrient content in WRW, and (2) to isolate, identify, and characterize the bacterial community from fermented WRW. The WRW was prepared at several rice washing intensities (50, 80, and 100 rpm), FP (0, 3, 6, and 9 days), and W:R (1:1, 3:1, and 6:1). The concentrations of all elements (except P, Mg, and Zn) and available N forms increased with increasing FP and W:R. Beneficial N-fixing and P- and K-solubilizing bacteria were additionally detected in WRW, which helped to increase the concentrations of these elements. Monovalent nutrients -N, , and K are soluble in water. Thus, they were easily leached out of the rice grains and why their concentrations increased with W:R. The bacteria population in WRW increased until 3 days of fermentation, then declined, possibly because there was an insufficient C content in WRW to be a source of energy for bacteria to support their prolonged growth. While C levels in WRW declined over time, total N levels increased then decreased after 3 days, where the latter was most possibly due to the denitrification and ammonification process, which had led to the increase in -N and . The optimum FP and W:R for high nutrient concentrations and bacterial population were found to be 3 to 9 days and 3:1 to 6:1, respectively. WRW contained nutrients and beneficial bacterial species to support plant growth.

scholarly journals Fermentation of Washed Rice Water Increases Beneficial Plant Bacterial Population and Nutrient Concentrations

2021 ◽  
Vol 13 (23) ◽  
pp. 13437
Author(s):  
Abba Nabayi ◽  
Christopher Teh Boon Sung ◽  
Ali Tan Kee Zuan ◽  
Tan Ngai Paing
Keyword(s):  
Indole Acetic Acid ◽  
Bacterial Population ◽  
Soil Health ◽  
Nutrient Content ◽  
Nutrient Concentrations ◽  
N Fixation ◽  
Bacterial Strains ◽  
Iaa Production ◽  
Promotional Effect ◽  
Rice Water

Washed rice water (WRW) is said to be a beneficial plant fertilizer because of its nutrient content. However, rigorous scientific studies to ascertain its efficiency are lacking. The purpose of this study was to determine the effect of fermenting WRW on the bacterial population and identification, and to measure how fermentation affects the nutrient composition of WRW. Rice grains were washed in a volumetric water-to-rice ratio of 3:1 and at a constant speed of 80 rpm for all treatments. The treatments were WRW fermented at 0 (unfermented), 3, 6, and 9 days. Bacterial N fixation and P and K solubilization abilities in the fermented WRW were assessed both qualitatively and quantitatively. The isolated bacterial strains and the WRW samples were also tested for catalase and indole acetic acid (IAA) production ability. Significantly greater N fixation, P and K solubilization, and IAA production were recorded after 3 days of fermentation compared with other fermentation periods, with increases of 46.9–83.3%, 48.2–84.1%, 73.7–83.6%, and 13.3–85.5%, respectively, in addition to the highest (2.12 × 108 CFU mL−1) total bacterial population. Twelve bacteria strains were isolated from the fermented WRW, and the gene identification showed the presence of beneficial bacteria Bacillus velezensis, Enterobacter spp., Pantoea agglomerans, Klebsiella pneumoniae and Stenotrophomonas maltophilia at the different fermentation periods. All the identified microbes (except Enterobacter sp. Strain WRW-7) were positive for catalase production. Similarly, all the microbes could produce IAA, with Enterobacter spp. strain WRW-10 recording the highest IAA of up to 73.7% higher than other strains. Generally, with increasing fermentation periods, the nutrients N, S, P, K, Mg, NH4+, and NO3− increased, while pH, C, and Cu decreased. Therefore, fermentation of WRW can potentially increase plant growth and enhance soil health because of WRW’s nutrients and microbial promotional effect, particularly after 3 days of fermentation.

Nutritional value of wheat and corn distiller’s dried grain with solubles: Digestibility and digestible contents of energy, amino acids and phosphorus, nutrient excretion and growth performance of grower-finisher pigs

2007 ◽  
Vol 87 (1) ◽  
pp. 103-114 ◽  
Author(s):  
G. P. Widyaratne ◽  
R. T. Zijlstra
Keyword(s):  
Amino Acids ◽  
Growth Performance ◽  
Nutritional Value ◽  
Control Diet ◽  
Nutrient Content ◽  
Total N ◽  
Nutrient Excretion ◽  
Ileal Digestibility ◽  
Corn Ddgs

Two experiments were conducted to evaluate the nutritional value of distiller’s dried grains with solubles (DDGS) samples derived from corn, wheat and a wheat/corn blend (4:1). Specifically, the digestibility and digestible contents of energy, amino acids (AA) and P, N and P excretion, and growth performance were determined in grower-finisher pigs. In exp. 1, 12 ileal cannulated barrows (64.6 ± 6.4 kg) had restricted access (2.6 × maintenance) to a wheat-control diet or one of three diets containing 40% DDGS sample of corn, wheat or wheat/corn origin that replaced wheat. For energy, apparent total tract digestibility was highest for wheat (85%; P < 0.05) and did not differ among the DDGS samples (77 to 79%; P > 0.10). Total tract digestible energy (DE) was higher for corn DDGS (4292 kcal kg-1 DM; P < 0.05) than wheat/corn DDGS, wheat DDGS and wheat samples (4038, 4019 and 3807). For lysine, apparent ileal digestibility (AID) was highest for wheat (71%; P < 0.05) and did not differ among DDGS samples (59 to 63%; P > 0.10). The AID lysine content was highest for corn DDGS (0.51% DM; P < 0.05), intermediate for wheat/corn DDGS and wheat DDGS (0.45 and 0.42), and lowest for wheat (0.37%). For P, total tract digestibility was lowest for wheat (15%; P < 0.05) and did not differ among DDGS samples (53 to 56%; P > 0.10). Total N excretion was highest for wheat/corn DDGS and wheat DDGS (55 and 58 g d-1; P < 0.05), intermediate for corn DDGS (44) and lowest for wheat (36). Total P excretion did not differ among DDGS (11 g d-1) and was lowest for wheat (8; P < 0.05). In exp. 2, 100 pigs (52.0 ± 3.3 kg) were fed a wheat-pea control diet or one of three diets containing 25% of the three DDGS samples (3.375 Mcal DE kg-1; 2.50 g SID lysine Mcal-1 DE) for 5 wk. Overall, average daily feed intake (ADFI) and daily gain (ADG) were higher for pigs fed the wheat control diet than the DDGS-containing diets (P < 0.05), but feed efficiency did not differ (P > 0.10). In summary, the digestible nutrient content of wheat DDGS is lower than corn DDGS and higher than wheat. Followin g pre-characterization of digestible nutrient profile, feeding DDGS reduced growth performance indicating that further research is required to improve the nutritional value of DDGS. Key words: Distiller's dried grains with solubles, pig, digestibility, energy, amino acid

scholarly journals Effects of boron and vermicompost on growth, yield and nutrient content of Chilli (Capsicum annum L.)

2021 ◽  
Vol 6 (1) ◽  
pp. 31-36
Author(s):  
KS Nawrin ◽  
MJ Uddin ◽  
AHMZ Ali ◽  
MK Rahman
Keyword(s):  
Leaf Area ◽  
Growth Yield ◽  
Dry Weight ◽  
Nutrient Content ◽  
Growth And Yield ◽  
Nutrient Concentrations ◽  
Nutrient Accumulation ◽  
Total N ◽  
Capsicum Annum ◽  
Total P

The effects of boron (B) and vermicompost (VC) on growth and yield of Chilli (Capsicum annum L.) and nutrient accumulation in its fruits was examined. The highest plant height (22 cm), leaf number per plant (73), leaf area (502.53 cm2/plant), dry weight (22.27g/plant), fruit length (8.97cm), fruit number per plant (6), fruit yield (11.76 g/plant) were recorded in B0.5 kg/ha + VC5 ton/ha at harvest. The results of growth and yield of Chilli varied significantly (p<0.05) and increased with time. The total nutrient concentrations in the fruits were measured and varied significantly (p<0.05). The highest concentration of total P (0.028 %), K (2.50%), S (0.20 %), Cu (8.0 mg/kg), Fe (410 mg/kg) and Mn (0.80 mg/kg) in the fruit were observed in B0.5 kg/ha +VC5 ton/ha treatment and total N (0.41 %) and Zn (3.50 mg/kg) were found in B1.5 kg/ha + VC5 ton/ha treatment. The overall best growth, yield and nutrient accumulation in the fruits of Chilli was achieved in B0.5 kg/ha + VC5 t/ha treatment. J. Biodivers. Conserv. Bioresour. Manag. 2020, 6(1): 31-36

Nitrogen accretion, soil fertility, and Douglas-fir nutrition in association with redstem ceanothus

1983 ◽  
Vol 13 (1) ◽  
pp. 122-125 ◽  
Author(s):  
Dan Binkley ◽  
Lynn Husted
Keyword(s):  
Nutrient Content ◽  
Douglas Fir ◽  
Soil N ◽  
Total N ◽  
Available N ◽  
Nutrient Contents ◽  
Mixed Plantations ◽  
Attractive Candidate ◽  
Foliar Nutrient ◽  
Total Soil N

Nutrient contents of soil and foliage of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) saplings were determined in areas without redstem ceanothus (Ceanothussanguineus Pursh), at the edges of redstem ceanothus patches, and within patches. Tree growth was not examined. Total soil N (0–15 cm depth) was 50–75% greater at the edge of and within redstem patches than in areas without redstem. Assuming soil N was initially similar at all three positions, N accretion was 24–50 kg•ha−1•year−1 for 10 years. Total N accretion in the ecosystem was probably 45–80 kg•ha−1•year−1. Available N index and extractable Ca and Mg were 2–3 times greater at the edges of and within the redstem patches than outside them. Concentrations of N, Ca, and Mg in the Douglas-fir foliage were lowest outside the patches, intermediate at the edges, and highest within the patches. Because needle weight was lower for saplings within patches than for those at the edges, the latter had the greatest foliar nutrient content. Enhancement of site fertility by redstem ceanothus, as well as its benefits to wildlife and slope stability, make it an attractive candidate for mixed plantations with conifers.

scholarly journals Morphometric and Nutritional Characterization of the Main Spanish Lentil Cultivars

Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 741
Author(s):  
Javier Plaza ◽  
M. Remedios Morales-Corts ◽  
Rodrigo Pérez-Sánchez ◽  
Isabel Revilla ◽  
Ana M. Vivar-Quintana
Keyword(s):  
Scientific Evidence ◽  
Nutrient Content ◽  
Mediterranean Area ◽  
Western Mediterranean ◽  
Protein Food ◽  
Content Assessment ◽  
Protein Levels ◽  
Nutritional Characteristics ◽  
Clear Differentiation

Nowadays, there is a growing demand for high-quality vegetal protein food products, such as pulses and lentils in particular. However, there is no scientific evidence on the nutritional and morphometric characterization of the main lentil cultivars in the Western Mediterranean area. For this reason, the aim of this work is to carry out a morphometric and nutritional characterization of the main Spanish lentil cultivars. Nutrient content assessment was performed on dry matter. The results showed that all studied cultivars are large and heavy lentils, except for the cultivar “Pardina”. They have high protein levels, ranging from 21% to 25%, which is higher than those found in any other pulse, as well as a high carbohydrate content, greater than 59% in all cases. Fiber content was higher than expected in “Armuña” and “Rubia Castellana” cultivars, ranging from 6% to 6.6%, and exceptionally high in the case of the cultivar “Pardina”, which reached 7.8%. Conversely, very low values were found for fat content, varying between 0.5% and 0.9%. Ca, Fe and Mg levels were remarkably higher (from 550 ppm to 851 ppm, from 98 ppm to 139 ppm and from 790 ppm to 989 ppm, respectively) than those found for other lentil cultivars, especially the high Mg content in the cultivars “Jaspeada” and “Microjaspeada”, both above 955 ppm. Clear differentiation was found between the cultivars “Rubia Castellana”, “Pardina” and those included in the Protected Geographical Indication (PGI) “Lenteja de la Armuña”. Overall, lentil cultivars included in the PGI “Lenteja de la Armuña” showed better morphometric and nutritional characteristics than cultivars “Pardina” or “Rubia Castellana”.

scholarly journals Biofilm Formation in Xanthomonas arboricola pv. pruni: Structure and Development

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 546
Author(s):  
Pilar Sabuquillo ◽  
Jaime Cubero
Keyword(s):  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Environmental Changes ◽  
Nutrient Content ◽  
Infection Process ◽  
Bacterial Attachment ◽  
Key Factors ◽  
Cell Structures ◽  
Microscopy Techniques

Xanthomonasarboricola pv. pruni (Xap) causes bacterial spot of stone fruit and almond, an important plant disease with a high economic impact. Biofilm formation is one of the mechanisms that microbial communities use to adapt to environmental changes and to survive and colonize plants. Herein, biofilm formation by Xap was analyzed on abiotic and biotic surfaces using different microscopy techniques which allowed characterization of the different biofilm stages compared to the planktonic condition. All Xap strains assayed were able to form real biofilms creating organized structures comprised by viable cells. Xap in biofilms differentiated from free-living bacteria forming complex matrix-encased multicellular structures which become surrounded by a network of extracellular polymeric substances (EPS). Moreover, nutrient content of the environment and bacterial growth have been shown as key factors for biofilm formation and its development. Besides, this is the first work where different cell structures involved in bacterial attachment and aggregation have been identified during Xap biofilm progression. Our findings provide insights regarding different aspects of the biofilm formation of Xap which improve our understanding of the bacterial infection process occurred in Prunus spp and that may help in future disease control approaches.

scholarly journals Nanopore-Sequencing Characterization of the Gut Microbiota of Melolontha melolontha Larvae: Contribution to Protection against Entomopathogenic Nematodes?

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 396
Author(s):  
Ewa Sajnaga ◽  
Marcin Skowronek ◽  
Agnieszka Kalwasińska ◽  
Waldemar Kazimierczak ◽  
Karolina Ferenc ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Entomopathogenic Nematodes ◽  
Bacterial Species ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Nanopore Sequencing ◽  
Bacterial Phyla ◽  
Melolontha Melolontha

This study focused on the potential relationships between midgut microbiota of the common cockchafer Melolontha melolontha larvae and their resistance to entomopathogenic nematodes (EPN) infection. We investigated the bacterial community associated with control and unsusceptible EPN-exposed insects through nanopore sequencing of the 16S rRNA gene. Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes were the most abundant bacterial phyla within the complex and variable midgut microbiota of the wild M. melolontha larvae. The core microbiota was found to include 82 genera, which accounted for 3.4% of the total number of identified genera. The EPN-resistant larvae differed significantly from the control ones in the abundance of many genera belonging to the Actinomycetales, Rhizobiales, and Clostridiales orders. Additionally, the analysis of the microbiome networks revealed different sets of keystone midgut bacterial genera between these two groups of insects, indicating differences in the mutual interactions between bacteria. Finally, we detected Xenorhabdus and Photorhabdus as gut residents and various bacterial species exhibiting antagonistic activity against these entomopathogens. This study paves the way to further research aimed at unravelling the role of the host gut microbiota on the output of EPN infection, which may contribute to enhancement of the efficiency of nematodes used in eco-friendly pest management.

scholarly journals Construction and Characterization of a proU-gfp Transcriptional Fusion That Measures Water Availability in a Microbial Habitat

2002 ◽  
Vol 68 (9) ◽  
pp. 4604-4612 ◽  
Author(s):  
Catherine A. Axtell ◽  
Gwyn A. Beattie
Keyword(s):  
Pseudomonas Syringae ◽  
Water Availability ◽  
Water Deprivation ◽  
Bacterial Species ◽  
Transcriptional Fusion ◽  
Bacterial Biosensor ◽  
E Coli ◽  
Quantitative Manner ◽  
Microbial Habitat

ABSTRACT We constructed and characterized a transcriptional fusion that measures the availability of water to a bacterial cell. This fusion between the proU promoter from Escherichia coli and the reporter gene gfp was introduced into strains of E. coli, Pantoea agglomerans, and Pseudomonas syringae. The proU-gfp fusion in these bacterial biosensor strains responded in a quantitative manner to water deprivation caused by the presence of NaCl, Na2SO4, KCl, or polyethylene glycol (molecular weight, 8000). The fusion was induced to a detectable level by NaCl concentrations of as low as 10 mM in all three bacterial species. Water deprivation induced proU-gfp expression in both planktonic and surface-associated cells; however, it induced a higher level of expression in the surface-associated cells. Following the introduction of P. agglomerans biosensor cells onto bean leaves, the cells detected a significant decrease in water availability within only 5 min. After 30 min, the populations were exposed, on average, to a water potential equivalent to that imposed by approximately 55 mM NaCl. These results demonstrate the effectiveness of a proU-gfp-based biosensor for evaluating water availability on leaves. Furthermore, the inducibility of proU-gfp in multiple bacterial species illustrates the potential for tailoring proU-gfp-based biosensors to specific habitats.

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