scholarly journals Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2464
Author(s):  
Félix Morán ◽  
Ester Marco-Noales ◽  
Elena Landeras ◽  
Montserrat Roselló ◽  
Adela Abelleira ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Agricultural Sector ◽  
Bacterial Species ◽  
Genetic Lineage ◽  
The European Union ◽  
Genetic Lineages ◽  
The North ◽  
Molecular Approaches ◽  
Leaf Spots ◽  
Different Populations

Currently, kiwifruit cultivation arouses great economic interest in the agricultural sector in several countries of the European Union due to high consumer demand and good results achieved in terms of production potential and fruit quality. One of the main bacterial species that cause yield losses in kiwifruit plants is Pseudomonas syringae. Diseases such as bacterial canker, caused by pathovar (pv.) actinidiae; floral bud necrosis caused by pv. syringae and leaf spots caused by pv. actinidifoliorum (Pfm) are clear examples. Between 2014 and 2017, in the main kiwifruit producing areas in the north and east of Spain, several surveys were carried out in search of these pathogens. Analyses realized from symptomatic and asymptomatic plants of Actinidiadeliciosa revealed the existence of new bacterial isolates close to Pfm. These new isolates werelow virulence pathogens similar to Pfm but belonging to a new group of P. syringae that affected the leaves of A. chinensis var. deliciosa. This study focused on the characterization and classification of these new isolates by a polyphasic approach in order to provide more information for understanding how the different populations of P.syringae affecting kiwifruit. They had the phenotypic characteristics of Pfm but by molecular approaches, they constituted a supported genetic lineage closely-related to Pfm independent of the five lineages described so far. This work revealed the great diversity found in P. syringae species affecting kiwifruit plants and supports the hypothesis that Pfm is a low virulence pathogen which is long established in Europe.

Nitrogen pollution in the European Union – an economy-environment confrontation

1996 ◽  
Vol 23 (3) ◽  
pp. 198-206 ◽  
Author(s):  
Ester van der Voet ◽  
René Kleijn ◽  
Helias A. Udo de Haes
Keyword(s):  
Agricultural Sector ◽  
Flow Analysis ◽  
Major Change ◽  
Environmental Problems ◽  
The European Union ◽  
Agricultural Efficiency ◽  
The North ◽  
Vegetable Products ◽  
Anthropogenic Nitrogen ◽  
N Compounds

SummaryThe production and import of fertilizer have previously been identified as the major source of three separate European environmental problems related to nitrogen compounds. In order to obtain a picture of the changes in the anthropogenic nitrogen flows needed for a solution, the effectiveness of several more radical interventions is estimated using Substance Flow Analysis (SFA). A selection of rigorous technical measures is expected to reduce the atmospheric deposition of N compounds to a level that seems acceptable for the EU as a whole, and will reduce the anthropogenic input of N compounds into the North Sea well below the agreed level of 50% of the present load. The third problem, the pollution of groundwater with nitrates, would remain unsolved. Resolution of this last problem requires radical changes in the agricultural sector, reducing agricultural output substantially. Two directions are explored by assessing the impacts of the extremes: termination of industrial fertilizer use to combat the problems at the source, and abolition of the entire stock-breeding sector to increase agricultural efficiency radically. These changes do contribute to a solution of the groundwater problem, but imply either greater dependence on imported food, or a major change in food consumption patterns, from animal towards vegetable products. The current EU population and diet are incompatible with the absence of environmental problems. The question then arises, how does acceptance of the draconian measures required to solve such an environmental problem weigh up against acceptance of the fact of having to cope with contaminated groundwater?

scholarly journals Farms in the system of European supportpolicy

2008 ◽  
pp. 79-83
Author(s):  
Dóra Nagyné Demeter
Keyword(s):  
Agricultural Policy ◽  
Common Agricultural Policy ◽  
Agricultural Sector ◽  
The European Union ◽  
Family Farms ◽  
Member States ◽  
Economic Changes ◽  
The North ◽  
Structure Changes ◽  
Agricultural Structure

Common Agricultural Policy (CAP) has been having a great past, it is over numerous direction and structure changes in the last half century. After the Treaty of Rome, the harmonization of he agricultural structure and the production of Member States has been launched, during which the economic changes in the world and in Europe were continuously being kept track of. By using common experience, they strived to develop an agricultural sector that supplies the Member States of the Community with food, while the competitiveness and living circumstances of family farms are being improved. The general directions outlined in Stresa in 1958 were significantly redrawn owing to the transformed market conditions and EU accessions. The subsidization of family farms and the improvement of their profitability are having a stressed importance nowadays, too. Our country, as the fully qualified member of the European Union tries to meet its obligations undertaken in the accession treaty and therefore to develop an agricultural sector carrying out diversified agricultural production with a healthy age structure. The aim of the study is to specifically overview the issue of common agricultural policy by stressing the main development points and to introduce the Hungarian conditions of family farms. Besides, there will be an evaluation of the research works examining the income conditions of family farms in the North Great Plain region.

Effect of a protectant copper application on Psa infection of kiwifruit trap plants

2017 ◽  
Vol 70 ◽  
pp. 310-314
Author(s):  
J.L. Tyson ◽  
S.J. Dobson ◽  
M.A. Manning
Keyword(s):  
New Zealand ◽  
Disease Control ◽  
Pseudomonas Syringae ◽  
Low Risk ◽  
Bacterial Canker ◽  
Complete Protection ◽  
Leaf Spots ◽  
Copper Compounds ◽  
Trap Plants

Pseudomonas syringae pv. actinidiae (Psa) causes bacterial canker of kiwifruit, which is an ongoing threat to New Zealand kiwifruit production. Disease control depends on orchard practices such as removal of visibly diseased material, pruning during low-risk periods, and the application of foliar bactericides. Although the use of copper compounds on Actinidia species (kiwifruit) can cause phytotoxicity, copper-based formulations remain a key component of Psa control in New Zealand. The effect of single copper applications on Psa infection of ‘Hort16A’ trap plants was studied over the Spring of 2014 (Sept—Nov). Psa leaf spots were observed at the beginning of October, appearing first on the untreated plants. Although the copper sprays did not achieve complete protection, particularly as the inoculum built up during November, the copper-sprayed plants always had less disease than the untreated plants.

scholarly journals Economic and financial position of agricultural enterprises in the period before the accession to the European Union

2012 ◽  
Vol 50 (No. 11) ◽  
pp. 486-494
Author(s):  
Z. Chrastinová
Keyword(s):  
European Union ◽  
Agricultural Sector ◽  
Agricultural Land ◽  
Agricultural Products ◽  
Cold Weather ◽  
Business Success ◽  
Agricultural Cooperatives ◽  
The European Union ◽  
Adverse Weather ◽  
Legal Entities

In the year before the accession to the European Union, the Slovak agricultural sector reported a loss of SKK 2.4 billion and following a profitable year, the earnings were reduced by SKK 2.8 billion. The situation was caused by a number of reasons, namely reduced sales of agricultural products, damage resulting from adverse weather effects (cold weather, hail, drought and  swine fever), as well as widening of the price gap compared to the year before (increasing input prices in agriculture and decreasing purchase prices of agricultural products, especially in livestock production). Legal entities and natural persons experienced mixed business success. While 51% of legal entities made profit, the figure rose to 76% in the group of natural persons. Both the agricultural cooperatives and trading companies performed with a loss. The loss per hectare of agricultural land (a.l.) was substantially lower in the case of business companies. Natural persons - private farmers were profitable over the period. The gap between the profitable and loss-making enterprises has widened. Some 60% of profitable enterprises owned by legal entities made only a small profit below SKK 0.5 million. The loss-making performance was typical for more productive areas of Slovakia. This was related to stronger effects of adverse climate in 2003.

scholarly journals Economic and Environmental Consequences of the ECJ Genome Editing Judgment in Agriculture

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1212
Author(s):  
Alexander Gocht ◽  
Nicola Consmüller ◽  
Ferike Thom ◽  
Harald Grethe
Keyword(s):  
Genome Editing ◽  
Agricultural Sector ◽  
Ghg Emissions ◽  
Food Products ◽  
Food Prices ◽  
Farm Income ◽  
The European Union ◽  
Environmental Consequences ◽  
European Court ◽  
The Eu

Genome-edited crops are on the verge of being placed on the market and their agricultural and food products will thus be internationally traded soon. National regulations, however, diverge regarding the classification of genome-edited crops. Major countries such as the US and Brazil do not specifically regulate genome-edited crops, while in the European Union, they fall under GMO legislation, according to the European Court of Justice (ECJ). As it is in some cases impossible to analytically distinguish between products from genome-edited plants and those from non-genome-edited plants, EU importers may fear the risk of violating EU legislation. They may choose not to import any agricultural and food products based on crops for which genome-edited varieties are available. Therefore, crop products of which the EU is currently a net importer would become more expensive in the EU, and production would intensify. Furthermore, an intense substitution of products covered and not covered by genome editing would occur in consumption, production, and trade. We analyzed the effects of such a cease of EU imports for cereals and soy in the EU agricultural sector with the comparative static agricultural sector equilibrium model CAPRI. Our results indicate dramatic effects on agricultural and food prices as well as on farm income. The intensification of EU agriculture may result in negative net environmental effects in the EU as well as in an increase in global greenhouse gas (GHG) emissions. This suggests that trade effects should be considered when developing domestic regulation for genome-edited crops.

scholarly journals The North Caucasus Region as a Blind Spot in the “European Green Deal”: Energy Supply Security and Energy Superpower Russia

Energies ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 17
Author(s):  
José Antonio Peña-Ramos ◽  
Philipp Bagus ◽  
Dmitri Amirov-Belova
Keyword(s):  
European Union ◽  
Energy Supply ◽  
Blind Spot ◽  
Illustrative Case ◽  
The European Union ◽  
North Caucasus ◽  
Strong Argument ◽  
The North ◽  
The Impact ◽  
Energy Supply Security

The “European Green Deal” has ambitious aims, such as net-zero greenhouse gas emissions by 2050. While the European Union aims to make its energies greener, Russia pursues power-goals based on its status as a geo-energy superpower. A successful “European Green Deal” would have the up-to-now underestimated geopolitical advantage of making the European Union less dependent on Russian hydrocarbons. In this article, we illustrate Russian power-politics and its geopolitical implications by analyzing the illustrative case of the North Caucasus, which has been traditionally a strategic region for Russia. The present article describes and analyses the impact of Russian intervention in the North Caucasian secessionist conflict since 1991 and its importance in terms of natural resources, especially hydrocarbons. The geopolitical power secured by Russia in the North Caucasian conflict has important implications for European Union’s energy supply security and could be regarded as a strong argument in favor of the “European Green Deal”.

scholarly journals Molecular Analysis of SARS-CoV-2 Genetic Lineages in Jordan: Tracking the Introduction and Spread of COVID-19 UK Variant of Concern at a Country Level

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 302
Author(s):  
Malik Sallam ◽  
Azmi Mahafzah
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Rapid Evolution ◽  
Molecular Signature ◽  
Special Focus ◽  
Genetic Lineage ◽  
Synonymous Mutation ◽  
Spike Gene ◽  
Genetic Lineages ◽  
The Uk

The rapid evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is manifested by the emergence of an ever-growing pool of genetic lineages. The aim of this study was to analyze the genetic variability of SARS-CoV-2 in Jordan, with a special focus on the UK variant of concern. A total of 579 SARS-CoV-2 sequences collected in Jordan were subjected to maximum likelihood and Bayesian phylogenetic analysis. Genetic lineage assignment was undertaken using the Pango system. Amino acid substitutions were investigated using the Protein Variation Effect Analyzer (PROVEAN) tool. A total of 19 different SARS-CoV-2 genetic lineages were detected, with the most frequent being the first Jordan lineage (B.1.1.312), first detected in August 2020 (n = 424, 73.2%). This was followed by the second Jordan lineage (B.1.36.10), first detected in September 2020 (n = 62, 10.7%), and the UK variant of concern (B.1.1.7; n = 36, 6.2%). In the spike gene region, the molecular signature for B.1.1.312 was the non-synonymous mutation A24432T resulting in a deleterious amino acid substitution (Q957L), while the molecular signature for B.1.36.10 was the synonymous mutation C22444T. Bayesian analysis revealed that the UK variant of concern (B.1.1.7) was introduced into Jordan in late November 2020 (mean estimate); four weeks earlier than its official reporting in the country. In Jordan, an exponential increase in COVID-19 cases due to B.1.1.7 lineage coincided with the new year 2021. The highest proportion of phylogenetic clustering was detected for the B.1.1.7 lineage. The amino acid substitution D614G in the spike glycoprotein was exclusively present in the country from July 2020 onwards. Two Jordanian lineages dominated infections in the country, with continuous introduction/emergence of new lineages. In Jordan, the rapid spread of the UK variant of concern should be monitored closely. The spread of SARS-CoV-2 mutants appeared to be related to the founder effect; nevertheless, the biological impact of certain mutations should be further investigated.

scholarly journals Construction and Characterization of a proU-gfp Transcriptional Fusion That Measures Water Availability in a Microbial Habitat

2002 ◽  
Vol 68 (9) ◽  
pp. 4604-4612 ◽  
Author(s):  
Catherine A. Axtell ◽  
Gwyn A. Beattie
Keyword(s):  
Pseudomonas Syringae ◽  
Water Availability ◽  
Water Deprivation ◽  
Bacterial Species ◽  
Transcriptional Fusion ◽  
Bacterial Biosensor ◽  
E Coli ◽  
Quantitative Manner ◽  
Microbial Habitat

ABSTRACT We constructed and characterized a transcriptional fusion that measures the availability of water to a bacterial cell. This fusion between the proU promoter from Escherichia coli and the reporter gene gfp was introduced into strains of E. coli, Pantoea agglomerans, and Pseudomonas syringae. The proU-gfp fusion in these bacterial biosensor strains responded in a quantitative manner to water deprivation caused by the presence of NaCl, Na2SO4, KCl, or polyethylene glycol (molecular weight, 8000). The fusion was induced to a detectable level by NaCl concentrations of as low as 10 mM in all three bacterial species. Water deprivation induced proU-gfp expression in both planktonic and surface-associated cells; however, it induced a higher level of expression in the surface-associated cells. Following the introduction of P. agglomerans biosensor cells onto bean leaves, the cells detected a significant decrease in water availability within only 5 min. After 30 min, the populations were exposed, on average, to a water potential equivalent to that imposed by approximately 55 mM NaCl. These results demonstrate the effectiveness of a proU-gfp-based biosensor for evaluating water availability on leaves. Furthermore, the inducibility of proU-gfp in multiple bacterial species illustrates the potential for tailoring proU-gfp-based biosensors to specific habitats.

The EU's Economic Cooperation with North Korea: The Possibility as a Useful Tool to Complement Korea-US Cooperation

2009 ◽  
Vol 12 (2) ◽  
pp. 125-149 ◽  
Author(s):  
Jong-Sue Lee
Keyword(s):  
European Union ◽  
Human Rights ◽  
Korean Peninsula ◽  
North Korea ◽  
The United States ◽  
Policy Position ◽  
The European Union ◽  
Member States ◽  
The North ◽  
The Eu

North Korea conducted 2nd nuclear test on May 25, 2009. It made a vicious circle and continued military tension on the Korean Peninsula. North Korea regime got a question on the effectiveness of the six party talks and ‘security-economy exchange model’. In addition, the North Korea probably disappointed about the North Korea issue has been excluded from the Obama administration's policy position. So the dialogue or relationship recovery with the United States and North Korea through six-party talks or bilateral talks will be difficult for the time being. This paper examines the EU policy on North Korea. Based on the results, analyzes the EU is likely to act as a balancer on the Korean Peninsula. Through the procedure of deepening and expanding the economic and political unification, the EU utilizes their cooperative policies towards North Korea as an ideal opportunity to realize their internal value and to confirm the commonness within the EU members. The acceleration of the EU's unification, however, began to focus on human rights, and this made their official relationship worse. Yet, the EU is continuously providing food as wells as humanitarian and technological support to North Korea regardless of the ongoing nuclear and human rights issues in North Korea. Also, the number of multinational corporations investing in North Korea for the purpose of preoccupying resources and key industries at an individual nation's level has been increasing. The European Union has unique structure which should follow the way of solving the problem of member states like subsidiary principle. It appears to conflict between normative power of the European Union and strategic interests on member states. This paper examines if the European Union is useful tool to complement Korea-US cooperation in the near future.

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