Zinc Oxide Nanoparticles Boosts Phenolic Compounds and Antioxidant Activity of Capsicum annuum L. during Germination

The effects of zinc oxide nanoparticles on seed germination and seedling growth of Capsicum annuum L. were determined in this research. Total phenols content, total flavonoids, and condensed tannins, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant capacity was determined. Results indicated that treatment with zinc oxide nanoparticles (ZnO-NPs) improved seed germination rate during the first seven days. The seed vigor germination increased 123.50%, 129.40% and 94.17% by treatment with ZnO-NPs suspensions at 100, 200 and 500 ppm, respectively. The morphological parameters tested revealed that ZnO-NPs treatments did not significantly affect plumule development, but they had a significant impact (p ≤ 0.01) on radicle length. Suspensions at 100, 200 and 500 ppm of ZnO-NPs inhibited seedling radicle growth and promoted accumulation of phenolic compounds, with a phytotoxic effect in this organ. Results suggested that zinc oxide nanoparticles influence seed vigor and seedling development and promoted the accumulation of desirable phenolic compounds in the radicle.

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Zinc Oxide and Zinc Oxide Nanoparticles Impact on In Vitro Germination and Seedling Growth in Allium cepa L.

Materials ◽

10.3390/ma13122784 ◽

2020 ◽

Vol 13 (12) ◽

pp. 2784 ◽

Cited By ~ 2

Author(s):

Alicja Tymoszuk ◽

Jacek Wojnarowicz

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Allium Cepa ◽

Seedling Growth ◽

Zinc Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Allium Cepa L ◽

Germinating Seeds

Zinc oxide nanoparticles (ZnO NPs) are ones of the most commonly manufactured nanomaterials worldwide. They can be used as a zinc fertilizer in agriculture to enhance yielding and to control the occurrence of diseases thanks to its broad antifungal and antibacterial action. The aim of this study was to investigate and compare the effects of ZnO submicron particles (ZnO SMPs) and ZnO NPs on the process of in vitro seed germination and seedling growth in onion (Allium cepa L. ‘Sochaczewska’), and to indicate the potential use of these compounds in onion production. In the experiment, disinfected seeds were inoculated on the modified Murashige and Skoog (MS) medium and poured with ZnO SMP or ZnO NP water suspension, at the concentrations of 50, 100, 200, 400, 800, 1600, and 3200 mg∙L−1. During three successive weeks, the germinating seeds were counted. Germination started most often on the second or third day of in vitro culture. The highest share of germination was recorded for seeds treated with 800 mg∙L−1 ZnO SMPs and ZnO NPs (52% and 56%, respectively). After the application of ZnO SMPs and ZnO NPs at the highest tested concentration (3200 mg∙L−1), the share of germinating seeds was only 19% and 11%, respectively. Interestingly, seedlings obtained from control seeds and seeds treated with ZnO SMPs and ZnO NPs did not differ statistically in terms of length, fresh weight, and dry weight of leaves, and roots. Both ZnO SMPs and ZnO NPs, in the concentration range from 50 to 1600 mg∙L−1, can be used to stimulate the germination process of onion seeds, without negative effects on the further growth and development of seedlings. There were no differences found between the action of ZnO NPs and ZnO SMPs, which suggested that the most important factor influencing seed germination was in fact the concentration of zinc ions, not the particle size.

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Plant-Mediated Green Synthesis of Zinc Oxide Nanoparticles Using Peel Extract of Citrus reticulate for Boosting Seed Germination of Brassica nigra Seeds

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2021.19015 ◽

2021 ◽

Vol 21 (6) ◽

pp. 3573-3579

Author(s):

Muhammad Rafique ◽

Muhammad Sohaib ◽

Rabbia Tahir ◽

M. Bilal Tahir ◽

M. Rizwan

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Zinc Oxide Nanoparticles ◽

Brassica Nigra ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Synthesis Of Nanoparticles ◽

Citrus Reticulate ◽

Peel Extract

High demand of food for rapidly increasing population requires novel but ecofriendly fertilizers. Green reducing and capping agents are being explored to minimize production cost and toxicity of chemicals in synthesis of nanoparticles (NPs) which could be used to increase the production of crops and plants. In present research, Zinc Oxide Nanoparticles (ZnO NPs) are produced by employing an eco-friendly, simple and efficient green route using peel extract of Citrus reticulate. The optical properties of green synthesized ZnO NPs are explored by UV-Visible and Photoluminance spectroscopies where NPs presented 3.21 to 3.13 eV band gap. The morphology and purity of the ZnO NPs are analyzed by scanning electron microscopy (SEM), X-ray diffraction technique (XRD) and energy dispersive X-ray spectroscopy (EDX), respectively. The spherical like ZnO NPs having 23–90 nm size exhibited hexagonal structure with 8.89 to 8.62 nm crystallite size. Fourier transform infrared spectroscopy (FTIR) explores the existence of specific functional groups which are responsible for stabilization, capping and reduction during synthesis of nanoparticles. The green synthesized ZnO NPs are tested for seed germination of Brassica nigra (black mustard) seeds at standard temperature and pressure. The activity shows that germination percentage of the Brassica nigra seeds is enhanced 100% and seedling vigor index 16.45 after treatment with ZnO NPs and can be controlled by the concentration of NPs. Therefore, it can be expected that ZnO NPs can serve as the cost effective and ecofriendly nano-fertilizers in agriculture.

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Biosynthesis of ZnO Nanostructures Using Azadirachta indica Leaf Extract and Their Effect on Seed Germination and Seedling Growth of Tomato: An Eco-Friendly Approach

Journal of Nanoelectronics and Optoelectronics ◽

10.1166/jno.2020.2891 ◽

2020 ◽

Vol 15 (11) ◽

pp. 1412-1422

Author(s):

Nishat Arshi ◽

Y. Prashanthi ◽

Tentu Nageswara Rao ◽

Faheem Ahmed ◽

Shalendra Kumar ◽

...

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Azadirachta Indica ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Leaf Extract ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

The Impact

In this study, we report synthesis of Zinc oxide nanoparticles using simple chemical and green methods. The ZnO nanoparticles were synthesized using leaf extract of Azadirachta indica (neem) as reducing agent. The as obtained product was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray analysis (EDAX) and TEM techniques. XRD analysis confirms that ZnO nanoparticles were crystalline having hexagonal Wurtzite structure with (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0) and (1 1 2) planes. SEM analyses show that the as synthesized ZnO NPs were in the form of agglomerates and no other impurity peak was found in the EDS. TEM analyses confirm that the size of the nanoparticle was approx. 50 nm. Here in, we investigate the effect of chemical and green synthesized zinc oxide nanoparticles on germination and growth of lycopersicum esculentus (tomato) using petri plate seed germination method in loamy sand soil. The impact of concentration of applied ZnO nanoparticles via green synthesis and chemical methods were analyzed. Results revealed that green synthesized Zinc oxide nanoparticles showed maximum growth of seedling as compared to chemically synthesized Zinc oxide nanoparticles, bulk ZnO and control. After 50 days of tomato growth analysis, it was recognized that ZnO NPs can be a good green synthetic fertilizer by increasing shoot length, wet weight, dry weight and yield over conventional control. Hence, green method is found to be more effective.

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Effects of the Morphology, Surface Modification and Application Methods of ZnO-NPs on the Growth and Biomass of Tomato Plants

Molecules ◽

10.3390/molecules25061282 ◽

2020 ◽

Vol 25 (6) ◽

pp. 1282

Author(s):

Eneida A. Pérez Velasco ◽

Rebeca Betancourt Galindo ◽

Luis A. Valdez Aguilar ◽

José A. González Fuentes ◽

Bertha A. Puente Urbina ◽

...

Keyword(s):

Zinc Oxide ◽

Surface Modification ◽

Zinc Oxide Nanoparticles ◽

Germination Rate ◽

Dry Weight ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Tomato Plants ◽

Solanum Lycopersicum L ◽

Application Methods

Benefits of nanotechnology in agriculture include reduced fertilizer loss, improved seed germination rate and increased crops quality and yield. The objective of this research was to evaluate the effects of zinc oxide nanoparticles (ZnO-NPs), at 1500 ppm, on tomato (Solanum lycopersicum L.) growth. ZnO-NPs were synthetized to produce either spherical or hexagonal morphologies. In this research, we also studied two application methods (foliar and drench) and nanoparticles’ (NPs) surface modification with maltodextrin. The results obtained indicate that ZnO-NP-treated tomato plants significantly increased plant height, stem diameter and plant organs (leaves, stem and root) dry weight compared to plants without NP treatment.

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Foliar application of zinc oxide nanoparticles and grafting improves the bell pepper (Capsicum annuum L.) productivity grown in NFT system

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha49212327 ◽

2021 ◽

Vol 49 (2) ◽

pp. 12327

Author(s):

José G. URESTI-PORRAS ◽

Marcelino CABRERA-DE-LA FUENTE ◽

Adalberto BENAVIDES-MENDOZA ◽

Alberto SANDOVAL-RANGEL ◽

Alejandro ZERMEÑO-GONZALEZ ◽

...

Keyword(s):

Zinc Oxide ◽

Capsicum Annuum ◽

Zinc Oxide Nanoparticles ◽

Foliar Application ◽

Fruit Weight ◽

Bell Pepper ◽

Control Treatment ◽

Oxide Nanoparticles ◽

Capsicum Annuum L ◽

Weight Number

The bell pepper (Capsicum annuum L.) is a food vegetable with a high nutritional intake, with rich content in vitamins, minerals and antioxidants. In this study, using nutrient film technique (NFT) system, the effect of the zinc oxide nanoparticles on the micromorphology, histology, physiology and production of the grafted pepper was evaluated. The treatments used were grafted and non-grafted plants, four concentrations (0, 10, 20, 30 mg L-1) of zinc oxide nanoparticles, and the experience was organized in a completely randomized design. An increase in grafted plants was observed in the weight, number and size of fruits in 18.1%, 21.8% and 9.6%, the concentration 30 mg L-1 of nanoparticles statistically affected the weight, number and size 46.9%, 47.7% and 18% compared to the control. The interaction with grafted plants and the treatment of 30 mg L-1 of zinc oxide nanoparticles increased fruit weight, number of fruits and size by 62.60%, 57.69% and 29.17% compared to plants without grafting and the control treatment. These results indicate that the use of grafts and zinc oxide nanoparticles could be used in bell pepper production to increase yield.

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Impact of zinc and zinc oxide nanoparticles on the physiological and biochemical processes in tomato and wheat

Botany ◽

10.1139/cjb-2016-0194 ◽

2017 ◽

Vol 95 (5) ◽

pp. 441-455 ◽

Cited By ~ 9

Author(s):

Rayhaneh Amooaghaie ◽

Maryam Norouzi ◽

Mohammad Saeri

Keyword(s):

Zinc Oxide ◽

Seed Germination ◽

Zinc Oxide Nanoparticles ◽

Growth Parameters ◽

Oxide Nanoparticles ◽

Antioxidant Enzyme Activities ◽

Zno Nps ◽

Biochemical Processes ◽

Germination And Growth ◽

Higher Sensitivity

In this study, the effects of various concentrations of zinc and zinc oxide nanoparticles (nZn, nZnO) were evaluated in tomato and wheat. Results showed that at lower concentrations, nZn and nZnO augmented seed germination and growth parameters, whereas with higher concentrations, the nanoparticles reduced these traits. Zn concentrations corresponding to Zn dissolved (3–23 mg Zn·L−1) from nanoparticles (NPs) did not significantly affect the germination indices in either species. Compared with the bulk counterparts of ZnO, NPs exerted more toxicity on seed germination, growth parameters, and chlorophyll and carotenoid contents, and also increased Zn bioaccumulation more. More often than not, nZnO provoked more adverse symptoms than nZn at equivalent concentrations. In both species, the Zn accumulation in roots and shoots followed the order: Zn2+ ions > nZn > nZnO > bulk ZnO > control. Exposure to 200 mg Zn·L−1 nZn and nZnO increased H2O2 accumulation and malondealdehyde (MDA) levels, which were more pronounced in tomato than wheat. The results suggested that the toxicity of NPs could be due to the particle itself, or from the Zn2+ ions dissolved from NPs. Moreover, nanotoxicity, like other stresses, caused oxidative stress in both plants, and the differences in proline accumulation and the antioxidant enzyme activities of leaves, especially APX activity, at least in part, explained the higher sensitivity of tomato to NPs than wheat.

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Evaluation of the Effect of Nanoparticles Zinc Oxide/Camellia sinensis Complex on the Kidney of Rats Treated with Monosodium Glutamate: Antioxidant and Histological Approaches

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666190522075928 ◽

2019 ◽

Vol 20 (7) ◽

pp. 542-550 ◽

Cited By ~ 4

Author(s):

Nahla S. El-Shenawy ◽

Reham Z. Hamza ◽

Fawziah A. Al-Salmi ◽

Rasha A. Al-Eisa

Keyword(s):

Zinc Oxide ◽

Camellia Sinensis ◽

Zinc Oxide Nanoparticles ◽

Morphological Changes ◽

Monosodium Glutamate ◽

Oxide Nanoparticles ◽

High Dose ◽

Enzymatic Antioxidants ◽

Zno Nps ◽

Tea Extract

Background: Zinc oxide nanoparticles (ZnO NPs) are robustly used biomedicine. Moreover, no study has been conducted to explore the consequence of green synthesis of ZnO NPs with Camellia sinensis (green tea extract, GTE) on kidneys of rats treated with monosodium glutamate (MSG). Methods: Therefore, the objective of the research was designed to explore the possible defensive effect of GTE/ZnO NPs against MSG-induced renal stress investigated at redox and histopathological points. Results: The levels of urea and creatinine increased as the effect of a high dose of MSG, in addition, the myeloperoxidase and xanthine oxidase activates were elevated significantly with the high dose of MSG. The levels of non-enzymatic antioxidants (uric acid, glutathione, and thiol) were decreased sharply in MSG-treated rats as compared to the normal group. Conclusion: The data displayed that GTE/ZnO NPs reduced the effects of MSG significantly by reduction of the level peroxidation and enhancement intracellular antioxidant. These biochemical findings were supported by histopathology evaluation, which showed minor morphological changes in the kidneys of rats.

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Zinc oxide nanoparticles help to enhance plant growth and alleviate abiotic stress: A review

Current Protein and Peptide Science ◽

10.2174/1389203721666201016144848 ◽

2020 ◽

Vol 21 ◽

Author(s):

Mohammad Faizan ◽

Fangyuan Yu ◽

Chen Chen ◽

Ahmad Faraz ◽

Shamsul Hayat

Keyword(s):

Zinc Oxide ◽

Plant Growth ◽

Abiotic Stresses ◽

Zinc Oxide Nanoparticles ◽

Growth And Yield ◽

Oxide Nanoparticles ◽

Main Concern ◽

Negative Effects ◽

Zno Nps ◽

Agricultural Yield

: Abiotic stresses arising from atmosphere change belie plant growth and yield, leading to food reduction. The cultivation of a large number of crops in the contaminated environment is a main concern of environmentalists in the present time. To get food safety, a highly developed nanotechnology is a useful tool for promoting food production and assuring sustainability. Nanotechnology helps to better production in agriculture by promoting the efficiency of inputs and reducing relevant losses. This review examines the research performed in the past to show how zinc oxide nanoparticles (ZnO-NPs) are influencing the negative effects of abiotic stresses. Application of ZnO-NPs is one of the most effectual options for considerable enhancement of agricultural yield globally under stressful conditions. ZnO-NPs can transform the agricultural and food industry with the help of several innovative tools in reversing oxidative stress symptoms induced by abiotic stresses. In addition, the effect of ZnO-NPs on physiological, biochemical, and antioxidative activities in various plants have also been examined properly. This review summarizes the current understanding and the future possibilities of plant-ZnO-NPs research.

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Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽

10.3390/nano11030713 ◽

2021 ◽

Vol 11 (3) ◽

pp. 713

Author(s):

Nina Melnikova ◽

Alexander Knyazev ◽

Viktor Nikolskiy ◽

Peter Peretyagin ◽

Kseniia Belyaeva ◽

...

Keyword(s):

Wound Healing ◽

Zinc Oxide ◽

Bacterial Cellulose ◽

Zinc Oxide Nanoparticles ◽

Wound Dressings ◽

Oxide Nanoparticles ◽

Burn Wound ◽

Zno Nps ◽

Wound Model ◽

And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

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