scholarly journals Effect of Dry-Period Diet on the Performance and Metabolism of Dairy Cows in Early Lactation

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 803
Author(s):  
Julien Soulat ◽  
Emilie Knapp ◽  
Nassim Moula ◽  
Jean-Luc Hornick ◽  
Céline Purnelle ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dairy Cows ◽  
Corn Silage ◽  
Blood Metabolites ◽  
Mixed Diet ◽  
Early Lactation ◽  
Dry Period ◽  
Cholesterol Ratio ◽  
Peripartum Period ◽  
Dry Diet

The objective of this work was to observe the effect of three different dry-period diets on blood metabolites (p = 9) and the production and fatty acid (FA) profile of milk (p = 19) in the peripartum period. In this study, 32 Holstein dairy cows, during their dry period, were divided in 3 different diet groups, as follows: the CONC diet (n = 11) was based on concentrate meal and straw, the CORN diet (n = 11) was based on corn silage, and the MIXED diet (n = 10) was based on corn and grass silages. According to our results, the variations of C18:2n-6, C18:3n-3, non-esterified fatty acid (NEFA), NEFA/cholesterol ratio, and albumin were significantly (p < 0.05) different, according to the dry diet. The dry-period diet also had a significant effect on the concentrations of urea and vitamin B12 in the blood. In early lactation, this work showed that blood metabolites were more sensitive to changes in the dry diet than the production and FA profile of milk.

scholarly journals Influence of Days in Milk and Parity on Milk and Blood Fatty Acid Concentrations, Blood Metabolites and Hormones in Early Lactation Holstein Cows

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2081
Author(s):  
Quynh Chau Dang Van ◽  
Emilie Knapp ◽  
Jean-Luc Hornick ◽  
Isabelle Dufrasne
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Blood Cholesterol ◽  
Blood Metabolites ◽  
Early Lactation ◽  
Milk Fatty Acids ◽  
Lactation Stage ◽  
Protein Milk

The objective of this paper is to study the influence of physiological factors that affect the energy balance, such as lactation stage and parity, on milk yield and composition, milk and blood fatty acid concentrations, blood metabolites and hormones in healthy early lactation cows. Thirty-two Holstein dairy cows from five Belgian commercial farms were followed. The grass silage-based diets fed to cows fell within normal composition ranges typically offered to dairy cows on commercial dairy farms in the region. Milk and blood were sampled at each official milk recording and used for the determination of milk fat and protein, milk and blood fatty acids, blood metabolites and hormones concentrations. The considered period was 7 to 150 days in milk. As lactation progressed, concentrations of milk 18:0 and 18:1c9, as well as blood non-esterified fatty acids and β-hydroxybutyrate, decreased, and those of milk C4–C14, blood cholesterol, triglycerides, insulin and IGF-I increased, agreeing with the extensive mobilization of body reserves in early lactation. Lower concentrations of milk C4–C14 and 16:0 and concomitant higher concentrations of milk 18:0 and 18:1c9 suggest a larger body reserve mobilization in first parity cows compared with greater than or equal to second parity cows. This study confirms that early lactation stage along with parity significantly influence milk fatty acids, such as 18:1, and blood metabolites and hormones, such as NEFA and insulin.

scholarly journals Milk fatty acid composition as an indicator of energy status in Holstein dairy cows

2017 ◽  
Vol 60 (3) ◽  
pp. 205-212 ◽  
Author(s):  
Lana Vranković ◽  
Jasna Aladrović ◽  
Daria Octenjak ◽  
Dušanka Bijelić ◽  
Luka Cvetnić ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Dairy Cows ◽  
Total Cholesterol ◽  
Density Lipoprotein ◽  
Milk Fatty Acid ◽  
Early Lactation ◽  
Energy Status ◽  
Dry Period ◽  
Fa Composition ◽  
Sampling Intervals

Abstract. Transition dairy cows often enter a stage of negative energy balance during which the utilization of energy reserves is reflected in the milk fatty acid (FA) composition. In this study, metabolic status was evaluated by measuring milk FA, ruminal short-chain FA (RSCFA), and serum biochemical parameters in Holstein cows. Samples (milk, rumen contents, and blood) were collected around days 30 (early) and 150 (middle) of lactation, and rumen contents and blood samples were collected 30 days before calving (dry). Fatty acids were extracted and FA composition was determined. Glucose, triacylglycerols, total cholesterol, low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), beta-hydroxybutyric acid (BHB), and non-esterified fatty acid (NEFA) concentrations were determined in serum samples. Lower percentages of saturated FA in milk and higher percentages of monounsaturated FA, polyunsaturated FA, and C18:1n-9 were observed in early lactation compared to mid-lactation. In rumen higher concentrations of propionic acid were determined at mid-lactation compared to early lactation. Acetic and butyric acid concentrations showed no significant differences between sampling intervals. In serum higher glucose concentrations were observed during the dry period and mid-lactation than during early lactation. Lower BHB and higher NEFA concentrations were noted during early lactation compared to mid-lactation and the dry period. Total cholesterol, LDL-C, HDL-C and triacylglycerols showed no significant differences between sampling intervals. The results of the present study suggest that determination of milk FA is a potential indicator of energy status in dairy cows.

The effect of breed, parity and body fatness on the lipolytic response of dairy cows

2002 ◽  
Vol 75 (2) ◽  
pp. 209-219 ◽  
Author(s):  
P. Theilgaard ◽  
N. C. Friggens ◽  
K. H. Sloth ◽  
K. L. Ingvartsen
Keyword(s):  
Fatty Acid ◽  
Dairy Cows ◽  
Diet Composition ◽  
Physiological State ◽  
Low Energy ◽  
Ultrasound Measurement ◽  
Early Lactation ◽  
Dry Period ◽  
Body Fatness ◽  
Stage Of Lactation

AbstractThe objective of this study was to examine the effect of breed, parity and body fatness on the lipolytic response of dairy cows. The lipolytic response was estimated as the plasma non-esterified fatty acid (NEFA) response to an adrenergic challenge. Four challenges per lactation were carried out on 124 Danish Holsteins, 101 Danish Red and 82 Jerseys through consecutive lactations. Within breed, there were two genetic lines. Cows were equally distributed across two feeding treatments, a normal and a low energy total mixed ration. Diet composition was constant throughout lactation. The lipolytic response was significantly affected by breed, parity and stage of lactation. Lipolytic response was greater in early lactation than other stages in lactation (P 0·001). The larger breeds had a higher lipolytic response than Jerseys (P 0·05), and showed an increase in lipolytic response between first and second parity (P 0·05), but not between second and third lactation. Lipolytic response in Jerseys was not significantly affected by parity. No line or feeding treatment effects were observed on the lipolytic response. Ultrasound measurement of the area of subcutaneous backfat was used to estimate effect of body fatness on the lipolytic response. There was an increase in lipolytic response with increasing body fatness (P 0·05) in mid lactation and the dry period. The slope of this was not affected by breed or parity. It was concluded that the lipolytic response of dairy cows, and by implication the responsiveness of the lipid reserves, varies according to breed, parity and physiological state.

scholarly journals CD49f+ mammary epithelial cells decrease in milk from dairy cows stressed by overstocking during the dry period

2017 ◽  
Vol 84 (4) ◽  
pp. 414-417 ◽  
Author(s):  
Mario Baratta ◽  
Silvia Miretti ◽  
Paolo Accornero ◽  
Giovanna Galeati ◽  
Andrea Formigoni ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Dairy Cows ◽  
Differential Expression ◽  
Mammary Epithelial Cells ◽  
Stocking Density ◽  
Mammary Epithelial ◽  
Early Lactation ◽  
Dry Period ◽  
Cytokeratin 18 ◽  
Cell Subpopulations

The work reported in this Research Communication describes the modification in epithelial cell populations during the first and the last month of milking in Holstein Friesian cows that have undergone different management during the dry period, and we report the differential expression of CD49f+ and cytokeratin18+ cell subpopulations. Twenty six cows were randomly divided into 2 balanced groups that were housed at stocking density of either 11 m2 (CTR) or 5 m2 from 21 ± 3 d before the expected calving until calving. Cells collected from milk samples taken in early lactation and late lactation were directly analysed for CD45, CD49f, cytokeratin 14, cytokeratin 18 and cell viability. We observed a differential expression with a significant reduction in CD49f+ (P < 0·01) and cytokeratin 18+ (P < 0·05) cells in early lactation. Differences were still evident in late lactation but were not significant. These observations suggest that mammary epithelial cell immunophenotypes could be associated with different animal management in the dry period and we hypothesise they may have a role as biomarkers for mammary gland function in dairy cows.

Response of early lactation dairy cows to the inclusion of the liver technology product ABN-LiFT in the total mixed ration

2002 ◽  
Vol 2002 ◽  
pp. 197-197
Author(s):  
C A Middlemass ◽  
C M Minter ◽  
M Marsden
Keyword(s):  
Folic Acid ◽  
Vitamin B12 ◽  
Dairy Cows ◽  
Energy Requirement ◽  
High Energy ◽  
Early Lactation ◽  
Dry Period ◽  
Total Mixed Ration ◽  
Methyl Group ◽  
Technology Product

Through the dry period to early lactation the cow goes through a dramatic change in her metabolism. To supply the high energy requirement in early lactation fat supply from the diet and body mobilisation increases substantially. As a result, the liver accumulates fat, the rate of detoxification slows down, ammonia accumulates and there’s a reduced supply of fat, glucose and protein to the udder. This trial was designed to evaluate the response of dairy cows to product called ABN-LiFT a proprietary mixture of B-group vitamins and methyl group donors (rumen protected choline, niacin, vitamin B12, biotin, folic acid and thiamine) designed to reduce the accumulation of triglycerides in the liver and accelerate VLDL export.

Functional analysis of the dairy cow mammary transcriptome between early lactation and mid-dry period

2019 ◽  
Vol 86 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Ye Lin ◽  
He Lv ◽  
Minghui Jiang ◽  
Jinyu Zhou ◽  
Shuyuan Song ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dairy Cows ◽  
Digital Gene Expression ◽  
Mammary Glands ◽  
Differentially Expressed ◽  
Mammary Tissue ◽  
Early Lactation ◽  
Gene Ontology Analysis ◽  
Dry Period ◽  
Molecular Events

AbstractIn this research communication we used digital gene expression (DGE) analysis to identify differences in gene expression in the mammary glands of dairy cows between early lactation and the mid-dry period. A total of 741 genes were identified as being differentially expressed by DGE analysis. Compared with their expression in dry cows, 214 genes were up-regulated and 527 genes were down-regulated in lactating cow mammary glands. Gene Ontology analysis showed that lactation was supported by increased gene expression related to metabolic processes and nutrient transport and was associated with decreased gene expression related to cell proliferation. Pathway mapping using the Kyoto Encyclopedia of Genes and Genomes showed that 579 differentially expressed genes had pathway annotations related to 204 pathways. Metabolic pathway-related genes were the most significantly enriched. Genes and pathways identified by the present study provide insights into molecular events that occur in the mammary gland between early lactation and mid-dry period, which can be used to facilitate further investigation of the mechanisms underlying lactation and mammary tissue remodeling in dairy cows.

scholarly journals Relationship between inflammatory biomarkers and oxidative stress with uterine health in dairy cows with different dry period lengths1

2019 ◽  
Vol 3 (2) ◽  
pp. 607-619
Author(s):  
Novi Mayasari ◽  
Erminio Trevisi ◽  
Annarita Ferrari ◽  
Bas Kemp ◽  
Henk K Parmentier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Negative Energy ◽  
Inflammatory Biomarkers ◽  
Early Lactation ◽  
Dry Period ◽  
Inflammatory Status ◽  
Uterine Health ◽  
Uterine Environment ◽  
And Oxidative Stress

Abstract Earlier studies indicated that the inflammatory status of dairy cows in early lactation could not be fully explained by the negative energy balance (NEB) at that moment. The objective of the present study was to determine relationships between inflammatory biomarkers and oxidative stress with uterine health in dairy cows after different dry period lengths. Holstein–Friesian dairy cows were assigned to one of three dry period lengths (0-, 30-, or 60-d) and one of two early lactation rations (glucogenic or lipogenic ration). Cows were fed either a glucogenic or lipogenic ration from 10-d before the expected calving date. Part of the cows which were planned for a 0-d dry period dried themselves off and were attributed to a new group (0 → 30-d dry period), which resulted in total in four dry period groups. Blood was collected (N = 110 cows) in weeks −3, −2, −1, 1, 2, and 4 relative to calving to determine biomarkers for inflammation, liver function, and oxidative stress. Uterine health status (UHS) was monitored by scoring vaginal discharge (VD) based on a 4-point scoring system (0, 1, 2, or 3) in weeks 2 and 3 after calving. Cows were classified as having a healthy uterine environment (HU, VD score = 0 or 1 in both weeks 2 and 3), nonrecovering uterine environment (NRU, VD score = 2 or 3 in week 3), or a recovering uterine environment (RU, VD score = 2 or 3 in week 2 and VD score= 0 or 1 in week 3). Independent of dry period length, cows with NRU had higher plasma haptoglobin (P = 0.05) and lower paraoxonase levels (P &lt; 0.01) in the first 4 weeks after calving and lower liver functionality index (P &lt; 0.01) compared with cows with HU. Cows with NRU had lower plasma albumin (P = 0.02) and creatinine (P = 0.02) compared with cows with a RU, but not compared with cows with HU. Independent of UHS, cows with a 0 → 30-d dry period had higher bilirubin levels compared with cows with 0-, 30-, or 60-d dry period (P &lt; 0.01). Cows with RU and fed a lipogenic ration had higher levels of albumin in plasma compared with cows with NRU and fed a lipogenic ration (P &lt; 0.01). In conclusion, uterine health was related to biomarkers for inflammation (haptoglobin and albumin) and paraoxonase in dairy cows in early lactation. Cows which were planned for a 0-d dry period, but dried themselves off (0 → 30-d dry period group) had higher bilirubin levels, which was possibly related to a more severe NEB in these cows. Inflammatory biomarkers in dairy cows in early lactation were related to uterine health in this period.

Changes in nitrogen balance of dairy cows during the dry period and early lactation following different dry period diets

1997 ◽  
Vol 1997 ◽  
pp. 94-94
Author(s):  
J.M. Moorby ◽  
S. Miles ◽  
R.T. Evans ◽  
W.J. Fisher ◽  
D.W.R. Davies
Keyword(s):  
Dairy Cows ◽  
Late Pregnancy ◽  
High Protein ◽  
Barley Straw ◽  
Early Lactation ◽  
Dry Period ◽  
Body Protein ◽  
Grass Silage ◽  
Low Protein ◽  
Ad Libitum

Increases in yields of milk and milk protein have been observed from dairy cows offered a high protein supplement during the dry period (Van Saun, Idleman and Sniffen, 1993; Moorby, Dewhurst and Marsden, 1996). One possible mechanism for this is an accumulation of maternal body protein during late pregnancy and its later release during lactation. This experiment was designed to investigate the effect of diet on the potential of dairy cows to accumulate and release body nitrogen over the course of the dry period and the first 20 weeks of lactation.Twelve multiparous Holstein-Friesian cows were offered one of three diets for 6 weeks prior to calving, with 4 animals per diet, in an continuous design experiment. Animals were offered ad libitum access to A) grass silage only (medium protein), B) a grass silage/barley straw mix (60:40 on a dry matter basis) (low protein), or C) grass silage plus 0.5 kg/d high protein maize gluten meal (high protein). After calving, all animals were offered a standard lactation diet based on ad libitum grass silage plus 10 kg/d concentrate to week 12 of lactation, with 7 kg/d thereafter. Animals were housed in individual stalls for 6 d N-balance procedures on three separate occasions in two groups of six animals: during the dry period (at approximately 3 to 4 weeks before calving), early-lactation (weeks 7 to 8 after calving) and mid-lactation (weeks 17 to 18).

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