scholarly journals Association Analysis of Polymorphisms in the 5′ Flanking Region of the HSP70 Gene with Blood Biochemical Parameters of Lactating Holstein Cows under Heat and Cold Stress

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2016
Author(s):  
Zaheer Abbas ◽  
Lirong Hu ◽  
Hao Fang ◽  
Abdul Sammad ◽  
Ling Kang ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Cold Stress ◽  
Biochemical Parameters ◽  
Lipid Peroxide ◽  
Control Group ◽  
Hsp70 Gene ◽  
Nucleotide Polymorphisms ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Heat And Cold Stress

Thermal stress (heat and cold) has large economic and welfare implications for the worldwide dairy industry. Therefore, it is paramount to understand the genetic background of coping mechanism related to thermal stress for the implementation of effective genetic selection schemes in dairy cattle. We performed an association study between 11 single nucleotide polymorphisms having minor allelic frequency (MAF > 0.05) in the HSP70 gene with blood biochemical parameters. The concentrations of growth hormone (GH), lactate (LA), prolactin (PRL), and superoxide dismutase (SOD) in blood were significantly higher (p < 0.05), while the concentrations of blood urea nitrogen (BUN), c-reactive protein (CRP), potassium (K+), lactate dehydrogenase (LDH), lipid peroxide (LPO), and norepinephrine (NE) were significantly lower (p < 0.05) in heat-stressed animals as compared to the control group. A significant (p < 0.05) increase in the concentrations of cortisol (COR), corticosterone (CORT), and potassium (K+) was observed (p < 0.05), while the concentrations of adrenocorticotrophic hormone (ACTH), dopamine (DA), GH, LDH, NE, PRL, and SOD were significantly lower in cold-stressed animals as compared to the control group (p < 0.05). Furthermore, SNP A-12G and C181T were significantly associated with LA (p < 0.05), while A72G was linked with LPO (p < 0.05) in heat-stressed animals. Moreover, the SNPs A-12G and SNP C131G were significantly associated (p < 0.05) with DA and SOD under cold stress condition, respectively. These SNPs markers significantly associated with fluctuations in blood biochemical parameters under thermal stress provide a better insight into the genetic mechanisms underlying climatic resilience in Holstein cattle.

scholarly journals PSXIII-21 Changes of blood biochemical parameters of Chinese Holstein cows in different seasons

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 370-371
Author(s):  
Lirong hu ◽  
Jinhuan Dou ◽  
Gang Guo ◽  
Qing Xu ◽  
Yachun Wang
Keyword(s):  
Cold Stress ◽  
Biochemical Parameters ◽  
Holstein Cows ◽  
Experimental Animals ◽  
Blood Biochemical Parameters ◽  
Chinese Holstein ◽  
Blood Biochemical ◽  
Heat And Cold Stress ◽  
Temperature And Humidity ◽  
Chinese Holstein Cows

Abstract The study was conducted to understand the effect of seasonal change on blood biochemical parameters and verify potential blood biochemical parameters which were selected as candidates for thermal stress response evaluation of Chinese Holstein cows in the previous study using independent individuals. A total of 652 healthy lactating Holstein cows from Sanyuan dairy farm were selected as experimental animals. Their milk production data and blood samples used for detection of blood biochemical parameters, were collected in spring (April), summer (August), fall (November) and winter (January), respectively, as well as the continuous temperature and humidity of cowsheds. The changes in 13 blood biochemical parameters (ACTH, BUN, COR, CRP, GH, GLU, HSP70, LD, LPO, NE, PRL, SOD, T3) among seasons were evaluated by MIXED models in SAS9.2 software. Our results showed that 1) The temperature and humidity index (THI) before sampling for continuous 8 days displayed experimental animals had no stress in spring and fall, but in summer and winter experienced heat and cold stress, respectively; 2) All of above 13 blood biochemical parameters had highly significant difference among seasons (P &lt; 0.01). The concentrations of 8 parameters were significantly higher in summer (P &lt; 0.05), but other 5 parameters were significantly lower (P &lt; 0.05). In winter, 6 parameters were significantly higher (P &lt; 0.05), and other 5 parameters were significant lower (P &lt; 0.05); 3) The changes of 8 parameters (such as GH, PRL) between summer and fall, as well as 6 parameters (such as ACTH, SOD) between winter and fall were consistent as the previous study. These results revealed that heat and cold stress had serious effects on physiological condition of Chinese Holstein cows, and multiple parameters had been confirming to be used as candidates for the evaluation of temperature stress in Holstein cows.

scholarly journals Investigation of Health Hazards in Vehicle Refueling Stations Attendants at Malakand, Khyber Pakhtunkhwa, Pakistan

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Imran Khan ◽  
Saleem Ur Rahman ◽  
Muhammad Ilyas ◽  
Ayaz Ali Khan
Keyword(s):  
Biochemical Parameters ◽  
Blood Parameters ◽  
Control Group ◽  
Blood Biochemical Parameters ◽  
Khyber Pakhtunkhwa ◽  
Blood Biochemical ◽  
Large Size ◽  
Routine Work ◽  
Size Population ◽  
And Control

Vehicles refueling station workers are a class of labors prone to long term petroleum product toxicity due to their routine work at vehicles refueling stations. Health problems posed by the pollutants at the work environment of an individual are closely linked to the nature and level of exposure to these hazardous chemicals. The adverse effect of the toxicants in such environment is a common phenomenon in all the developing countries. The awareness about this problem is lacking in Pakistan, particularly in Khyber Pakhtunkhwa. In present study, blood biochemical parameters like calcium, cholesterol, glucose, magnesium, potassium, phosphorus and triglyceride levels were determined among workers and control individuals. The results of the workers showed an increase in glucose, cholesterol and phosphorus level; while significant decrease was observed in calcium and potassium level as compared to the control group of individuals. While the level of magnesium and triglyceride level was same in both groups. In comparative picture, the blood biochemical parameters were normal in control as that of workers. Further study may be conducted to investigate the effect of such environment on other blood parameters and large size population may be included in the study.

scholarly journals Performance, Biochemical Profile and Antioxidant Activity of Hens Supplemented with Addition of Milk Thistle (Silybum marianum) Seed Cakes in Diet

2019 ◽  
Vol 67 (4) ◽  
pp. 993-1003 ◽  
Author(s):  
Ondřej Šťastník ◽  
Eva Mrkvicová ◽  
Leoš Pavlata ◽  
Andrea Roztočilová ◽  
Barbora Umlášková ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Laying Hens ◽  
Control Group ◽  
Egg Mass ◽  
Milk Thistle ◽  
Feed Mixture ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Experimental Group

The aim of this study was to evaluate the effect of milk thistle seed cakes addition in laying hens diet to performance, blood biochemical parameters and antioxidant activity. A total of 30 Bovans Brown hens were included to the experiment. The trial was performed from the age of 69 weeks to 80 weeks of hens age. The experimental group received feed mixture containing 7% milk thistle seed cakes. Control group received feed mixture without milk thistle seed cakes. After the 69th week of age, the laying hens in the experimental group reached a higher number of eggs and produced more egg mass compared to the control group. In the evaluation of the egg quality parameters, higher Haugh units, a higher millimeter height of the egg and thinner eggshell in the group receiving 7% of the seed cakes were found. When evaluating health indicators, higher antioxidant activity was found in the experimental group. Blood biochemical parameters was without any differences.

scholarly journals Effect of L-glutamic acid on some biochemical parameters of rat blood under the action of sodium nitrite

2016 ◽  
Vol 20 (1) ◽  
pp. 74-77
Author(s):  
N. Salyha ◽  
R. Iskra
Keyword(s):  
Antioxidant Enzymes ◽  
Glutamic Acid ◽  
Sodium Nitrite ◽  
Biochemical Parameters ◽  
Control Group ◽  
Glucose Content ◽  
Rat Blood ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Different Doses

The effect of different doses of L-glutamic acid on activity of antioxidant enzymes and some blood biochemical parameters in rats under the influence of sodium nitrite was studied. It was shown that administration of sodium nitrite leads to decreased activity of antioxidant enzymes and and hemoglobin and glucose content in the blood of animals.It was established, that changes of studied parameters were less expressed in rats administered of L-glutamic acid compared to the control group of animals.

scholarly journals The influence of probiotic additives or multienzyme composition on blood biochemical parameters and milk quality of Lithuanian Black-and-White cattle

2018 ◽  
Vol 70 (3) ◽  
pp. 939-945 ◽  
Author(s):  
J. Dailidavičienė ◽  
R. Budreckienė ◽  
R. Gružauskas ◽  
S. Kerzienė ◽  
V. Andrulevičiūtė ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Biochemical Parameters ◽  
Control Group ◽  
Blood Biochemical Parameters ◽  
Black And White ◽  
Organic Selenium ◽  
Blood Biochemical ◽  
Group A ◽  
Live Yeast

ABSTRACT The aim of the study was to determine the effect of Saccharomyces cerevisiae, multienzyme composition supplementation on milk yield, quality, blood biochemical parameters of Lithuanian-Black-and-White cows. For this reason 28 cows were divided into four groups (three experimental and one control) each with seven cows fed balanced ration (control group) and following experimental groups: addition of 40g supplement of live yeast with organic selenium (group A); 40g supplement of live yeast with aromatic additives (group B); and 0.2g supplement of multienzyme composition (group C) during a 90 days period. The study showed that milk yield was 2.64%, 1.75%, 1.4% higher in groups A, B, C respectively, comparing with the control group. The milk SCC in experimental groups were lower comparing to the control group. The percentage of milk fat was significantly higher in group A - 0.33%, B - 0.31% and C - 0.16% comparing with the control group. All used additives ensure positive dynamics of investigated biochemical parameters in cattle blood. The results indicated that probiotic additives and multienzyme composition supplementation to dairy cows increased cows productivity and milk fat as well. Probiotic additives supplementation decreased SCC values in milk.

scholarly journals Effects of Varying Dietary DL-Methionine Levels on Productive and Reproductive Performance, Egg Quality, and Blood Biochemical Parameters of Quail Breeders

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1839
Author(s):  
Fayiz M. Reda ◽  
Ayman A. Swelum ◽  
Elsayed O.S. Hussein ◽  
Shaaban S. Elnesr ◽  
Ahmad R. Alhimaidi ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Reproductive Performance ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Egg Quality ◽  
Control Group ◽  
Egg Weight ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Kidney Functions

The present study was carried out to study the effects of varying dietary DL-methionine (0, 0.5, 1.5, 2.5, and 3.5 g/kg) levels on the productive and reproductive performance, egg quality and blood biochemical parameters of quail breeders. In total, 150 mature Japanese quails at eight weeks of age were randomly allotted to five groups of 30 for each group. Each group included five replicates, each of six quails (four females and two males). The results showed that egg number, egg weight and egg mass were higher (p < 0.05) with the addition of all DL-methionine levels than that of the control group. Quails from the control group had a lower feed intake (p < 0.001) and a worse feed conversion ratio (FCR) than those from the DL-methionine-treated groups. Supplementation of DL-methionine up to 2.5 g/kg in quail diets increased fertility and hatchability percentages. Birds fed DL-methionine at 1.5 g/kg had the best egg production indices, better FCR and the highest values of fertility and hatchability. Egg weight, yolk %, Haugh unit, egg shape index and unit surface shell weight (USSW) were increased and eggshell % was decreased in quail supplemented with DL-methionine levels compared with the control quail (p < 0.05). Dietary DL-methionine levels did not affect (p > 0.05) the hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs) and packed cell volume (PCV) of quails. DL-methionine levels (0.5 and 2.5 g/kg) augmented lymphocytes and basophile (p < 0.05). Low DL-methionine levels (0.5 or 1.5 g/kg) improved liver enzymes and kidney functions. Dietary DL-methionine levels (except 3.5 g/kg) declined serum lactate dehydrogenase (LDH) and decreased lipid profile parameters (except high-density lipoprotein—HDL). Supplementation of DL-methionine at 0.5 and 1.5 g/kg increased immunoglobulin (IgG, IgM and IgA) and superoxide dismutase (SOD), total antioxidant capacity (TAC), catalase (CAT) and reduced glutathione (GSH) (p < 0.001) compared with the control. In conclusion, dietary supplementation of DL-methionine (1.5 g/kg) can enhance the reproductive performance and egg quality of quail breeders. DL-methionine use at levels of 0.5 or 1.5 g/kg improved the liver and kidney functions, lipid profile, immunity and antioxidant parameters of Japanese quail.

scholarly journals The Effect of Adding Cumin Cyminym Seeds to Concentrated Feed on Some Blood Biochemical Parameters of Black Local Goats

2020 ◽  
Vol 13 (1) ◽  
pp. 101-105
Author(s):  
Amal Awad ◽  
Mohamed Shwayel
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Jugular Vein ◽  
Control Group ◽  
College Of Agriculture ◽  
Blood Samples ◽  
Blood Biochemical Parameters ◽  
The Third ◽  
Blood Biochemical ◽  
Cumin Seed

The current study was conducted the effect of adding cumin cyminym seeds to concentrated feed on some blood biochemical parameters of black local goats. Fifteen female black goats aged 3 years, third milk production season. The study was in a private animal field of the Department of Animal Production- College of Agriculture/ University of Diyala, during the period from 1/11/2018 to 16/4/2019. The goats were divided randomly into three equal groups, 5 for each group. The first group (T1) left without treatment as a control group. The second group (T2) was given 10 g of cumin seed powder to the concentrated feed/ animal per day. The third group (T3) was given 20 g of cumin seed powder to the concentrated feed/ animal per day. Blood samples were collected before the experiment (Zero day) and at the end of the experiment (3 months), 10 ml from jugular vein by via vacationer tubes without anticoagulant. Blood biochemical parameters were also estimated includes: Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides. The results showed no significant (P>0.05) in Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides between (Zero day) and at the end of the experiment (3 months) and between different treated groups during the study period. It was concluded from this study that addition of cumin seed powder to the black local goats ration have no effects on some blood biochemical parameters.

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